Adipogen热销蛋白–Progranulin (human) (rec.) (untagged)

Adipogen热销蛋白–Progranulin (human) (rec.) (untagged)

Progranulin (PGRN)是一种广泛表达的多能生长因子,通过激活控制细胞周期进展和细胞运动的信号级联反应,在发育、创伤修复和炎症等过程中发挥作用。它在中枢神经系统中的功能值得关注,因为在额颞退行性变(FTLD)病例中发现了PGRN基因突变。此外,PGRN也与肿瘤发生有关。新的前沿出版物表明,PGRN不仅可能是FTLD的生物标志物,也可能是其他疾病如阿尔茨海默病(AD)的生物标志物。此外,PGRN被描述为TNF受体的一种新的配体和用于潜在治疗如关节炎等炎症性疾病。

产品详情

别名:Proepithelin; PEPI; PC Cell-derived Growth Factor

来源:HEK 293 cells

浓度:1 mg/ml after reconstitution.

分子量:~90kDa (glycosylated protein by SDS-PAGE)

纯度:≥98% (SDS-PAGE)

生物活性:Activates phospho-ERK1/2 in neuronal mouse P19 cells. Regulates food intake and body weight (see reference 1).

内毒素水平:<0.01EU/µg purified protein (LAL test; Lonza).

产品检测实例:

右图是使用本产品对神经元分化的小鼠P19细胞中磷酸-ERK1/2和非磷酸-ERK1/2的影响进行分析。将未分化的小鼠P19细胞在含有10%胎牛血清的最低必需培养基(MEM)中用1μM维甲酸(RA)诱导分化3~4天,形成聚集体。然后在无RA的情况下将聚集体放置在平板上培养3~4天。分别在37°C下在0、5、10、30、60、120分钟内通过向神经元分化的小鼠P19细胞中加入重组蛋白(500ng/ml)进行反应,该细胞在血清饥饿下维持24小时。在泳道1、2、3、4、5和6分别在0、5、10、30、60和120分钟内用本产品进行处理,显影成像结果(GAPDH用作上样对照)。

订购信息:

产品名称

货号

规格

Progranulin (human) (rec.) (untagged)

AG-40A-0188Y-C050

50 ug

更多详情请联系Adipogen中国代理商上海金畔生物

Adipogen热销产品–CD40L (human) (multimeric) (rec.)

Adipogen热销产品–CD40L (human) (multimeric) (rec.)

产品描述

MultimericCD40L™ 是一种高活性构建体,其中两个三聚体CD40配体通过ACRP30的胶原结构域人工连接。该构建体非常有效地模拟体内CD40L的天然膜辅助聚集,它是激活CD40的最有效的替代物,不需要增强剂。MultimericCD40L™ 已显示以剂量、CD40和时间依赖性方式抑制明矾诱导的IL-1β释放和胱天蛋白酶-1活化,而不影响BMDM活力。它还有效地抑制了由NLRP3激活剂触发的炎性体功能。半胱天冬酶-1非依赖性炎症介质的分泌已被证明是不变的甚至增强的。

产品详情

别名:MultimericCD40L™; ACRP30headless:CD40L; ACRP30headless:CD154; ACRP30headless:TNFSF5; ADIPOQ-CD40L 

来源:CHO cells

特异性:Binds to human CD40.

浓度:0.1mg/ml after reconstitution.

分子量:~35-40kDa (SDS-PAGE)

纯度:≥95% (SDS-PAGE)

生物活性:Induces B cells activation (as demonstrated by dose-dependent upregulation of CD86) (ED50: <1ng>

内毒素水平:<0.01EU/μg purified protein (LAL test, Lonza).

产品订购

产品名称

货号

规格

CD40L (human) (multimeric) (rec.)

AG-40B-0010-C010

10 ug

该产品自上市以来有多篇文献引用,品质有保证,以下是最新发表的文献引用:

1.Detection of alloreactive T cells from cryopreserved human peripheral blood mononuclear cells: N. Tanimine, et al.; J. Immunol. Meth. 491, 112987 (2021)

2.Sensitive identification of neoantigens and cognate TCRs in human solid tumors: M. Arnaud, et al.; Nat. Biotechnol. 40, 656 (2022) 

详情请咨询Adipogen全国代理-上海金畔生物科技 

ISG15 (human) (rec.) (Rhodamine 110)产品介绍

ISG15 (human) (rec.) (Rhodamine 110)产品介绍

品牌:Adipogen

货号:SBB-PS0002-C050

品名:ISG15 (human) (rec.) (Rhodamine 110)

产品信息–

产品类型:蛋白

来源:E.coil。

序列:Human ISG15 (aa1-157) (Accession Nr. P05161) conjugated at the C-terminus to a quenched Rhodamine 110 dye.

