寡核苷酸阵列比较基因组杂交标记试剂盒 CGH labeling kit for oligo arrays

寡核苷酸阵列比较基因组杂交标记试剂盒 CGH labeling kit for oligo arrays

CGH labeling kit for oligo arrays   ENZ-42671

 寡核苷酸阵列比较基因组杂交标记试剂盒 

产品特点:

无需扩增或降低复杂度,能够实现整个基因组DNA分析;

能够为各种复杂的异质样品提供良好的动态分析检测范围

QC基准经高分辨度分析验证

货号

产品名称

规格

品牌

ENZ-42671-K010

CGH labeling kit for oligo arrays 

20 reactions

ENZO(美国)

ENZ-42671-K010

CGH labeling kit for oligo arrays 

200 reactions

ENZO(美国)


文献引用:

First contiguous gene deletion causing biotinidase deficiency: The enzyme deficiency in three Sri Lankan children: D.N. Senanayake, et al.; Mol. Gen. Met. Rep. 2, 81 (2015), Application(s): DNA Labeling,

A new direction for prenatal chromosome microarray testing: software-targeting for detection of clinically significant chromosome imbalance without equivocal findings: J.W. Ahn, et al.; Peer J. 2, e354 (2014), Application(s): Labeling of DNA extracted from chorionic villi and amniotic fluid samples, Abstract; Full Text

Analysis of MYB oncogene in transformed adenoid cystic carcinomas reveals distinct pathways of tumor progression: A.F. Costa, et al.; Lab. Invest. 94, 692 (2014), Abstract;

Mutation screening and array comparative genomic hybridization using a 180K oligonucleotide array in VACTERL association: J. Winberg, et al.; PLoS One 9, 85313 (2014), Abstract; Full Text

Phenotypic features in patients with 15q11.2(BP1-BP2) deletion: Further delineation of an emerging syndrome: M. Cafferkey, et. al.; Am. J. Med. Genet. A. Epub ahead of print, (2014), Abstract;

An improved method to extract DNA from 1ml of uncultured amniotic fluid from patients at less than 16 weeks’ gestation: A.L. Mosca-Boidron, et al.; PLoS One 8, e59956 (2013), Abstract; Full Text

Chromosome 20p11 gains are associated with liver-specific metastasis in patients with colorectal cancer: L.J. Mekenkamp, et al.; Gut 62, 94 (2013), Abstract;

DNA copy number profiling reveals extensive genomic loss in hereditary BRCA1 and BRCA2 ovarian carcinomas: M.M. Kamieniak, et al.; Br. J. Cancer 108, 1732 (2013), Abstract; Full Text

Focal aberrations indicate EYA2 and hsa‐miR‐375 as oncogene and tumor suppressor in cervical carcinogenesis: M. Bierkens, et al.; Genes Chromosomes Cancer 52, 56 (2013), Abstract;

Germline copy number variation of genes involved in chromatin remodelling in families suggestive of Li-Fraumeni syndrome with brain tumours: J. Aury-Landas, et al.; Eur. J. Hum. Genet. 21, 1369 (2013), Abstract;

Intellectual disability and hemizygous GPD2 mutation: D.Q. Barge-Schaapveld, et al.; Am. J. Med. Genet. A. 161A, 1044 (2013), Abstract;

Male-biased autosomal effect of 16p13.11 copy number variation in neurodevelopmental disorders: M. Tropeano, et al.; PLoS One 8, e61365 (2013), Application(s): Blood samples from patients with neurodevelopmental disorders, Abstract; Full Text

寡核苷酸阵列比较基因组杂交标记试剂盒 CGH labeling kit for oligo arrays(1)

寡核苷酸阵列比较基因组杂交标记试剂盒 CGH labeling kit for oligo arrays(1)

CGH labeling kit for oligo arrays   ENZ-42671

 寡核苷酸阵列比较基因组杂交标记试剂盒 

产品特点:

无需扩增或降低复杂度,能够实现整个基因组DNA分析;

能够为各种复杂的异质样品提供良好的动态分析检测范围

QC基准经高分辨度分析验证

货号

产品名称

规格

品牌

ENZ-42671-K010

CGH labeling kit for oligo arrays 

20 reactions

ENZO(美国)

ENZ-42671-K010

CGH labeling kit for oligo arrays 

200 reactions

ENZO(美国)


