Anti-CD252 Reference Antibody(Oxelumab)

Anti-CD252 Reference Antibody(Oxelumab)描述别名宿主反应种属应用分子量纯化方法类型同种型储存/保存方法背景说明

概述
描述
内毒素:<1 eu>纯度:>95%
别名
TNFSF4; OX40L
宿主
CHO
反应种属
Human
应用
ELISA, Kinetics (BLI), Kinetics (SPR), Flow Cyt, Functional assay
分子量
145.5 kDa
性能
纯化方法
Protein A purified
类型
Monoclonal Antibody
同种型
IgG
储存/保存方法
Store at -80℃ for one year.
靶标
背景说明
Anti-CD252 Reference Antibody(Oxelumab) is expressed from CHO. The heavy chain type is huIgG1, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa.

Rabbit anti-CD38 Recombinant Monoclonal Antibody(290-45)

Rabbit anti-CD38 Recombinant Monoclonal Antibody(290-45)别名宿主反应种属应用免疫原形式浓度纯化方法类型克隆号储存/保存方法存储溶液背景说明细胞定位UniProt

概述
别名
2′-phospho-ADP-ribosyl cyclase; 2′-phospho-cyclic-ADP-ribose transferase; ADPRC 1; cADPR hydrolase 1; I-19; NIM-R5 antigen
宿主
Rabbit
反应种属
Mouse
应用
WB: 1:1000, IHC-P: 1:1600
免疫原
Recombinant protein
性能
形式
Liquid
浓度
0.05 mg/ml
纯化方法
Protein A affinity column
类型
Monoclonal Antibody
克隆号
290-45
储存/保存方法
Store at -20℃ for one year.
存储溶液
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
靶标
背景说明
CD38 (cluster of differentiation 38), also known as cyclic ADP ribose hydrolase is a glycoprotein found on the surface of many immune cells (white blood cells), including CD4+, CD8+, B lymphocytes and natural killer cells. CD38 functions in cell adhesion, signal transduction and calcium signaling. The loss of CD38 function is associated with impaired immune responses, metabolic disturbances, and behavioral modifications including social amnesia possibly related to autism. The CD38 protein is a marker of cell activation. It has been connected to HIV infection, leukemias, myelomas, solid tumors, type II diabetes mellitus and bone metabolism, as well as some genetically determined conditions.
细胞定位
Membrane
UniProt
P56528

实验结果图

Rabbit anti-CD38 Recombinant Monoclonal Antibody(290-45)

WB result of CD38 Rabbit mAb Primary antibody: CD38 Rabbit mAb at 1/1000 dilution Lane 1: mouse spleen lysate 20 µg Secondary antibody: #JP20040 at 1/10000 dilution Predicted MW: 34 kDa Observed MW: 39 kDa Exposure time: 180s

Rabbit anti-CD38 Recombinant Monoclonal Antibody(290-45)

WB result of CD38 Rabbit mAb Primary antibody: CD38 Rabbit mAb at 1/1000 dilution Lane 1: mouse colon lysate 20 µg Secondary antibody: #JP20040 at 1/10000 dilution Predicted MW: 34 kDa Observed MW: 39 kDa Exposure time: 60s

Rabbit anti-CD38 Recombinant Monoclonal Antibody(290-45)

IHC shows positive staining in paraffin-embedded mouse liver. Anti-CD38 antibody was used at 1/1600 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Rabbit anti-CD38 Recombinant Monoclonal Antibody(290-45)

IHC shows positive staining in paraffin-embedded mouse cardiac muscle. Anti-CD38 antibody was used at 1/1600 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Rabbit anti-CD38 Recombinant Monoclonal Antibody(290-45)

IHC shows positive staining in paraffin-embedded mouse colon. Anti-CD38 antibody was used at 1/1600 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Rabbit anti-CD38 Recombinant Monoclonal Antibody(290-45)

IHC shows positive staining in paraffin-embedded mouse stomach. Anti-CD38 antibody was used at 1/1600 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Rabbit anti-CD38 Recombinant Monoclonal Antibody(290-45)

