将6至8周龄的雄性Balb / c nu / nu小鼠皮下注射到两侧，将5×105 LNCaP 104-R1细胞悬浮于0.5mL Matrigel中，并皮下注射到无胸腺小鼠中以形成肿瘤。 14周后，平均肿瘤体积超过150mm 3。然后将小鼠分成对照组和咖啡酸苯乙酯（CAPE）处理组。对照组含有6只小鼠和8只肿瘤，而咖啡酸苯乙酯处理组含有6只小鼠和9只肿瘤。从癌细胞注射后第14周开始通过管饲法施用咖啡酸苯乙酯（芝麻油中10mg / kg /天）或载体（芝麻油）。使用卡尺每周测量携带104-R1异种移植物的小鼠的肿瘤体积和体重，并使用公式体积=长度×宽度×高度×0.52 [1]计算体积。
[1]. Lin HP, et al. Caffeic acid phenethyl ester induced cell cycle arrest and growth inhibition in androgen-independent prostate cancer cells via regulation of Skp2, p53, p21Cip1 and p27Kip1. Oncotarget. 2015 Mar 30;6(9):6684-707
[1]. Liang Y, et al. Berbamine, a novel nuclear factor kappaB inhibitor, inhibits growth and induces apoptosis in human myeloma cells. Acta Pharmacol Sin. 2009 Dec;30(12):1659-65. [2]. Meng Z, et al. Berbamine inhibits the growth of liver cancer cells and cancer-initiating cells by targeting Ca2+/calmodulin-dependent protein kinase II. Mol Cancer Ther. 2013 Oct;12(10):2067-77.
将用JSH-23孵育的巨噬细胞RAW 264.7用1μg/ mL LPS和/或样品处理24小时。将细胞用1μg/ mL DAPI在37℃下染色30分钟，然后使用荧光显微镜在300-500nm激发下进行分析。具有包含明显浓缩的染色质的细胞核或具有片段化细胞核的细胞被评分为细胞凋亡指数。
数据来源文献
[1]. Shin, H.M., et al. Inhibitory action of novel aromatic diamine compound on lipopolysaccharide-induced nuclear translocation of NF-kappaB without affecting IkappaB degradation. FEBS Lett, 2004. 571(1-3): p. 50-4. [2]. Arias-Salvatierra, D., et al. Role of nitric oxide produced by iNOS through NF-kappaB pathway in migration of cerebellar granule neurons induced by Lipopolysaccharide. Cell Signal, 2011. 23(2): p. 425-35. [3]. Kasparkova, J., et al. Different affinity of nuclear factor-kappa B proteins to DNA modified by antitumor cisplatin and its clinically ineffective trans isomer. FEBS J, 2014. 281(5): p. 1393-408. [4]. Kumar, A., et al. JSH-23 targets nuclear factor-kappa B and reverses various deficits in experimental diabetic neuropathy: effect on neuroinflammation and antioxidant defence. Diabetes Obes Metab, 2011. 13(8): p. 750-8