文献引用:

First contiguous gene deletion causing biotinidase deficiency: The enzyme deficiency in three Sri Lankan children: D.N. Senanayake, et al.; Mol. Gen. Met. Rep. 2, 81 (2015), Application(s): DNA Labeling,

A new direction for prenatal chromosome microarray testing: software-targeting for detection of clinically significant chromosome imbalance without equivocal findings: J.W. Ahn, et al.; Peer J. 2, e354 (2014), Application(s): Labeling of DNA extracted from chorionic villi and amniotic fluid samples, Abstract; Full Text

Analysis of MYB oncogene in transformed adenoid cystic carcinomas reveals distinct pathways of tumor progression: A.F. Costa, et al.; Lab. Invest. 94, 692 (2014), Abstract;

Mutation screening and array comparative genomic hybridization using a 180K oligonucleotide array in VACTERL association: J. Winberg, et al.; PLoS One 9, 85313 (2014), Abstract; Full Text

Phenotypic features in patients with 15q11.2(BP1-BP2) deletion: Further delineation of an emerging syndrome: M. Cafferkey, et. al.; Am. J. Med. Genet. A. Epub ahead of print, (2014), Abstract;

An improved method to extract DNA from 1ml of uncultured amniotic fluid from patients at less than 16 weeks’ gestation: A.L. Mosca-Boidron, et al.; PLoS One 8, e59956 (2013), Abstract; Full Text

Chromosome 20p11 gains are associated with liver-specific metastasis in patients with colorectal cancer: L.J. Mekenkamp, et al.; Gut 62, 94 (2013), Abstract;

DNA copy number profiling reveals extensive genomic loss in hereditary BRCA1 and BRCA2 ovarian carcinomas: M.M. Kamieniak, et al.; Br. J. Cancer 108, 1732 (2013), Abstract; Full Text

Focal aberrations indicate EYA2 and hsa‐miR‐375 as oncogene and tumor suppressor in cervical carcinogenesis: M. Bierkens, et al.; Genes Chromosomes Cancer 52, 56 (2013), Abstract;

Germline copy number variation of genes involved in chromatin remodelling in families suggestive of Li-Fraumeni syndrome with brain tumours: J. Aury-Landas, et al.; Eur. J. Hum. Genet. 21, 1369 (2013), Abstract;

Intellectual disability and hemizygous GPD2 mutation: D.Q. Barge-Schaapveld, et al.; Am. J. Med. Genet. A. 161A, 1044 (2013), Abstract;

Male-biased autosomal effect of 16p13.11 copy number variation in neurodevelopmental disorders: M. Tropeano, et al.; PLoS One 8, e61365 (2013), Application(s): Blood samples from patients with neurodevelopmental disorders, Abstract; Full Text

APC Antibody Labeling Kits(APC抗体标记试剂盒) 货号: S601122S/S601122M/S601122L 规格: 50μg/100μg/500μg

APC Antibody Labeling Kits(APC抗体标记试剂盒)

产品货号: S601122S/S601122M/S601122L

产品规格: 50μg/100μg/500μg

目录价(元):1250/1680/3980

大包装询价


产品概述:

产品内容

组分

S601122S (50 μg)

S601122M (100 μg)

S601122L (500 μg)

A: Modified APC

1 vial

1 vial

1 vial

B: Linking Reagent

1 vial

1 vial

1 vial

C: Storage Buffer

100 μL

100 μL

100 μL

D: Ultrafiltration vial (MWCO=10KDa)

2 each

2 each

2 each

 
储存条件
-20℃避光保存,有效期见外包装。

产品应用
APC抗体标记可以兼容NaN3、低浓度的甘油、Tris和甘氨酸。试剂盒里提供的微型超滤离心管可以在标记前快速去除不兼容的小分子抗体稳定剂。
我们还提供酶标记和荧光标记的 Super-n-stain®试剂盒(请访问www.uelandy.com)。


说明书:

APC Antibody Labeling Kits(APC抗体标记试剂盒) 货号:               S601122S/S601122M/S601122L  规格:               50μg/100μg/500μg UE-S601122S/S601122M/S601122L    
MSDS:

MSDS S601122 APC Antibody Labeling Kits
使用本产品的文献:

