BioXcell热销产品–InVivoPlus anti-mouse PD-1 (CD279)

发表于2024年6月13日由上海金畔生物科技有限公司

BioXcell热销产品–InVivoPlus anti-mouse PD-1 (CD279)

产品描述：

BioXcell InVivoPlus anti-mouse PD-1 RMP1-14单克隆抗体与小鼠PD-1（也称为CD279）反应。PD-1是一种50-55kDa的细胞表面受体，由Pdcd1基因编码，属于Ig超家族的CD28家族。PD-1在CD4和CD8胸腺细胞以及活化的T和B淋巴细胞和髓细胞上瞬时表达，在成功消除抗原后PD-1的表达下降。此外，在B细胞前阶段，Pdcd1 mRNA在发育中的B淋巴细胞中表达。PD-1的结构包括ITIM（基于免疫受体酪氨酸的抑制基序），这表明PD-1负调控TCR信号。PD-1通过结合B7家族的成员PD-L1和PD-L2发出信号。在配体结合后，PD-1信号传导抑制T细胞活化，导致增殖减少，细胞因子产生和T细胞死亡。此外，PD-1敲除动物表现出扩张型心肌病、脾肿大和外周耐受丧失，在小鼠的外周耐受性和预防自身免疫性疾病中发挥关键作用。诱导的PD-L1表达常见于许多肿瘤，包括鳞状细胞癌、结肠癌和乳腺癌。PD-L1过度表达会导致肿瘤细胞对CD8T细胞介导的裂解的抗性增加。在黑素瘤的小鼠模型中，可以通过用阻断PD-L1与其受体PD-1之间相互作用的抗体治疗来暂时抑制肿瘤生长。由于这些原因，目前正在探索抗PD-1介导的免疫疗法作为癌症治疗。与J43抗体一样，RMP1-14抗体已显示阻断小鼠PD-L1-Ig和小鼠PD-L2-Ig与PD-1的结合。

BioXcell热销产品--InVivoPlus anti-mouse PD-1 (CD279)

产品详情：

产品名称	InVivoPlus anti-mouse PD-1 (CD279)
产品货号	BP0146
产品规格	5/25/50/100mg
反应种属	Mouse
克隆号	RMP1-14
同种型	Rat IgG2a, κ
免疫原	Syrian Hamster BKH cells transfected with mouse PD-1 cDNA
实验应用	in vivo blocking of PD-1/PD-L signaling
产品形式	PBS, pH 7.0,Contains no stabilizers or preservatives
纯度	>95%, Determined by SDS-PAGE
聚合	<5%, Determined by SEC
无菌处理	0.2 µm filtration
纯化方式	Protein G
RRID	AB_10949053
分子量	150 kDa
小鼠病原检测	Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative
保存条件	抗体原液保存在4°C，不能冷冻保存。
推荐同型对照	InVivoPlus rat IgG2a isotype control, anti-trinitrophenol（货号BP0089）
推荐抗体稀释液	InVivoPure pH 7.0 Dilution Buffer（货号IP0070）

为什么选择InVivoPlus抗体？

InVivoPlus级别的产品内毒素含量更低，经过多种实验验证，更适合用于体内实验研究

BioXcell热销产品--InVivoPlus anti-mouse PD-1 (CD279)

该产品自上市已被多篇SCI文献引用，品质有保证，以下是部分已发表的文献引用：

应用

文章

体内PD-1/PD-L信号阻断

(in vivo blocking of PD-1/PD-L signaling)

1. Triplett, T. A., et al. (2018). ‘Reversal of indoleamine 2,3-dioxygenase-mediated cancer immune suppression by systemic kynurenine depletion with a therapeutic enzyme’ Nat Biotechnol 36(8): 758-764.

2. Grasselly, C., et al. (2018). ‘The Antitumor Activity of Combinations of Cytotoxic Chemotherapy and Immune Checkpoint Inhibitors Is Model-Dependent’ Front Immunol 9: 2100.

3. Moynihan, K. D., et al. (2016). ‘Eradication of large established tumors in mice by combination immunotherapy that engages innate and adaptive immune responses’ Nat Med. doi : 10.1038/nm.4200.