IHC shows positive staining in paraffin-embedded mouse spleen. Anti-CD38 antibody was used at 1/1600 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Rabbit anti-CD73 Recombinant Monoclonal Antibody(218-52)

Rabbit anti-CD73 Recombinant Monoclonal Antibody(218-52)别名宿主反应种属应用免疫原形式浓度纯化方法类型克隆号储存/保存方法存储溶液背景说明细胞定位UniProt

概述
别名
5′-nucleotidase; 5′-NT; 5′-deoxynucleotidase
宿主
Rabbit
反应种属
Human, Mouse, Rat
应用
WB: 1:1000, IHC-P: 1:500-1:1000
免疫原
Synthetic peptide
性能
形式
Liquid
浓度
0.5 mg/ml
纯化方法
Protein A affinity column
类型
Monoclonal Antibody
克隆号
218-52
储存/保存方法
Store at -20℃ for one year.
存储溶液
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
靶标
背景说明
CD73, also known as ecto-5′-nucleotidase (ecto-5′-NT, EC 3.1.3.5) is a glycosylphosphatidyl inositol (GPI)-anchored cell surface protein that is encoded by NT5E gene. CD73 is widely expressed on different tissues [PubMed: 9553767, PubMed: 18404475] and cell types including, but not limited to the subsets of T cells and B cells [PubMed: 2550543], endothelial cells [PubMed: 9015312, PubMed: 15358667] and epithelial cells [PubMed: 9169488].
细胞定位
Cell membrane
UniProt
P21589

实验结果图

Rabbit anti-CD73 Recombinant Monoclonal Antibody(218-52)

WB result of CD73 Rabbit mAb Primary antibody: CD73 Rabbit mAb at 1/1000 dilution Lane 1: Raji whole cell lysate 20 µg Lane 2: HT-29 whole cell lysate 20 µg Lane 3: A431 whole cell lysate 20 µg Lane 4: HepG2 whole cell lysate 20 µg Negative control: Raji whole cell lysate Secondary antibody: #JP20040 at 1/10000 dilution Predicted MW: 63 kDa Observed MW: 70 kDa Exposure time: 120s

Rabbit anti-CD73 Recombinant Monoclonal Antibody(218-52)

WB result of CD73 Rabbit mAb Primary antibody: CD73 Rabbit mAb at 1/1000 dilution Lane 1: mouse brain lysate 20 µg Secondary antibody: #JP20040 at 1/10000 dilution Predicted MW: 63 kDa Observed MW: 68 kDa Exposure time: 180s

Rabbit anti-CD73 Recombinant Monoclonal Antibody(218-52)

WB result of CD73 Rabbit mAb Primary antibody: CD73 Rabbit mAb at 1/1000 dilution Lane 1: rat brain lysate 20 µg Secondary antibody: #JP20040 at 1/10000 dilution Predicted MW: 63 kDa Observed MW: 70 kDa Exposure time: 180s

Rabbit anti-CD73 Recombinant Monoclonal Antibody(218-52)

IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD73 antibody was used at 1/500 dilution, Secondary antibody: #JP20025. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Rabbit anti-CD73 Recombinant Monoclonal Antibody(218-52)

IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-CD73 antibody was used at 1/500 dilution, Secondary antibody: #JP20025. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Rabbit anti-CD73 Recombinant Monoclonal Antibody(218-52)

IHC shows positive staining in paraffin-embedded human liver. Anti-CD73 antibody was used at 1/1000 dilution, Secondary antibody: #JP20025. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Rabbit anti-CD73 Recombinant Monoclonal Antibody(218-52)

IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-CD73 antibody was used at 1/500 dilution, Secondary antibody: #JP20025. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Rabbit anti-CD73 Recombinant Monoclonal Antibody(218-52)

IHC shows positive staining in paraffin-embedded mouse kidney. Anti-CD73 antibody was used at 1/500 dilution, Secondary antibody: #JP20025. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Rabbit anti-CD73 Recombinant Monoclonal Antibody(218-52)

