Epicypher热销产品——Histone H3K4me3 Antibody, SNAP-Certified™ for CUT&RUN and ChIP

发表于2024年12月15日由上海金畔生物科技有限公司

EpiCypher是一家为表观遗传学和染色质生物学研究提供高质量试剂和工具的专业制造商。EpiCypher推出的CUT&RUN和ChIP级别的Histone H3K4me3 Antibody符合EpiCypher的批次特异性SNAP-CertifiedTM标准，在CUT&RUN和ChIP的应用中具有高特异性和高效的靶标富集能力。在CUT&RUN中，使用SNAP-CUTANATM K-MetStat Panel测定对照组的峰值(EpiCypher 19-1002，Fig.1)，相关组蛋白PTMs的交叉反应性<20%。在500k和50k起始细胞中一致的基因组富集证实了高靶标效率(Fig. 2-4)。在ChIP中，使用SNAP-ChIP®K-MetStat Panel测定对照组的峰值 (EpiCypher 19-1001，Fig. 7)，相关组蛋白PTMs的交叉反应性<20%，并且确定目标输入回收率>5%。

产品详情

反应种属：Human, Mouse, Drosophila, Yeast, Wide Range (Predicted)

宿主来源：Rabbit

实验应用：CUT&RUN, ChIP, ICC/IF, WB

免疫原：A synthetic peptide corresponding to histone H3 trimethylated at lysine 4

克隆性：Mixed Monoclonal*

*Mixed Monoclonal: a pool of multiple monoclonal antibodies.

保存温度：Stable for 1 year at -20°C from date of receipt

运输温度：Frozen cold packs.

产品形式：Protein A affinity-purified antibody in PBS pH 7.4, 0.09% sodium azide

数据示例

Epicypher热销产品——Histone H3K4me3 Antibody, SNAP-Certified™ for CUT&RUN and ChIP

FIGURE 1 SNAP specificity analysis in CUT&RUN. CUT&RUN was performed as described above. CUT&RUN sequencing reads were aligned to the unique DNA barcodes corresponding to each nucleosome in the K-MetStat panel (x-axis). Data are expressed as a percent relative to on-target recovery (H3K4me3 set to 100%).

Epicypher热销产品——Histone H3K4me3 Antibody, SNAP-Certified™ for CUT&RUN and ChIP

FIGURE 2 CUT&RUN genome wide enrichment. CUT&RUN was performed as described above. Sequence reads were aligned to 18,793 annotated transcription start sites (TSSs ± 2 kbp). Signal enrichment was sorted from highest to lowest (top to bottom) relative to the H3K4me3 – 50k cells sample (all gene rows aligned). High, medium, and low intensity are shown in red, yellow, and blue, respectively. H3K4me3 antibody produced the expected TSS enrichment pattern, which was consistent between 500k and 50k cells and greater than the IgG negative control.

Epicypher热销产品——Histone H3K4me3 Antibody, SNAP-Certified™ for CUT&RUN and ChIP

FIGURE 3 H3K4me3 CUT&RUN representative browser tracks. CUT&RUN was performed as described above. Gene browser shots were generated using the Integrative Genomics Viewer (IGV, Broad Institute). Similar results in peak structure and location were observed for both cell inputs.

Epicypher热销产品——Histone H3K4me3 Antibody, SNAP-Certified™ for CUT&RUN and ChIP

FIGURE 4 Antibody efficiency analysis in CUT&RUN using cell input correlation. CUT&RUN was performed as described above. Genome-wide correlation analysis was performed to compare H3K4me3 antibody enrichment using 500k and 50k cell inputs. The log of the number of reads per 75 bp binned region across the genome is plotted for both samples. CUT&RUN data generated using this H3K4me3 antibody are highly correlated between the two cell inputs (Pearson correlation r = 0.970), indicating high efficiency of H3K4me3 antibody target recovery.