4. Ngiow, S. F., et al. (2015). ‘A Threshold Level of Intratumor CD8+ T-cell PD1 Expression Dictates Therapeutic Response to Anti-PD1’ Cancer Res 75(18): 3800-3811.

5. Evans, E. E., et al. (2015). ‘Antibody Blockade of Semaphorin 4D Promotes Immune Infiltration into Tumor and Enhances Response to Other Immunomodulatory Therapies’ Cancer Immunol Res 3(6): 689-701.

6. Zelenay, S., et al. (2015). ‘Cyclooxygenase-Dependent Tumor Growth through Evasion of Immunity’ Cell 162(6): 1257-1270.

7. Zander, R. A., et al. (2015). ‘PD-1 Co-inhibitory and OX40 Co-stimulatory Crosstalk Regulates Helper T Cell Differentiation and Anti-Plasmodium Humoral Immunity’ Cell Host Microbe 17(5): 628-641.

更多产品详情请咨询 BioXcell 中国代理——上海金畔生物

BioXcell热销产品–InVivoPlus anti-mouse CTLA-4 (CD152)

发表于2024年6月13日由上海金畔生物科技有限公司

BioXcell热销产品–InVivoPlus anti-mouse CTLA-4 (CD152)

BioXcell热销产品–InVivoPlus anti-mouse CTLA-4 (CD152)

产品描述：

BioXcell InVivoPlus anti-mouse CTLA-4 (CD152)克隆号9D9单克隆抗体能与小鼠CTLA-4(细胞毒性T淋巴细胞抗原-4)反应，CTLA-4也称为CD152。CTLA-4是一种33 kDa的细胞表面受体，由属于免疫球蛋白超家族CD28家族的Ctla4基因编码。CTLA-4在活化的T淋巴细胞和B淋巴细胞上表达。CTLA-4在结构上类似于T细胞共刺激蛋白CD28，两种分子都与B7家族成员B7-1 (CD80)和B7-2 (CD86)结合。在与配体结合时，CTLA-4负调节细胞介导的免疫反应。CTLA-4在诱导和/或维持免疫耐受、胸腺细胞发育和保护性免疫调节中起作用。CTLA-4在免疫下调中的关键作用已经在CTLA-4缺陷小鼠中得到证实，这些小鼠在3-5周龄时由于淋巴增生性疾病的发展而死亡。CTLA-4是通过免疫检查点阻断作为癌症治疗靶点的抑制性受体之一。

BioXcell热销产品--InVivoPlus anti-mouse CTLA-4 (CD152)

产品详情：

产品名称	InVivoPlus anti-mouse CTLA-4 (CD152)
产品货号	BP0164
产品规格	5/25/50/100mg
反应种属	Mouse
克隆号	9D9
同种型	Mouse IgG2b
实验应用	in vivo CTLA-4 neutralization Western blot
产品形式	PBS, pH 7.0,Contains no stabilizers or preservatives
纯度	>95%, Determined by SDS-PAGE
聚合	<5%, Determined by SEC
无菌处理	0.2 µm filtration
纯化方式	Protein A
RRID	AB_10949609
分子量	150 kDa
小鼠病原检测	Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative
保存条件	抗体原液保存在4°C，不能冷冻保存。
推荐同型对照	InVivoPlus mouse IgG2b isotype control, unknown specificity（货号BP0086）
推荐抗体稀释液	InVivoPure pH 7.0 Dilution Buffer（货号IP0070）

为什么选择InVivoPlus抗体？

InVivoPlus级别的产品内毒素含量更低，经过多种实验验证，更适合用于体内实验研究

BioXcell热销产品--InVivoPlus anti-mouse CTLA-4 (CD152)

该产品自上市已被多篇SCI文献引用，品质有保证，以下是部分已发表的文献引用：

应用

文章

体内CTLA-4中和

(in vivo CTLA-4 neutralization)

1. Dai, M., et al. (2015). ‘Curing mice with large tumors by locally delivering combinations of immunomodulatory antibodies’ Clin Cancer Res 21(5): 1127-1138.