IHC shows positive staining in paraffin-embedded rat liver. Anti-CD73 antibody was used at 1/1000 dilution, Secondary antibody: #JP20025. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Anti-CD262 Reference Antibody(Conatumumab)

Anti-CD262 Reference Antibody(Conatumumab)描述别名宿主反应种属应用分子量纯化方法类型同种型储存/保存方法背景说明

概述
描述
内毒素:<1 eu>纯度:>95%
别名
TNFRSF10B; TRAILR2
宿主
CHO
反应种属
Human
应用
ELISA, Kinetics (BLI), Kinetics (SPR), Flow Cyt, Functional assay
分子量
144.96 kDa
性能
纯化方法
Protein A purified
类型
Monoclonal Antibody
同种型
IgG
储存/保存方法
Store at -80℃ for one year.
靶标
背景说明
Anti-CD262 Reference Antibody(Conatumumab) is expressed from CHO. The heavy chain type is huIgG1, and the light chain type is hukappa. It has a predicted MW of 144.96 kDa.

单克隆抗体Anti-CD3 mAb GMP grade,Anti-CD3 monoclonal antibody (Clone OKT3)酶试剂盒Takara Clontech

上海金畔生物科技有限公司代理Takara酶试剂盒全线产品,欢迎访问官网了解更多产品信息和订购。

单克隆抗体Anti-CD3 mAb GMP grade,Anti-CD3 monoclonal antibody (Clone OKT3)
品牌 Code No. 产品名称 包装量 价格(元) 说明书 数量
Takara T210 Anti-CD3 mAb GMP grade,Anti-CD3 monoclonal antibody (Clone OKT3) 1 mg/1.0 ml ¥7,138 单克隆抗体Anti-CD3 mAb GMP grade,Anti-CD3 monoclonal antibody (Clone OKT3) 单克隆抗体Anti-CD3 mAb GMP grade,Anti-CD3 monoclonal antibody (Clone OKT3) 单克隆抗体Anti-CD3 mAb GMP grade,Anti-CD3 monoclonal antibody (Clone OKT3)
收藏产品 加入购物车
 
■ 制品说明:
Anti-CD3抗体常用于T淋巴细胞增殖实验中,而“OKT3”(mouse IgG2a)又是在Anti-CD3众多克隆来源中非常常用的。 Anti-CD3单克隆抗体(clone OKT3)是依据GMP(Good Manufacturing Practice)标准制造的有质量保证的制品。本制品可用于体外细胞培养,且仅限于研究用。
 
■ 来 源
Mouse hybridoma cells
 
■ 纯 度
HPLC纯度>95%
 
■ 贮存溶液
phosphate-buffered saline (PBS), pH7.4
200 ppm polysorbate 80
 
■ 包 装
2 ml冷冻管中装有1.0 mg protein/1.0 ml。
 
 
温馨提示:关于此产品的订购和技术疑问,请致电010-80720983。
 
 

页面更新:2024-01-25 15:07:10

抗CD63单克隆抗体(3-13),荧光标记试剂盒-Wako富士胶片和光

抗CD63单克隆抗体(3-13),荧光标记试剂盒-Wako富士胶片和光

供货周期 一个月以上 应用领域 生物产业

高灵敏度抗CD63抗体
抗CD63单克隆抗体(3-13),荧光标记
本品是对用DNA免疫法建立的CD63(lysosome-associated membrane glycoprotein : LAMP3)的小鼠单克隆抗体的荧光标记物。CD63是具有4个跨膜结构域的跨膜蛋白,能用作细胞外囊泡或活性血小板的标记蛋白。

高灵敏度抗CD63抗体抗CD63单克隆抗体(3-13),荧光标记试剂盒-Wako富士胶片和光

抗CD63单克隆抗体(3-13),荧光标记

 

 

  本品是对用DNA免疫法建立的CD63(lysosome-associated membrane glycoprotein : LAMP3)的小鼠单克隆抗体的荧光标记物。CD63是具有4个跨膜结构域的跨膜蛋白,能用作细胞外囊泡或活性血小板的标记蛋白。

