Glyko® 糖生物学研究工具-糖标准品

Glyko® 糖生物学研究工具-糖标准品

糖标准品

 

糖标准品(不含标记)

 

产品类型 产品名称 产品编号 规格 分子量 主要来源
N-Linked Oligosaccharides Glyko™ Asialo, monogalactosylated biantennary complex N-glycan (NA2G1) GKC-014300 10 µg 1479 Porcine gamma globulin
Glyko™ Asialo-, agalacto-, biantennary, core-substituted with fucose (NGA2F) GKC-004301 10 µg  1463 Porcine thyroglobulin
Glyko™ Asialo-, agalacto-, triantennary (NGA3) GKC-005300 10 µg 1521 Bovine fetuin
Glyko™ Asialo-, agalacto-, tetraantennary (NGA4) GKC-006300 20 µg 1724 Human α1-acid glycoprotein
Glyko™ Asialo, monogalactosylated biantennary core substituted with fucose (NA2G1F) GKC-014301 10 µg 625.5 N/A
Glyko™ Asialo-, galactosylated biantennary (NA2) GKC-024300 10 µg  1642 Human fibrinogen
Glyko™ Asialo-, galactosylated biantennary, core-substituted with fucose and with bisecting GlcNAc (NA2FB) GKC-024311 10 µg  1991 Sheep IgG
Glyko® Oligomannose 9 (Man-9) GKM-002900 10 µg 1884 Porcine thyroglobulin
Glyko® Oligomannose 8 (Man-8) GKM-002800 10 µg 1722 Porcine thyroglobulin
Glyko® Oligomannose 7 (Man-7) GKM-002700 10 µg  1560 Porcine thyroglobulin
Glyko® Oligomannose 6 (Man-6) GKM-002600 10 µg  1398 Porcine thyroglobulin
Glyko® Oligomannose 5 (Man-5) GKM-002500 10 µg 1235 Porcine thyroglobulin
Glyko™ Asialo-, agalacto-, biantennary (NGA2) GKC-004300 10 µg 1317 Human fibrinogen
Glyko™ Asialo-, agalacto-, biantennary, core-substituted with fucose and with bisecting GlcNAc (NGA2FB) GKC-004311 10 µg 1667 Sheep IgG
Glyko™ Asialo-, galactosylated biantennary, core-substituted with fucose (NA2F) GKC-024301 10 µg  1788 Porcine thyroglobulin
Glyko™ Asialo-, galactosylated triantennary (NA3) GKC-035300 10 µg  2007 Bovine fetuin
Glyko™ Asialo-, galactosylated tetraantennary (NA4) GKC-046300 10 µg 2373 Human α1 acid glycoprotein
Glyko™ Mono-sialylated-, galactosylated biantennary (A1) GKC-124300 10 µg  1933 Human fibrinogen
Glyko™ Mono-sialylated-, galactosylated biantennary, core-substituted with fucose (A1F) GKC-124301 10 µg  2079 Porcine thyroglobulin
Glyko™ Di-sialylated-, galactosylated biantennary (A2) GKC-224300 10 µg  2224 Human fibrinogen
Glyko™ Di-sialylated-, galactosylated biantennary, core-substituted with fucose (A2F) GKC-224301 10 µg  2370 Porcine thyroglobulin
Glyko™ Tri-sialylated-, galactosylated triantennary (A3) GKC-335300 10 µg  2881 Bovine fetuin
Glyko® 6-α-fucosyl-N-acetylglucosamine (NF) GKR-001001 10 µg 367 Porcine thyroglobulin

 