2. Zippelius, A., et al. (2015). ‘Induced PD-L1 expression mediates acquired resistance to agonistic anti-CD40 treatment’ Cancer Immunol Res 3(3): 236-244.

3. Redmond, W. L., et al. (2014). ‘Combined targeting of costimulatory (OX40) and coinhibitory (CTLA-4) pathways elicits potent effector T cells capable of driving robust antitumor immunity’ Cancer Immunol Res 2(2): 142-153.

4. Condamine, T., et al. (2014). ‘ER stress regulates myeloid-derived suppressor cell fate through TRAIL-R-mediated apoptosis’ J Clin Invest 124(6): 2626-2639.

5. Muller, P., et al. (2014). ‘Microtubule-depolymerizing agents used in antibody-drug conjugates induce antitumor immunity by stimulation of dendritic cells’ Cancer Immunol Res 2(8): 741-755.

6. Bulliard, Y., et al. (2013). ‘Activating Fc gamma receptors contribute to the antitumor activities of immunoregulatory receptor-targeting antibodies’ J Exp Med 210(9): 1685-1693.

更多产品详情请咨询 BioXcell 中国代理——上海金畔生物

BioXcell热销产品–InVivoPlus rat IgG2a isotype control

发表于2024年6月13日由上海金畔生物科技有限公司

BioXcell热销产品–InVivoPlus rat IgG2a isotype control

BioXcell 热销产品–InVivoPlus rat IgG2a isotype control

产品描述：

BioXcell nVivoPlus rat IgG2a isotype control克隆号为2A3的同型对照抗体与三硝基苯酚反应。因为哺乳动物不表达三硝基苯酚，因此该抗体非常适合在大多数体内和体外应用中用作大鼠IgG2a抗体的同型对照。

BioXcell热销产品--InVivoPlus rat IgG2a isotype control

产品详情：

产品名称	InVivoPlus rat IgG2a isotype control
产品货号	BP0089
产品规格	5/25/50/100mg
克隆号	2A3
同种型	Rat IgG2a, κ
产品形式	PBS, pH 6.5,Contains no stabilizers or preservatives
纯度	>95%, Determined by SDS-PAGE
聚合	<5%, Determined by SEC
无菌处理	0.2 µm filtration
纯化方式	Protein G
RRID	AB_1107769
分子量	150 kDa
小鼠病原检测	Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative
保存条件	抗体原液保存在4°C，不能冷冻保存。
推荐同型对照	InVivoMAb rat IgG2a isotype control, anti-trinitrophenol（货号BE0089）
推荐抗体稀释液	InVivoPure pH 6.5 Dilution Buffer（货号IP0065）

为什么选择InVivoPlus抗体？

InVivoPlus级别的产品内毒素含量更低，经过多种实验验证，更适合用于体内实验研究

BioXcell热销产品--InVivoPlus rat IgG2a isotype control

该产品自上市已被多篇SCI文献引用，品质有保证，以下是部分已发表的文献引用：

文章

1.Bauche, D., et al. (2018). ‘LAG3(+) Regulatory T Cells Restrain Interleukin-23-Producing CX3CR1(+) Gut-Resident Macrophages during Group 3 Innate Lymphoid Cell-Driven Colitis’ Immunity 49(2): 342-352 e345.

2.Ngiow, S. F., et al. (2015). ‘A Threshold Level of Intratumor CD8+ T-cell PD1 Expression Dictates Therapeutic Response to Anti-PD1’ Cancer Res 75(18): 3800-3811.

3.Dai, M., et al. (2015). ‘Curing mice with large tumors by locally delivering combinations of immunomodulatory antibodies’ Clin Cancer Res 21(5): 1127-1138.

4.Kurtulus, S., et al. (2015). ‘TIGIT predominantly regulates the immune response via regulatory T cells’ J Clin Invest. doi : 10.1172/JCI81187.

5.Ellis, G. T., et al. (2015). ‘TRAIL+ monocytes and monocyte-related cells cause lung damage and thereby increase susceptibility to influenza-Streptococcus pneumoniae coinfection’ EMBO Rep 16(9): 1203-1218.

