BioXCell热销产品InVivoMAb anti-mouse CD8α介绍

BioXCell热销产品InVivoMAb anti-mouse CD8α介绍

克隆号53-6.7单克隆抗体能与小鼠CD8α反应。CD8抗原是一种跨膜糖蛋白,作为T细胞受体的共受体。与TCR一样,CD8与抗原呈递细胞(APC)展示的IMHC分子结合。CD8主要在细胞毒性T细胞表面表达,但也可在胸腺细胞、自然杀伤细胞和一些树突状细胞亚群上发现。CD8最常见的存在形式是由一个CD8α和一个CD8β链组成的异二聚体,然而,它也可以是由两个CD8α链组成的同二聚体。CD8α和CD8β链均与免疫球蛋白可变轻链具有显著同源性。每个CD8链的分子量约为34 kDa。当在体内使用时,克隆号53-6.7抗体表现出depleting消耗活性。

 

产品详情

产品货号

BE0004-1

产品规格

1/5/25/50/100mg

抗体亚型

Rat IgG2a, κ

推荐同型对照

InVivoMAb rat IgG2a isotype control, anti-trinitrophenol(货号:BE0089

推荐抗体稀释液

InVivoPure pH 6.5 Dilution Buffer(货号:IP0065

免疫原

Mouse Spleen Cells or Thymocyte Membranes

应用

in vivo CD8+ T cell depletion

Immunofluorescence

Flow cytometry

Western blot

产品形式

PBS , pH 6.05 

Contains no stabilizers or preservatives

内毒素水平

<2eu>μ

g)

使用 LAL gel clotting 测定

纯度

>95% Determined by SDS-PAGE

无菌处理

0.2 μM filtered

生产形式

从组织培养上清液中纯化得到。

纯化形式

Protein G

RRID

AB_1107671

分子量大小

150 kDa

保存条件

抗体原溶液应保存在4°C条件下,不要冷冻保存。

 

已发表参考文献:

in vivo CD8+ T cell depletion

Christensen, A. D., et al. (2015). Depletion of regulatory T cells in a hapten-induced inflammation model results in prolonged and increased inflammation driven by T cells. Clin Exp Immunol 179(3): 485-499.

Flow cytometry

Wang, W., et al. (2018). “RIP1 Kinase Drives Macrophage-Mediated Adaptive Immune Tolerance in Pancreatic Cancer.” Cancer Cell 34(5): 757-774 e757.

Immunofluorescence

Finisguerra, V., et al. (2015). MET is required for the recruitment of anti-tumoural neutrophils. Nature 522(7556): 349-353.

详情请咨询 BioXcell 中国代理上海金畔生物科技   

清道夫受体B2抗体(Scavenger Receptor B2/CD36 Monoclonal Antibody) Item No. 10009893

清道夫受体B2抗体(Scavenger Receptor B2/CD36 Monoclonal Antibody) Item No. 10009893

本文是对Cayman热销产品清道夫受体B2抗体(Scavenger Receptor B2/CD36 Monoclonal Antibody)的产品简单介绍,该产品货号为10009893,如需购买或咨询该产品其它详情,请联系Cayman代理商上海金畔生物。


别名:GPIIIb

   GPIV

   Platelet Glycoprotein 4

   Fatty Acid Translocase

   PAS IV

   Platelet Collagen Receptor

   Thrombospondin Receptor

免疫原:Recombinant full-length mouse CD36 expressed in adenovirus

克隆号:JC63.1

缓冲液:PBS, pH 7.2

宿主:CD36 null mouse

亚型:IgA

应用:FC, ICC

 

产品介绍

 

CD36也被称为清道夫受体B2或脂肪酸转位酶,是一种跨膜糖蛋白,在脂肪酸摄取、脂质代谢和信号转导中发挥作用。它由N和C端胞质尾区,两个跨膜域和一个细胞外循环,与多种脂质结合,包括长链脂肪酸,氧化低密度脂蛋白和氧化磷脂和蛋白质配体。多种细胞可表达CD36,包括血小板、单核细胞、巨噬细胞等造血细胞,以及脂肪细胞、肠细胞、心肌和骨骼肌细胞、内皮细胞和上皮细胞。


CD36的表达受多种转录因子调控,包括过氧化物酶体增殖物激活受体(PPAR)、STAT3和HIF-1a,并以一种组织依赖的方式进行调控。Cayman的Scavenger Receptor B2/CD36单克隆抗体(Clone JC63.1)内毒素水平≤2 EU/mg可用于流式细胞术(FC)和免疫细胞(ICC)应用。

 

详细使用说明请见https://cdn.caymanchem.com/cdn/insert/10009893.pdf

 