 

 

◆特点

 

      通过搭配使用PS Capture™ 外泌体流式试剂盒,能用于人CD63阳性细胞外囊泡的流式细胞仪检测

 

 

◆产品概要

 

● 克隆号:3–13

● 免疫动物:小鼠

● 抗体亚类:IgG1

● 交叉性:人

● 适用:流式细胞仪

 

 

◆数据

 

检测COLO201细胞表面的CD63

 

抗CD63单克隆抗体(3-13),荧光标记试剂盒-Wako富士胶片和光

 

样本(细胞)数:1×106

使用荧光素标记抗CD63抗体进行COLO201细胞悬浮液的流式细胞术。

 

结果表明,与其他公司的荧光素结合抗CD63抗体相比,本产品可以高灵敏度地检测出细胞表面的CD63。

 

COLO201细胞培养上清及人血清来源外泌体表面CD63的检测

 

抗CD63单克隆抗体(3-13),荧光标记试剂盒-Wako富士胶片和光

 

  用PS Capture™ 外泌体流式试剂盒分离COLO201细胞培养上清及人血清中的外泌体,然后用本抗体对外泌体标记蛋白进行染色。

  与其他公司的荧光标记抗CD63抗体相比,本品相对于阴性对照的荧光强度峰值的位移更大,能高灵敏度检测外泌体上的CD63。

 

 

◆产品列表

 

产品编号

产品名称

规格

包装

018-27641

抗CD63单克隆抗体(3-13),荧光标记

免疫化学用

25次

014-27643

100次

 

 

◆相关产品

 

产品编号

产品名称

规格

包装

297-79701

PS Capture™ 外泌体流式试剂盒

基因研究用

300次

 

Human B7-H2/ICOSL/CD275 CHO-K Cell Line

Human B7-H2/ICOSL/CD275 CHO-K Cell Line描述来源应用浓度使用方法储存/保存方法形态外观靶点

产品描述
描述
细胞类型:CHO-K;
载体类型:GSV0
来源
Human
应用
免疫;抗体筛选;体外功能研究
浓度
5E6 cells/vial
使用方法
复苏后培养
储存/保存方法
Liquid nitrogen. 细胞冻存:90% Medium+10% DMSO
形态
液体冻存
基本信息
外观
淡黄色
生物活性
靶点
B7-H2, ICOSL, CD275

Rabbit anti-CD3 epsilon Recombinant Monoclonal Antibody(241-49)

Rabbit anti-CD3 epsilon Recombinant Monoclonal Antibody(241-49)别名宿主反应种属应用免疫原形式浓度纯化方法类型克隆号储存/保存方法存储溶液背景说明细胞定位UniProt

概述
别名
T-cell surface antigen T3/Leu-4 epsilon chain; CD3e; T3E; CD3E
宿主
Rabbit
反应种属
Human, Mouse, Rat
应用
WB: 1:500, IP: 1:25, IHC-P: 1:2000-1:4000
免疫原
Synthetic peptide
性能
形式
Liquid
浓度
0.25 mg/ml
纯化方法
Protein A affinity column
类型
Monoclonal Antibody
克隆号
241-49
储存/保存方法
Store at -20℃ for one year.
存储溶液
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
靶标
背景说明
CD3 (cluster of differentiation 3) is a protein complex and T cell co-receptor that is involved in activating both the cytotoxic T cell (CD8+ naive T cells) and T helper cells (CD4+ naive T cells). It is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains. These chains associate with the T-cell receptor (TCR) and the CD3-zeta (ζ-chain) to generate an activation signal in T lymphocytes. The TCR, CD3-zeta, and the other CD3 molecules together constitute the TCR complex. The CD3–T cell receptor (TCR) complex plays a central role in the T-cell-mediated immunoresponse as it is involved in the recognition of antigens and subsequent signal transduction and activation of immunocompetent T lymphocytes. Because CD3 is required for T cell activation, drugs (often monoclonal antibodies) that target it are being investigated as immunosuppressant therapies (e.g., otelixizumab, teplizumab) for type 1 diabetes and other autoimmune diseases.
细胞定位
Cell membrane
UniProt
P07766