更多产品详情请咨询 BioXcell 中国代理——上海金畔生物

BioXcell热销产品推荐–InVivoPlus anti-mouse PD-L1 (B7-H1)

发表于2024年6月13日由上海金畔生物科技有限公司

BioXcell热销产品推荐–InVivoPlus anti-mouse PD-L1 (B7-H1)

BioXcell热销产品推荐–InVivoPlus anti-mouse PD-L1 (B7-H1)

产品描述：

BioXcell InVivoPlus anti-mouse PD-L1 10F.9G2单克隆抗体与小鼠PD-L1（也称为B7-H1或CD274）反应。PD-L1是属于Ig超家族的B7家族的I型跨膜蛋白，分子量为40kDa。PD-L1在T淋巴细胞、B淋巴细胞、NK细胞、树突状细胞以及IFNγ刺激的单核细胞、上皮细胞和内皮细胞上表达。PD-L1与CD4和CD8胸腺细胞以及活化的T和B淋巴细胞和骨髓细胞上的受体PD-1结合。PD-L1与PD-1的结合导致抑制TCR介导的T细胞增殖和细胞因子产生。PD-L1被认为在肿瘤免疫逃逸中起着重要作用。诱导的PD-L1表达在许多肿瘤中很常见，并导致肿瘤细胞对CD8 T细胞介导的裂解的抗性增加。在黑色素瘤的小鼠模型中，可以通过用阻断PD-L1和PD-1之间相互作用的抗体进行治疗，暂时抑制肿瘤生长。10F.9G2抗体已被证明可以阻断PD-L1和PD-1之间以及PD-L1和B7-1之间的相互作用（CD80）。

BioXcell热销产品推荐--InVivoPlus anti-mouse PD-L1 (B7-H1)

产品详情：

产品名称	InVivoPlus anti-mouse PD-L1 (B7-H1)
产品货号	BP0101
产品规格	5/25/50/100mg
反应种属	Mouse
克隆号	10F.9G2
同种型	Rat IgG2b, κ
免疫原	Mouse CD274
实验应用	in vivo PD-L1 blockade Immunofluorescence Immunohistochemistry (frozen) Flow cytometry Western blot
产品形式	PBS, pH 6.5,Contains no stabilizers or preservatives
纯度	>95%, Determined by SDS-PAGE
聚合	<5%, Determined by SEC
无菌处理	0.2 µm filtration
纯化方式	Protein G
RRID	AB_10949073
分子量	150 kDa
小鼠病原检测	Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative
保存条件	抗体原液保存在4°C，不能冷冻保存。
推荐同型对照	InVivoPlus rat IgG2b isotype control, anti-keyhole limpet hemocyanin（货号BP0090）
推荐抗体稀释液	InVivoPure pH 6.5 Dilution Buffer（货号IP0065）

为什么选择InVivoPlus抗体？

InVivoPlus级别的产品内毒素含量更低，经过多种实验验证，更适合用于体内实验研究

BioXcell热销产品推荐--InVivoPlus anti-mouse PD-L1 (B7-H1)

该产品自上市已被多篇SCI文献引用，品质有保证，以下是部分已发表的文献引用：

应用

文章

体内PD-L1阻断

(in vivo PD-L1 blockade)

1. Grasselly, C., et al. (2018). ‘The Antitumor Activity of Combinations of Cytotoxic Chemotherapy and Immune Checkpoint Inhibitors Is Model-Dependent’ Front Immunol 9: 2100.

2. Stathopoulou, C., et al. (2018). ‘PD-1 Inhibitory Receptor Downregulates Asparaginyl Endopeptidase and Maintains Foxp3 Transcription Factor Stability in Induced Regulatory T Cells’ Immunity 49(2): 247-263 e247.

3. Kim, J., et al. (2015). ‘Memory programming in CD8(+) T-cell differentiation is intrinsic and is not determined by CD4 help’ Nat Commun 6: 7994.

