标签归档：pbasi

shRNA表达载体pBAsi vector series酶试剂盒Takara Clontech

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shRNA表达载体pBAsi vector series
品牌 Code No. 产品名称 包装量 价格（元） 说明书 数量
Takara 3220 pBAsi-hH1 DNA(库存销售完毕即终卖) 20 μg ￥1,994 shRNA表达载体pBAsi vector series shRNA表达载体pBAsi vector series shRNA表达载体pBAsi vector series
Takara 3221 pBAsi-hU6 DNA(库存销售完毕即终卖) 20 μg ￥1,994 shRNA表达载体pBAsi vector series shRNA表达载体pBAsi vector series shRNA表达载体pBAsi vector series
Takara 3222 pBAsi-mU6 DNA(库存销售完毕即终卖) 20 μg ￥1,994 shRNA表达载体pBAsi vector series shRNA表达载体pBAsi vector series shRNA表达载体pBAsi vector series
Takara 3223 pBAsi-hH1 Pur DNA 20 μg ￥1,994 shRNA表达载体pBAsi vector series shRNA表达载体pBAsi vector series shRNA表达载体pBAsi vector series
Takara 3224 pBAsi-hU6 Pur DNA 20 μg ￥1,994 shRNA表达载体pBAsi vector series shRNA表达载体pBAsi vector series shRNA表达载体pBAsi vector series
Takara 3225 pBAsi-mU6 Pur DNA 20 μg ￥1,994 shRNA表达载体pBAsi vector series shRNA表达载体pBAsi vector series shRNA表达载体pBAsi vector series
Takara 3227 pBAsi-hU6 Neo DNA(库存销售完毕即终卖) 20 μg ￥1,994 shRNA表达载体pBAsi vector series shRNA表达载体pBAsi vector series shRNA表达载体pBAsi vector series
Takara 3228 pBAsi-mU6 Neo DNA(库存销售完毕即终卖) 20 μg ￥1,994 shRNA表达载体pBAsi vector series shRNA表达载体pBAsi vector series shRNA表达载体pBAsi vector series
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shRNA表达载体pBAsi vector series   shRNA表达载体pBAsi vector series   shRNA表达载体pBAsi vector series → shRNA表达载体pBAsi vector series
  →   →  
shRNA表达载体pBAsi vector series   shRNA表达载体pBAsi vector series   shRNA表达载体pBAsi vector series
 
 
□ 贮存溶液  
     Tris-HCl (pH 8.0) 10 mM
     EDTA 1 mM
 
■ 制品说明
pBAsi vector series是利用RNA polymerase III (pol III) 系列的promoter表达siRNA的质粒载体。且该质粒搭载了新霉素抗性基因及嘌 呤霉素抗性基因。可用于导入细胞的筛选。通过在pol III系列的promoter的下游插入表达harpin型RNA的合成DNA序列可制备siRNA 表达质粒。将得到的质粒转入细胞中，即可用于RNAi实验 (参照下图) 。
shRNA表达载体pBAsi vector series
 
此外，可以将promoter+hairpin型RNA序列切下来，重组到Adeno病毒载体上。由于Adeno病毒载体具有很高的感染效率和广泛的感染域， 可通过in vivo或者in vitro实验导入基因。关于重组Adeno病毒的制备请使用Adenovirus Dual Expression Kit（Code No. 6170） 。
 
■ 保存
-20℃。
 
■ pBAsi载体图谱
shRNA表达载体pBAsi vector series
 
shRNA表达载体pBAsi vector series
 
shRNA表达载体pBAsi vector series
 

页面更新：2024-04-01 16:26:25