产品类型 产品名称 产品编号 规格 分子量 主要来源
Hybrid-type Oligosaccharides Glyko® Hybrid-type N-Glycan with bisecting GlcNAc GKH-003510 10 µg N/A N/A
N-Linked Cores Glyko® Chitobiose (NN) GKR-002000 20 µg 424.4 N/A
Glyko® 6-α-fucosyl chitobiose (N2F) GKR-002001 100 µg 571 Synthesized
Glyko® 4′-β-mannosylchitobiose (MNN, MAN-1) GKR-002100 10 µg N/A N/A
Glyko® 4′-β-mannosylchitobiose core substituted with fucose (MNNF) GKR-002101 10 µg 732.7 Bovine fetuin
Glyko® Conserved trimannosyl core (M3N2, MAN-3) GKR-002300 10 µg 911 Bovine fetuin
Glyko® Conserved trimannosyl core, substituted with fucose (M3N2F) GKR-002301 10 µg 1057 Porcine thyroglobulin
Neutral O-Linked Oligosaccharides Glyko® Lacto-N-difucohexaose I (LNDFH I) GKAD-02010 250 µg pack 1000 Human milk 
Fucosyl-p-lacto-N-hexaose GKAD-00125 100 µg N/A N/A
Glyko® Difucosyllacto-N-hexaose (DFLNH(a)) GKAD-00131 100 µg 1365 Human milk
Glyko® Monofucosyllacto-N-hexaose III (MFLNH-III) GKAD-00133 100 µg 1219 Human milk
Glyko® Trifucosyllacto-N-hexaose (TFLNH) GKAD-00135 100 µg 1511 Human milk
Glyko® Lacto-N-fucopentaose III (LNFP III) GKAD-00508 500 µg 854 Human milk
Glyko® Lacto-N-fucopentaose V (LNFP V) GKAD-00509 500 µg 854 Human milk
Glyko® Lacto-N-difucohexaose II (LNDFH II) GKAD-00511 500 µg 1000 Human milk
Glyko® Lacto-N-hexaose (LNH) GKAD-00523 500 µg 1073 Human milk
Glyko® Lacto-N-fucopentaose II (LNFP II) GKAD-01007 1 mg 854 Human milk
Glyko® Lacto-N-neohexaose (LNnH) GKAD-01024 250 µg 1073 Human milk
Glyko® 2′-Fucosyllactose (2′-FL) GKAD-02001 2 mg 487 Human milk
Glyko® 3-Fucosyllactose (3-FL) GKAD-02002 1 mg 487 Human milk
Glyko® Lactodifucotetraose (LDFT) GKAD-02003 2 mg 635 Human milk
Glyko® Lacto-N-tetraose (LNT) GKAD-02004 0.5 mg 708 Human milk
Glyko® Lacto-N-neotetraose (LNnT) GKAD-02005 500 µg 708 Human milk
Glyko® Lacto-N-fucopentaose I (LNFP I) GKAD-02006 500 µg 854 Human milk

 

产品类型 产品名称 产品编号 规格 分子量 主要来源
Sialylated O-Linked Oligosaccharides Glyko® LS-Tetrasaccharide b (LST b) GKAD-01018 0.5 mg 999 Human milk
Glyko® 3′-Sialyl-3-fucosyllactose (3′-S,3-FL) GKAD-00116 100 µg 780 Human milk
Glyko® 3′-Sialyl-N-acetyllactosamine (3′-SLN) GKAD-01014 500 µg 675 Urine
Glyko® 6′-Sialyl-N-acetyllactosamine (6′-SLN) GKAD-01015 500 µg 675 Urine
Glyko® LS-Tetrasaccharide a (LST a) GKAD-01017 1 mg 999 Human milk
Glyko® 3′-Sialyllactose (3′-SL) GKAD-02012 2 mg 634 Human milk and urine
Glyko® 6′-Sialyllactose (6′-SL) GKAD-02013 500 µg 634 Human milk
Glyko® LS-Tetrasaccharide c (LST c) GKAD-02019 250 µg 999 Human milk
Glyko® Disialyllacto-N-tetraose (DSLNT) GKAD-02020 250 µg 1290 Human milk
Blood Group Oligosaccharides Glyko® Blood group A trisaccharide GKAD-00141 5 mg 530 Chemical Synthesis
Glyko® Blood group B trisaccharide GKAD-00143 5 mg 488 Chemical Synthesis
Glyko® Blood group H disaccharide GKAD-00144 1 mg 326 Chemical Synthesis
Glyko® Blood group H disaccharide GKAD-00145 5 mg N/A N/A
Lewis Antigens 3′-Sialyl Lewis x GKAD-00126 100 µg 821 Synthesized
Lewis x GKAD-00127 100 µg 530 Synthesized
3′-Sialyl Lewis a GKAD-00128 100 µg N/A N/A
Lewis a GKAD-00129 100 µg 530 Synthesized
Glyko® 3′-Sulfated Lewis a GKAD-00147 1 mg 610 Synthesized
Glyko® Lewis b (Leb) GKAD-00149 1 mg 676 Synthesized
Sialylated Oligosaccharides found in Human Milk GlycoSet II GKGS-312 1 ea  1vial(100μg):3-SL;6-SL; 3-SLN; 6-SLN; 3-S,3-FL; LSTa; LSTb;LSTc; DSLNT; NeuNAc
N-Linked Oligosaccharide Libraries Glyko® Human α-1-acid glycoprotein N-Linked Glycan Library GKLB-001 1 ea  N/A N/A
Glyko® Bovine Fetuin N-linked Glycan Library GKLB-002 1 ea  N/A N/A
Glyko® Human IgG N-Linked Glycan Library GKLB-005 1 ea  N/A N/A
Glucose Homopolymer Standard Glucose Homopolymer Standard GKI-4503 10 ug N/A N/A
Reference Panels