Epicypher热销产品——Histone H3K4me3 Antibody, SNAP-Certified™ for CUT&RUN and ChIP

FIGURE 5 Immunofluorescence. Representative images (60X magnification) of HeLa cells fixed, permeabilized, and immunostained to show endogenous localization of H3K4me3. (A) H3K4me3 antibody (green, 1:100 dilution) detected with an Alexa Fluor® 488 conjugated anti-rabbit secondary. (B) DAPI stained nuclei (blue). (C) Rhodamine stained cytoskeletal F-actin (red). (D) A composite of panels a, b, & c demonstrating nuclear localization of H3K4me3. (E) Negative control lacking H3K4me3 primary antibody to assess background.

Epicypher热销产品——Histone H3K4me3 Antibody, SNAP-Certified™ for CUT&RUN and ChIP

FIGURE 6 Western blot data. Western analysis of H3K4me3 in whole cell extracts from HeLa, Hep G2, HCT 116, MCF7, U-2 OS, A549, HEK-293, NIH/3T3, and PC-12 cells. 30 µg of lysate was resolved via SDS-PAGE and detected with H3K4me3 antibody at a 1:250 dilution.

Epicypher热销产品——Histone H3K4me3 Antibody, SNAP-Certified™ for CUT&RUN and ChIP

FIGURE 7 SNAP-ChIP-qPCR specificity and enrichment analysis. Histone H3K4me3 antibody (5 µg) was tested in a native ChIP experiment using chromatin from K562 cells (5 µg) with the SNAP-ChIP K-MetStat Panel (EpiCypher 19-1001) spiked-in prior to micrococcal nuclease digestion. Specificity (left y-axis) was determined by qPCR for the DNA barcodes corresponding to modified nucleosomes in the SNAP-ChIP panel (x-axis). Black bar represents antibody efficiency (right yaxis; log scale) and indicates percentage of the target immunoprecipitated relative to input. Error bars represent mean ± SEM in replicate ChIP experiments.

抗体使用文献引用

1. Shah et al.Examining the Roles of H3K4 Methylation States with Systematically Characterized Antibodies. Mol. Cell (2018). PMID: 30244833.

订购详情

货号	产品名称	规格
13-0041	Histone H3K4me3 Antibody, SNAP-Certified™ for CUT&RUN and ChIP	100 µg

如需了解更多详细信息或相关产品，请联系EpiCypher中国代理商-上海金畔生物

H3K36me3 Antibody——EpiCypher新品推荐

发表于2024年12月6日由上海金畔生物科技有限公司

H3K36me3 Antibody——EpiCypher新品推荐

组蛋白修饰是表观遗传调控的一种重要形式。其中，组蛋白甲基化是染色质形态的关键决定因素，参与多个细胞过程。作为保守的组蛋白甲基化标记，组蛋白3赖氨酸36三甲基化(H3K36me3)可以介导多种转录相关事件，如转录活性调节、转录延伸、pre-mRNA选择性剪接和RNA m6甲基化。此外，H3K36me3还有助于DNA损伤修复。鉴于H3K36me3在基因组调控中的关键功能，许多研究都强调了H3K36me3及其唯一的甲基转移酶SETD2在发病机制的作用，尤其是恶性肿瘤。

EpiCypher新推出H3K36me3抗体，使用其独有的SNAP-Certification技术进行验证，符合CUT&RUN和CUT&Tag应用中特异性和有效靶标富集的标准。该抗体靶向在赖氨酸36处三甲基化的组蛋白H3，赖氨酸在活性基因的启动子和基因体中富集。

产品详情

产品名称	H3K36me3 Antibody, SNAP-Certified™ for CUT&RUN
产品货号	13-0058
产品规格	100 µg
类型	Monoclonal [2091-1E2]
反应性	Human, Wide Range (Predicted)
宿主来源	Rabbit
免疫原	A synthetic peptide corresponding to histone H3 trimethylated at lysine 36
实验应用	CUT&RUN
产品形式	Antigen affinity-purified antibody in Borate buffered saline pH 8.0, 0.09% sodium azide
产品浓度	1 mg/mL
建议稀释度	0.5 µg per reaction for CUT&RUN

