Epicypher热销产品——Nucleosome, Recombinant Human, H3K4me3 dNuc, Biotinylated

发表于2024年6月21日由上海金畔生物科技有限公司

EpiCypher是一家为表观遗传学和染色质生物学研究提供高质量试剂和工具的专业制造商。EpiCypher生产的重组单核小体（H3K4me3）由 147 个DNA碱基对缠绕在组蛋白的八聚体核心（H2A、H2B、H3.2 和 H4 各两个）上构成，是染色质的基本单位。Lowary和Widom[1]鉴定的147bp 601序列对组蛋白八聚体具有高亲和力，可用于核小体组装。H3K4me3 dNuc 在组蛋白H3.2的第4位含有三甲基赖氨酸。H3K4me3在110位处有一个Cys到Ala的取代。该DNA含有一个5’biotin-TEG基团。EpiCypher生产的重组单核小体适用于多种应用，包括用作酶分析、高通量筛选和抑制剂测试、染色质结合研究、蛋白质-蛋白质相互作用分析、结构研究和效应蛋白结合实验中的底物。

[1] Lowary and Widom J. Mol. Biol. (1998). PMID: 9514715

产品详情

宿主来源: Human

表达系统: E. coli & synthetic DNA

标签: Biotinylated

保存温度: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws

产品形式: 0.977 mg/mL mononucleosome in 51.2 µL 10 mM Tris HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol (27.3 µg protein, 50 µg DNA + protein)

验证数据

	Figure 1: Western blot data Western Analysis of H3K4me3 dNuc. Top Panel: Unmodified (EpiCypher 16-0006; Lane 1) and H3K4me3 (Lane 2) nucleosomes were probed with an anti-H3K4me3 antibody and analyzed via ECL readout. Only the H3K4me3 sample produced a detectable signal. Bottom Panel: Detail from Coomassie stained gel showing unmodified (Lane 1) and H3K4me3 (Lane 2) nucleosomes.

Figure 2: Mass spec data Synthetic H3K4me3 histone analyzed by high resolution mass spectrometry. Expected mass = 15,267.77 Da. Determined mass = 15,266.15 Da.
	Figure 3: Protein gel data Coomassie stained SDS-PAGE gel of proteins in H3K4me3 dNuc (1 µg) demonstrates the purity of histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H3K4me3 and H4) are indicated.
	Figure 4: DNA gel data H3K4me3 dNuc resolved via native PAGE gel and stained with ethidium bromide to visualize DNA. Lane 1: Free DNA (EpiCypher 18-0005; 100 ng). Lane 2: Intact H3K4me3 nucleosomes (400 ng).

订购详情

货号	产品名称	规格
16-0316	Nucleosome, Recombinant Human, H3K4me3 dNuc, Biotinylated	50 µg
16-0316-20	Nucleosome, Recombinant Human, H3K4me3 dNuc, Biotinylated	20 µg

如需了解更多详细信息或相关产品，请联系EpiCypher中国代理商-上海金畔生物

Epicypher热销产品——EpiDyne-FRET Nucleosome Remodeling Assay Substrate

发表于2024年6月21日由上海金畔生物科技有限公司

Epicypher热销产品——EpiDyne-FRET Nucleosome Remodeling Assay Substrate

当与酶活性重塑复合物如SMARCA4、SMARCA2和ACF（如EpiCypher货号15-1014、15-1015、15-1013）配对时，该产品提供核小体重塑的高通量荧光读数。EpiDyne®-FRET单核小体由在大肠杆菌中表达的重组人组蛋白（组蛋白H2A-Cy5、H2B、H3.2和H4各两种；accession numbers:：H2A-P4908；H2B-O60814；H3.2-Q71DI3；H4-P62805）组装而成，该组蛋白由212个DNA碱基对缠绕，并携带5’Cy3、Widom 601核小体定位元件和重塑时暴露的DpnII限制性内切酶基序（GATC）[1]。H2A在残基120位处具有Thr-Cys取代，其中Cy5是共轭的。H3.2在残基110位处具有Cys-to-Ala取代。

[1] Lowary & Widom J Mol. Biol. (1998) PMID: 9514715.