体内PD-L1阻断，流式细胞术

(in vivo PD-L1 blockade, Flow Cytometry)

1. Aloulou, M., et al. (2016). ‘Follicular regulatory T cells can be specific for the immunizing antigen and derive from naive T cells’ Nat Commun 7: 10579.

2. Ngiow, S. F., et al. (2015). ‘A Threshold Level of Intratumor CD8+ T-cell PD1 Expression Dictates Therapeutic Response to Anti-PD1’ Cancer Res 75(18): 3800-3811.

3. Rutigliano, J. A., et al. (2014). ‘Highly pathological influenza A virus infection is associated with augmented expression of PD-1 by functionally compromised virus-specific CD8+ T cells’ J Virol 88(3): 1636-1651.

体内PD-L1阻断，免疫荧光

(in vivo PD-L1 blockade, Immunofluorescence)

1.Willimsky, G., et al. (2013). ‘Virus-induced hepatocellular carcinomas cause antigen-specific local tolerance’ J Clin Invest 123(3): 1032-1043.

免疫组织化学（冷冻切片），免疫荧光

(Immunohistochemistry (frozen), Immunofluorescence)

1.Riella, L. V., et al. (2011). ‘Essential role of PDL1 expression on nonhematopoietic donor cells in acquired tolerance to vascularized cardiac allografts’ Am J Transplant 11(4): 832-840.

更多产品详情请咨询 BioXcell 中国代理——上海金畔生物

BioXcell热销产品–InVivoPlus anti-mouse CD8α

发表于2024年6月13日由上海金畔生物科技有限公司

BioXcell热销产品–InVivoPlus anti-mouse CD8α

BioXcell热销产品–InVivoPlus anti-mouse CD8α

产品描述：

BioXcell InVivoPlus anti-mouse CD8α克隆号YTS 169.4单克隆抗体能与小鼠CD8α反应。CD8抗原是一种跨膜糖蛋白，作为T细胞受体的共受体。与TCR一样，CD8与抗原呈递细胞(APC)展示的I类MHC分子结合。CD8主要在细胞毒性T细胞表面表达，但也可在胸腺细胞、自然杀伤细胞和一些树突状细胞亚群中发现。CD8最常见的存在形式是由一个CD8α和一个CD8β链组成的异二聚体，然而，它也可以是由两个CD8α链组成的同二聚体。CD8α和CD8β链均与免疫球蛋白可变轻链具有显著同源性。每个CD8链的分子量约为34 kDa。BioXcell InVivoPlus anti-mouse CD8α克隆号YTS 169.4抗体在体内使用时显示出depleting活性。

BioXcell热销产品--InVivoPlus anti-mouse CD8α

产品详情：

产品名称	InVivoPlus anti-mouse CD8α
产品货号	BP0117
产品规格	5/25/50/100mg
反应种属	Mouse
克隆号	YTS 169.4
同种型	Rat IgG2b, κ
免疫原	CBA mouse thymocytes
实验应用	in vivo CD8+ T cell depletion Western blot
产品形式	PBS, pH 7.0,Contains no stabilizers or preservatives
纯度	>95%, Determined by SDS-PAGE
聚合	<5%, Determined by SEC
无菌处理	0.2 µm filtration
纯化方式	Protein G
RRID	AB_10950145
分子量	150 kDa
小鼠病原检测	Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative
保存条件	抗体原液保存在4°C，不能冷冻保存。
推荐同型对照	InVivoPlus rat IgG2b isotype control, anti-keyhole limpet hemocyanin（货号BP0090）
推荐抗体稀释液	InVivoPure pH 7.0 Dilution Buffer（货号IP0070）

为什么选择InVivoPlus抗体？

InVivoPlus级别的产品内毒素含量更低，经过多种实验验证，更适合用于体内实验研究

BioXcell热销产品--InVivoPlus anti-mouse CD8α

该产品自上市已被多篇SCI文献引用，品质有保证，以下是部分已发表的文献引用：

应用

文章

体内CD8+T细胞耗竭

(in vivo CD8+ T cell depletion)

1. Vashist, N., et al. (2018). ‘Influenza-Activated ILC1s Contribute to Antiviral Immunity Partially Influenced by Differential GITR Expression’ Front Immunol 9: 505.

