Recombinant Human TNFSF10 Protein(Arg115-Gly281)描述别名来源形态序列纯度内毒素水平活性溶解方法储存/保存方法背景说明Accession
| 概述 | |
| 描述 |
Recombinant Human TNF-Related Apoptosis-Inducing Ligand is produced by our E.coli expression system and the target gene encoding Arg115-Gly281 is expressed. |
| 别名 |
TRAIL蛋白；TNFSF10蛋白；CD253蛋白;Tumor Necrosis Factor Ligand Superfamily Member 10; Apo-2 Ligand; Apo-2L; TNF-Related Apoptosis-Inducing Ligand; Protein TRAIL; CD253; TNFSF10; APO2L; TRAIL
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| 来源 |
Escherichia coli.
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| 形态 |
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 250mM Nacl, pH7.5.
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| 性能 | |
| 序列 |
MRERGPQRVAAHITGTRGRSNTLSSPNSKNEKALGRKINSWESSRSGHSFLSNLHLRNGELVIHE KGFYYIYSQTYFRFQEEIKENTKNDKQMVQYIYKYTSYPDPILLMKSARNSCWSKDAEYGLYSIY QGGIFELKENDRIFVSVTNEHLIDMDHEASFFGAFLVG
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| 纯度 |
Greater than 95% as determined by reducing SDS-PAGE.
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| 内毒素水平 |
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
|
| 活性 |
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of themetabolic inhibitor actinomycin D. The ED50 for this effect is 2.5-15 ng/ml.
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| 溶解方法 |
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
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| 储存/保存方法 |
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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| 靶标 | |
| 背景说明 |
Human TNFSF10 is a type II transmembrane protein with an intracellular N-terminus and a ‘TNF homology domain’ (THD) at the extracellular C terminus. TNFSF10 can interact with several distinct receptors. Two of these receptors that belongs to TNFR superfamily, DR4 (TRAIL-R1) and DR5 (TRAIL-R2/TRICK2), are plasma membrane proteins containing intracellular death domains essential for activating apoptosis. TNFSF10 is promising for cancer therapy because it is cytotoxic and activates apoptosis in the majority of malignant cells, but not in normal cells.
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| Accession |
P50591
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