实例分析

使用SNAP-CUTANA™ K-MetStat Panel对H3K36me3抗体进行分析，抗体的交叉反应性<20%（图1），确保了精确可靠的数据；不同细胞数量下，一致的基因组富集结果证实了其高靶向效率（图2-3）。

H3K36me3 Antibody——EpiCypher新品推荐

图1. CUT&RUN中SNAP特异性分析

H3K36me3 Antibody——EpiCypher新品推荐

图2. CUT&RUN全基因组富集

H3K36me3 Antibody——EpiCypher新品推荐

图3. H3K36me3 CUT&RUN representative browser tracks

关于EpiCypher

EpiCypher是一家成立于2012年的表观遗传学公司。从专有组蛋白肽阵列平台EpiGold™开始，EpiCypher开发了一系列同类产品。同时，EpiCypher是重组核小体制造和开发的全球领导者。利用其独有技术，不断添加高纯度修饰重组核小体（dNucs™）产品。dNuc™多样性的产品为破译组蛋白编码和加速药物开发提供了强大的工具。

EpiCypher还将dNuc™技术广泛的应用于多种分析测定产品中，包括：SNAP-ChIP®Spike-in Controls（用于抗体分析和ChIP定量）， EpiDyne®底物（用于染色质重塑和抑制剂筛选及开发），dCyher™测定（用于探究表观遗传蛋白质-组蛋白PTM结合相互作用）。最近，EpiCypher还推出了针对ChIC、CUT&RUN和CUT&Tag的高灵敏度表观基因组图谱CUTANA™分析。

如需了解更多详细信息或相关产品，请联系EpiCypher中国代理商-上海金畔生物

H3K27me3 Antibody——EpiCypher新品推荐

发表于2024年11月11日由上海金畔生物科技有限公司

H3K27me3 Antibody——EpiCypher新品推荐

H3K27me3是核心组蛋白H3的第27个氨基酸赖氨酸的氨基(N)末端尾部发生的组蛋白甲基化，是最为常见的组蛋白甲基化修饰之一。H3K27me3是具有组蛋白甲酰基转移酶活性的多梳抑制复合体2 (Polycomb Repressive Complex 2,PRC2)的下游靶标，PRC2在干细胞多能性、体细胞分化和增殖中具有不同的作用，PRC2的酶亚基EZH1和EZH2催化H3K27的甲基化，H3K27的甲基化是抑制基因转录的关键介质，参与多个重要的生物学过程。H3K27me3作为一种基因转录抑制因子，在胚胎发生和肿瘤形成中具有多种作用。H3K27me3的缺失发生在恶性周围神经鞘膜瘤(MPNST)的一个重要亚群中，其他肿瘤也可能表现为H3K27me3表达缺失，如胚胎性横纹肌肉瘤、脑膜瘤、放射相关性未分类肉瘤、放射相关性血管肉瘤、去分化软骨肉瘤、黑色素瘤和默克尔细胞癌等。因此H3K27me3可以作为恶性周围神经鞘膜瘤的鉴别指标。

H3K27me3抗体是EpiCypher最新研究的SNAP-Certified™抗体之一，并通过了独有的SNAP-Certification技术进行验证，符合CUT&RUN和CUT&Tag应用中特异性和有效靶标富集的标准。

产品详情

产品货号	13-0055
产品名称	H3K27me3 Antibody, SNAP-Certified™ for CUT&RUN and CUT&Tag
规格	100 µg
产品类型	Monoclonal [2084-1G5]
宿主	Rabbit
浓度	0.5 mg/mL
反应种属	Human, Wide Range(Predicted)
实验应用	CUT&RUN, CUT&Tag
免疫原	A synthetic peptide corresponding to histone H3 trimethylated at lysine 27
保存温度	Stable for 1 year at 4°C from date of receipt
配方	Antigen affinity-purified antibody in Borate buffered saline pH 8.0, 0.09% sodium azide
建议稀释度	0.5 µg per reaction for CUT&RUN and CUT&Tag

实例分析

H3K27me3 Antibody——EpiCypher新品推荐

图1. CUT&RUN中SNAP特异性分析

H3K27me3 Antibody——EpiCypher新品推荐

图2. CUT&Tag中SNAP特异性分析

使用SNAP-CUTANA™ K-MetStat Panel对H3K27me3抗体进行分析，其与相关组蛋白PTMs的交叉反应性<20%。

H3K27me3 Antibody——EpiCypher新品推荐

图3. CUT&RUN和CUT&Tag全基因组富集

H3K27me3与基因的抑制有关，而H3K4me3一般在转录起始位点附近富集，来激活基因转录。

H3K27me3 Antibody——EpiCypher新品推荐

图4. H3K27me3抗体CUT&RUN和CUT&Tag峰图

高效抗体在代表性基因上表现出相似的峰值结构，即使在细胞数量减少的情况下，全基因组信号也高度稳定。

H3K27me3抗体在CUT&RUN和CUT&Tag应用中符合EpiCypher特定批次SNAP-Certified™标准，不同的细胞数量下，一致的基因组富集证实了其高靶向效率。SNAP-Certified™抗体具有交叉反应性低、CUT&RUN和CUT&Tag性能稳定、改善低细胞数的PTM分析等特点。

关于EpiCypher

如需了解更多详细信息或相关产品，请联系EpiCypher中国代理商-上海金畔生物

EpiCypher新品推荐——H3K4me3 Antibody, SNAP-Certified™ for CUT&RUN and CUT&Tag

发表于2024年11月9日由上海金畔生物科技有限公司

EpiCypher新品推荐——H3K4me3 Antibody, SNAP-Certified™ for CUT&RUN and CUT&Tag

H3K4me3(组蛋白H3赖氨酸4三甲基化)抗体符合EpiCypher的批次特异性SNAP-Certified™标准，在CUT&RUN和CUT&Tag应用中具有特异性和高效的靶标富集。这需要与相关组蛋白 PTMs （使用加标对照的 SNAP-CUTANA™ K-AcylStat Panel 测定，EpiCypher 19-1002）的交叉反应性 <20%（图1和图5）。在不同的细胞起始条件下，一致的基因组富集结果证实了高靶标效率：CUT&RUN中500k和50k的细胞量(图2-3)，CUT&Tag中100k和10k的细胞量(图6-7)。即使在细胞数量减少的情况下，高效抗体也显示出相似的峰结构(图3和7)和高度保守的全基因组信号(图2和6)。该抗体靶向组蛋白H3K4me3，其在转录起始位点（TSS）附近的活性启动子处富集并促进基因激活。

产品详情

产品名称：H3K4me3 Antibody, SNAP-Certified™ for CUT&RUN and CUT&Tag

宿主来源：Rabbit

实验应用：CUT&RUN, CUT&Tag

免疫原：A synthetic peptide corresponding to histone H3 trimethylated at lysine 4

克隆性：Monoclonal[2909-3D7]

保存温度：自收到之日起，4℃下可稳定储存1年。

验证数据

—— CUT&RUN

EpiCypher新品推荐——H3K4me3 Antibody, SNAP-Certified™ for CUT&RUN and CUT&Tag

Figure 1: SNAP specificity analysis in CUT&RUN

CUT&RUN was performed as described in Figure 4. CUT&RUN sequencing reads were aligned to the unique DNA barcodes corresponding to each nucleosome in the K-MetStat panel (x-axis). Data are expressed as a percent relative to on-target recovery (H3K4me3 set to 100%). The antibody showed highly specific recovery of H3K4me3 spike-in nucleosomes at both 500k and 50k cells.

