产品名称

InVivoPlus anti-mouse Ly6G

产品货号

BP0075-1

产品规格

5/25/50/100mg

反应种属

Mouse

克隆号

1A8

同种型

Rat IgG2a, κ

免疫原

EL4J cells transfected with Ly6G

实验应用

in vivo neutrophil depletion

in vivo MDSC depletion

Immunofluorescence

Immunohistochemistry (paraffin)

Immunohistochemistry (frozen)

Flow cytometry

产品形式

PBS, pH 7.0,Contains no stabilizers or preservatives

纯度

>95%, Determined by SDS-PAGE

聚合

<5%, Determined by SEC

无菌处理

0.2 µm filtration

纯化方式

Protein G

RRID

AB_1107721

分子量

150 kDa

小鼠病原检测

Ectromelia/Mousepox Virus: Negative

Hantavirus: Negative

K Virus: Negative

Lactate Dehydrogenase-Elevating Virus: Negative

Lymphocytic Choriomeningitis virus: Negative

Mouse Adenovirus: Negative

Mouse Cytomegalovirus: Negative

Mouse Hepatitis Virus: Negative

Mouse Minute Virus: Negative

Mouse Norovirus: Negative

Mouse Parvovirus: Negative

Mouse Rotavirus: Negative

Mycoplasma Pulmonis: Negative

Pneumonia Virus of Mice: Negative

Polyoma Virus: Negative

Reovirus Screen: Negative

Sendai Virus: Negative

Theiler’s Murine Encephalomyelitis: Negative

保存条件

抗体原液保存在4°C，不能冷冻保存。

推荐同型对照

InVivoPlus rat IgG2a isotype control, anti-trinitrophenol（货号BP0089）

推荐抗体稀释液

InVivoPure pH 7.0 Dilution Buffer（货号IP0070）

应用

文章

体内中性粒细胞耗竭

(in vivo neutrophil depletion)

1. Davis, R. W. t., et al. (2018). ‘Luminol Chemiluminescence Reports Photodynamic Therapy-Generated Neutrophil Activity In Vivo and Serves as a Biomarker of Therapeutic Efficacy’ Photochem Photobiol .

2. Moynihan, K. D., et al. (2016). ‘Eradication of large established tumors in mice by combination immunotherapy that engages innate and adaptive immune responses’ Nat Med. doi : 10.1038/nm.4200.

3. Conde, P., et al. (2015). ‘DC-SIGN(+) Macrophages Control the Induction of Transplantation Tolerance’ Immunity 42(6): 1143-1158.

4. Griseri, T., et al. (2015). ‘Granulocyte Macrophage Colony-Stimulating Factor-Activated Eosinophils Promote Interleukin-23 Driven Chronic Colitis’ Immunity 43(1): 187-199.

5. Yamada, D. H., et al. (2015). ‘Suppression of Fcgamma-receptor-mediated antibody effector function during persistent viral infection’ Immunity 42(2): 379-390.

6. Ellis, G. T., et al. (2015). ‘TRAIL+ monocytes and monocyte-related cells cause lung damage and thereby increase susceptibility to influenza-Streptococcus pneumoniae coinfection’ EMBO Rep 16(9): 1203-1218.

7. Deshmukh, H. S., et al. (2014). ‘The microbiota regulates neutrophil homeostasis and host resistance to Escherichia coli K1 sepsis in neonatal mice’ Nat Med 20(5): 524-530.

体内中性粒细胞耗竭、流式细胞术、免疫组化石蜡切片(in vivo neutrophil depletion, Flow Cytometry, Immunohistochemistry (paraffin))

Coffelt, S. B., et al. (2015). ‘IL-17-producing gammadelta T cells and neutrophils conspire to promote breast cancer metastasis’ Nature 522(7556): 345-348.

体内中性粒细胞耗竭、流式细胞术、免疫组化石蜡切片、免疫组化冰冻切片(in vivo neutrophil depletion, Flow Cytometry, Immunohistochemistry (paraffin), Immunohistochemistry (frozen))

Finisguerra, V., et al. (2015). ‘MET is required for the recruitment of anti-tumoural neutrophils’ Nature 522(7556): 349-353.

体内中性粒细胞耗竭、流式细胞术(in vivo neutrophil depletion, Flow Cytometry)

1.Moser, E. K., et al. (2014). ‘Late engagement of CD86 after influenza virus clearance promotes recovery in a FoxP3+ regulatory T cell dependent manner’ PLoS Pathog 10(8): e1004315.

2. Chen, K. W., et al. (2014). ‘The neutrophil NLRC4 inflammasome selectively promotes IL-1beta maturation without pyroptosis during acute Salmonella challenge’ Cell Rep 8(2): 570-582.

3. Garraud, K., et al. (2012). ‘Differential role of the interleukin-17 axis and neutrophils in resolution of inhalational anthrax’ Infect Immun 80(1): 131-142.

4. Lee, W. B., et al. (2012). ‘Neutrophils Promote Mycobacterial Trehalose Dimycolate-Induced Lung Inflammation via the Mincle Pathway’ PLoS Pathog 8(4): e1002614.

体内骨髓来源抑制细胞耗竭(in vivo MDSC depletion)

Deng, L., et al. (2014). ‘Irradiation and anti-PD-L1 treatment synergistically promote antitumor immunity in mice’ J Clin Invest 124(2): 687-695.

体内中性粒细胞耗竭、免疫荧光(in vivo neutrophil depletion,Immunofluorescence)

Edelson, B. T., et al. (2011). ‘CD8alpha(+) dendritic cells are an obligate cellular entry point for productive infection by Listeria monocytogenes’ Immunity 35(2): 236-248.