Glyko® Sialic Acid Reference Panel

GKRP-2503 1 ea (6.25nmole) 5 vials (1.25 nmoles/vial): Neu5Gc; Neu5Ac; Neu5,7Ac2; Neu5Gc, 9Ac; Neu5,9Ac2; Neu,(7/8)9Ac3
Glyko® Monosaccharide Standard Set GKRP-3500 1 ea Galactose, Mannose, Glucose, Fucose, N-Acetylglucosamine, N-Acetylgalactosamine

 

糖标准品(含标记)

 

产品类型 产品名称 产品编号 规格
InstantAB™ Labeled Oligosaccharides Glyko® InstantAB™ Human IgG N-Linked Glycan Library GKIB-005 1 ea (70pmol)
Glyko® InstantAB™-(RNAse B N-Linked Glycan Library) GKIB-009 1 ea (70pmol)
Glyko® InstantAB™-(Bovine Fetuin N-Linked Glycan Library) GKIB-002 1 ea (70pmol)
Glyko® InstantAB™-(Human α1-Acid Glycoprotein N-Linked Glycan Library) GKIB-001 1 ea (70 pmol)
Glyko® InstantAB™-(Glucose Homopolymer) GKIB-503 1 ea (200 pmol)
InstantAA™ Labeled Oligosaccharides Glyko® InstantAA™ Glucose Homopolymer Standard GKIA-503 1 ea (200 pmol)
2-AB (2-Aminobenzamide) Labeled Oligosaccharides Glyko® 2-AB- asialo-, mono-galactosylated biantennary (2-AB NA2G1) GKSB-317 1 ea (100 pmol)
Glyko™ 2-AB markers, 2′-FL and 3′-SL GKI-4723 1 ea (1 ea)
Glyko® 2-AB-(Human α-1-acid glycoprotein N-Linked Glycan Library) GKSB-001 ea (200 pmol)
Glyko® 2-AB-(Bovine Fetuin N-linked Glycan Library) 200pmol GKSB-002 1 ea (200 pmol)
Glyko® 2-AB-(Human IgG N-Linked Glycan Library) GKSB-005 1 ea (200 pmol)
Glyko® 2-AB Chitobiose GKSB-100 1 ea (100 pmol)
Glyko® 2-AB conserved trimannosyl core (2-AB M3N2) GKSB-101 1 ea (100 pmol)
Glyko® 2-AB conserved trimannosyl core substituted with fucose(2-AB M3N2F) GKSB-102 1 ea (100 pmol)
Glyko® 2-AB oligomannose 5 (2-AB Man-5) GKSB-103 1 ea (100 pmol)
Glyko® 2-AB oligomannose 6 (2-AB Man-6) GKSB-104 1 ea (100 pmol)
Glyko® 2-AB oligomannose 7 (2-AB Man-7) GKSB-105 1 ea (100 pmol)
Glyko® 2-AB oligomannose 8 (2-AB Man-8) GKSB-106 1 ea (100 pmol)
Glyko® 2-AB oligomannose 9 (2-AB Man-9) GKSB-107 1 ea (100 pmol)
Glyko® 2-AB hybrid-type GKSB-111 1 ea (100 pmol)
Glyko® 2-AB GalGalNAc GKSB-201 1 ea (100 pmol)
Glyko® 2-AB 3′-sialyl-N-acetyllactosamine (2-AB 3′-SLN) GKSB-203 1 ea (100 pmol)
Glyko® 6′-sialyl-N-acetyllactosamine (2-AB 6′-SLN) GKSB-204 1 ea (100 pmol)
Glyko® 2-AB asialo-, agalacto-, biantennary (2-AB NGA2) GKSB-301 1 ea (100 pmol)
Glyko® 2-AB asialo-, agalacto-, biantennary, core-substituted with fucose (2-AB NGA2F) GKSB-302 1 ea (100 pmol)
Glyko® 2-AB asialo- agalacto- biantennary core-sub with fucose and with bisecting GlcNAc (2-AB NGA2FB) GKSB-303 1 ea (100 pmol)
产品类型 产品名称 产品编号 规格
2-AB (2-Aminobenzamide) Labeled Oligosaccharides Glyko® 2-AB asialo-, galactosylated biantennary (2-AB NA2) GKSB-304 1 ea (100 pmol)