参考文献
1.Paraoxonase 2 decreases renal reactive oxygen species production, lowers blood pressure, and mediates dopamine D2 receptor-induced inhibition of NADPH oxidase
应用方向:polyclonal rabbit anti-PON2 antibody/  polyclonal rabbit anti-D2R antibody 

2.The Q loops of the human multidrug resistance transporter ABCB1 are necessary to couple drug binding to the ATP catalytic cycle
应用方向:Primary antibody 4E3

3.Increased Metabolite Levels of Glycolysis and Pentose Phosphate Pathway in Rabbit Atherosclerotic Arteries and Hypoxic Macrophage
应用方向:anti-Ki-67 antibody/anti-macrophage antibody / anti-SMC antibody

4.Mucosal immunoglobulins at respiratory surfaces mark an ancient association that predates the emergence of tetrapods
应用方向:rabbit anti-trout IgT / mouse anti-trout IgM / rabbit anti-Ich

5.Data ofrationalprocessoptimization for the production of a full IgG and its Fab fragment from hybridoma cells
应用方向:IgG

6.Adenoviral L4 33K forms ring-like oligomers and stimulates ATPase activity of IVa2: implications in viral genome packaging
应用方向:polyclonal anti-IVa2 and anti-33K antibodies

7.Principal cell activity induces spine relocation of adult-born interneurons in the olfactory bulb
应用方向:Anti-synaptophysin antibody

8.Enhancing proteasome-inhibitory activity and specificity of bortezomib by CD38 targeted nanoparticles in multiple myeloma
应用方向:anti-CD38 chitosan NPs

DNA探针标记试剂盒BcaBEST™ Labeling Kit酶试剂盒Takara Clontech

上海金畔生物科技有限公司代理Takara酶试剂盒全线产品,欢迎访问官网了解更多产品信息和订购。

DNA探针标记试剂盒BcaBEST Labeling Kit
品牌 Code No. 产品名称 包装量 价格(元) 说明书 数量
Takara 6046 BcaBEST Labeling Kit 40 Rxns ¥722 DNA探针标记试剂盒BcaBEST™ Labeling Kit DNA探针标记试剂盒BcaBEST™ Labeling Kit DNA探针标记试剂盒BcaBEST™ Labeling Kit
收藏产品 加入购物车
 
■ 制品内容 (40 次量) DNA探针标记试剂盒BcaBEST™ Labeling Kit
Random Primer (9 mer) 80 μl
10 × Buffer 100 μl
dNTP Mixture 100 μl
(dGTP,dATP,dTTP) (各0.2 mM)  
BcaBEST DNA Polymerase (2 U/μl) 40 μl
Control DNA (λ-Hind Ⅲ Fragment) (25 ng/μl) 10 μl
   
 
■ 制品说明
BcaBEST Labeling Kit是一种在DNA上标记[α-32P]、[3H] dCTP后,可在短时间内合成DNA 探针的试剂盒。本试剂盒的设计以A.P.Feinberg和B.Vogelstion 法为基础并对其进行了改良,操作十分简便,合成后的探针比活性高。目前我们经常使用的Klenow fragment 存在反应时间长、对GC 含量高的DNA 模板或具有高级结构的DNA 摄入效率低等缺点。Bacillus caldotenax 来源的BcaBEST DNA Polymerase是一种耐热性的、高延伸性的DNA 聚合酶。BcaBEST DNA Polymerase 和随机引物 (9 mer) 在50℃~ 55℃条件下反应10 分钟,即可对具有高级结构的DNA 模板进行标记。
BcaBEST DNA Polymerase 具有优良的延伸性,与Klenow fragment相比,可以合成更长的探针,同时因BcaBEST DNA Polymerase不具备exonuclease活性,反应时间长也不会导致摄入效率下降。
 
表1. 与Random Primer DNA Labeling Kit Ver.2比较
     BcaBEST Labeling Kit  Random Primer DNA Labeling Kit Ver.2
反应时间 10分钟 10分钟
探针的比放射活性 109 dpm/μg 109 dpm/μg
模板DNA 不受高级结构、GC含量的影响  高级结构或GC含量高时可导致摄入效率下降
注:两种试剂盒使用300 bp以下的DNA模板时,可导致摄入效率下降。
 
利用随机引物进行DNA标记的原理
DNA探针标记试剂盒BcaBEST™ Labeling Kit
 
 