产品信息

货号

名称

规格

10009893

Scavenger Receptor B2/CD36 Monoclonal Antibody (Clone JC63.1) (Low Endotoxin)

100μg / 500μg

 

详情请咨询 Cayman 中国代理-上海金畔生物科技

CD8 Antibody [LT8](GTX74773),20篇文献引用

CD8 Antibody [LT8](GTX74773),20篇文献引用

CD8 Antibody [LT8](GTX74773),20篇文献引用

产品名称:CD8 Antibody [LT8]

中文名称:CD8 抗体 [LT8]

货号:GTX74773

规格:200 μg

宿主:Mouse

克隆:Monoclonal

克隆编号:LT8

同种型:IgG1

实验应用:ICC/IF, IHC-P, IHC-Fr, FACS, IP, Blocking

种属反应:Human, Mouse, Monkey, Chimpanzee, Cynomolgus monkey, Marmoset, Red-bellied Tamarin

产品属性

形式:Liquid

存储溶液:PBS

存放说明:

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度:1.0 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属:Human

免疫原:Normal human peripheral blood lymphocytes.

纯化方式:Purified by ion exchange chromatography

偶联:Unconjugated

RRID:AB_376459

注意事项:仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

别名:

P37 Antibody , LYT3 Antibody , CD8B Antibody , LY3 Antibody , LEU2 Antibody , CD8b molecule Antibody , CD8B1 Antibody

适用于肿瘤标记(白血病标志物),免疫学(适应性免疫学)等研究领域。

部分文献如下:

Application Reference

Qizhi Ma et al. Precis Clin Med 2021; 4 (3) : 155-167 CXCL13 expression in mouse 4T1 breast cancer microenvironment elicits antitumor immune response by regulating immune cell infiltration.

Ma Q et al. Precision Clinical Medicine 2021; 4 (3) : 155–167 CXCL13 expression in mouse 4T1 breast cancer microenvironment elicits antitumor immune response by regulating immune cell infiltration.

实验应用 : IHC-P

种属反应 : Human

Chebloune Y et al. Vaccine 2020; 38 (21) : 3729-3739 A single lentivector DNA based immunization contains a late heterologous SIVmac251 mucosal challenge infection.

种属反应 : Mouse

Clone Reference

Kap YS et al. J Immunol 2014; 192 (9):4242-53 CD20+ B cell depletion alters T cell homing.

实验应用 : FACS

种属反应 : Monkey

Hood SP et al. Virus Res 2014; 179:93-101 Changes in immune cell populations in the periphery and liver of GBV-B-infected and convalescent tamarins (Saguinus labiatus).

更多产品详情,请咨询GeneTex代理商-上海金畔生物

Rabbit anti-CD155 Recombinant Monoclonal Antibody(289-68)

Rabbit anti-CD155 Recombinant Monoclonal Antibody(289-68)别名宿主反应种属应用免疫原形式浓度纯化方法类型克隆号储存/保存方法存储溶液背景说明细胞定位UniProt

概述
别名
PVR; Poliovirus receptor; Nectin-like protein 5; NECL-5; PVS
宿主
Rabbit
反应种属
Human
应用
WB: 1:1000, IHC-P: 1:1000-1:2000
免疫原
Recombinant protein
性能
形式
Liquid
浓度
0.25 mg/ml
纯化方法
Protein A affinity column
类型
Monoclonal Antibody
克隆号
289-68
储存/保存方法
Store at -20℃ for one year.
存储溶液
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
靶标
背景说明
Poliovirus receptor (PVR, CD155), a member of the nectin-like family of proteins, is recently emerging as a promising target in immunotherapy that enhances the existing anti-tumor responses. Similar to other members of the family, PVR is involved in important cellular processes, such as adhesion, contact inhibition, migration, proliferation, and the immune response [PMID: 18648374]. PVR is dramatically overexpressed in several human malignancies, whereas its expression is low or JPent in most healthy tissues [PMID: 10841575, PMID: 11454801, PMID: 15604290, PMID: 15536144, PMID: 19801517]. Consistent with PVR biology, its overexpression promotes tumor cell invasion, migration, and proliferation and is associated with a poor prognosis and enhanced tumor progression [PMID: 16322240, PMID: 17893876, PMID: 25743219, PMID: 25862891]. Simultaneously, PVR has a considerable immu noregulatory potential. It interacts with the (co)stimulatory receptor DNAM-1 and the inhibitory receptors TIGIT and CD96, resulting in either immune cell activation or inhibition, respec tively [PMID: 28870470].
细胞定位
Cell membrane, Secreted
UniProt
P15151

实验结果图

Rabbit anti-CD155 Recombinant Monoclonal Antibody(289-68)

