Detroit R&D——14,15-DHET sEH activity ELISA Kit

Detroit R&D——14,15-DHET sEH activity ELISA Kit

货号DT-SH1

名称:14,15-DHET sEH activity ELISA Kit

规格:1 kit


产品描述:

14,15-dhet是一种由可溶性环氧化物水解酶(seh)介导的14,15-eet代谢形成的代谢产物,由细胞色素p450cyps2c2j的花生四烯酸环氧合酶活性产生,该竞争性酶联免疫吸附测定试剂盒可用于测定14,15-生物样品和细胞培养基中的DHET水平作为SEH活性的测量。此外,该方法也可用于筛选可溶性环氧化物水解酶活性抑制剂。该试剂盒与我们的14,15-dhet/eet-elisa试剂盒(dh 2)非常相似,但同时也提供了14,15-eet底物,因此我们可以通过可溶性环氧化物水解酶来测量eetdhet的代谢活性或抑制作用。该试剂盒采用96孔板格式,含有足够的14,15-EET,可用于12种单独的反应混合物。

检测样本:cellstissues、

保存温度All components are stored below -20°C and should not be re-frozen and thawed more than necessary.


试剂盒组成:

14,15-DHET ELISA Plate          Solid 96-well plate coated with anti-14,15-DHET antibody in each well

14,15-DHET Standard (2 μL)           Stock standard at a concentration of 1 mg/mL

14,15-DHET-HRP Conjugates (12 μL)          1000 X concentrated solution

Sample Dilution Buffer (25 mL)      10 X solution of Tris-buffered saline with preservatives

HRP Buffer (15 mL)                 1 X solution of Tris-buffered saline with preservatives

Wash Buffer Solution(25mL)        10 X solution of Tris-buffered saline with detergents and preservatives

TMB Substrate (24 mL)                A solution of TMB (tetra methyl benzadine)

14,15-EET (90 uL)                                100um stock solution

 

关于Detroit R&D

Detroit R&D公司由Dr. Hyesook Kim创立于1997年,Detroit R&D通过领先的技术提供优质产品,致力于赢得科研工作者的绝对信任。Detroit R&D的特色产品涉及环境健康科学、高血压、乳腺癌和前列腺癌相关研究领域,包括类花生酸生物活性研究相关产品,如EET,DHET和20-HETE;环境雌激素产品,如BPA,PGHS(也叫COX);细胞色素P450(CYPs);谷胱甘肽S-转移酶(GSTs);氧化应激;DNA损伤;非洲爪蟾蜍;药物代谢;乳腺和前列腺微阵列;创伤修复等相关产品。

 


详情请咨询Detroit R&D全国代理-上海金畔生物科技 

Detroit R&D——14,15-EET/DHET Hypertension ELISA Kit

Detroit R&D——14,15-EET/DHET Hypertension ELISA Kit

货号DH2

名称:14,15-EET/DHET Hypertension ELISA Kit

规格:1 kit

产品描述:

用于14,15-EET/DHET测量的DH2与用于14,15-DHET测量的DH1相同。与DH2相比,与DH1的唯一区别是样品制备步骤(而不是Elisa试剂盒),其中EET被化学改为DHET

检测样本:cellsurine

保存与稳定性This kit will obtain optimal results if all of the components are stored at the proper temperature prior to use.  Items should be stored at the designated temperatures upon receipt of this kit.

All components are stored below -20°C and should not be re-frozen and thawed more than necessary

试剂盒组成:

14,15-DHET ELISA Plate   Solid 96-well plate coated with anti-14,15-DHET antibody in each well

14,15-DHET Standard (2 μL)           Stock standard at a concentration of 1 mg/mL

14,15-DHET-HRP Conjugates (12 μL)          1000 X concentrated solution

Sample Dilution Buffer (25 mL)      10 X solution of Tris-buffered saline with preservatives

HRP Buffer (15 mL)                 1 X solution of Tris-buffered saline with preservatives

Wash Buffer Solution(25mL)        10 X solution of Tris-buffered saline with detergents and preservatives

TMB Substrate (24 mL)                A solution of TMB (tetra methyl benzadine)

 

关于Detroit R&D

 

Detroit R&D公司由Dr. Hyesook Kim创立于1997年,Detroit R&D通过领先的技术提供优质产品,致力于赢得科研工作者的绝对信任。Detroit R&D的特色产品涉及环境健康科学、高血压、乳腺癌和前列腺癌相关研究领域,包括类花生酸生物活性研究相关产品,如EET,DHET20-HETE;环境雌激素产品,如BPAPGHS(也叫COX);细胞色素P450CYPs;谷胱甘肽S-转移酶(GSTs);氧化应激;DNA损伤;非洲爪蟾蜍;药物代谢;乳腺和前列腺微阵列;创伤修复等相关产品。

详情请咨询Detroit R&D全国代理-上海金畔生物科技