产品详情

保存温度:Stable for six (6) months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.

运输温度:Dry ice.

产品形式:Purified recombinant mononucleosomes, (21.8 µg protein weight, 50 µg DNA+protein) in 44.2 µL of 10 mM Tris-HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. MW = 240,650 Da. Molarity = 4.7 µM.

验证数据

	Figure 1: DNA Gel Data EpiDyne-FRET substrate resolved via native PAGE gel and stained with ethidium bromide to visualize DNA. Lane 1: Free DNA (100 ng). Lane 2: Intact EpiDyne-FRET nucleosomes (400 ng).
	Figure 2: Protein Gel Data Coomassie stained PAGE gel of proteins in EpiDyne-FRET substrate (1 µg) demonstrates the purity of histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A-Cy5, H2B, H3.2, and H4) are indicated.

Figure 3: Nucleosome Remodeling Data ACF1/ATP-dependent nucleosome remodeling reaction. EpiDyne-FRET nucleosomes (15 nM) were incubated with ACF1 chromatin remodeler (EpiCypher 15-1013; indicated concentrations) in the presence or absence of 1 mM ATP. Upon the addition of ATP, reactions were immediately read in an Envision Multilabelplate reader (PerkinElmer). Data are presented as the mean of the Cy3/Cy5 ratio (N=2).

订购详情

货号	产品名称	规格
16-4201	EpiDyne-FRET Nucleosome Remodeling Assay Substrate	50 µg

如需了解更多详细信息或相关产品，请联系EpiCypher中国代理商-上海金畔生物

Epicypher热销产品——Nucleosome, Recombinant Human, H2BK120ub1 dNuc, Biotinylated

发表于2024年6月21日由上海金畔生物科技有限公司

Epicypher热销产品——Nucleosome, Recombinant Human, H2BK120ub1 dNuc, Biotinylated

EpiCypher是一家为表观遗传学和染色质生物学研究提供高质量试剂和工具的专业制造商。EpiCypher生产的在E. coli中表达的重组人单核小体(组蛋白H2A、H2B、H3和H4各2个;accession numbers:H2A-P04908;H2B-O60814;H3.1-P68431;H4-P62805)由601位处的147个DNA碱基对缠绕在组蛋白上构成。组蛋白H2B(由专门的半合成方法产生)在120位处含有泛素赖氨酸。核小体是染色质的基本单位，Lowary和Widom鉴定的601序列是一个含有147碱基对的序列，对组蛋白八聚体具有高亲和力，可用于核小体组装，并含有5’biotin-TEG基团。适合用作酶筛选分析或效应蛋白结合实验的底物。

[1] Lowary and Widom J. Mol. Biol. (1998). PMID: 9514715

产品详情

标签: Biotinylated

保存温度:Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.

产品形式: H2BK120ub dNuc (14.4 µg protein weight, 25 µg DNA + protein) in 22.5 µL 10 mM Tris HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. Molarity = 5.12 μM. MW = 216,985.9 Da.

验证数据

	Figure 1: Western blot data Western Analysis of Nucleosome, Recombinant Human, H2BK120ub. Top Panel: Unmodified H3 (Lane 1) and H2BK120ub containing nucleosomes (Lane 2) were probed with an anti-H2BK120ub antibody and analyzed via ECL readout. Only the H2BK120ub sample produced a detectable signal. Bottom Panel: Detail from Coomassie stained gel showing unmodified nucleosomes (Lane 1) and H2BK120ub nucleosomes (Lane 2).
	Figure 2: Mass spec data H2BK120ub protein analyzed by high resolution mass spectrometry. Expected mass = 22,321.8 Da. Determined mass = 22,320.3 Da.
	Figure 3: Protein gel data Coomassie stained PAGE gel of proteins in Nucleosome, Recombinant Human, H2BK120ub (1 μg) to demonstrate the purity of the histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2BK120ub, H3.1, and H4) are indicated.
	Figure 4: DNA gel data Nucleosome, Recombinant Human, H2BK120ub dNucs resolved via native PAGE and stained with ethidium bromide to visualize DNA. Lane 1: Free DNA (100 ng). Lane 2: Intact H2BK120ub nucleosome (400 ng).