WB result of CD155 Rabbit mAb Primary antibody: CD155 Rabbit mAb at 1/1000 dilution Lane 1: Raji whole cell lysate 20ug Lane 2: HUVEC whole cell lysate 20ug Lane 3: HeLa whole cell lysate 20ug Negative control: Raji whole cell lysate Secondary antibody: #JP20040 at 1/10000 dilution Predicted MW: 45kDa Observed MW: 70kDa Exposure time: 90s

Rabbit anti-CD155 Recombinant Monoclonal Antibody(289-68)

IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-CD155/PVR antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Rabbit anti-CD155 Recombinant Monoclonal Antibody(289-68)

IHC shows positive staining in paraffin-embedded human prostate. Anti-CD155/PVR antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Rabbit anti-CD155 Recombinant Monoclonal Antibody(289-68)

IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-CD155/PVR antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Rabbit anti-CD155 Recombinant Monoclonal Antibody(289-68)

IHC shows positive staining in paraffin-embedded human breast cancer. Anti-CD155/PVR antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Rabbit anti-CD205 Polyclonal Antibody

Rabbit anti-CD205 Polyclonal Antibody描述别名宿主特异性反应种属应用分子量免疫原纯化方法类型储存/保存方法UniProt

概述
描述
Rabbit polyclonal antibody to CD205
别名
CD205抗体;CD205; CLEC13B; Lymphocyte antigen 75; Ly-75; C-type lectin domain family 13 member B; DEC-205; gp200-MR6
宿主
Rabbit
特异性
Recognizes endogenous levels of CD205 protein.
反应种属
Human, Mouse, Rat
应用
WB 1:500 – 1:1000
分子量
Detects a band of approximately 205kD(Predicted molecular weight: 198kD)
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
免疫原
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD205. The exact sequence is proprietary.
性能
纯化方法
The antibody was purified by immunogen affinity chromatography.
类型
Polyclonal Antibody
储存/保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
靶标
UniProt
O60449

实验结果图

Rabbit anti-CD205 Polyclonal Antibody

Western blot analysis of CD205 expression in MCF7 (A), SP2/0 (B), H9C2 (C), mouse lymph node (D) whole cell lysates.

BioXCell热销产品—InVivoMAb anti-human CD3(货号:BE0231)

BioXCell热销产品—InVivoMAb anti-human CD3(货号:BE0231)

克隆号UCHT1 (Leu-4)(T3)单克隆抗体与人CD3ε一种属于免疫球蛋白超家族的20 kDa跨膜细胞表面蛋白反应。CD3ε是结合形成TCR复合物的五个多肽链之一。CD3ε在T淋巴细胞NK-T细胞上表达,并在不同程度上在发育中的胸腺细胞上表达。CD3TCR信号T淋巴细胞活化和抗原识别中发挥作用。通过固定化UCHT1 (Leu-4)(T3)抗体交联TCR通常用于体外激活T细胞。

(InVivoMAb anti-human CD3产品图片)


产品详情

产品货号

BE0231

产品规格

1/5/25/50/100mg

抗体亚型

Mouse IgG1, κ

推荐同型对照

InVivoMAb mouse IgG1 isotype control, unknown specificity(货号:BE0083

推荐抗体稀释液

InVivoPure pH7.0 Dilution Buffer(货号:IP0065

免疫原

Human CD3ε

应用

in vivo T cell depletion in humanized mice

ex vivo T cell inhibition for xenographs

Flow cytometry

产品形式

PBS , pH 7.0

Contains no stabilizers or preservatives

内毒素水平

<2eu>μ

g)

使用 LAL gel clotting 测定

纯度

>95% Determined by SDS-PAGE

无菌处理

0.2 μM filtered

生产形式

从组织培养上清液中纯化得到。

纯化形式

Protein G

RRID

AB_2687713

分子量大小

150 kDa

保存条件

抗体原溶液应保存在4°C条件下,不要冷冻保存。

 

已发表参考文献:

in vivo T cell depletion in humanized mice

Wunderlich, M., et al. (2014). “OKT3 prevents xenogeneic GVHD and allows reliable xenograft initiation from unfractionated human hematopoietic tissues.” Blood 123(24): e134-144.

in vivo T cell depletionn

Woo, J. H., et al. (2010). “Pharmacology of anti-CD3 diphtheria immunotoxin in CD3 positive T-cell lymphoma trials.” Methods Mol Biol 651: 157-175.

Flow cytometry

Rossi, N. E., et al. (2008). “Differential antibody binding to the surface alphabetaTCR.CD3 complex of CD4+ and CD8+ T lymphocytes is conserved in mammals and associated with differential glycosylation.” Int Immunol 20(10): 1247-1258.