Glyko® 2-AB asialo-, galactosylated biantennary, core-substituted with fucose (2-AB NA2F) GKSB-305 1 ea (100 pmol)
Glyko® 2-AB asialo-, galactosylated biantennary, core-sub w/ fucose, w/ bisecting GlcNAc (2-AB NA2FB) GKSB-306 1 ea (100 pmol)
Glyko® 2-AB asialo-, agalacto-, triantennary (2-AB NGA3) GKSB-307 1 ea (100 pmol)
Glyko® 2-AB asialo-, galactosylated triantennary (2-AB NA3) GKSB-308 1 ea (100 pmol)
Glyko® 2-AB asialo-, agalacto-, tetraantennary (2-AB NGA4) GKSB-309 1 ea (100 pmol)
Glyko® 2-AB asialo-, galactosylated tetraantennary (2-AB NA4) GKSB-310 1 ea (100 pmol)
Glyko® 2-AB mono-sialylated-, galactosylated biantennary (2-AB A1) GKSB-311 1 ea (100 pmol)
Glyko® 2-AB di-sialylated-, galactosylated biantennary (2-AB A2) GKSB-312 1 ea (100 pmol)
Glyko® 2-AB di-sialylated-, galactosylated biantennary, core-substituted with fucose (2-AB A2F) GKSB-313 1 ea (100 pmol)
Glyko® 2-AB tri-sialylated-, galactosylated triantennary (2-AB A3) GKSB-314 1 ea (100 pmol)
Glyko® 2-AB mono-sialylated-, galactosylated biantennary, core-substituted with fucose (2-AB A1F) GKSB-315 1 ea (100 pmol)
Glyko® 2-AB Asialo, monongalactosylated biantennary core substituted with fucose (2-AB NA2G1F) GKSB-316 1 ea (100 pmol)
Glyko® 2-AB Glucose Homopolymer Standard GKSB-503 1 ea (200 pmol)
2-AA (2-Aminobenzoic acid) Labeled Oligosaccharides Glyko® 2-AA-(Human α1-Acid Glycoprotein N-Linked Glycan Library) GKSA-001 1 ea (200 pmol)
Glyko® 2-AA-(Bovine Fetuin N-linked Glycan Library) GKSA-002 1 ea (200 pmol)
Glyko® 2-AA-(Human IgG N-Linked Glycan Library) GKSA-005 1 ea (200 pmol)
Glyko® 2-AA Oligomannose 5 (2-AA Man-5) GKSA-103 1 ea (100 pmol)
Glyko® 2-AA Oligomannose 6 (2-AA Man-6) GKSA-104 1 ea (100 pmol)
Glyko® 2-AA Oligomannose 7 (2-AA Man-7) GKSA-105 1 ea (100 pmol)
Glyko® 2-AA Oligomannose 8 (2-AA Man-8) GKSA-106 1 ea (100 pmol)
Glyko® 2-AA Oligomannose 9 (2-AA Man-9) GKSA-107 1 ea (100 pmol)
Glyko® 2-AA asialo, agalacto biantennary (2-AA NGA2) GKSA-301 1 ea (100 pmol)
Glyko®2-AA asialo, agalacto biantennary core substituted with fucose) (2-AA NGA2F) GKSA-302 1 ea (100 pmol)
Glyko® 2-AA asialo, galactosylated biantennary (2-AA NA2) GKSA-304 1 ea (100 pmol)
2-AA NA2F GKSA-305 1 ea (100 pmol)
2-AA NGA3 GKSA-307 1 ea (100 pmol)
产品类型 产品名称 产品编号 规格
2-AA (2-Aminobenzoic acid) Labeled Oligosaccharides 2-AA NA3 GKSA-308 1 ea (100 pmol)
2-AA A1 GKSA-311 1 ea (100 pmol)
2-AA A2 GKSA-312 1 ea (100 pmol)
2-AA A1F GKSA-315 1 ea (100 pmol)
2-AA NA2G1F GKSA-316 1 ea (100 pmol)
Glyko® 2-AA Glucose Homopolymer Standard GKSA-503 1 ea (200 pmol)

凝集素&糖生物学试剂

凝集素&糖生物学试剂

  凝集素概述:

凝集素(Lectin)是指一种从各种植物,无脊椎动物和高等动物中提纯的糖蛋白或结合糖的蛋白。其具有对某一种特异性糖基专一性结合的能力(类似于抗原-抗体反应)。因此,凝集素在生物识别(例如,细胞与蛋白识别)中发挥了重要的作用,例如:一些病毒利用凝集素将其附着在宿主细胞上、凝集素可识别某些致病真菌等。

 

随着多种生物技术手段不断发展,利用凝集素进行研究的新方法也逐渐崭露头角。除了传统的组织/细胞免疫实验、Western bloting、酶联免疫检测(ELISA)以及细胞培养等实验中的应用,凝集素也在更多新的领域,Flow Cytometry(细胞分型)、亲和层析、表面等离子体共振(SPR)等领域发挥其作用。目前,Vector Labs的凝集素产品已被美国国家糖组学功能研究中心(NCFG)指定为首选产品。

  凝集素应用:

组织学应用(IHC) 组织学应用(IF) 亲和层析

凝集素&糖生物学试剂

凝集素&糖生物学试剂

凝集素&糖生物学试剂

炎性结肠炎模型中组织糖基化检测与治疗 弓形虫凝集素染色(VL-FITC labelled) 凝集素对不同HIV糖肽的免疫反应(GNL-Agarose labelled)

Flow Cytometry(流式实验) 酶联免疫吸附(ELISA)

凝集素&糖生物学试剂

凝集素&糖生物学试剂

血小板细胞表面蛋白糖基化分析 利用凝集素对IVIG Fc片段糖基化进行筛选(ELISA)

蛋白免疫印迹(Western Bloting) 酶分析——糖苷转移酶/糖苷酶

凝集素&糖生物学试剂

凝集素&糖生物学试剂

细胞成熟过程中蛋白糖基化差异性检测(PNA-biotin) 活化的糖基转移酶过量表达导致其受体失活

血管灌注 表面等离子体共振(SPR)

凝集素&糖生物学试剂

凝集素&糖生物学试剂

大鼠心肌梗死细胞血管可视化观察(LEL- Texas Red) SNA凝集素与合成底物结合的表征

神经示踪 细胞培养——增殖/活性/毒性研究

凝集素&糖生物学试剂

凝集素&糖生物学试剂

蛙视网膜神经元示踪(PHA-L) 凝集素毒性可引起细胞表面蛋白糖基化的变化

  产品类别:

凝集素&糖生物学试剂

凝集素筛选试剂盒

凝集素&糖生物学试剂

凝集素
凝集素筛选试剂盒包含多种凝集素(Purified或其他偶联形式),可识别不同的糖链结构。可满足需要进行高通量实验的检测需求。
产品货号:Cat#BK-1000, BK-2100,BK-3000等
产品特性:均来自天然提取的植物凝集素
产品种类:40+,是凝集素种类最齐全的供货商
产品形式:多种标记(生物素、FITC、PE、CY3、CY5、Agarose以及纯化形式等)

凝集素&糖生物学试剂

抗凝集素抗体

其他辅助试剂
搭配purified或biotin标记的凝集素一起使用。在某些特定应用中,使用抗凝集素抗体比直接使用其他偶联物标记的抗体有更高的灵敏度。 主要有两类产品:
1.Inhibitor sugars:作为阴性对照
2.Elutiong solutions:洗脱试剂,用于琼脂糖连接凝集素的洗脱,或者其他实验应用

  热销凝集素举例:

花生凝集素:PeanutAgglutinin(PNA)
Unconjugated L-1070 5mg,25mg
Agarose AL-1073 2mL,5mL
Biotin B-1075 5mg
CY3 CL-1073 1mg
CY5 CL-1075 1mg
Fluorescein FL-1071 5mg,10mg
Rhodamine RL-1072 5mg

菜豆凝集素:PhaseolusVulgarisAgglutinin(PHA) 
PhaseolusvulgarisErythroagglutinin(PHA-E)
Unconjugated L-1120 5mg
Biotin B-1125 2mg
Fluorescein FL-1121 2mg
PhaseolusvulgarisLeucoagglutinin(PHA-L)
Unconjugated L-1110 5mg
Biotin B-1115 2mg
Fluorescein FL-1111 2mg
Rhodamine RL-1112 2mg

