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产品信息:
经Nuclear Fast Red (cat#H-3403)复染的细胞核呈粉红色。其尤其适用于多抗原标记的多重染色,可与其他靶标蛋白形成鲜明的颜色对比。此外,该产品为即用型试剂,使用方法简单,操作快速。
产品特点:
1. 简单、快速,一步即可完成染色;
2. 多抗原标记的最佳选择,可避免苏木精导致的模糊染色;
3. 与多种底物相兼容,并形成良好的颜色对比;
4. 适用于非水溶性封片剂
应用举例:
上图为肿瘤特异性细胞质的染色结果(SG,蓝色/灰色),使用Nuclear Fast Red(H-3403)复染(粉红色)
产品订购信息:
产品 | 货号 | 规格 | 性质 |
Nuclear Fast Red | H-3403 | 500ml | 非水溶性 |
附 Vector的核复染试剂与底物的兼容性:
Substrate | Catalog Number |
Vector Hematoxylin and Hematoxylin QS H-3401 and H-3404 |
Vector Methyl Green H-3402 |
Vector Nuclear Fast Red H-3403 |
ImmPACT DAB (brown) | SK-4105 | Excellent Contrast | Excellent Contrast | Fair Contrast |
ImmPACT DAB EqV | SK-4103 | Excellent Contrast | Excellent Contrast | Fair Contrast |
DAB (brown) | SK-4100 | Excellent Contrast | Excellent Contrast | Fair Contrast |
DAB-Ni (gray-black) | SK-4100 | Excellent Contrast | Fair Contrast * | Good Contrast |
ImmPACT AEC (red) | SK-4205 | Excellent Contrast | Counterstain Incompatibility ** | Color Incompatibility |
ImmPACT AMEC Red (red) | SK-4285 | Excellent Contrast | Counterstain Incompatibility ** | Color Incompatibility |
AEC (red) | SK-4200 | Excellent Contrast | Counterstain Incompatibility ** | Color Incompatibility |
TMB (blue) | SK-4400 | Color Incompatibility | Counterstain Incompatibility | Excellent Contrast |
ImmPACT VIP (purple) | SK-4605 | Fair Contrast | Excellent Contrast | Poor Contrast |
Vector VIP (purple) | SK-4600 | Fair Contrast | Excellent Contrast | Poor Contrast |
ImmPACT SG (blue-gray) | SK-4705 | Poor Contrast | Good Contrast | Excellent Contrast |
SG (blue-gray) | SK-4700 | Poor Contrast | Good Contrast | Excellent Contrast |
ImmPACT NovaRED (red) | SK-4805 | Excellent Contrast | Excellent Contrast *** | Color Incompatibility |
Vector NovaRED (red) | SK-4800 | Excellent Contrast | Excellent Contrast *** | Color Incompatibility |
ImmPACT Vector Red (magenta) | SK-5105 | Excellent Contrast | Excellent Contrast | Color Incompatibility |
Vector Red (magenta) | SK-5100 | Excellent Contrast | Excellent Contrast | Color Incompatibility |
Vector Black (black) | SK-5200 | Excellent Contrast | Excellent Contrast * | Excellent Contrast |
Vector Blue (blue) | SK-5300 | Color Incompatibility | Good Contrast | Excellent Contrast |
BCIP/NBT (indigo) | SK-5400 | Color Incompatibility | Excellent Contrast * | Excellent Contrast |
复染——作为免疫组化实验的最后一个步骤,其重要性不言而喻。
通过复染,可对特定的细胞结构进行染色,有助于实现对靶标蛋白的定位以及可视化观察,更有利于阐述组织切片形态学特征和细胞内部结构。Vectorlabs公司可提供多种核复染试剂:Hematoxylin、Hematoxylin QS、Methyl Green、Nuclear Fast Red等。产品包装方便、均为即用型试剂,可用于单个载玻片或整个染色皿的染色。
Vector Laboratories是一家致力于免疫组织化学、免疫荧光、糖生物学和生物结合的标记和检测试剂研发和生产的先驱,现已成为该领域的领导者。其产品线包括:ABC免疫组化试剂盒、ImmPRESS多聚合物检测试剂盒、防荧光淬灭封片剂、二抗、酶底物、亲和素/链霉亲和素-酶结合物、封闭试剂等。此外,近期还推出了多抗原染色、新品VectaMount封片剂、M.O.M/H.O.H等特色产品。Vector的设施通过了ISO 9001:2015认证,其研究成果已被350,000多种科学出版物引用。
热销产品举例:
产品 | 货号 | 规格 | 说明 |
VECTASHIELD® Antifade Mounting Medium with DAPI, 10 ml | H-1200 | 10ml | 最火爆的封片剂 |
ImmEdge® Hydrophobic Barrier (PAP) Pen, 2-Pen set | H-4000 | 2-Pen Set* | 最好用的组化笔 |
VECTASTAIN® Elite ABC-HRP Kit, Peroxidase (Standard), 1 Kit | PK-6100 | 1kit | ABC试剂盒金标准 |
DAB Substrate Kit, Peroxidase (HRP), with Nickel, (3,3′-diaminobenzidine), 1 Kit | SK-4100 | 1kit | 性价比最高的底物 |
Vector® TrueVIEW® Autofluorescence Quenching Kit, 1 kit | SP-8400 | 1kit | 防应光淬灭神器 |
Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated, 1.5 mg | BA-1000-1.5 | 1.5mg | 最畅销的二抗 |
详情请咨询 Vectorlabs 中国代理- 上海金畔生物科技