CYTO-ID Autophagy detection kit⾃噬分析试剂盒

CYTO-ID Autophagy detection kit⾃噬分析试剂盒–⽆需转染，流式或显微镜⽅法检测

常⽤监测细胞⾃噬的⽅式是构建LC3-GFP（绿⾊荧光蛋⽩）融合蛋⽩，LC3蛋⽩可以和⾃噬体膜发⽣特异性结合，GFP作为⽰踪剂。但是构建LC3-GFP融合基因，转染，表达需要⼀定时间，⽽且LC3-GFP表达不理想也会影响细胞⾃噬的检测。该试剂盒采⽤申请专利的⾃噬荧光检测试剂，免去转染表达的时间，特异性⾼于LC3。

特点：

CYTO-ID Autophagy detection kit检测过程中⽆需转染，适合流式和显微镜⾼通量分析，CYTO-ID Autophagy detection kit组分包括，灵敏的荧光探针，可以染前⾃噬体，⾃噬⼩体，很少染上溶酶体。

专利的染⾃噬囊泡，⽆需耗时融合转染LC3

在多种细胞中快速定量⾃噬

能够忽略溶酶体的染⾊，降低了背景⼲扰

试剂盒中提供的成分：

Cyto-ID® 绿⾊检测试剂⾃噬检测试剂

Hoechst 33342 neciear stain –核染⾊

10X Assay buffer –检测缓冲液

Rapamycin –雷帕霉素，⾃噬诱导剂

Chloroquine –氯喹 ，抑制溶酶体活性，阳性诱导

结果比较：

CYTO-ID® Green dye 能够很好的与RFP-LC3 蛋⽩兼容

A CYTO-ID® Green dye

B B：RFP-LC3

C CYTO-ID® Green dye与RFP-LC3结合

利⽤不同⽅法诱导的细胞⽤CYTO-ID Autophagy detection kit 荧光显微镜检测细胞⾃噬

CYTO-ID Autophagy detection kit⽤于流式检测不同细胞⾃噬情况

产品信息：

更多详情请咨询Enzo Life Sciences代理商-上海金畔生物

CYTO-ID® Autophagy detection kit 自噬检测试剂盒

CYTO-ID® Autophagy detection kit     ENZ-51031-0050   ENZ-51031-K200

CYTO-ID自噬检测试剂盒

该试剂盒可在活细胞内定量分析细胞自噬，不需要转染。

适用于流式细胞术和显微镜。

可用于高通量筛选自噬的激活剂和抑制剂。

利用专利性的染料，可选择性的对前自噬体，自噬体和自噬性溶酶体进行染色。

文献引用

ABT-888 enhances cytotoxic effects of temozolomide independent of MGMT status in serum free cultured glioma cells: R. K. Balvers, et al.; J. Transl. Med. 13, 74 (2015), Application(s): Assay, Full Text

Bardoxolone methyl induces apoptosis and autophagy and inhibits epithelial-to-mesenchymal transition and stemness in esophageal squamous cancer cells: Y.Y. Wang, et al.; Drug Des. Devel. Ther. 9, 993 (2015), Application(s): Flow Cytometry,Abstract; Full Text

Coffee and caffeine potentiate the antiamyloidogenic activity of melatonin via inhibition of Aβ oligomerization and modulation of the Tau-mediated pathway in N2a/APP cells: L.F. Zhang, et al.; Drug Des. Devel. Ther. 9, 241 (2015), Application(s): Flow Cytometry, Abstract; Full Text

Evaluation of Antitumor Effects of Folate-Conjugated Methyl-β-cyclodextrin in Melanoma: K. Motoyama, et al.; Biol. Pharm. Bull. 38, 374 (2015), Application(s): Fluorescence Microscopy, Abstract; Full Text

Induction of apoptosis and autophagy via sirtuin1- and PI3K/Akt/mTOR-mediated pathways by plumbagin in human prostate cancer cells: Z.W. Zhou, et al.; Drug Des. Devel. Ther. 9, 1511 (2015), Application(s): Assay, Abstract; Full Text

Methicillin-Resistant Staphylococcus aureus Adaptation to Human Keratinocytes: G. Soong, et al.; MBio. 6, (2015),Application(s): Assay, Abstract; Full Text

MiR-29b replacement inhibits proteasomes and disrupts aggresome+autophagosome formation to enhance the antimyeloma benefit of bortezomib: S. Jagannathan, et al.; Leukemia 29, 727 (2015), Application(s): Detection of autophagy by fluorescence microscopy in multiple myeloma cell lines, Abstract; Full Text

Plumbagin induces G2/M arrest, apoptosis, and autophagy via p38 MAPK- and PI3K/Akt/mTOR-mediated pathways in human tongue squamous cell carcinoma cells: S. T. Pan, et al.; Drug Des. Devel. Ther. 9, 1601 (2015), Application(s): Assay,Abstract; Full Text

Pro-apoptotic and pro-autophagic effects of the Aurora kinase A inhibitor alisertib (MLN8237) on human osteosarcoma U-2 OS and MG-63 cells through the activation of mitochondria-mediated pathway and inhibition of p38 MAPK/PI3K/Akt/mTOR signaling pathway: N. K. Niu, et al.; Drug Des. Devel. Ther. 9, 1555 (2015), Application(s): Assay, Abstract; Full Text

The investigational Aurora kinase A inhibitor alisertib (MLN8237) induces cell cycle G2/M arrest, apoptosis, and autophagy via p38 MAPK and Akt/mTOR signaling pathways in human breast cancer cells: J. P. Li, et al.; Drug Des. Devel. Ther. 9, 1627 (2015), Application(s): Assay, Abstract; Full Text

The pan-inhibitor of Aurora kinases danusertib induces apoptosis and autophagy and suppresses epithelial-to-mesenchymal transition in human breast cancer cells: J.P. Li, et al.; Drug Des. Devel. Ther. 9, 1027 (2015), Application(s): Assay, Abstract;Full Text

A Novel CXCR3-B Chemokine Receptor-induced Growth-inhibitory Signal in Cancer Cells Is Mediated through the Regulation of Bach-1 Protein and Nrf2 Protein Nuclear Translocation : M. Balan & S. Pal; J. Biol. Chem 289, 3126 (2014), Application(s):Monitor autophagy in MCF-7 and T47D breast cancer cells by flow cytometry and fluorescence microscopy, Abstract;

Adaptive responses to glucose restriction enhance cell survival, antioxidant capability, and autophagy of the protozoan parasiteTrichomonas vaginalis: K. Y. Huang, et al.; Biochim. Biophys. Acta. 1840, 53 (2014), Abstract;