伴刀豆凝集素:ConcanavalinA(ConA)
Unconjugated L-1000 500mg
Agarose AL-1003 10mL,100mL
Biotin B-1005 5mg
Fluorescein FL-1001 25mg
Rhodamine RL-1002 25mg

番茄凝集素:LycopersiconEsculentum(Tomato)Lectin(LEL,TL)
Unconjugated L-1170 2mg
Biotin B-1175 1mg
DyLight488 DL-1174 1mg
DyLight594 DL-1177 1mg
DyLight649 DL-1178 1mg
Fluorescein FL-1171 1mg
TexasRed TL-1176 1mg

接骨木凝集素:SambucusNigraLectin(SNA,EBL)
Unconjugated L-1300 5mg
Agarose AL-1303 2mL
Biotin B-1305 2mg
CY3 CL-1303 1mg
CY5 CL-1305 1mg
Fluorescein FL-1301 2mg

荆豆凝集素:UlexEuropaeusAgglutininI(UEAI)
Unconjugated L-1060 2mg,5mg
Agarose AL-1063 2mL
Biotin B-1065 2mg
DyLight594 DL-1067 1mg
DyLight649 DL-1068 1mg
Fluorescein FL-1061 2mg,5mg
Rhodamine RL-1062 2mg

麦胚凝集素:WheatGermAgglutinin(WGA)
Unconjugated L-1020 10mg,25mg
Agarose AL-1023 2mL,5mL,10mL
Biotin B-1025 5mg
Fluorescein FL-1021 5mg,10mg
Peroxidase PL-1026 2mg
Rhodamine RL-1022 5mg,10mg

详情请咨询 Vectorlabs 中国代理- 上海金畔生物科技

凝集素全能小帮手 — 5种方式助力糖生物学研究

凝集素全能小帮手 — 5种方式助力糖生物学研究

糖蛋白(聚糖)以及生物结合物通过介导细胞与细胞间的相互作用,对生物机体发挥至关重要的作用。糖链结构的变化在许多疾病,包括肿瘤、糖尿病以及自身免疫性疾病等中发挥核心作用。但是,目前只有很小一部分的生物标志物是与细胞表面的糖蛋白相关。主要原因是单糖形成聚糖的方式多样性和复杂性,导致对复杂糖蛋白(例如,凝集素)结构的研究进展很慢。

凝集素(Lectin)一词源于拉丁语”legere”,意思是“选择”,指它们具有识别并结合不同碳水化合物结构的能力。但是,为什么这种特性使其在糖生物学上有如此应用前景呢?

随着生物标志物研究的不断发展,利用凝集素进行研究的新方法也逐渐崭露头角。本文总结了凝集素作为糖生物学研究工具的5种主要应用。

1.凝集素可灵活应用于组织/细胞免疫组化实验

To elicit biologically-relevant information about aberrant glycosylation, you need to visualize cell glycan distribution and its impact on morphology. Fortunately, lectins are compatible tools for molecular imaging protocols, such as immunohistochemistry (IHC) and immunofluorescence (IF), as the tissue staining protocols for antibodies are mostly valid for lectins. Both workflows are simple to implement, producing rapid results. 

In your IHC workflow, you can conjugate a lectin to enzymes (e.g., horseradish peroxidase) or haptens (e.g., biotin). The resulting lectin conjugate and appropriate substrate can be viewed under light microscopy or electron microscopy.  

Your other option for your IF workflow, label lectins with a fluorescent dye (e.g., fluorescein isothiocyanate (FITC)), which allows you to view glycan distribution with a fluorescent microscope. The use of lectins in IF has the added advantage of multiplexing, which allows you to use multiple fluorescent dyes to visualize multiple types of glycan chains in the same sample.

2.可实现使用最小的样本量进行高效的聚糖分析

Scanning a sample for multiple glycan sequences can be time consuming without high-throughput analysis methods. Lectins can provide time-saving and cost-effective analysis with the help of very small solid surfaces, such as microchips.  

Because you want a comprehensive glycan profiling of your sample, you need to detect as many different glycan structures as possible. Compared to antiglycan antibodies which have stringent specificity towards glycans, making them more time-consuming to use, lectins are more versatile in their binding patterns, meaning that one lectin can bind multiple glycans (1). This makes lectins more effective than antibodies as microarray tools.

