Cell（MB-231 and MDA-MB-468 cells），0.75~24 mmol/mL podophyllotoxin，48h
The effect of podophyllotoxin(purity:≥ 98%, Jinpan, China) on cell viability was determined by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide assay, for which MDA MB-231 and MDA-MB-468 cells were cultured in 96-well plates. The density of MDA-MB-231 cells was 1.2 × 104 per well, while that of MDA-MB-468 cells was 1.5 × 104 cells per well. Before adding the drug, the cells were cultured in a serumfree medium for 6h and treated with varying concentrations of podophyllotoxin (0.75, 1.5, 3, 6, 12, and 24 mmol/ml) for 48 h. After that, each well was incubated with 15 ml of MTT for 4 h, and 150-ml dimethyl sulfoxide was added after incubation. After shaking, the microplate reader was used to detect the absorbance at 570 nm.
来源文献：Zhang W, Liu C, Li J, Liu R, Zhuang J, Feng F, Yao Y, Sun C. Target Analysis and Mechanism of Podophyllotoxin in the Treatment of Triple-Negative Breast Cancer. Front Pharmacol. 2020 Aug 7;11:1211. doi: 10.3389/fphar.2020.01211. PMID: 32848800; PMCID: PMC7427588.