2. Triplett, T. A., et al. (2018). ‘Reversal of indoleamine 2,3-dioxygenase-mediated cancer immune suppression by systemic kynurenine depletion with a therapeutic enzyme’ Nat Biotechnol 36(8): 758-764.

3. Carmi, Y., et al. (2015). ‘Allogeneic IgG combined with dendritic cell stimuli induce antitumour T-cell immunity’ Nature 521(7550): 99-104.

4. Burrack, K. S., et al. (2015). ‘Myeloid Cell Arg1 Inhibits Control of Arthritogenic Alphavirus Infection by Suppressing Antiviral T Cells’ PLoS Pathog 11(10): e1005191.

5. Wensveen, F. M., et al. (2015). ‘NK cells link obesity-induced adipose stress to inflammation and insulin resistance’ Nat Immunol 16(4): 376-385.

6. Li, Z., et al. (2015). ‘Pre-treatment of allogeneic bone marrow recipients with the CXCR4 antagonist AMD3100 transiently enhances hematopoietic chimerism without promoting donor-specific skin allograft tolerance’ Transpl Immunol 33(2): 125-129.

更多产品详情请咨询 BioXcell 中国代理——上海金畔生物

BioXcell热销产品推荐–InVivoPlus anti-mouse Ly6G

发表于2024年6月11日由上海金畔生物科技有限公司

BioXcell热销产品推荐–InVivoPlus anti-mouse Ly6G

产品介绍

BioXcell InVivoPlus anti-mouse Ly6G 1A8单克隆抗体与小鼠Ly6G反应。Ly6G分子量为21-25kDa，是GPI锚定的细胞表面蛋白Ly-6超家族的成员，在细胞信号传导和细胞粘附中发挥作用。Ly6G在发育过程中由骨髓谱系中的细胞（包括单核细胞、巨噬细胞、粒细胞和嗜中性粒细胞）差异表达。单核细胞通常在发育过程中瞬时表达Ly6G，而成熟的粒细胞和外周嗜中性粒细胞保持表达，使Ly6G成为这些群体的良好细胞表面标志物。与BioXcell RB6-8C5抗体不同，1A8抗体与小鼠Ly6G特异性反应，而与Ly6C没有交叉反应性的报道。

BioXcell热销产品推荐--InVivoPlus anti-mouse Ly6G

产品详情

产品名称	InVivoPlus anti-mouse Ly6G
产品货号	BP0075-1
产品规格	5/25/50/100mg
反应种属	Mouse
克隆号	1A8
同种型	Rat IgG2a, κ
免疫原	EL4J cells transfected with Ly6G
实验应用	in vivo neutrophil depletion in vivo MDSC depletion Immunofluorescence Immunohistochemistry (paraffin) Immunohistochemistry (frozen) Flow cytometry
产品形式	PBS, pH 7.0,Contains no stabilizers or preservatives
纯度	>95%, Determined by SDS-PAGE
聚合	<5%, Determined by SEC
无菌处理	0.2 µm filtration
纯化方式	Protein G
RRID	AB_1107721
分子量	150 kDa
小鼠病原检测	Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative
保存条件	抗体原液保存在4°C，不能冷冻保存。
推荐同型对照	InVivoPlus rat IgG2a isotype control, anti-trinitrophenol（货号BP0089）
推荐抗体稀释液	InVivoPure pH 7.0 Dilution Buffer（货号IP0070）

为什么选择InVivoPlus抗体？

InVivoPlus级别的产品内毒素含量更低，经过多种实验验证，更适合用于体内实验研究

BioXcell热销产品推荐--InVivoPlus anti-mouse Ly6G

该产品自上市已被多篇SCI文献引用，品质有保证，以下是部分已发表的文献引用：

应用	文章
体内中性粒细胞耗竭 (in vivo neutrophil depletion)	1. Davis, R. W. t., et al. (2018). ‘Luminol Chemiluminescence Reports Photodynamic Therapy-Generated Neutrophil Activity In Vivo and Serves as a Biomarker of Therapeutic Efficacy’ Photochem Photobiol . 2. Moynihan, K. D., et al. (2016). ‘Eradication of large established tumors in mice by combination immunotherapy that engages innate and adaptive immune responses’ Nat Med. doi : 10.1038/nm.4200. 3. Conde, P., et al. (2015). ‘DC-SIGN(+) Macrophages Control the Induction of Transplantation Tolerance’ Immunity 42(6): 1143-1158. 4. Griseri, T., et al. (2015). ‘Granulocyte Macrophage Colony-Stimulating Factor-Activated Eosinophils Promote Interleukin-23 Driven Chronic Colitis’ Immunity 43(1): 187-199. 5. Yamada, D. H., et al. (2015). ‘Suppression of Fcgamma-receptor-mediated antibody effector function during persistent viral infection’ Immunity 42(2): 379-390. 6. Ellis, G. T., et al. (2015). ‘TRAIL+ monocytes and monocyte-related cells cause lung damage and thereby increase susceptibility to influenza-Streptococcus pneumoniae coinfection’ EMBO Rep 16(9): 1203-1218. 7. Deshmukh, H. S., et al. (2014). ‘The microbiota regulates neutrophil homeostasis and host resistance to Escherichia coli K1 sepsis in neonatal mice’ Nat Med 20(5): 524-530.
体内中性粒细胞耗竭、流式细胞术、免疫组化石蜡切片(in vivo neutrophil depletion, Flow Cytometry, Immunohistochemistry (paraffin))	Coffelt, S. B., et al. (2015). ‘IL-17-producing gammadelta T cells and neutrophils conspire to promote breast cancer metastasis’ Nature 522(7556): 345-348.
体内中性粒细胞耗竭、流式细胞术、免疫组化石蜡切片、免疫组化冰冻切片(in vivo neutrophil depletion, Flow Cytometry, Immunohistochemistry (paraffin), Immunohistochemistry (frozen))	Finisguerra, V., et al. (2015). ‘MET is required for the recruitment of anti-tumoural neutrophils’ Nature 522(7556): 349-353.
体内中性粒细胞耗竭、流式细胞术(in vivo neutrophil depletion, Flow Cytometry)	1.Moser, E. K., et al. (2014). ‘Late engagement of CD86 after influenza virus clearance promotes recovery in a FoxP3+ regulatory T cell dependent manner’ PLoS Pathog 10(8): e1004315. 2. Chen, K. W., et al. (2014). ‘The neutrophil NLRC4 inflammasome selectively promotes IL-1beta maturation without pyroptosis during acute Salmonella challenge’ Cell Rep 8(2): 570-582. 3. Garraud, K., et al. (2012). ‘Differential role of the interleukin-17 axis and neutrophils in resolution of inhalational anthrax’ Infect Immun 80(1): 131-142. 4. Lee, W. B., et al. (2012). ‘Neutrophils Promote Mycobacterial Trehalose Dimycolate-Induced Lung Inflammation via the Mincle Pathway’ PLoS Pathog 8(4): e1002614.
体内骨髓来源抑制细胞耗竭(in vivo MDSC depletion)	Deng, L., et al. (2014). ‘Irradiation and anti-PD-L1 treatment synergistically promote antitumor immunity in mice’ J Clin Invest 124(2): 687-695.
体内中性粒细胞耗竭、免疫荧光(in vivo neutrophil depletion,Immunofluorescence)	Edelson, B. T., et al. (2011). ‘CD8alpha(+) dendritic cells are an obligate cellular entry point for productive infection by Listeria monocytogenes’ Immunity 35(2): 236-248.

更多产品详情请咨询 BioXcell 中国代理——上海金畔生物

InVivoPlus anti-mouse/human/rat/monkey/hamster/canine/bovine TGF-β

发表于2024年6月8日由上海金畔生物科技有限公司

InVivoPlus anti-mouse/human/rat/monkey/hamster/canine/bovine TGF-β

克隆号1D11.16.8单克隆抗体能与小鼠、人、大鼠、猴、仓鼠、犬和牛TGF-β(转化生长因子β)亚型1、2和3反应。TGF-β是一种多功能细胞因子，调节上皮细胞、内皮细胞、成纤维细胞、神经元、包括T淋巴细胞和NK细胞在内的淋巴细胞以及其他造血细胞类型的增殖。TGF-β还调节活化巨噬细胞的活性和调节性T细胞的发育。此外，TGF-β在免疫功能、组织重塑和伤口修复中发挥作用。TGF-β以五种高度相似的亚型(TGF-β 1-5)存在，同源性为70-80%。TGF-β1是通过酶切由TGFB1基因编码的长前体TGF-β1多肽合成的，该多肽产生成熟蛋白和潜伏期相关肽(LAP)。LAP和成熟的TGF-β1在分泌过程中非共价结合。TGF-β在许多细胞类型中普遍表达，包括巨噬细胞和血小板，它们表达高水平的TGF-β。TGF-β信号已被证明在癌症、自身免疫性疾病、哮喘、心脏病和糖尿病中发挥作用。TGF-β基因敲除小鼠表现出造血和内皮分化缺陷，并死于严重炎症，这说明了它的重要性。克隆号1D11.16.8单克隆抗体是一种中和抗体。

产品详情

产品货号	BP0057
产品规格	1/5/25/50/100mg
抗体亚型	Mouse IgG1, κ
推荐同型对照	InVivoPlus™ mouse IgG1 isotype control, unknown specificity（货号：BP0083）
推荐抗体稀释液	InVivoPure™ pH 7.0 Dilution Buffer（货号：IP0070）
免疫原	Bovine TGFβ isoform 2
应用	in vivo TGFβ neutralization in vitro TGFβ neutralization Western blot
产品形式	PBS , pH 7.0 Contains no stabilizers or preservatives
内毒素水平	<1eu> 使用 LAL gel clotting 测定
聚集体	<5% Determined by DLS
纯度	>95% Determined by SDS-PAGE
无菌处理	0.2 μM filtered
生产形式	从组织培养上清液中纯化得到。
纯化形式	Protein G
RRID	AB_1107757
分子量大小	150 kDa
鼠病原体测试结果	Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Minute Virus: Negative Mouse Hepatitis Virus: Negative Reovirus Screen: Negative Lymphocytic Choriomeningitis virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Mouse Rotavirus: Negative Theiler’s Murine Encephalomyelitis: Negative Ectromelia/Mousepox Virus: Negative Hantavirus: Negative Polyoma Virus: Negative Mouse Adenovirus: Negative Sendai Virus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Mouse Cytomegalovirus: Negative K Virus: Negative
保存条件	抗体原溶液应保存在4°C条件下，不要冷冻保存。

已发表参考文献：

in vivo TGFβ neutralization	Komai, T., et al. (2018). “Transforming Growth Factor-beta and Interleukin-10 Synergistically Regulate Humoral Immunity via Modulating Metabolic Signals.” Front Immunol 9: 1364.
in vitro TGFβ neutralization	Bodogai, M., et al. (2015). “Immunosuppressive and Prometastatic Functions of Myeloid-Derived Suppressive Cells Rely upon Education from Tumor-Associated B Cells.” Cancer Res 75(17): 3456-3465.
in vitro TGFβ neutralization	Choi, Y. S., et al. (2015). “LEF-1 and TCF-1 orchestrate TFH differentiation by regulating differentiation circuits upstream of the transcriptional repressor Bcl6.” Nat Immunol 16(9): 980-990.

详情请咨询 BioXcell 中国代理-上海金畔生物科技

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标签归档：invivoplus

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BioXcell热销产品–InVivoPlus rat IgG2a isotype control

BioXcell热销产品推荐–InVivoPlus anti-mouse PD-L1 (B7-H1)

BioXcell热销产品–InVivoPlus anti-mouse CD8α

BioXcell热销产品推荐–InVivoPlus anti-mouse Ly6G

InVivoPlus anti-mouse/human/rat/monkey/hamster/canine/bovine TGF-β

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