 

详情请咨询 BioXcell 中国代理上海金畔生物   

Adipogen公司产品anti-CD9 (human), mAb (SN4)介绍

Adipogen公司产品anti-CD9 (human), mAb (SN4)介绍

本文是对Adipogen公司生产的产品anti-CD9 (human), mAb (SN4)进行简单的介绍,如需更全面了解anti-CD9 (human), mAb (SN4),请联系Adipogen代理商上海金畔生物。

产品名称:anti-CD9 (human), mAb (SN4)

产品描述:Human CD9 is strongly expressed on platelets, on early B cells and on activated T cells. CD9 is a member of the tetraspan family crossing the membrane four times. Both the N-terminal and C-terminal regions are on the cytoplasmic side of the plasma membrane. Studies indicate CD9 may be involved with calcium mobilization.

反应种属:Human

实验应用:FACS

同种型:Mouse IgG1kappa

免疫原:Membrane antigen preparation of NALM-6.

克隆性:Monoclonal

克隆号:SN4

保存温度:+4°C

运输温度:蓝冰

产品形式:liquid

存储溶液:50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5% Gentamicin sulfate

有效期:Stable for at least 1 year after receipt when stored at +4°C.

产地:瑞士

生产商:AdipoGen Life Sciences

样本类型:50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5% Gentamicin sulfate

订购详情:


品牌 产品名称 产品货号 规格
Adipogen anti-CD9 (human), mAb (SN4) ANC-156-020 100 ug

详情请咨询Adipogen全国代理-上海金畔生物科技 

Human CD14 ELISA KIT(人的可溶性细胞分化抗原14) ELISA KIT

Human CD14 ELISA KIT(人的可溶性细胞分化抗原14) ELISA KIT

货号:BQEH-501-96T

规格:96T

品牌:biosharp

 ELISA KIT又称为ELISA试剂盒、酶联免疫吸附测定试剂盒,是一种灵敏度高,特异性强,重复性好的实验方法,且因其试剂稳定、易保存,操作简便、结果判断客观等已广泛应用在免疫学检验的各领域中,得到全世界科研工作者的认可和应用。

ELISA的基本原理是双抗体夹心法,将样本加入预包被了抗原或抗体的酶标板里,然后再加入酶标记的抗原或抗体,从而固相载体上的抗原或抗体与样品中受检物质的量呈一定的比例。加入酶反应的底物后,底物被酶催化成为有色产物,产物的量与样本中受检物质的量直接相关,故可根据呈色的深浅进行定性或定量分析。

Jinpan ELISA KIT覆盖人、小鼠、大鼠、猪、通用等多种物种,产品达1000多种。每一个产品均经过严格的质检,结果稳定,重复性和线性范围好,特异性和灵敏度高,应用广泛,涉及免疫学、神经学、微生物学、细胞生物学、表观遗传学等多个科学领域。

货号 BQEH-501-96T
规格 96T
品牌 biosharp

Anti-CD262 Reference Antibody(Lexatumumab)

Anti-CD262 Reference Antibody(Lexatumumab)描述别名宿主反应种属应用分子量纯化方法类型同种型储存/保存方法背景说明

概述
描述
内毒素:<1 eu>纯度:>95%
别名
TNFRSF10B; TRAILR2
宿主
CHO
反应种属
Human
应用
ELISA, Kinetics (BLI), Kinetics (SPR), Flow Cyt, Functional assay
分子量
145.5 kDa
性能
纯化方法
Protein A purified
类型
Monoclonal Antibody
同种型
IgG
储存/保存方法
Store at -80℃ for one year.
靶标
背景说明
Anti-CD262 Reference Antibody(Lexatumumab) is expressed from CHO. The heavy chain type is huIgG1, and the light chain type is hulambda. It has a predicted MW of 145.5 kDa.

Anti-CD221 Reference Antibody(Ganitumab)

Anti-CD221 Reference Antibody(Ganitumab)描述别名宿主反应种属应用分子量纯化方法类型同种型储存/保存方法背景说明

概述
描述
内毒素:<1 eu>纯度:>95%
别名
IGF1R
宿主
CHO
反应种属
Human
应用
ELISA, Kinetics (BLI), Kinetics (SPR), Flow Cyt, Functional assay
分子量
145.5 kDa
性能
纯化方法
Protein A purified
类型
Monoclonal Antibody
同种型
IgG
储存/保存方法
Store at -80℃ for one year.
靶标
背景说明
Anti-CD221 Reference Antibody(Ganitumab) is expressed from CHO. The heavy chain type is huIgG1, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa.