ROS-ID®一氧化氮检测试剂盒——ENZO热销产品
Enzo Life Sciences的ROS-ID® NO Detection kit以红色荧光的NO检测试剂为主要成分，可通过荧光显微镜直接实时监测活细胞中NO的产生。这种非荧光的、可渗透细胞的NO检测染料在O2存在的情况下能够与NO发生反应，具有高特异性、灵敏性和准确性，产生不溶于水的红色荧光产物。这种染料对过氧亚硝酸盐没有反应性，因此可以区分过氧亚硝酸盐和一氧化氮。
ROS-ID® NO Detection kit中的荧光探针对活性氯或溴不敏感，因此不用于检测这些分析物。染色后，该染料产生的荧光产物可通过荧光显微镜进行观察。当需要同时检测额外的荧光信号（绿色或橙色）时，建议使用650/670 nm的组合。ROS-ID® NO Detection kit包含足够的试剂，可对活细胞（贴壁或悬浮）进行至少200次检测。
产品特色
通过荧光显微镜直接实时监测活体细胞中NO的产生
高特异性、灵敏性和准确性，产生不溶于水的红色荧光产物
可区分过氧亚硝酸盐和一氧化氮
试剂盒内包含一氧化氮诱导剂和清除剂
产品信息
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产品货号
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ENZ-51013-200
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产品名称
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ROS-ID® NO Detection kit
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规格
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1*1Kit
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操作
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Protect from light. Avoid freeze/thaw cycles.
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短期保存
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-20°C
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长期保存
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-80°C
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试剂盒组分
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NO Detection Reagent (Red), 60 µl
NO Inducer (L-Arginine), 100 µl
NO Scavenger (c-PTIO), 400 nmoles
10X Wash Buffer, 15 ml
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应用
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Fluorescence microscopy, Fluorescent detection
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部分产品引用文献
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2. Noncanonical Role of Telomerase in Regulation of Microvascular Redox Environment With Implications for Coronary Artery Disease: K.A. Aissa, et al.; Function 3, zqac043 (2022)
3. S1P (Sphingosine-1-Phosphate)-Induced Vasodilation in Human Resistance Arterioles During Health and Disease: B. Katunaric, et al.; Hypertension 79, 2250 (2022)
4. High-content analysis monitoring intracellular trafficking and replication of Mycobacterium tuberculosis inside host cells: N. Debousere, et al.; Methods Mol. Biol. 2314, 649 (2021)
5. Hyperhomocysteinemia and Low Folate and Vitamin B12 Are Associated with Vascular Dysfunction and Impaired Nitric Oxide Sensitivity in Morbidly Obese Patients: M. Haloul, et al.; Nutrients 12, 2014 (2020)
6. Stimulation of TRPA1 attenuates ischemia-induced cardiomyocyte cell death through an eNOS-mediated mechanism: S.R. Andrei, et al.; Channels (Austin) 13, 192 (2019)
7. Anti-inflammatory effects of olive-derived hydroxytyrosol on lipopolysaccharide-induced inflammation in RAW264.7 cells: Y. Yonezawa, et al.; J. Vet. Med. Sci. 80, 1801 (2018)
8. Oligodendrocyte RasG12V expressed in its endogenous locus disrupts myelin structure through increased MAPK, nitric oxide, and notch signaling: H.E. Titus, et al.; Glia 65, 1990 (2017)
9. Improved arterial flow-mediated dilation after exertion involves hydrogen peroxide in overweight and obese adults following aerobic exercise training: A.T. Robinson, et al.; J. Hypertens. 34, 1309 (2016)
10. STAT3 Represses Nitric Oxide Synthesis in Human Macrophages upon Mycobacterium tuberculosis Infection: C.J. Queval, et al.; Sci. Rep. 6, 29297 (2016)
11. Flavonoids activate endothelial nitric oxide synthase by altering their phosphorylation via mitogen-activated protein kinase pathways in glucose-induced endothelial cells: C.E. Kim, et al.; J. Funct. Foods 17, 676 (2015)
12. Ginsenoside Rg3 regulates S-Nitrosylation of the NLRP3 inflammasome via suppression of iNOS: S. J. Yoon, et al.; Biochem. Biophys. Res. Commun. 463, 1184 (2015)
13. Propofol restores TRPV1 sensitivity via a TRPA1-, nitric oxide synthase-dependent activation of PKCε: P. Sinharoy, et al.; Pharmacol. Res. Perspect. 3, e00153 (2015)
14. Reduced flow-and acetylcholine-induced dilations in visceral compared to subcutaneous adipose arterioles in human morbid obesity: I. Grizelj, et al.; Microcirculation 22, 44 (2015)
15. Anti-inflammatory effects of Edaravone and Scutellarin in activated microglia in experimentally induced ischemia injury in rats and in BV-2 microglia: Y. Yuan, et al.; BMC Neurosci. 15, 125 (2014)
16. Lipopolysaccharide (LPS) stimulation of fungal secondary metabolism: Z.G. Khalil, et al.; Mycology 5, 168 (2014)
17. Cyclosporine attenuates arginine transport, in human endothelial cells, through modulation of cationic amino acid transporter-1: A. Grupper, et al.; Am. J. Nephrol. 37, 613 (2013)
18. Identification and characterization of a functional mitochondrial angiotensin system: P.M. Abadir, et al.; PNAS 108, 14849 (2011)
19. Loss of bcl-2 during the senescence exacerbates the impaired angiogenic functions in endothelial cells by deteriorating the mitochondrial redox state: M. Uraoka, et al.; Hypertension 58, 254 (2011)
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