Recombinant human ATP1B1 protein

Recombinant human ATP1B1 protein

货号:
P03726

品牌:
Jinpan

产品简介
基因名 ATP1B1
英文全称 ATPase, Na+/K+ transporting, beta 1 polypeptide
别称 ATP1B
中文名称 钠/钾转运ATPase亚单位β1多肽
Uniprot P05026
GeneID 481
交叉反应(推测) Human Mouse Rat (Rabbit Pig Sheep Gorilla)
氨基酸大小 27kDa
表达氨基酸 58-303aa
Background The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases.Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane.These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle.This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta).The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes.This gene encodes a beta 1 subunit.Alternatively spliced transcript variants encoding different isoforms have been described, but their biological validity is not known.
Application Immunology research
Derived from E.coli
Purification NI-NTA affinity purification
Formulation Lyophilized from a 0.2 μm filtered solution of Tris-HCl, PH8.0.
Shipping The product is shipped at -20°C temperature. Upon receipt, store it immediately at the temperature listed below.
Storage Reconstituted protein solution can be stored at 4-7°C for 1-2 months, stable at < -20°C for 2-3 years.
Reconstitution Reconstituted protein solution can be diluted with distilled PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Purity Purity≥85% as determined by reducing SDS-PAGE.
Endotoxin /
Bioactive Unknown. (Ban’t be used in biological activity experiments such as cell culture, treatment and diagnosis.)
规格 10ug 50ug 100ug 1mg

重组RNase抑制剂Recombinant RNase Inhibitor ver.2.0酶试剂盒Takara Clontech

上海金畔生物科技有限公司代理Takara酶试剂盒全线产品,欢迎访问官网了解更多产品信息和订购。

重组RNase抑制剂Recombinant RNase Inhibitor ver.2.0
品牌 Code No. 产品名称 包装量 价格(元) 说明书 数量
Takara 2315A Recombinant RNase Inhibitor ver.2.0 5,000 U ¥792
¥673
重组RNase抑制剂Recombinant RNase Inhibitor ver.2.0 重组RNase抑制剂Recombinant RNase Inhibitor ver.2.0 重组RNase抑制剂Recombinant RNase Inhibitor ver.2.0
Takara 2315B (A × 5) Recombinant RNase Inhibitor ver.2.0 5,000 Ux5 ¥3,762
¥3198
重组RNase抑制剂Recombinant RNase Inhibitor ver.2.0 重组RNase抑制剂Recombinant RNase Inhibitor ver.2.0 重组RNase抑制剂Recombinant RNase Inhibitor ver.2.0

*红字为促销价格,促销时间: 2024年3月1日-2024年4月30日

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Recombinant RNase Inhibitor ver.2.0
 
■ 制品说明:
Recombinant RNase Inhibitor ver.2.0是猪肝RNA酶抑制剂重组蛋白的升级制品。本制品特性与猪肝或者人胎盘来源的RNase Inhibitor相似,但由于半胱氨酸易氧化变性,因此在制品升级时,通过对半胱氨酸残基引入突变,提高了本制品的稳定性。
与RNase A形成 1:1复合体,抑制RNase活性。该反应是可逆的,通过尿素及巯基类试剂能够解离复合体,使RNase复性而抑制剂不可逆失活。本制品属蛋白质性质,与其它竞争性抑制剂(核酸类、无机磷酸类)不同,可以很容易地通过苯酚处理将其从反应体系中除去。此外,本制品不抑制RNase H活性。
本制品可以直接加入到RNA完整性十分重要的各种反应液中,如体外转录、RT-PCR等。
 
■ 用途:
1. 体外转录/翻译(反应量1 U/μl)
2. 用无细胞提取物进行体外转录/翻译(反应量20 U/μl)
3. RT-PCR (反应量0.5 U/μl)
4. cDNA合成(反应量0.5 U/μl)
5. 多核糖体分离(反应量 1 U/μl)
*括号显示了每个反应液中RNase抑制剂浓度的示例
 
■ 制品内容:(Code No. 2315A)
Recombinant RNase Inhibitor ver.2.0(40 U/μl) 5,000 U
 
■ 保存:
-20℃
 
■ 浓度:
40 U/μl
 
■ 酶贮存溶液:
20 mM HEPES-KOH, pH7.5
50 mM KCl
5 mM DTT
50% Glycerol
 
■ 起源:
E. coli carrying the plasmid containing the gene for ribonuclease inhibitor from porcine liver.
 
■ 特性:
1. 分子量:约52 kDa。
2. 最适pH:虽然在较宽的pH范围内可以起到抑制作用,但最大为pH7-8。
 
■ 活性定义:
抑制 5 ng RNase A 活性的 50%所需要的酶量定义为 1 个活性单位 (U)。(抑制活性通过抑制 RNase A 水解 2’,3’ Cyclic -CMP 的能力确定)
 
■ 使用注意:
1. 不要剧烈混匀蛋白质。
2. 可以在反应中加入一些DTT以确保活性,只要其浓度不干扰反应(例如,1 mM)。
 
 

页面更新:2024-01-31 16:18:03

Rabbit anti-CD47 Recombinant Monoclonal Antibody(295-33)

Rabbit anti-CD47 Recombinant Monoclonal Antibody(295-33)别名宿主反应种属应用免疫原形式浓度纯化方法类型克隆号储存/保存方法存储溶液背景说明细胞定位UniProt

概述
别名
Leukocyte surface antigen CD47; IAP; MER6
宿主
Rabbit
反应种属
Human, Mouse, Rat
应用
WB: 1:1000, IHC-P: 1:2000-1:4000
免疫原
Synthetic peptide
性能
形式
Liquid
浓度
0.5 mg/ml
纯化方法
Protein A affinity column
类型
Monoclonal Antibody
克隆号
295-33
储存/保存方法
Store at -20℃ for one year.
存储溶液
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
靶标
背景说明
The transmembrane protein, CD47, is recognized as an important innate immune checkpoint, and CD47-targeted drugs have been in development with the aim of inhibiting the interaction between CD47 and the regulatory glycoprotein SIRPα, for antitumor immunotherapy. Further, CD47 mediates other essential functions such as cell proliferation, caspase-independent cell death (CICD), angiogenesis and other integrin-activation-dependent cell phenotypic responses when bound to thrombospondin-1 (TSP-1) or other ligands. Mounting strategies that target CD47 have been developed in pre-clinical and clinical trials, including antibodies, small molecules, siRNAs, and peptides, and some of them have shown great promise in cancer treatment [PMID: 35931392].
细胞定位
Cell Membrane
UniProt
Q08722

实验结果图

Rabbit anti-CD47 Recombinant Monoclonal Antibody(295-33)

WB result of CD47 Rabbit mAb Primary antibody: CD47 Rabbit mAb at 1/1000 dilution Lane 1: HepG2 whole cell unboiled RIPA lysate 20ug Lane 2: Jurkat whole cell unboiled RIPA lysate 20ug Lane 3: OVCAR-3 whole cell unboiled RIPA lysate 20ug Negative control: HepG2 whole cell lysate Secondary antibody: #JP20040 at 1/10000 dilution Predicted MW: 35kDa Observed MW: 40~60kDa Exposure time: 20s

Rabbit anti-CD47 Recombinant Monoclonal Antibody(295-33)

IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-CD47 antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Rabbit anti-CD47 Recombinant Monoclonal Antibody(295-33)

IHC shows positive staining in paraffin-embedded human breast cancer. Anti-CD47 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Rabbit anti-CD47 Recombinant Monoclonal Antibody(295-33)

IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-CD47 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Rabbit anti-CD47 Recombinant Monoclonal Antibody(295-33)

IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-CD47 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Rabbit anti-CD47 Recombinant Monoclonal Antibody(295-33)

IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-CD47 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Rabbit anti-MPO Recombinant Monoclonal Antibody(224-72)

Rabbit anti-MPO Recombinant Monoclonal Antibody(224-72)别名宿主反应种属应用免疫原形式浓度纯化方法类型克隆号储存/保存方法存储溶液背景说明UniProt

概述
别名
Myeloperoxidase
宿主
Rabbit
反应种属
Human
应用
Sandwich ELISA
免疫原
Recombinant protein
性能
形式
Liquid
浓度
2 mg/ml
纯化方法
Protein A affinity column
类型
Monoclonal Antibody
克隆号
224-72
储存/保存方法
Store at 2 to 8℃ for one year.
存储溶液
PBS, pH 7.4, 0.03% Proclin 300
靶标
背景说明
Myeloperoxidase (MPO) is a peroxidase enzyme that in humans is encoded by the MPO gene on chromosome 17. MPO is most abundantly expressed in neutrophil granulocytes (a subtype of white blood cells), and produces hypohalous acids to carry out their antimicrobial activity, including hypochlorous acid, the sodium salt of which is the chemical in bleach. It is a lysosomal protein stored in azurophilic granules of the neutrophil and released into the extracellular space during degranulation. Neutrophil myeloperoxidase has a heme pigment, which causes its green color in secretions rich in neutrophils, such as mucus and sputum. The green color contributed to its outdated name verdoperoxidase. Recent studies have reported an association between elevated myeloperoxidase levels and the severity of coronary artery disease. And Heslop et al. reported that elevated MPO levels more than doubled the risk for cardiovascular mortality over a 13-year period. It has also been suggested that myeloperoxidase plays a significant role in the development of the atherosclerotic lesion and rendering plaques unstable.
UniProt
P05164

实验结果图

Rabbit anti-MPO Recombinant Monoclonal Antibody(224-72)

配对表格

Recombinant human ASMTL protein

Recombinant human ASMTL protein

货号:
P03720

品牌:
Jinpan

产品简介
基因名 ASMTL
英文全称 acetylserotonin O-methyltransferase-like
别称 ASMTLX;ASMTLY;ASTML
中文名称 乙酰5-羟色胺O-甲基转移酶
Uniprot O95671
GeneID 8623
交叉反应(推测) Human
氨基酸大小 38kDa
表达氨基酸 272-621aa
Background Nucleoside triphosphate pyrophosphatase that hydrolyzes dTTP and UTP.Can also hydrolyze CTP and the modified nucleotides pseudo-UTP, 5-methyl-UTP (m5UTP) and 5-methyl-CTP (m5CTP).Has weak activity with dCTP, 8-oxo-GTP and N4-methyl-dCTP.May have a dual role in cell division arrest and in preventing the incorporation of modified nucleotides into cellular nucleic acids.In addition, the presence of the putative catalytic domain of S-adenosyl-L-methionine binding in the C-terminal region argues for a methyltransferase activity.
Application Immunology research
Derived from E.coli
Purification NI-NTA affinity purification
Formulation Lyophilized from a 0.2 μm filtered solution of Tris-HCl, PH8.0.
Shipping The product is shipped at -20°C temperature. Upon receipt, store it immediately at the temperature listed below.
Storage Reconstituted protein solution can be stored at 4-7°C for 1-2 months, stable at < -20°C for 2-3 years.
Reconstitution Reconstituted protein solution can be diluted with distilled PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Purity Purity≥85% as determined by reducing SDS-PAGE.
Endotoxin /
Bioactive Unknown. (Ban’t be used in biological activity experiments such as cell culture, treatment and diagnosis.)
规格 10ug 50ug 100ug 1mg

Recombinant human CC2D1B protein

Recombinant human CC2D1B protein

货号:
P03725

品牌:
Jinpan

产品简介
基因名 CC2D1B
英文全称 coiled-coil and C2 domain containing 1B
别称 KIAA1836
中文名称 含coiled-coil 和C2结构域蛋白1B
Uniprot Q5T0F9
GeneID 200014
交叉反应(推测) Human Mouse Rat
氨基酸大小 17kDa
表达氨基酸 1-159aa
Background Transcription factor that binds specifically to the DRE (dual repressor element) and represses HTR1A gene transcription in neuronal cells.
Application Immunology research
Derived from E.coli
Purification NI-NTA affinity purification
Formulation Lyophilized from a 0.2 μm filtered solution of Tris-HCl, PH8.0.
Shipping The product is shipped at -20°C temperature. Upon receipt, store it immediately at the temperature listed below.
Storage Reconstituted protein solution can be stored at 4-7°C for 1-2 months, stable at < -20°C for 2-3 years.
Reconstitution Reconstituted protein solution can be diluted with distilled PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Purity Purity≥85% as determined by reducing SDS-PAGE.
Endotoxin /
Bioactive Unknown. (Ban’t be used in biological activity experiments such as cell culture, treatment and diagnosis.)
规格 10ug 50ug 100ug 1mg

Rabbit anti-MPO Recombinant Monoclonal Antibody(224-19)

Rabbit anti-MPO Recombinant Monoclonal Antibody(224-19)别名宿主反应种属应用免疫原形式浓度纯化方法类型克隆号储存/保存方法存储溶液背景说明UniProt

