CleanCap® EGFP mRNA – (L-7601)产品信息

CleanCap® EGFP mRNA – (L-7601)产品信息

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EGFP mRNA表达了一个增强型绿色荧光蛋白,该蛋白最初从维多利亚水母中获得。EGFP是一种哺乳动物细胞中常用的可直接检测的报告基因,可产生明亮的绿色荧光,发射峰为509nm。

EGFP mRNA使用了TriLink特有的共转录加帽试剂CleanCap进行加帽,该加帽试剂可产生Cap 1结构,并具有高效的加帽效率。EGFP mRNA是多聚腺苷酸化的,并针对哺乳动物系统进行了优化,模仿完全加工的成熟mRNA。

产品信息:

货号:L-7601

规格:100 µg,1 mg,5 x 1 mg

纯度:通过琼脂糖凝胶迁移实验

保存温度:-40℃或以下

长度:996个核苷酸

溶液:1 mM 柠檬酸钠溶液,pH 6.4

浓度:1.0 mg/ml

碱基组成:未修饰的碱基

换算系数:40 µg/OD260

应用:报告基因

QC检测:特性与纯度

              琼脂糖凝胶迁移率:通过 

              浓度: ± 6% 通过

CleanCap® EGFP mRNA – (L-7601)产品信息图文对照:

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Enhanced Green Fluorescent Protein, EGFP (200-208)

Enhanced Green Fluorescent Protein, EGFP (200-208)分子量: 1019.1描述序列纯度储存/保存方法别名参考文献

产品描述
描述

This peptide is H2-Kd-restricted enhanced green fluorescent protein (EGFP)-derived peptide (200-208) and represents a CD8 T cell epitope. Immunization with this peptide stimulates IFNg production, thus making this peptide a model tumor antigen for the experimental development of antigen-specific vaccines against cancer.

序列
H – His – Tyr – Leu – Ser – Thr – Gln – Ser – Ala – Leu – OH
纯度
≥ 95%
储存/保存方法
-20°C
基本信息
别名
HYLSTQSAL
参考文献
参考文献
Wysocki, P. et al. Clin Cancer Res 12, 4095 (2006); Sharma, S. et al. J Immunol 177, 8348 (2006); Wingender, G. et al. Eur J Immunol 36, 12 (2006); Gambotto, A. et al. Gene Ther 7, 2036 (2000).

Recombinant EGFP-Tag protein

Recombinant EGFP-Tag protein

货号:
P06417

品牌:
Jinpan

产品简介
基因名 EGFP-Tag
英文全称 Enhanced green fluorescent protein
别称 /
中文名称 增强型绿色荧光蛋白(His标签)
Uniprot /
GeneID /
交叉反应(推测) /
氨基酸大小 27kDa
表达氨基酸 MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK
Background /
Application Immunology research
Derived from E.coli
Purification NI-NTA affinity purification
Formulation Lyophilized from a 0.2 μm filtered solution of Tris-HCl, PH8.0.
Shipping The product is shipped at -20°C temperature. Upon receipt, store it immediately at the temperature listed below.
Storage Reconstituted protein solution can be stored at 4-7°C for 2-3 months, stable at < -20°C for 1-2 years.
Reconstitution Reconstituted protein solution can be diluted with distilled PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Purity Purity≥85% as determined by reducing SDS-PAGE.
Endotoxin /
Bioactive Unknown. (Ban’t be used in biological activity experiments such as cell culture, treatment and diagnosis.)
规格 10ug 50ug 100ug 1mg

Recombinant EGFP protein

Recombinant EGFP protein

货号:
P02339

品牌:
Jinpan

产品简介
基因名 EGFP
英文全称 Enhanced Green Fluorescent Protein
别称 /
中文名称 增强型绿色荧光蛋白
Uniprot P42212
GeneID 107331116
交叉反应(推测) Jellyfish
氨基酸大小 27kDa
表达氨基酸 1-239aa
Background This gene encodes a GTP binding protein and is located in the pseudoautosomal region (PAR) at the end of the short arms of the X and Y chromosomes.
Application Immunology research
Derived from E.coli
Purification NI-NTA affinity purification
Formulation Lyophilized from a 0.2 μm filtered solution of Tris-HCl, PH8.0.
Shipping The product is shipped at -20°C temperature. Upon receipt, store it immediately at the temperature listed below.
Storage Reconstituted protein solution can be stored at 4-7°C for 1-2 months, stable at < -20°C for 2-3 years.
Reconstitution Reconstituted protein solution can be diluted with distilled PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Purity Purity≥85% as determined by reducing SDS-PAGE.
Bioactive Unknown. (Ban’t be used in biological activity experiments such as cell culture, treatment and diagnosis.)
规格 10ug 50ug 100ug 1mg