种属反应:Human

应用:基于蛋白质的底物。典型的工作浓度范围为50-500nm。缀合物的水解导致荧光可观察到485nm的激发,并在535nm处发射。

偶联物:罗丹明

分子量:大约18kDa

纯度:≥97%(LCMS)

成分:液体。在50mM MES pH6.0中,100mM氯化钠,10%甘油。

产品介绍:

ISG15(干扰素刺激的基因,15kDa)是一种泛素样改性剂,其通过激活钻井平台-1信号传导,刺激NK细胞增殖,抑制病毒萌发并作用为IFNγ诱导细胞因子来启动先天性免疫应答。ISG15含有两个串联泛素同源域,并且与α-泛素抗体的交叉反应。ISG15对底物蛋白的缀合通过通过E1(UBE1L),E2(UBCH8 / UBE2L6)和E3(尚未发现的级联等钙蛋白发生。通常通过UBP43(USP18)进行欺诈,但有几种病毒蛋白酶能够水解ISG15缀合物以逃避免疫应答。这些包括含有氨纶刚果出血热的OTU的蛋白酶骨病毒和SARS冠状病毒的木瓜蛋白酶样蛋白酶(PLPRO)。

详情请咨询Adipogen代理商-上海金畔生物科技

Mega CD40LTM (soluble)(h)(rec.) ALX-522-110

Mega CD40LTM (soluble)(h)(rec.) ALX-522-110

MegaCD40LTM (soluble)(h)(rec.)     ALX-522-110

产品特征:

高纯度,内毒素含量低。

活性高

批次间差异小

粉末状态增加稳定性和活力

分子量: ~38kDa

浓度: 重悬后0.1mg/ml

状态: 冻干粉(含PBS

纯度:≥90% (SDS-PAGE)

内毒素含量:≤0.01EU/µg


文献引用:

A human monoclonal antibody targeting the conserved staphylococcal antigen IsaA protects mice against Staphylococcus aureus bacteremia: S. van den Berg, et al.; Int. J. Med. Microbiol. 305, 55 (2015), Application(s): ELISA, PCR of human blood samples, Abstract;

CD40L Induces Functional Tunneling Nanotube Networks Exclusively in Dendritic Cells Programmed by Mediators of Type 1 Immunity: C. Zaccard, et al.; J. Immunol. 194, 1047 (2015), Application(s): Activation of mature human dendritic cells,Abstract;

A GPBAR1 (TGR5) small molecule agonist shows specific inhibitory effects on myeloid cell activation in vitro and reduces experimental autoimmune encephalitis (EAE) in vivo: N. Lewis, et al.; PLoS One 9, e100883 (2014), Application(s): Assay of human PBMC, AbstractFull Text

Development of a novel anti-canine CD20 monoclonal antibody with diagnostic and therapeutic potential: D. Ito, et al.; Leuk. Lymphoma 56, 219 (2014), Application(s): Analysis of canine B-cell lymphoma cells, Abstract;

Exosomes Derived from Burkitt’s Lymphoma Cell Lines Induce Proliferation, Differentiation, and Class-Switch Recombination in B Cells: C. Gutzeit, et al.; J. Immunol. 192, 5852 (2014), Application(s): PCR, Assay of human B cells, AbstractFull Text

Preclinical evaluation of the novel, orally bioavailable Selective Inhibitor of Nuclear Export (SINE) KPT-335 in spontaneous canine cancer: results of a phase I study: C. London, et al.; PLoS One 9, e87585 (2014), Application(s): Analysis of canine tumor cells by WB, FLOW, AbstractFull Text

Thyrotropin receptor and CD40 mediate interleukin-8 expression in fibrocytes: implications for thyroid-associated ophthalmopathy (an American Ophthalmological Society thesis).: R. Douglas, et al.; Trans. Am. Ophthalmol. Soc. 12, 26 (2014), Application(s): In vitro analysis of human fibrocytes, AbstractFull Text

AMPKα1 deficiency amplifies proinflammatory myeloid APC activity and CD40 signaling: K.C. Carroll, et al.; J. Leukoc. Biol.94, 1113 (2013), Abstract;

CD40 ligand is necessary and suffi cient to support primary diff use large B-cell lymphoma cells in culture: a tool for in vitro preclinical studies with primary B-cell malignancies: D. Ito, et al.; Leuk. Lymphoma 53, 1390 (2012), Application(s): Flow cytometry of human B cells, AbstractFull Text