实验结果图

Rabbit anti-CD3 epsilon Recombinant Monoclonal Antibody(241-49)

WB result of CD3 epsilon Rabbit mAb Primary antibody: CD3 epsilon Rabbit mAb at 1/500 dilution Lane 1: Raji whole cell lysate 20 µg Lane 2: Jurkat whole cell lysate 20 µg Negative control: Raji whole cell lysate Secondary antibody: #JP20040 at 1/10000 dilution Predicted MW: 20 kDa Observed MW: 20 kDa Exposure time: 13s

Rabbit anti-CD3 epsilon Recombinant Monoclonal Antibody(241-49)

IHC shows positive staining in paraffin-embedded human spleen. Anti-CD3 epsilon antibody was used at 1/2000 dilution, Secondary antibody: #JP20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Rabbit anti-CD3 epsilon Recombinant Monoclonal Antibody(241-49)

IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD3 epsilon antibody was used at 1/2000 dilution, Secondary antibody: #JP20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Rabbit anti-CD3 epsilon Recombinant Monoclonal Antibody(241-49)

IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD3 epsilon antibody was used at 1/2000 dilution, Secondary antibody: #JP20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Rabbit anti-CD3 epsilon Recombinant Monoclonal Antibody(241-49)

IHC shows positive staining in paraffin-embedded human stomach. Anti-CD3 epsilon antibody was used at 1/2000 dilution, Secondary antibody: #JP20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Rabbit anti-CD3 epsilon Recombinant Monoclonal Antibody(241-49)

IHC shows positive staining in paraffin-embedded mouse spleen. Anti-CD3 epsilon antibody was used at 1/4000 dilution, Secondary antibody: #JP20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Rabbit anti-CD3 epsilon Recombinant Monoclonal Antibody(241-49)

IHC shows positive staining in paraffin-embedded rat spleen. Anti-CD3 epsilon antibody was used at 1/4000 dilution, Secondary antibody: #JP20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Rabbit anti-CD3 epsilon Recombinant Monoclonal Antibody(241-49)

ICC shows positive staining in Jurkat cells. Anti-CD3 epsilon antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG – H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).

Rabbit anti-CD3 epsilon Recombinant Monoclonal Antibody(241-49)

Negative control: ICC shows negative staining in Raji cells. Anti-CD3 epsilon antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG – H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).

Rabbit anti-CD3 epsilon Recombinant Monoclonal Antibody(241-49)

CD3 epsilon Rabbit mAb at 1/25 dilution (1µg) immunoprecipitating CD3 epsilon in 0.4mg Jurkat whole cell lysate. Western blot was performed on the immunoprecipitate using CD3 epsilon Rabbit mAb at 1/1000 dilution. Secondary antibody (HRP) for IP was used at 1/400 dilution. Lane 1 : Jurkat whole cell lysate 10µg(input) Lane 2 : CD3 epsilon Rabbit mAb IP in Jurkat whole cell lysate Lane 3 : Rabbit monoclonal IgG IP in Jurkat whole cell lysate Predicted MW: 20 kDa Observed MW: 20 kDa Exposure time: 10s

Anti-CD278 Reference Antibody(Feladilimab)

Anti-CD278 Reference Antibody(Feladilimab)描述别名宿主反应种属应用分子量纯化方法类型同种型储存/保存方法背景说明

概述
描述
内毒素:<1 eu>纯度:>95%
别名
ICOS
宿主
CHO
反应种属
Human
应用
ELISA, Kinetics (BLI), Kinetics (SPR), Flow Cyt, Functional assay
分子量
145.5 kDa
性能
纯化方法
Protein A purified
类型
Monoclonal Antibody
同种型
IgG
储存/保存方法
Store at -80℃ for one year.
靶标
背景说明
Anti-CD278 Reference Antibody(Feladilimab) is expressed from CHO. The heavy chain type is huIgG1, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa.