Figure 1: DNA Gel Data

EpiDyne-FRET substrate resolved via native PAGE gel and 

stained with ethidium bromide to visualize DNA. Lane 1: Free 

DNA (100 ng). Lane 2: Intact EpiDyne-FRET nucleosomes 

(400 ng).

Figure 2: Protein Gel Data

Coomassie stained PAGE gel of proteins in EpiDyne-FRET 

substrate (1 µg) demonstrates the purity of histones in the 

preparation. Sizes of molecular weight markers and positions

of the core histones (H2A-Cy5, H2B, H3.2, and H4) are 

indicated.

Figure 3: Nucleosome Remodeling Data

ACF1/ATP-dependent nucleosome remodeling reaction. EpiDyne-FRET 

nucleosomes (15 nM) were incubated with ACF1 chromatin remodeler

 (EpiCypher 15-1013; indicated concentrations) in the presence or absence 

of 1 mM ATP. Upon the addition of ATP, reactions were immediately read 

in an Envision Multilabelplate reader (PerkinElmer). Data are presented 

as the mean of the Cy3/Cy5 ratio (N=2).

货号

产品名称

规格

16-4201   

EpiDyne-FRET Nucleosome Remodeling Assay Substrate   

50 µg