3.无需糖链释放——直接进行聚糖检测

One of the advantages of lectins is their ability to detect glycans without the need to release them from their bioconjugates. Lectin blotting, based on western blotting, is a method that provides insight into glycan structures while they are still attached to their glycoproteins. Intact glycoproteins or glycolipids can be probed with lectins and there is no requirement for cleaving the whole complex. This also makes lectin blotting a promising tool for comparing glycosylation networks between control and test samples. For instance, researchers could demonstrate altered sialylation and fucosylation of N-glycans in colorectal cancer by comparing biofluid samples from cancer patients to those from healthy subjects (2).  

Lectin microarray is another method for rapid glycan detection that does not require glycan release. This allows you to detect and differentiate all possible disease-associated glycan isomers, aberrant sialic acid linkages, and terminal glycan structures within glycoproteins and glycolipids. For example, lectin microarray was used extensively to help researchers discover predictive biomarkers in several cancer types, such as colorectal (3) and gastric cancer (4).

4.可用于活细胞分析(Flow-Cytometry流式实验应用)

Understanding the correlation between specific glycan structures and cell characteristics is necessary for elucidating cell size, proliferation, and differentiation. To obtain biologically-relevant data, you should be able to work with live cells. The great news is that you can integrate fluorescently labelled lectins into flow cytometry protocols to perform live-cell imaging.  

The use of lectins in flow cytometry is advantageous because it offers an opportunity for quantitative analysis of glycan profiles in different cell subtypes. Lectins can help characterize cellular subpopulations even when a specific cell type is scarce in the overall cell population.  

The combination of lectins and flow cytometry led to many breakthroughs in stem cell research. Using lectins in flow cytometry, researchers were able to successfully characterize human embryonic stem cells (hESCs) and their differentiated progeny based on lectin-binding profiles. This also allowed them to isolate neural progenitor cells to analyze their role in brain development in detail (5).

5.可有效分离糖蛋白

Imagine wanting to analyze a specific aberrant glycoprotein in a sample, but the concentration of that particular glycoprotein is low. The solution is to run affinity chromatography to obtain eluates enriched with the glycoprotein of your interest.  

In lectin affinity chromatography, you can utilize the carbohydrate specificity of lectins by immobilizing them on your chromatography surface or matrix. Upon washing, the immobilized lectin binds the corresponding glycoprotein while the rest of the sample is washed away. The resulting elution is now suitable for mass spectroscopy and proteomics, generating further insight into the glycosylation mechanism of your protein. For example, lectin affinity chromatography played a significant role in the enrichment of core fucosylated peptides that are potential biomarkers in pancreatic cancer (6). 

结论

凝集素因其独特的理化特性使其成为研究细胞膜表面糖蛋白结构、功能及其对复杂疾病影响的理想工具。搭配糖样本制备平台和分析技术,可快速实现糖样本的分析,获得高度一致和可靠的数据结果。

目前,利用凝集素识别和分析糖蛋白样本的方法有很多,因此需要根据具体的实验需求选择最合适的产品。Vector Laboratories公司旨在通过对凝集素工作流程的详细阐述,为广大用于提供尽可能多的信息和支持。想要了解更多的凝集素产品的应用,可访问https://vectorlabs.com/browse/lectins 或咨询联系Vector Laboratories中国代理商上海金畔生物科技有限公司 http://www.jinpanbio.com/

Glyko® 糖生物学研究工具-糖苷酶

Glyko® 糖生物学研究工具-糖苷酶

糖苷酶

 

内切糖苷酶  

内切糖苷酶可催化水解寡糖及多糖的内部糖苷键,其中内切糖苷酶F可断裂糖蛋白的天冬酰胺和甘露聚糖的糖苷键,内切糖苷酶H专一水解两个N-乙酰氨基葡糖之间的 β(1,4)糖苷键,其底物主要是高甘露糖型和杂合型糖链。