页面更新:2024-01-31 13:47:31

杂交用DNA探针制备试剂盒Random Primer DNA Labeling Kit Ver.2酶试剂盒Takara Clontech

上海金畔生物科技有限公司代理Takara酶试剂盒全线产品,欢迎访问官网了解更多产品信息和订购。

杂交用DNA探针制备试剂盒Random Primer DNA Labeling Kit Ver.2
品牌 Code No. 产品名称 包装量 价格(元) 说明书 数量
Takara 6045 Random Primer DNA Labeling Kit Ver.2 30 Rxns ¥667 杂交用DNA探针制备试剂盒Random Primer DNA Labeling Kit Ver.2 杂交用DNA探针制备试剂盒Random Primer DNA Labeling Kit Ver.2 杂交用DNA探针制备试剂盒Random Primer DNA Labeling Kit Ver.2
收藏产品 加入购物车
 
■ 制品内容 (30 次量)
Random Primer (9 mer) 60 μl
10 × Buffer 75 μl
dNTP Mixture (dGTP,dATP,dTTP各0.2 mM) 75 μl
Exo-free Klenow Fragment (2 U/μl) 30 μl
Control DNA solution (λ-Hind III digest 25 ng/μl) 10 μl
 
■ 制品说明
本试剂盒利用 [α32P]或[3H]dCTP标记DNA,制备具有高比活性的杂交用DNA探针。本试剂盒的设计以Feinberg和Vogelstein法为基础加以了改进,利用9个碱基的随机寡聚核苷酸引物以及E. coli DNA Polymerase I 的 Exonuclease-free Klenow Fragment (无3′ →5′ 外切酶活性) ,在短时间内 (2-3分钟)即能得到比活性为1×109 dpm/μg的探针。它克服了表1中切口平移法的许多缺点。
 
■ 原 理
利用杂交法检定具有特异序列的DNA时,必须使用高比放射活性的DNA探针。Feinberg和Vogelstein发表的随机引物标记法从少量DNA (10-20 ng) 制得的探针具有很高的比活性,并且可以直接在低熔点琼脂糖凝胶中标记DNA片段。原理如图1所示。首先通过热变性使模板DNA变为单链DNA,然后使随机引物与单链DNA退火,再利用Klenow Fragment合成互补链形成标记和未标记的核苷酸。
表1
     切口平移法  随机引物法
反应时间 延长反应时间会降低标记效率。     短时间内即可得到高比活性的探针。即使反应时间延长也不受影响。
探针比活性 108 dpm/μg     109 dpm/μg
模板纯度 琼脂糖等抑制反应     琼脂糖对反应没有影响
反应后纯化 需要除去未反应的dNTP     不需要除去未反应的dNTP
模板量要求 ≥1 μg     ≥25 ng
以上对比数据的依据:以50 μCi的[α-32P]dCTP(370 MBq/ml)标记λ DNA-Hind III,以此为模板进行多重对照实验。
 
杂交用DNA探针制备试剂盒Random Primer DNA Labeling Kit Ver.2
图1 Random Primer Labeling原理
 
 
 

页面更新:2024-02-29 08:29:58

PE Antibody Labeling Kits(PE抗体标记试剂盒) 货号: S601123S/S601123M/S601123L 规格: 50μg/100μg/500μg

PE Antibody Labeling Kits(PE抗体标记试剂盒)

产品货号: S601123S/S601123M/S601123L

产品规格: 50μg/100μg/500μg

目录价(元):1250/1680/3980

大包装询价


产品概述:

产品内容

组分

S601123S (50 μg)

S601123M (100 μg)

S601123L (500 μg)

A: Modified PE

1 vial

1 vial

1 vial

B: Linking Reagent

1 vial

1 vial

1 vial

C: Storage Buffer

100 μL

100 μL

100 μL

D: Ultrafiltration vial (MWCO=10KDa

2 each

2 each

2 each


储存条件
-20℃避光保存,有效期见外包装。

产品应用
PE抗体标记可以兼容NaN3、低浓度的甘油、Tris和甘氨酸。试剂盒里提供的微型超滤离心管可以在标记前快速去除不兼容的小分子抗体稳定剂。
我们还提供酶标记和荧光标记的 Super-n-stain®试剂盒(请访问www.uelandy.com)。


