标签归档：derived

肝细胞Human Stem Cell Derived Hepatocytes酶试剂盒Takara Clontech

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肝细胞Human Stem Cell Derived Hepatocytes
品牌 Code No. 产品名称 包装量 价格（元） 说明书 数量
Cellartis Y10133 Cellartis® Enhanced hiPS-HEP v2 (from ChiPSC12) Kit 1 kit 询价 肝细胞Human Stem Cell Derived Hepatocytes 肝细胞Human Stem Cell Derived Hepatocytes 肝细胞Human Stem Cell Derived Hepatocytes
Cellartis Y10134 Cellartis® Enhanced hiPS-HEP v2 (from ChiPSC18) Kit 1 kit 询价 肝细胞Human Stem Cell Derived Hepatocytes 肝细胞Human Stem Cell Derived Hepatocytes 肝细胞Human Stem Cell Derived Hepatocytes
Cellartis Y10135 Cellartis® Enhanced hiPS-HEP v2 (from ChiPSC22) Kit 1 kit 询价 肝细胞Human Stem Cell Derived Hepatocytes 肝细胞Human Stem Cell Derived Hepatocytes 肝细胞Human Stem Cell Derived Hepatocytes
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人iPS干细胞分化来的肝细胞
Cellartis enhanced hiPS-HEP v2 kits 提供了可持续供应的、成熟的人诱导多能干细胞（hiPS）来源的肝脏细胞。在超过14天的检测窗口期，hiPS-HEP细胞拥有功能性的药物代谢机制，使其成为体外药物研发、药物代谢、和相关毒性研究的理想选择；具备成熟、稳健的代谢特征，支持其作为代谢疾病模型。方便使用的试剂盒包括完全的培养基和组份，用于长期检测。与其他肝脏细胞模型相比，培养的Cellartis hiPS-HEP细胞存活时间更长，进而拥有更长的检测窗口用于慢性-毒性实验和作为代谢疾病模型研究。
 
■ 产品特点
· Complete kit for thawing, plating, and culturing the provided cells
· Highly homogeneous population with consistent performance between batches and over time
· Cells are functional and stable for a 14-day assay window
· Cells show long-term, stable CYP450 activities
· Cells express key hepatocyte proteins, including α1AT, Alb, CK18, HNF4α, and E-cadherin
· Cells secrete physiologically relevant levels of albumin and urea
· Cells demonstrate functional insulin and glucose regulation
· Cells show phase I and II enzyme activities
· Cells demonstrate LDL uptake
 
■ 产品组成
· 1 vial of ～1.2 × 107 cells
· Hepatocyte thawing medium
· Hepatocyte coating
· Hepatocyte plating medium
· Hepatocyte washing medium
· Hepatocyte long-term maintenance medium
 
■ 产品应用
· Toxicity testing, including chronic toxicity assays
· Drug discovery and development
· Metabolic studies
· Metabolic disease modeling
 
■ 产品详情请点击：肝细胞Human Stem Cell Derived Hepatocytes
 
肝细胞Human Stem Cell Derived Hepatocytes
 
Cellartis 人iPS细胞来源的肝脏细胞可以作为长期的、可靠的肝脏疾病模型。
Cellartis human induced pluripotent stem (iPS) cell-derived hepatocytes are a long-lasting, reliable liver disease model. Ideal for long-term studies, these mature, functional, pure hepatocytes allow you to generate consistent results with low batch-to-batch variability. Cellartis iPSC-derived hepatocytes can be used for extended culture significantly longer than human primary hepatocytes—allowing you to get more data from your chronic toxicity studies.
 