EpiCypher新品推荐——H3K4me3 Antibody, SNAP-Certified™ for CUT&RUN and CUT&Tag

Figure 2: CUT&RUN genome-wide enrichment

CUT&RUN was performed as described in Figure 4. Sequence reads were aligned to 18,793 annotated transcription start sites (TSSs ± 2 kbp). Signal enrichment was sorted from highest to lowest (top to bottom) relative to the H3K4me3 – 500k cells reaction (all gene rows aligned). High, medium, and low intensity are shown in red, yellow, and blue, respectively. H3K4me3 antibodies produced the expected enrichment pattern, which was consistent between 500k and 50k cells and greater than the IgG negative control.

EpiCypher新品推荐——H3K4me3 Antibody, SNAP-Certified™ for CUT&RUN and CUT&Tag

Figure 3: H3K4me3 CUT&RUN representative browser tracks

CUT&RUN was performed as described in Figure 4. Gene browser shots were generated using the Integrative Genomics Viewer (IGV, Broad Institute). H3K4me3 antibody tracks display sharp peaks at gene promoters, consistent with the biological function of this PTM. Similar results in peak structure and location were observed for both 500k and 50k cell inputs.

EpiCypher新品推荐——H3K4me3 Antibody, SNAP-Certified™ for CUT&RUN and CUT&Tag

Figure 4: CUT&RUN methods

CUT&RUN was performed on 500k and 50k K562 cells with the SNAP-CUTANA™ K-MetStat Panel (EpiCypher 19-1002) spiked-in prior to the addition of 0.5 µg of either IgG negative control (EpiCypher 13-0042) or H3K4me3 antibodies. The experiment was performed using the CUTANA™ ChIC/CUT&RUN Kit v3 (EpiCypher 14-1048). Library preparation was performed with 5 ng of CUT&RUN enriched DNA (or the total amount recovered if less than 5 ng) using the CUTANA™ CUT&RUN Library Prep Kit (EpiCypher 14-1001/14-1002). Both kit protocols were adapted for high throughput Tecan liquid handling. Libraries were run on an Illumina NextSeq2000 with paired-end sequencing (2×50 bp). Sample sequencing depth was 3.5 million reads (IgG 500k cell input), 4.0 million reads (IgG 50k cell input), 5.6 million reads (H3K4me3 500k cell input), and 2.8 million reads (H3K4me3 50k cell input). Data were aligned to the hg19 genome using Bowtie2. Data were filtered to remove duplicates, multi-aligned reads, and ENCODE DAC Exclusion List regions.

—— CUT&Tag

EpiCypher新品推荐——H3K4me3 Antibody, SNAP-Certified™ for CUT&RUN and CUT&Tag

Figure 5: SNAP specificity analysis in CUT&Tag

CUT&Tag was performed as described in Figure 8. CUT&Tag sequencing reads were aligned to the unique DNA barcodes corresponding to each nucleosome in the K-MetStat panel (x-axis). Data are expressed as a percent relative to on-target recovery (H3K4me3 set to 100%). The antibody showed highly specific recovery of H3K4me3 spike-in nucleosomes at both 100k and 10k nuclei.

EpiCypher新品推荐——H3K4me3 Antibody, SNAP-Certified™ for CUT&RUN and CUT&Tag

Figure 6: CUT&Tag genome-wide enrichment

CUT&Tag was performed as described in Figure 8. Sequence reads were aligned to 18,793 annotated transcription start sites (TSSs ± 2 kbp). Signal enrichment was sorted from highest to lowest (top to bottom) relative to the H3K4me3 – 100k nuclei reaction (all gene rows aligned). High, medium, and low intensity are shown in red, yellow, and blue, respectively. H3K4me3 antibodies produced the expected enrichment pattern, which was consistent between 100k and 10k nuclei and greater than the IgG negative control.

EpiCypher新品推荐——H3K4me3 Antibody, SNAP-Certified™ for CUT&RUN and CUT&Tag

Figure 7: H3K4me3 CUT&Tag representative browser tracks

CUT&Tag was performed as described in Figure 8. Gene browser shots were generated using the Integrative Genomics Viewer (IGV, Broad Institute). H3K4me3 antibody tracks display sharp peaks at gene promoters, consistent with the biological function of this PTM. Similar results in peak structure and location were observed for both 100k and 10k nuclei inputs.

EpiCypher新品推荐——H3K4me3 Antibody, SNAP-Certified™ for CUT&RUN and CUT&Tag

Figure 8: CUT&Tag methods

CUT&Tag was performed on 100k and 10k K562 nuclei with the SNAP-CUTANA™ K-MetStat Panel (EpiCypher 19-1002) spiked-in prior to the addition of 0.5 µg of either IgG negative control (EpiCypher 13-0042) or H3K4me3 antibodies. The experiment was performed using the CUTANA™ CUT&Tag Kit v1 (EpiCypher 14-1102/14-1103). Libraries were run on an Illumina NextSeq2000 with paired-end sequencing (2×50 bp). Sample sequencing depth was 1.3 million reads (IgG 100k nuclei input), 2.0 million reads (IgG 10k nuclei input), 6.9 million reads (H3K4me3 100k nuclei input), and 10.6 million reads (H3K4me3 10k nuclei input). Data were aligned to the hg19 genome using Bowtie2. Data were filtered to remove duplicates, multi-aligned reads, and ENCODE DAC Exclusion List regions.

订购详情

货号	产品名称	规格
13-0060	H3K4me3 Antibody, SNAP-Certified™ for CUT&RUN and CUT&Tag	100 µg

如需了解更多详细信息或相关产品，

请联系EpiCypher中国代理商-上海金畔生物

Epicypher热销产品——Nucleosome, Recombinant Human, H3K4me3 dNuc, Biotinylated

发表于2024年6月21日由上海金畔生物科技有限公司

Epicypher热销产品——Nucleosome, Recombinant Human, H3K4me3 dNuc, Biotinylated

EpiCypher是一家为表观遗传学和染色质生物学研究提供高质量试剂和工具的专业制造商。EpiCypher生产的重组单核小体（H3K4me3）由 147 个DNA碱基对缠绕在组蛋白的八聚体核心（H2A、H2B、H3.2 和 H4 各两个）上构成，是染色质的基本单位。Lowary和Widom[1]鉴定的147bp 601序列对组蛋白八聚体具有高亲和力，可用于核小体组装。H3K4me3 dNuc 在组蛋白H3.2的第4位含有三甲基赖氨酸。H3K4me3在110位处有一个Cys到Ala的取代。该DNA含有一个5’biotin-TEG基团。EpiCypher生产的重组单核小体适用于多种应用，包括用作酶分析、高通量筛选和抑制剂测试、染色质结合研究、蛋白质-蛋白质相互作用分析、结构研究和效应蛋白结合实验中的底物。

[1] Lowary and Widom J. Mol. Biol. (1998). PMID: 9514715

产品详情

宿主来源: Human

表达系统: E. coli & synthetic DNA

标签: Biotinylated

保存温度: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws

产品形式: 0.977 mg/mL mononucleosome in 51.2 µL 10 mM Tris HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol (27.3 µg protein, 50 µg DNA + protein)

验证数据

	Figure 1: Western blot data Western Analysis of H3K4me3 dNuc. Top Panel: Unmodified (EpiCypher 16-0006; Lane 1) and H3K4me3 (Lane 2) nucleosomes were probed with an anti-H3K4me3 antibody and analyzed via ECL readout. Only the H3K4me3 sample produced a detectable signal. Bottom Panel: Detail from Coomassie stained gel showing unmodified (Lane 1) and H3K4me3 (Lane 2) nucleosomes.

Figure 2: Mass spec data Synthetic H3K4me3 histone analyzed by high resolution mass spectrometry. Expected mass = 15,267.77 Da. Determined mass = 15,266.15 Da.
	Figure 3: Protein gel data Coomassie stained SDS-PAGE gel of proteins in H3K4me3 dNuc (1 µg) demonstrates the purity of histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H3K4me3 and H4) are indicated.
	Figure 4: DNA gel data H3K4me3 dNuc resolved via native PAGE gel and stained with ethidium bromide to visualize DNA. Lane 1: Free DNA (EpiCypher 18-0005; 100 ng). Lane 2: Intact H3K4me3 nucleosomes (400 ng).

订购详情

货号	产品名称	规格
16-0316	Nucleosome, Recombinant Human, H3K4me3 dNuc, Biotinylated	50 µg
16-0316-20	Nucleosome, Recombinant Human, H3K4me3 dNuc, Biotinylated	20 µg

如需了解更多详细信息或相关产品，请联系EpiCypher中国代理商-上海金畔生物

H3K4me1 Antibody——EpiCypher新品推荐

发表于2024年6月12日由上海金畔生物科技有限公司

H3K4me1 Antibody——EpiCypher新品推荐

组蛋白八聚体由组蛋白H2A、H2B、H3和H4的各两个亚基组成，由DNA包裹形成核小体。组蛋白亚基的N末端含有可进行翻译后修饰的残基。H3上的赖氨酸残基K4可以单、二或三甲基化，分别形成H3K4me1、H3K4me2或H3K4me3。H3K4me1、H3K4me2和H3K4me3位于整个基因组的不同区域。H3K4me1修饰主要分布在活性增强子元件中；H3K4me2修饰主要分布在转录活跃基因的gene body区；而H3K4me3修饰主要分布在TSS附近的启动子区并激活转录。

H3K4me1 Antibody——EpiCypher新品推荐

图片来源: PMID: 30616667

关于H3K4me1

H3K4me1是组蛋白H3蛋白的第4个赖氨酸残基处的单甲基化，通常与基因增强子相关。H3K4me1富集在活性和primed增强子区域内。增强子由组蛋白H3K4单/二甲基转移酶MLL4引发，然后由组蛋白H3K27乙酰转移酶p300激活。H3K4me1会微调增强子的活性和功能，而不是控制。具有MLL3/4的H3K4me1也可以作用于启动子并抑制基因。H3K4me1修饰是真核细胞增强子的表观遗传学标记，可用于增强子在基因组上定位。

EpiCypher H3K4me1抗体

EpiCypher在CUT&RUN和CUT&Tag实验中筛选了数百种抗体，并通过独有的SNAP-Certification技术进行验证，为研究者提供了可识别经过修饰的组蛋白和相关细胞靶点的组蛋白PTM抗体。H3K4me1抗体是EpiCypher最新推出的SNAP-Certified™抗体之一，符合CUT&RUN和CUT&Tag应用中特异性和有效靶标富集的标准。

产品详情

产品货号	13-0057
产品名称	H3K4me1 Antibody, SNAP-Certified™ for CUT&RUN and CUT&Tag
规格	100 µg
产品类型	Monoclonal [2088-1F4]
宿主	Rabbit
浓度	0.5 mg/mL
反应种属	Human, Wide Range(Predicted)
实验应用	CUT&RUN, CUT&Tag
免疫原	A synthetic peptide corresponding to histone H3 monomethylated at lysine 4
保存温度	Stable for 1 year at 4°C from date of receipt
配方	Antigen affinity-purified antibody in Borate buffered saline pH 8.0, 0.09% sodium azide
建议稀释度	0.5 µg per reaction for CUT&RUN and CUT&Tag