说明书:

PE Antibody Labeling Kits(PE抗体标记试剂盒) 货号:               S601123S/S601123M/S601123L  规格:               50μg/100μg/500μg UE-S601123S/S601123M/S601123L    
MSDS:

MSDS S601123 PE Antibody Labeling Kits
常见问题解答:


使用本产品的文献:

参考文献
1.Paraoxonase 2 decreases renal reactive oxygen species production, lowers blood pressure, and mediates dopamine D2 receptor-induced inhibition of NADPH oxidase
应用方向:polyclonal rabbit anti-PON2 antibody/  polyclonal rabbit anti-D2R antibody 

2.The Q loops of the human multidrug resistance transporter ABCB1 are necessary to couple drug binding to the ATP catalytic cycle
应用方向:Primary antibody 4E3

3.Increased Metabolite Levels of Glycolysis and Pentose Phosphate Pathway in Rabbit Atherosclerotic Arteries and Hypoxic Macrophage
应用方向:anti-Ki-67 antibody/anti-macrophage antibody / anti-SMC antibody

4.Mucosal immunoglobulins at respiratory surfaces mark an ancient association that predates the emergence of tetrapods
应用方向:rabbit anti-trout IgT / mouse anti-trout IgM / rabbit anti-Ich

5.Data ofrationalprocessoptimization for the production of a full IgG and its Fab fragment from hybridoma cells
应用方向:IgG

6.Adenoviral L4 33K forms ring-like oligomers and stimulates ATPase activity of IVa2: implications in viral genome packaging
应用方向:polyclonal anti-IVa2 and anti-33K antibodies

7.Principal cell activity induces spine relocation of adult-born interneurons in the olfactory bulb
应用方向:Anti-synaptophysin antibody

8.Enhancing proteasome-inhibitory activity and specificity of bortezomib by CD38 targeted nanoparticles in multiple myeloma
应用方向:anti-CD38 chitosan NPs

HRP Antibody Labeling Kits(HRP抗体标记试剂盒) 货号: H601124S/ H601124M/H601124L 规格: 50ug/100ug/500ug

HRP Antibody Labeling Kits(HRP抗体标记试剂盒)

产品货号: H601124S/ H601124M/H601124L

产品规格: 50ug/100ug/500ug

目录价(元):1250/1680/3980

大包装询价


产品概述:

产品内容

组分

H601124S

H601124M

H601124L

A: Modified HRP

50 μg

100 μg

500 μg

B: IgG修饰液

10 μL

20 μL

100 μL

C: 终止液干粉

25 μg

50 μg

250 μg

D: 5×抗体保存液)

20 μL

40 μL

200 μL

 
储存条件
-20℃避光保存,有效期见外包装。

产品应用
HRP(horseradish peroxidase,辣根过氧化物酶),是一种糖蛋白,广泛分布于植物中,分子量小,标记物容易穿透入细胞内部,比活性高,稳定,纯酶容易制备。常用于生化检测分析,免疫类Elisa试剂盒,是多种试剂盒显色体系的关键成分。
过氧化物酶标记抗体,是一类广泛用于免疫学、分子生物学及临床医学的各个分支学科中的特异、敏感和安全的免疫化学制剂。
我们提供了HRP抗体标记试剂盒以及标记好的HRP 羊抗鼠及羊抗兔二抗(请访问www.uelandy.com)。

注意事项
1. 标记体积大时,标记过程建议使用混匀仪。
2. 标记环境以0.01M pH7.4 PBS最佳,不应含有甘油、叠氮钠、氨基物(包括甘氨酸、Tris等),EDTA等物质。
3. 若含有以上物质,建议用PBS进行充分透析或者超滤管以除去以上物质。

HRP Antibody Labeling Kits(HRP抗体标记试剂盒) 货号:               H601124S/ H601124M/H601124L  规格:               50ug/100ug/500ug

V
1:IgG体积;V2:IgG修饰液体积;V3:终止液体积;V4:抗体保存液体积


说明书:

HRP Antibody Labeling Kits(HRP抗体标记试剂盒) 货号:               H601124S/ H601124M/H601124L  规格:               50ug/100ug/500ug UE-H601124S/H601124M/H601124L    
MSDS:

MSDS H601124 HRP Antibody Labeling Kits