肝细胞Human Stem Cell Derived Hepatocytes
 
Cellartis enhanced hiPS-HEP cells可以稳定表达CYP450 活性超过21天。
CYP450 activity is stable in enhanced hiPS-HEP cells over 21 days. LC/MS was used to analyze CYP450 activity in cultured enhanced hiPS-HEP cells previously derived from the hiPS cell lines ChiPSC12, ChiPSC18, and ChiPSC22 (abbreviated as C12, C18, and C22). CYP3A (Panel A), CYP2C9 (Panel B), CYP1A (Panel C), and CYP2C19 (Panel D) activities in enhanced hiPS-HEP cells are stable over an extended culture time. Cryopreserved human primary hepatocytes (hphep), which are functional in culture for a significantly shorter time than enhanced hiPS-HEP cells, were thawed and cultured for 20 hours, and their data are shown as the black bar in each panel.
 
肝细胞Human Stem Cell Derived Hepatocytes
 
Cellartis enhanced hiPS-HEP细胞在20天的培养中表达白蛋白（Albumin）。
Albumin is present in enhanced hiPS-HEP cells through 20 days in culture. Panel A. Representative images of enhanced hiPS-HEP cells from human iPS cell line ChiPSC18 (C18) taken 12 days after thawing (right), compared to cryopreserved human primary hepatocytes (hphep) taken 24 hr after thawing (left). Cells were stained for albumin and DAPI. Panel B. Albumin secretion as measured by ELISA; n=2 for enhanced hiPS-HEP cells, with the exception of C18 at 20 days (n=1), and n=3 donors for hphep.
 
（以上图片均来源于Takara Bio USA, Inc.）
 
参考文献：
1. Heins et al. Stem Cells 2004; 22: 367-376.United States National Stem Cell Bank; http://www.nationalstemcellbank.org.
2. Mantel N et al. Potential markers of attenuation of YF virus after infection of stem cell-derived human hepatocytes with wild-type Asibi or live-attenuated YF17D virus.Supplement to The American Journal of tropical Medicine and Hygiene, Volume 83, November 2010, Number 5, abstract 12.
3. Yildirimman R et al. Human embryonic stem cell derived hepatocyte-like cells as a tool for in vitro hazard assessment of chemical carcinogenicity. Toxicol. Sci. 2011 Dec; 124(2): 278-90.
4. Ulvestad M et al. Drug metabolizing enzyme and transporter protein profi les of hepatocytes derived from human embryonic and induced pluripotent stem cells. Biochem Pharmacol. 2013 Sep 1; 86(5):691-702.
 
 

页面更新：2024-02-29 10:53:51

内胚层干细胞Human Stem Cell Derived Definitive Endoderm Cells酶试剂盒Takara Clontech

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内胚层干细胞Human Stem Cell Derived Definitive Endoderm Cells
品牌 Code No. 产品名称 包装量 价格（元） 说明书 数量
Cellartis Y10040 Cellartis® Definitive Endoderm Cells (from ChiPSC18) 1 Vial 询价 内胚层干细胞Human Stem Cell Derived Definitive Endoderm Cells 内胚层干细胞Human Stem Cell Derived Definitive Endoderm Cells 内胚层干细胞Human Stem Cell Derived Definitive Endoderm Cells
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Cellartis定型内胚层干细胞
[Cellartis Definitive Endoderm Cells (from ChiPSC18)]
Cellartis定型内胚层干细胞（DE cell）是从hiPS细胞株ChiPSC18经体外诱导分化得来。该产品中，大于90%的细胞显示表达SOX17蛋白。该产品为低温冷冻保存的细胞悬浮形式。本产品适合用于进一步诱导分化成为肝脏细胞类型或是胰脏细胞类型。特别地，Cellartis的肝脏细胞诱导分化试剂盒（Cellartis Hepatocyte Differentiation Kit，Cat. No. Y30050）适合用于DE向肝脏细胞类型的诱导分化工作。
 
■ 产品特点
· Definitive endoderm cells with >90% SOX17-positive cells
· Can be used for generating further-specified cells of endodermal origin, such as hepatocytes or pancreatic endoderm
 