概述
别名
Myeloperoxidase
宿主
Rabbit
反应种属
Human
应用
Sandwich ELISA
免疫原
Recombinant protein
性能
形式
Liquid
浓度
2 mg/ml
纯化方法
Protein A affinity column
类型
Monoclonal Antibody
克隆号
224-19
储存/保存方法
Store at 2 to 8℃ for one year.
存储溶液
PBS, pH 7.4, 0.03% Proclin 300
靶标
背景说明
Myeloperoxidase (MPO) is a peroxidase enzyme that in humans is encoded by the MPO gene on chromosome 17. MPO is most abundantly expressed in neutrophil granulocytes (a subtype of white blood cells), and produces hypohalous acids to carry out their antimicrobial activity, including hypochlorous acid, the sodium salt of which is the chemical in bleach. It is a lysosomal protein stored in azurophilic granules of the neutrophil and released into the extracellular space during degranulation. Neutrophil myeloperoxidase has a heme pigment, which causes its green color in secretions rich in neutrophils, such as mucus and sputum. The green color contributed to its outdated name verdoperoxidase. Recent studies have reported an association between elevated myeloperoxidase levels and the severity of coronary artery disease. And Heslop et al. reported that elevated MPO levels more than doubled the risk for cardiovascular mortality over a 13-year period. It has also been suggested that myeloperoxidase plays a significant role in the development of the atherosclerotic lesion and rendering plaques unstable.
UniProt
P05164

实验结果图

Rabbit anti-MPO Recombinant Monoclonal Antibody(224-19)

配对表格

Recombinant human BDH2 protein

Recombinant human BDH2 protein

货号:
P03723

品牌:
Jinpan

产品简介
基因名 BDH2
英文全称 3-hydroxybutyrate dehydrogenase, type 2
别称 DHRS61 SDR15C1
中文名称 2型3-羟丁酸脱氢酶
Uniprot Q9BUT1
GeneID 56898
交叉反应(推测) Human Mouse Rat (Bovine Zebrafish)
氨基酸大小 27kDa
表达氨基酸 1-245aa
Background Dehydrogenase that mediates the formation of 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin and associates with LCN2, thereby playing a key role in iron assimilation and homeostasis.Plays a role in susceptibility to bacterial infection by providing an assimilable source of iron that is exploited by pathogenic bacteria.Also acts as a 3-hydroxybutyrate dehydrogenase.
Application Immunology research
Derived from E.coli
Purification NI-NTA affinity purification
Formulation Lyophilized from a 0.2 μm filtered solution of Tris-HCl, PH8.0.
Shipping The product is shipped at -20°C temperature. Upon receipt, store it immediately at the temperature listed below.
Storage Reconstituted protein solution can be stored at 4-7°C for 1-2 months, stable at < -20°C for 2-3 years.
Reconstitution Reconstituted protein solution can be diluted with distilled PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Purity Purity≥85% as determined by reducing SDS-PAGE.
Endotoxin /
Bioactive Unknown. (Ban’t be used in biological activity experiments such as cell culture, treatment and diagnosis.)
规格 10ug 50ug 100ug 1mg

Rabbit anti-PINP Recombinant Monoclonal Antibody(237-78)

Rabbit anti-PINP Recombinant Monoclonal Antibody(237-78)别名宿主反应种属应用免疫原形式浓度纯化方法类型克隆号储存/保存方法存储溶液背景说明UniProt

概述
别名
N-terminal propeptide of type 1 precollagen
宿主
Rabbit
反应种属
Human
应用
Sandwich ELISA
免疫原
Recombinant protein
性能
形式
Liquid
浓度
2 mg/ml
纯化方法
Protein A affinity column
类型
Monoclonal Antibody
克隆号
237-78
储存/保存方法
Store at 2 to 8℃ for one year.
存储溶液
PBS, pH 7.4, 0.03% Proclin 300
靶标
背景说明
N-terminal propeptide of type 1 precollagen (P1NP) is one of the markers of bone formation. Osteoblasts contain a large amount of typeⅠprecollagen. During bone formation, typeⅠprocollagen is secreted outside the cell and cleaved by specific protease to form typeⅠcollagen N-terminal procollagen of type 1 precollagen (P1NP) and C-terminal procollagen of type 1 precollagen, PICP) and typeⅠcollagen 3 fragments. Type I collagen is assembled in osteoid where inorganic minerals (calcium and phosphorus) are deposited to form hydroxyapatite (osteoid mineralization). P1NP and PICP enter the blood and urine as metabolites, so the detection of P1NP and PICP can reflect the level of bone formation. As the research evidence of P1NP reflecting bone formation is richer than PICP, P1NP is a better marker of bone formation. P1NP has good specificity, little influence on circadian rhythm and feeding, and is stable at room temperature. At present, P1NP is generally recommended as the preferred bone formation marker.
UniProt
P02452

实验结果图

Rabbit anti-PINP Recombinant Monoclonal Antibody(237-78)

配对表格