产品类型 产品名称 产品编号 规格
PNGaseF N-Glycanase® GKE-5006A 1 ea (100mU*in 40 µl)
N-Glycanase® GKE-5006B 1 ea (200mU in 80 µl)
N-Glycanase® GKE-5006D 1 ea (1U in 400µl)
N-Glycanase®-Ultra GKE-5020B 1 ea (400mU in 40µl)
N-Glycanase®-Ultra GKE-5020D 1 ea (1U in 100µl)
Glyko® N-Glycanase-PLUS GKE-5010B 1 ea (400mU in 40µl)
Glyko® N-Glycanase-PLUS GKE-5010D 1 ea (1U in 100µl)
Glyko® PNGase F (Chryseobacterium [Flavobacterium] meningosepticum) GKE-5003 1 ea (100 mU (50 µl))
Endo-H Glyko® Endoglycosidase H (recombinant gene from Streptomyces plicatus, expressed in E. coli) GKE-5002 1 ea (30 mU (200 µl))
Endo F2 Glyko® Endo F2 (recomb Chryseobacterium [Flavobacterium] meningosepticum expressed in E. coli) GKE-5008 1 ea (6 mU (60 µl))
PNGaseA Glyko® PNGase A (almond) GKE-5011A 1 ea (2mU)
Glyko® PNGase A (almond) GKE-5011B 1 ea (10mU)
O-Glycanase Glyko® O-Glycanase (recombinant gene from Streptococcus pneumoniae, expressed in E.coli) GK80090 1 ea (50 mU (40 µl))

 

* Prozyme Unit=1 IUB Unit

 

切糖苷酶

 

产品类型 产品名称 产品编号 规格
唾液酸酶 Glyko® Sialidase A™ Sampler Kit GK80043 1 ea (1 ea)
Glyko® Sialidase A™/NANase III (recombinant gene from Arthrobacter ureafaciens, expressed in E. coli) GK80040 1 ea (1 U (200 µl))
Glyko® Sialidase A™-51 (recombinant gene from Arthrobacter ureafaciens, expressed in E. coli) GK80045 1 ea (1 U (200 µl))
Glyko® Sialidase A™-66 (recombinant gene from Arthrobacter ureafaciens, expressed in E. coli) GK80046 1 ea (1 U (200 µl))
Glyko® Sialidase I (recombinant gene from Clostridium perfringens, expressed in E. coli) GKX-5021 1 ea (1 U (200 µl))
Glyko® Sialidase C™/NANase II (recombinant from Clostridium perfringens, expressed in E. coli) GK80030 1 ea (1 U (100 µl))
Glyko® Sialidase V™ (Vibrio cholerae) GKX-5020 1 ea (1 U (200 µl))
Glyko® Sialidase T™ (recombinant gene from Salmonella typhimurium, expressed in E. coli) GKX-5018 1 ea (10 U)
Glyko® Sialidase S™/NANase I (recombinant from Streptococcus pneumoniae, expressed in E. coli) GK80020 1 ea (1 U)
β-半乳糖苷酶 Glyko® β(1,3,6)-Galactosidase/GALase II (recomb from Xanthomonas manihotis, expressed in E. coli) GK80120 1 ea (200 mU (40 µl))
Glyko® β(1-4,6)-Galactosidase (Jack bean) GKX-5012 1 ea (5 U lyophilized)
Glyko® β(1-3,4)-Galactosidase (bovine testis) GKX-5013 1 ea (0.5 U)
Glyko® β(1-4)-Galactosidase (Streptococcus pneumoniae) GKX-5014 1 ea (200 mU (100 µl))
氨基己糖苷酶  Glyko® β-N-Acetylhexosaminidase (jack bean)/HEXase III GKX-5003 1 ea (5 U )
Glyko® β-N-Acetylhexosaminidase/HEXase I (recombinant gene from Streptococcus pneumoniae, expressed in E. coli) GK80050 1 ea (1.6 U)
氨基半乳糖苷酶 Glyko® α-N-Acetylgalactosaminidase (chicken liver) GKX-5001 1 ea (100 mU)
α-甘露糖苷酶 Glyko® α(1-3,4,6)-Galactosidase (green coffee bean) GKX-5007 1 ea (5 U lyophilized)
Glyko® α(1-2,3,6)-Mannosidase (jack bean) GKX-5010 1 ea (10 U)
Glyko® α(1-2)-Mannosidase (Aspergillus saitoi) GKX-5009 1 ea (2 mU lyophilized)
Glyko® α-Mannosidase/MANase VI (recombinant gene from Xanthomonas manihotis, expressed in E. coli) GK80070 1 ea (8 mU)
β-甘露糖苷酶 Glyko® β-Mannosidase (Helix pomatia) GKX-5015 1 ea (1 U lyophilized)
α-岩藻糖苷酶 Glyko® α(1-3,4)-Fucosidase (almond meal) GKX-5019 1 ea (200 µU lyophilized)
Glyko® α(1-2)Fucosidase/FUCase II (Xanthomonas manihotis) GK80170 1 ea (4 mU)
Glyko® α(1-2,3,4,6)Fucosidase (bovine kidney) GKX-5006 1 ea (500 mU lyophilized)