■ 关联产品
Code No. Product Size
Y10052 Cellartis® HEP Coat 3 ml
Y30012 Cellartis® DEF-CS™ 500 COAT-1 4 ml
T303 iMatrix-511 2 × 175 μg
T304 iMatrix-511 6 × 175 μg
Y30050 Cellartis® Hepatocyte Differentiation Kit 1 Kit
 
■ 产品详情请点击：内胚层干细胞Human Stem Cell Derived Definitive Endoderm Cells
 
内胚层干细胞Human Stem Cell Derived Definitive Endoderm Cells
Immunocytochemistry of Cellartis Definitive Endoderm Cells (from ChiPSC18). Cells were thawed, cultured for two days, fixed, and stained for the pluripotency marker OCT4, the definitive endoderm marker SOX17, and the nuclear marker DAPI. Results show that more than 90% of the cell population are definitive endoderm cells and have lost pluripotency.
 
 
 

页面更新：2024-02-29 10:54:51

(重组人TGF-β1)Recombinant Human TGF-β1 (HEK293 derived)

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(重组人TGF-β1)Recombinant Human TGF-β1 (HEK293 derived)

品牌：Peprotech

产品编号：100-21

规格：1mg，500µg，250µg，100µg，50µg，10µg，2µg

产品描述：

TGF-β的有三种亚型，TGF-β1、β2和β3，通过相同的受体发出信号，引起相似的生物学反应。它们是调节细胞增殖、生长、分化和运动以及细胞外基质的合成和沉积的多功能细胞因子。它们参与各种生理过程，包括胚胎发生、组织重塑和伤口愈合，主要以潜伏复合物的形式被分泌，储存在细胞表面和细胞外基质中。TGF-β1最初被鉴定为具有诱导成纤维细胞表型转化的能力，最近有研究者认为它与皮肤肿瘤的形成有关。重组人TGF-β1来源于HEK293细胞，是一个分子量为25.0 kDa的蛋白，每个亚基包含112个氨基酸残基，由单个二硫键连接。

来源：HEK293 cells

别名：Transforming Growth Factor-β1, Differentiation inhibiting factor, Cartilage-inducing factor

氨基酸序列：

ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS

纯度：≥ 98% 

生物活性：通过TGF-beta1抑制il -4依赖性小鼠HT-2细胞增殖的能力测定ED50≤0.05 ng/ml，对应的比活性≥2 × 107单位/mg。

分子量：25 kDa

Accession Number: P01137

Gene ID:7040

交叉反应种属：鸡，牛，狗，驴，青蛙，人，水貂，猴，小鼠，小鼠+病毒，猪，兔，大鼠

原产地：美国

注：不供人类使用

更多详情请咨询Peprotech代理-上海金畔生物

小肠上皮细胞Human Stem Cell Derived Intestinal Epithelial Cells酶试剂盒Takara Clontech

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小肠上皮细胞Human Stem Cell Derived Intestinal Epithelial Cells
品牌 Code No. 产品名称 包装量 价格（元） 说明书 数量
Cellartis Y50035 Cellartis® Intestinal Epithelial Cells (from ChiPSC18) Kit 1 Kit ￥23,991 小肠上皮细胞Human Stem Cell Derived Intestinal Epithelial Cells 小肠上皮细胞Human Stem Cell Derived Intestinal Epithelial Cells 小肠上皮细胞Human Stem Cell Derived Intestinal Epithelial Cells
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来源于人类iPS细胞的小肠上皮细胞
(Intestinal Epithelial Cells Derived from Human Induced Pluripotent Stem Cells)
Cellartis Intestinal Epithelial Cells (from ChiPSC18) 是来源于人iPS细胞的小肠上皮细胞，具备肠上皮样特征，包括形成紧密连接、表达绒毛蛋白villin和肠转录因子CDX2。与人结肠癌Caco-2细胞系相比，该细胞具有更高的代谢酶CYP3A4和转运蛋白PEPT1的表达，为药物代谢吸收、药物相互作用评估提供更好的预测结果。
Cellartis Intestinal Epithelial Cells (from ChiPSC18) Kit 包括1管冻存的细胞Cellartis Intestinal Epithelial Cells (from ChiPSC18)和1个试剂盒Cellartis IEC Maturation Kit。解冻的Cellartis Intestinal Epithelial Cells (from ChiPSC18)使用Cellartis IEC Maturation Kit预先培养5天后，可用于各种检测。
 
