DNA Damage (8-OHdG) ELISA Kit

DNA Damage (8-OHdG) ELISA Kit别名有8-OH-dG ELISA Kit, 8OHG ELISA Kit, 80G ELISA Kit, 8 hydroxyguanine ELISA Kit, 8-OHdG ELISA Kit, DNA Damage ELISA Kit，下面是对这款产品的详细介绍，如对本产品有不清楚的地方，欢迎咨询Stressmarq中国代理商上海金畔生物。

研究领域：Cancer, Cell Signaling, DNA Damage and Repair, DNA/RNA, Epigenetics and Nuclear Signaling, Oxidation, Oxidative Stress, Post-translational Modifications

科学背景：8-hydroxy-2-deoxy Guanosine (8-OH-dG) is produced by the oxidative damage of DNA by reactive oxygen and nitrogen species and serves as an established marker of oxidative stress (1-4). Hydroxylation of guanosine occurs in response to both normal metabolic processes and a variety of environmental factors (i.e., anything that increases reactive oxygen and nitrogen species). Increased levels of 8-OH-dG are associated with the aging process as well as with a number of pathological conditions including cancer, diabetes, and hypertension(5-9). In complex samples such as plasma, cell lysates, and tissues, 8-OH-dG can exist as either the free nucleoside or incorporated in DNA. Once the blood enters the kidney, free 8-OH-dG is readily filtered into the urine, while larger DNA fragments remain in the bloodstream. Because of the complexity of plasma samples, urine is a more suitable matrix for the measurement of free 8-OH-dG than plasma. Urinary levels of 8-OH-dG range between 2.7-13 ng/mg creatine, while plasma levels of free 8-OH-dG have been reported to be between 4-21 pg/ml as determined by LC-MS (10-11).

-产品信息

产品名称：DNA Damage (8-OHdG) ELISA Kit

产品货号：SKT-120

描述：Colorimetric detection of 8-hydroxy-2-deoxy Guanosine

种属反应：Species Independent

Platform：Microplate

样品类型：Cell lysates, Plasma, Sample matrices, Urine

检测方法：Colorimetric Assay

检测类型：Competitive ELISA (Enzyme-linked Immunosorbent Assay)

用途：ELISA Kit for 8-OHdG detection in samples.

灵敏度：0.59 ng/mL

分析范围：0.94 – 60 ng/mL

精密度：Intra-Assay Precision: Three samples of known concentration were assayed thirty times on one plate; the intra-assay coefficient of variation of the DNA Damage ELISA has been determined to be<5%. Inter-Assay Precision: Three samples of known concentration were assayed thirty times in three individual assays; the inter-assay coefficient of variation of the DNA Damage ELISA has been determined to be <5%.

孵育时间：1 hour

样本数目：39 samples in duplicate

应用领域：Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

-产品属性

储藏温度：4ºC and -20ºC

运输温度：Blue Ice

产品类型：ELISAKits

概述：1. Prepare standard and samples in the Sample and Standard Diluent. 2. Add 50 µL of prepared standards and samples in triplicate to appropriate wells. 3. Add 50 µL of the diluted antibody preparation to the appropriate wells. 4. Cover plate with Plate Cover and incubate at room temperature (20-25°C) for 1 hour. 5. Wash plate 4 times with 1X Wash Buffer. 6. Add 100 µL of TMB Substrate to each well. 7. Cover plate and develop the plate in the dark at room temperature for 30 minutes. 8. Add 100 µL of Stop Solution to each well. 9. Measure absorbance on a plate reader at 450 nm. 10. Plot the standard curve and calculate sample concentrations.

文献参考：

1. Maxey K.M., Maddipati K.R., Birkmeier J. (1992) J Clin Immunoassay 15: 116-120.

2. Pradelles P., Grassi J., Maclouf J. (1990) Methods Enzymol. 187: 24-34.

3. Maclouf J., Grassi J., Pradelles P. (1987) Dev Immunoassay Tech Meas eicosanoids.

4. Lin H., et al. (2004) Biochem J. 380: 541-548.

5. Bogdanov M.B., et al. (1999) Free Radic Biol Med. 27(5/6): 647-666.

6. Lee J., et al. (2005) Hypertension 45: 986-990.

7. Leinonen, J., et al. (1997) FEBSLett. 417: 150-152.

8. Endo K., et al. (2006) J. Atheroscler. Thromb. 13:68-75.

9. Kuo H., et al. (2007) Mutat Res. 631:62-68.

10. Shen J., et al. (2007) Cancer 109: 574-580.

11. Beckman K.B., Ames B.N. (1997) J Biol Chem 272: 19633-19636.

12. Epe B., et al. (1996) Nucleic Acids Res 24: 4105-4110.

13. Spencer J.P.E., et al. (1995) FEBS Lett 374: 233-236.

14. Floyd R.A. (1990) FASEB J 4: 2587-2597.

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