CD40 ligand is necessary and sufficient to support primary diffuse large B-cell lymphoma cells in culture: a tool for in vitro preclinical studies with primary B-cell malignancies: D. Ito, et al.; Leuk. Lymphoma 53, 1390 (2012), Application(s): Cell Culture, Abstract;

Mega CD40LTM (soluble)(h)(rec.) ALX-522-110(1)

Mega CD40LTM (soluble)(h)(rec.) ALX-522-110(1)

MegaCD40LTM (soluble)(h)(rec.)     ALX-522-110

产品特征:

高纯度,内毒素含量低。

活性高

批次间差异小

粉末状态增加稳定性和活力

分子量: ~38kDa

浓度: 重悬后0.1mg/ml

状态: 冻干粉(含PBS

纯度:≥90% (SDS-PAGE)

内毒素含量:≤0.01EU/µg


文献引用:

A human monoclonal antibody targeting the conserved staphylococcal antigen IsaA protects mice against Staphylococcus aureus bacteremia: S. van den Berg, et al.; Int. J. Med. Microbiol. 305, 55 (2015), Application(s): ELISA, PCR of human blood samples, Abstract;

CD40L Induces Functional Tunneling Nanotube Networks Exclusively in Dendritic Cells Programmed by Mediators of Type 1 Immunity: C. Zaccard, et al.; J. Immunol. 194, 1047 (2015), Application(s): Activation of mature human dendritic cells,Abstract;

A GPBAR1 (TGR5) small molecule agonist shows specific inhibitory effects on myeloid cell activation in vitro and reduces experimental autoimmune encephalitis (EAE) in vivo: N. Lewis, et al.; PLoS One 9, e100883 (2014), Application(s): Assay of human PBMC, AbstractFull Text

Development of a novel anti-canine CD20 monoclonal antibody with diagnostic and therapeutic potential: D. Ito, et al.; Leuk. Lymphoma 56, 219 (2014), Application(s): Analysis of canine B-cell lymphoma cells, Abstract;

Exosomes Derived from Burkitt’s Lymphoma Cell Lines Induce Proliferation, Differentiation, and Class-Switch Recombination in B Cells: C. Gutzeit, et al.; J. Immunol. 192, 5852 (2014), Application(s): PCR, Assay of human B cells, AbstractFull Text

Preclinical evaluation of the novel, orally bioavailable Selective Inhibitor of Nuclear Export (SINE) KPT-335 in spontaneous canine cancer: results of a phase I study: C. London, et al.; PLoS One 9, e87585 (2014), Application(s): Analysis of canine tumor cells by WB, FLOW, AbstractFull Text

Thyrotropin receptor and CD40 mediate interleukin-8 expression in fibrocytes: implications for thyroid-associated ophthalmopathy (an American Ophthalmological Society thesis).: R. Douglas, et al.; Trans. Am. Ophthalmol. Soc. 12, 26 (2014), Application(s): In vitro analysis of human fibrocytes, AbstractFull Text

AMPKα1 deficiency amplifies proinflammatory myeloid APC activity and CD40 signaling: K.C. Carroll, et al.; J. Leukoc. Biol.94, 1113 (2013), Abstract;

CD40 ligand is necessary and suffi cient to support primary diff use large B-cell lymphoma cells in culture: a tool for in vitro preclinical studies with primary B-cell malignancies: D. Ito, et al.; Leuk. Lymphoma 53, 1390 (2012), Application(s): Flow cytometry of human B cells, AbstractFull Text

CD40 ligand is necessary and sufficient to support primary diffuse large B-cell lymphoma cells in culture: a tool for in vitro preclinical studies with primary B-cell malignancies: D. Ito, et al.; Leuk. Lymphoma 53, 1390 (2012), Application(s): Cell Culture, Abstract;

DNER (extracellular domain) (human) (rec.)产品介绍

DNER (extracellular domain) (human) (rec.)产品介绍

品牌:Adipogen

货号:AG-40A-0137Y-C010

品名:DNER (extracellular domain) (human) (rec.)

产品信息:

产品类型:蛋白

来源:HEK293 cells

种属反应:Human

分子量:120-140kDa左右(SDS-PAGE)

纯度:≥90% (SDS-PAGE)

内毒素水平:<0.1EU/μg纯化蛋白(LAL试验;Lonza)。

浓度:重组后0.1 mg /ml

成分:冻干。包含PBS + 0.02% CHAPS。

稳定性:在-20°C下保存至少6个月。

产品介绍:

DNER是Notch1通路的激活物。它介导星形细胞形成过程中神经元-胶质细胞的相互作用。

详情请咨询Adipogen中国代理商-上海金畔生物技术公司