Thermo Scientific Pierce BSA Protein Assay Standards are high-quality reference samples for generating accurate standard curves and calibration controls in total protein assays.
These bovine serum albumin （BSA） solutions are protein concentration reference standards for use in BCA, Bradford and other protein assay protocols. BSA is the universally accepted reference protein for total protein quantitation. The albumin standard is precisely formulated at 2mg/mL in an ultrapure 0.9% sodium chloride （saline） solution. The concentration of the stock solution is calibrated by direct comparison to purified BSA （Fraction V） from the National Institute of Standards and Technology （NIST）. The product is available in three convenient package formats: 1mL glass ampules, 50mL polypropylene bottles, and complete sets containing seven ready-to-use dilutions.
Highlights:
Universal – recognized as the industry standard for protein quantitation in colorimetric protein assays
Pure and stable – supplied in ultrapure 0.9% saline solution with 0.05% sodium azide; room temperature stable
Accurate and consistent – precisely formulated at 2.00 +/-0.03mg/mL compared to an NIST reference
Convenient – choose 1mL ampules, a 50mL bottle, or a complete set containing seven ready-to-use dilutions
GS2‐M is a compley defined, serum‐free cell culture media formulation for the generation and long-term maintenance of human and mouse embryonic stem (ES) and induced pluripotent stem (iPS) cell lines. The medium contains two selective small molecule inhibitors that block differentiation‐inducing signals and promote cell survival.
GS2-M medium can be used in combination with the self-renewal cytokine leukemia inhibitory factor (LIF) to convert partial or pre‐iPS cells from mouse into fully pluripotent iPS cells via upregulation of Nanog (1, 2).
产品特点：
特点
优势
由Dr.Austin Smith研发
在很多杂志上发表，实验数据可信，可放心使用
成分确定、无血清
批次间一致，无需担心批次间差异
无饲养层培养
不需要准备饲养层细胞， 节省了准备饲养层细胞的时间和精力
使用GSK3β和 ERK/MEK抑制剂
维持细胞处于强的Ground state， 有效制备嵌合体小鼠，形成生殖系传递。
不添加LIF也可以发挥作用
组分简单，不需要额外购买LIF， 更易于准备完全培养基，节省了LIF的费用
Compley defined, serum-free formulation
Uses selective small molecule inhibitors to block differentiation signals from GSK3β and ERK/MEK and maintain ES and iPS cells in a pluripotent state
Allows long-term maintenance of germline-competent mouse and human ES and iPS cells
产品应用
小鼠部分或pre-iPS细胞转化为完全多能干细胞*
将人ES细胞或iPS细胞转化为初始(naïve)干细胞*
维持纯ES和iPS细胞的多能状态
*在Forskolin和/或LIF存在下
Conversion of partial or pre-iPS cells from mouse into fully pluripotent stem cells*
Conversion of human pre-ES or iPS cells into naïve stem cells*
Maintenance of pure ES and iPS cells in a pluripotent state
*In the presence of Forskolin and/or LIF
组份：
100 ml medium
100 µl 2i inhibitor supplement
自备试剂：
A. For mouse ES and iPS cells.
Culture vessels are pre-coated with either 0.1% gelatin type A in PBS solution (atroom temperature for 15 minutes or more), or precoated sequentially with 0.01%poly-L-ornithine hydrobromide (at 37℃ for 30 minutes or more), washing twicewith PBS, followed by 10 μg/mL laminin in PBS (at 37℃ for 3 hours or more).
B. For human iPS cells.
Please refer to Wang W, et al. (2011) reference for more detailed reagent and culture requirements to generate and propagate ‘naïve’ human iPS cells4.
保存：Upon receipt, store the media at -20℃ and the GS2-M 2i inhibitor supplementat -80℃ until ready to use. When stored under these conditions, the productsare stable for 12 months from the date of manufacture (see label).Once thawed and combined, store at 4℃ and use within 2 weeks.This product is light sensitive, and should be protected from light.
参考文献：
1. Silva J, et al. (2008) Promotion of reprogramming to ground state pluripo-tency by signal inhibition. PLoS Biol. 6: e253.
2.Silva J, et al. (2009) Nanog is the gateway to the pluripotent ground state.Cell. 138(4): 722-737.
3.Nichols J, et al. (2009) Validated germline-competent embryonic stem cell lines from non-obese diabetic mice. Nat Med. 15: 814-818.
4.Wang W, et al. (2011) Rapid and efficient reprogramming of somatic cells to induced pluripotent stem cells by retinoic acid receptor gamma and liver receptor homolog. PNAS (USA). 108(45): 18283-18288.
5.Reviewed in: Malaver-Ortega LF, et al. (2012) Theriogenology. 78(8): 1749-1762.
Western BLoT Immuno Booster包含增强抗原-抗体互作的组份。本制品适用于包括Western印迹和ELISA在内的各种免疫检测，可提高最终的检测灵敏度(从数倍到数十倍)。本制品不影响辣根过氧化物酶(HRP)或碱性磷酸酶(AP)活性，因此，兼容于交联二抗的酶和使用任何一种检测方法(如：比色检测法或发光检测法)。需要获得用于Western印迹的高检测信号时，请将本制品与Western BLoT HRP Substrate Series结合使用进行HRP化学发光检测。