■ 产品特点
· 解冻5天内细胞形成紧密连接，解冻5-8天后可用于检测
· 细胞高表达肠上皮标志物 (villin、CDX2等)
· 与Caco-2细胞相比，拥有更高的代谢酶CYP3A4和转运蛋白PEPT1的表达
· 细胞形成一个功能性的、可渗透的屏障，可用于预测药物在体内的渗透性和吸收
 
■ 产品应用
· 药物在小肠的吸收和代谢评价
· 药物研发中的ADME分析
· 药物渗透性检测
 
■ 产品组成
· 1 vial of～4.8 x 106 cells
· 1 Cellartis IEC Maturation Kit（IEC Maturation Basal Medium；IEC Supplement A；IEC Supplement B；IEC Supplement C；IEC Supplement D）
 
小肠上皮细胞Human Stem Cell Derived Intestinal Epithelial Cells
 
实验例
小肠上皮细胞Human Stem Cell Derived Intestinal Epithelial Cells
图1. 紧密连接蛋白ZO-1的免疫荧光染色结果显示，解冻后第5天，hiPSC-derived intestinal epithelial cells (IEC)细胞间形成紧密连接（如闭锁小带zonula occludens）。
 
小肠上皮细胞Human Stem Cell Derived Intestinal Epithelial Cells
图2. 在解冻后第7天，与Caco-2细胞相比，hiPSC-derived intestinal epithelial cells (IEC)中高表达肠上皮标志物villin和CDX2。
 
小肠上皮细胞Human Stem Cell Derived Intestinal Epithelial Cells
图3. 在解冻后第7天，与Caco-2细胞相比，hiPSC-derived intestinal epithelial cells (IEC)中高表达药物代谢酶CYP3A4和药物转运蛋白PEPT1。
 
 
■ 产品详情请点击：小肠上皮细胞Human Stem Cell Derived Intestinal Epithelial Cells
 
 

页面更新：2024-02-29 10:53:34

胰腺β细胞Human Stem Cell Derived Beta Cells酶试剂盒Takara Clontech

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胰腺β细胞Human Stem Cell Derived Beta Cells
品牌 Code No. 产品名称 包装量 价格（元） 说明书 数量
Cellartis Y10100 Cellartis® hiPS Beta Cells (from ChiPSC12) Kit 1 Kit 询价 胰腺β细胞Human Stem Cell Derived Beta Cells 胰腺β细胞Human Stem Cell Derived Beta Cells 胰腺β细胞Human Stem Cell Derived Beta Cells
Cellartis Y10106 Cellartis® hiPS Beta Cells (from ChiPSC22) Kit 1 Kit ￥25,928 胰腺β细胞Human Stem Cell Derived Beta Cells 胰腺β细胞Human Stem Cell Derived Beta Cells 胰腺β细胞Human Stem Cell Derived Beta Cells
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来源于人类iPS细胞的胰腺β细胞
(Pancreatic Beta Cells Derived from Human Induced Pluripotent Stem Cells)
Cellartis® hiPS Beta Cells Kit是由hiPS诱导而来的、通过体外标准化的模拟胚胎发育获得的β细胞。这些细胞分泌大量的胰岛素和C-肽，并且大量表达胰岛素/C-肽、 MAFA、NKX6.1、PDX1、UCN3等蛋白质。完整的试剂盒可以作为理想的实验工具，应用于研究β细胞功能、糖尿病/疾病模型构建、以及胰岛素分泌和调节的化合物筛选。
Cellartis®β细胞是由我们的人iPS细胞系（ChiPSC12；ChiPSC22）分化而来，制备成单细胞悬液，冻存在管中。该试剂盒含有β细胞，包被涂层，基础培养基，以及补充剂。目前提供两种试剂盒供选择，每个试剂盒中的β细胞都来自健康人供体，其中Cellartis hiPS Beta Cells (from ChiPSC22) Kit（Code No. Y10106）的供体细胞是对糖尿病易感的HLA(human leukocyte antigen人类白细胞抗原)类型，HLA-A*02:01。
 
