NADPH-细胞色素C还原酶(NCR)活性检测试剂盒,Micro NADPH-cytochrome C Reductase Assay Kit,货号：BC2725- 100管/96样
| 市场价： | ￥1980.0 | |
| 价格： |
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| 品牌： | solarbio | |
| 规格： | 100管/96样 |
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说明书下载
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标签归档：cytochrome
Recombinant human CYCS protein
Recombinant human CYCS protein
货号：
P02347
品牌：
Jinpan
产品简介
| 基因名 | CYCS |
| 英文全称 | Cytochrome C |
| 别称 | CYC, CYCS, Cytochrome c, cytochrome c, somatic, HCS, THC4 |
| 中文名称 | 细胞色素C |
| Uniprot | P99999 |
| GeneID | 54205 |
| 交叉反应(推测) | Human Mouse Rat (Bovine Horse Pig Rabbit Dog Sheep Gorilla Gorilla) |
| 氨基酸大小 | 12kDa |
| 表达氨基酸 | 1-105aa |
| Background | Ribosomes, the complexes that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit.Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins.This gene encodes a ribosomal protein that is a component of the 60S subunit.The protein belongs to the L3P family of ribosomal proteins and it is located in the cytoplasm.The protein can bind to the HIV-1 TAR mRNA, and it has been suggested that the protein contributes to tat-mediated transactivation.This gene is co-transcribed with several small nucleolar RNA genes, which are located in several of this gene’s introns.Alternate transcriptional splice variants, encoding different isoforms, have been characterized.As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. |
| Application | Immunology research |
| Derived from | E.coli |
| Purification | NI-NTA affinity purification |
| Formulation | Lyophilized from a 0.2 μm filtered solution of Tris-HCl, PH8.0. |
| Shipping | The product is shipped at -20°C temperature. Upon receipt, store it immediately at the temperature listed below. |
| Storage | Reconstituted protein solution can be stored at 4-7°C for 1-2 months, stable at < -20°C for 2-3 years. |
| Reconstitution | Reconstituted protein solution can be diluted with distilled PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Purity | Purity≥85% as determined by reducing SDS-PAGE. |
| Bioactive | Unknown. (Ban’t be used in biological activity experiments such as cell culture, treatment and diagnosis.) |
| 规格 | 10ug 50ug 100ug 1mg |
Rabbit anti-Cytochrome b245 Light Chain Polyclonal Antibody
Rabbit anti-Cytochrome b245 Light Chain Polyclonal Antibody别名宿主特异性反应种属预测反应种属应用分子量免疫原浓度纯化方法类型同种型储存/保存方法存储溶液研究领域背景说明细胞定位UniProt
| 概述 | |
| 别名 |
Cytochrome b245 Light Chain抗体;CYBA; Cytochrome b 245 alpha polypeptide; Cytochrome b 245 light chain; Cytochrome b alpha polypeptide; Cytochrome b(558) alpha chain; Cytochrome b(558) alpha subunit; Cytochrome b558 subunit alpha; Flavocytochrome b 558 alpha polypeptide; Neutrophil cytochrome b 22 kDa polypeptide; p22 phagocyte B-cytochrome; CY24A_HUMAN; p22-phox; p22phox; Superoxide-generating NADPH oxidase light chain subunit; p22-PHOX
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| 宿主 |
Rabbit
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| 特异性 |
Cytochrome b245 Light Chain
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| 反应种属 |
Human, Mouse
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| 预测反应种属 |
Rat;
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| 应用 |
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
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| 分子量 |
Predicted molecular weight: 21kDa
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 免疫原 |
KLH conjugated synthetic peptide derived from human CK18 Cytochrome b245 Light Chain/p22phox:21-120/195
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| 性能 | |
| 浓度 |
1mg/ml
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| 纯化方法 |
affinity purified by Protein A
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| 类型 |
Polyclonal Antibody
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| 同种型 |
IgG
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| 储存/保存方法 |
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
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| 存储溶液 |
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
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| 研究领域 |
肿瘤 细胞生物 免疫学 淋巴细胞 t-淋巴细胞 新陈代谢
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| 靶标 | |
| 背景说明 |
Mox1 and the glycoprotein gp91-phox are largely related proteins that are essential components of the NADPH oxidase. The superoxide-generating NADPH oxidase is present in phagocytes, neuroepithelial bodies, vascular smooth muscle cells and endothelial cells. It includes a membrane-bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane, where they associate with the flavocytochrome cytochrome b558 to form the active enzyme complex. The p22- and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions.
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| 细胞定位 |
细胞膜
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| UniProt |
P13498
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实验结果图
Tissue/cell: human liver carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min;
Incubation: Anti-CYBA Polyclonal Antibody, Unconjugated secondary primary antibody 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
The blue histogram is unstained cells(mouse Splenocytes). The green histogram is cells stained with Rabbit Anti-Cytochrome b245 Light Chain antibody plus secondary antibody. Concentration 1:50.
细胞色素b5含量检测试剂盒 微量法,Micro Cytochrome b5 Assay Kit,货号：BC2765- 100管/96样
细胞色素b5含量检测试剂盒 微量法,Micro Cytochrome b5 Assay Kit,货号：BC2765- 100管/96样
| 市场价： | ￥990.0 | |
| 价格： |
|
|
| 品牌： | solarbio | |
| 规格： | 100管/96样 |
|
产品详情
说明书下载
参考文献
|