CD21 is expressed strongly on mature B cells, follicular dentritic cells and weakly on immature thymocytes and T lymphocytes. In B-cell ontogeny, CD21 appears after the pre-B-stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells. CD21 expression is also gradually lost after stimulation of B cells in vitro. CD21 functions as receptor for C3d, C3dg and iC3b Complement components, for EBV and for IFNalpha. CD21 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B cell activation.
Clone
hCD21.
Immunogen
Purified human B-Cells.
Ig Subclass
Mouse IgG2a.
Purification Method
Protein-A.
Protein concentration
1mg/ml in PBS (after reconstitution).
Storage Procedures
Lyophilized: store at 4°C. After reconstitution, if not intended for use within a month, aliquot and store at -20°C.
Reconstitution
Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.
Antibody Properties
Staining antibody. For staining, use 10µl/1,000,000 cells.
Available Conjugates
This antibody is also available unconjugated and Biotin. For staining with biotin or FITC-conjugated antibody use 5-10µl/106 cells.
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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pAUR316是一种可以在构巢曲霉菌 (Aspergillus nidulans)中利用AMA1序列进行自主复制的穿梭载体。这个载体包含氨苄青霉素抗性基因 (作用于大肠杆菌)和AbA抗性基因aurAr (作用于A.nidulans)。限制酶BamH I, Aor51H I, Afl II, Bgl II 和 Sse8387 I可以作为克隆位点。
■ 保存
-20℃。
■ 链长
11,663 bp
■ 制备
CsCl-EtBr 超离心方法制备。
■ 用途
pAUR316是利用Aureobasidin A (AbA)进行构巢曲霉菌 (Aspergillus nidulans) 转化的载体。在构巢曲霉菌中以质粒状态存在。
[1]. Calabrese CR, et al. Anticancer chemosensitization and radiosensitization by the novel poly (ADP-ribose) polymerase-1 inhibitor AG14361. J Natl Cancer Inst. 2004 Jan 7; 96 (1) :56-67.
[2]. Vazquez-Ortiz G, et al. Drug repurposing screen identifies lestaurtinib amplifies the ability of the poly (ADP-ribose) polymerase 1 inhibitor AG14361 to kill breast cancer associated gene-1 mutant and wild type breast cancer cells. Breast Cancer Res. 2014 Jun 24; 16 (3) :R67.
[3]. Smith LM, et al. The novel poly (ADP-Ribose) polymerase inhibitor, AG14361, sensitizes cells to topoisomerase I poisons by increasing the persistence of DNA strand breaks. Clin Cancer Res. 2005 Dec 1; 11 (23) :8449-57.
备注
以上数据均来自公开文献, Jinpan暂未进行独立验证, 仅供参考。These protocols are for reference only. Jinpan does not independently validate these methods.