■ 产品特点
· Beta cells from ChiPSC12 and ChiPSC22 originate from healthy human donors
· Beta cells from ChiPSC22 originate from a donor with a diabetes-susceptible HLA type, HLA-A*02:01 (Marron et al. 2002)
· Beta cells from ChiPSC22 can be used alone or in combination with beta cells from ChiPSC12, which have an alternate HLA type
· Cells show protein expression of insulin/C-peptide, MAFA, NKX6-1, PDX1, and UCN3
· Cells demonstrate insulin and C-peptide secretion
· Cells were derived with a protocol that mimics embryonic development
· Cells provide reproducible and biologically relevant data
 
■ 更多信息
Donor information
The tissues used for the reprogramming of somatic cells into hiPSCs are from donors who have signed informed consent which outlines in detail the purpose of the donation and the procedure for processing of the donated tissue. In order to protect the privacy and the confidentiality of the donors, all identifiers associated with the donors have been removed. The donor consent was obtained for commercial use. The donation did not result in any financial gain for the donors.
· ChiPSC12 was sourced from skin fibroblasts from a healthy 24-year-old European/North African male volunteer (77 kg/177 cm).
· ChiPSC22 was sourced from skin fibroblasts from a healthy 32-year-old European/North African male volunteer (74 kg/179 cm) with a diabetes-susceptible HLA type, HLA-A*02:01.
 
HLA typification data for each line is shown below.
胰腺β细胞Human Stem Cell Derived Beta Cells
 
■ 产品应用
· Disease modeling for diabetes
· Compound screening for insulin secretion and regulation
· Beta-cell functionality
· GSIS (glucose-stimulated insulin secretion) analysis
· Incretin response studies
· Autoimmune beta-cell destruction
· Pancreatitis and beta-cell function
· Transdifferentiation
 
■ 产品组成
· 1 vial (4.8 x 106 viable cells) of Cellartis hiPS Beta Cells (from ChiPSC12) or ChiPSC22
· 11 vials (360 μl/vial) of Cellartis Beta Cell Supplement
· 1 tube (4 ml) of Cellartis Beta Cell Coating
· 1 bottle (160 ml) of Cellartis Beta Cell Basal Medium
· 1 bottle (30 ml) of Cellartis Beta Cell Basal Medium 2
 
 
[Cellartis hiPS Beta Cells (from ChiPSC12) 的胰岛素和 C-肽免疫荧光染色]
胰腺β细胞Human Stem Cell Derived Beta Cells
解冻后两周的β细胞进行成熟标志胰岛素(A图) 和 C-肽 (B图)免疫荧光染色。合并数据显示了胰岛素和 C-肽共表达细胞 (C图)。细胞核使用DAPI 染色(D图)。
  （图片来源于Takara Bio USA, Inc.）
 
■ 技术资料
胰腺β细胞Human Stem Cell Derived Beta Cells
Cellartis human iPS cell-derived beta cells for diabetes drug discovery and disease modeling
Studying diabetes and metabolic disorders using hiPSC-derived beta cells.
 
■ 参考文献
Marron, M. et al. Functional evidence for the mediation of diabetogenic T cell responses by HLA-A2.1 MHC class I molecules through transgenic expression in NOD mice. Proc Nat Acad Sci U. S. A. 99, 13753–8 (2002).
 
 
 
■ 产品详情请点击：胰腺β细胞Human Stem Cell Derived Beta Cells
 
 
 

页面更新：2024-02-29 10:53:42