为了确定胱天蛋白酶-3在SLNT诱导的细胞凋亡中的作用，使用半胱天冬酶-3抑制剂（Ac-DEVD-CHO）。添加Ac-DEVD-CHO可显著预防SLNT诱导的细胞凋亡（从32.91±1.21％降至15.88±1.58％，而NC和Ac-DEVD-CHO组分别为6.45±0.96％，7.77±0.79％）[2] ]。经金丝桃素介导的PDT处理后，经zVAD-fmk（5.32％）或Ac-DEVD-CHO（7.43％）预处理的细胞凋亡率明显下降[3]。值得注意的是，10μmol/ L Ac-DEVD-CHO部分阻断了SIN诱导的细胞凋亡的作用并减少了凋亡细胞核的数量。 SIN的这些作用被胱天蛋白酶-3抑制剂Ac-DEVD-CHO阻断。喜树碱（4μM）是一种阳性对照，可增加caspase-3活性，这也被Ac-DEVD-CHO阻断[4]。
[1]. Garcia-Calvo M, et al.nhibition of human caspases by peptide-based and macromolecular inhibitors. J Biol Chem. 1998 Dec 4;273(49):32608-13.
[2]. Jinglin Wang, et al. A polysaccharide from Lentinus edodes inhibits human colon cancer cell proliferation and suppresses tumor growth in athymic nude mice. Oncotarget. 2017 Jan 3; 8(1): 610-623.
[3]. Junping Zhang, et al. Hypericin-mediated photodynamic therapy induces apoptosis of myoloma SP2/0 cells depended on caspase activity in vitro. Cancer Cell Int. 2015; 15: 58
[4]. Long-gang He, et al. Sinomenine induces apoptosis in RAW 264.7 cell-derived osteoclasts in vitro via caspase-3 activation. Acta Pharmacol Sin. 2014 Feb; 35(2): 203-210.
[5]. Liu LX, et al. The effect of caspase-3 inhibitor on the concentrations of serum inflammatory cytokines in sepsis related acute kidney injury induced by peritoneal cavity infection in mice. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2010 Dec;22(12):736-9
NIPI：无创产前筛查，是利用大规模平行测序等技术对母体外周血中的游离 DNA 进行深度测序，获取胎儿染色体信息的方法。传统的产前筛查和诊断方法( 孕早期超声检查、孕中期血清学筛查、羊水细胞染色体核型分析) 均有一定的局限性，尤其穿刺法可能会伤害胎儿导致流产。随着新一代测序技术研究的深入，无创性胎儿基因检测得到了快速发展。
提供产品：致力于为中国干细胞领域的干细胞科学研究、脐带血/造血干细胞/脐带/脂肪/胎盘干细胞库、干细胞临床转化医学、干细胞美容及干细胞抗衰老、免疫细胞治疗、T细胞治疗、临床干细胞治疗等再生医学和个体化治疗产业链的相关客户提供：MSC间充质干细胞培养基、iPS/ES胚胎干细胞培养基、血清替代物、羊水培养基、基底膜基质胶细胞支架、干细胞生长分化调控因子、BioMarker 流式检测抗体、支原体检测试剂盒、内毒素检测试剂盒、干细胞存储细胞冻存袋、细胞培养瓶、USP级DMSO二甲基亚砜细胞冻存液、细胞培养相关试剂耗材仪器等种类丰富的产品，以“Focus on improving the quality of Stem Cells专注干细胞研究，提升干细胞品质”为己任，我们与客户携手为中国干细胞产业的发展而前行。
Description :The plaet-derived growth factor (PDGF) family of heparin-binding growth factors consists of five known members, denoted PDGF-AA, PDGF-BB, PDGF-AB, PDGF-CC and PDGF-DD. The mature and active form of these proteins, an anti-parallel disulfide-linked dimer of two 12-14 kDa polypeptide chains, is obtained through proteolytic processing of biologically inactive precursor proteins, which contain an N-terminal CUB domain and a PDGF/VEGF homologous domain. The PDGFs interact with two related protein tyrosine kinase receptors, PDGFR-α and PDGFR-β, and are potent mitogens for a variety of cell types, including smooth muscle cells, connective tissue cells, bone and cartilage cells, and certain tumor cells. They play an important role in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubules epithelial cell development. Mature PDGFs are stored in plaet α-granules and are released upon plaet activation. PDGF-AA, -AB, -BB and –CC signal primarily through the PDGF-Rα receptor, whereas PDGF-DD interacts almost exclusively with the PDGF-Rβ receptor. Recombinant human PDGF-CC is a 25kDa protein consisting of two identical disulfide-linked 114 amino-acid polypeptide chains.
Catalog #:100-00CC
Source :E.coli
Purity :Greater than 98% by SDS-PAGE gel and HPLC analyses.
Endotoxin Level :Endotoxin level is less than 0.1 ng per μg (1EU/μg).
Biological Activity :Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected The ED 50 for this effect is 15-20 ng/ml.
Recombinant Protein 100-00AB 100-00AB-2 Human PDGF-AB 2ug Mini Recombinant Protein 100-00AB 100-00AB-10 Human PDGF-AB 10ug Standard Recombinant Protein 100-00AB 100-00AB-100 Human PDGF-AB 100ug Bulk Recombinant Protein 100-00AB 100-00AB-250 Human PDGF-AB 250ug Bulk Recombinant Protein 100-00AB 100-00AB-500 Human PDGF-AB 500ug Bulk Recombinant Protein 100-00AB 100-00AB-1000 Human PDGF-AB 1000ug Bulk Recombinant Protein 100-00CC 100-00CC-5 Human PDGF-CC 5ug Mini Recombinant Protein 100-00CC 100-00CC-20 Human PDGF-CC 20ug Standard Recombinant Protein 100-00CC 100-00CC-100 Human PDGF-CC 100ug Bulk Recombinant Protein 100-00CC 100-00CC-250 Human PDGF-CC 250ug Bulk Recombinant Protein 100-00CC 100-00CC-500 Human PDGF-CC 500ug Bulk Recombinant Protein 100-00CC 100-00CC-1000 Human PDGF-CC 1000ug Bulk Recombinant Protein 100-01 100-01-5 Human sCD22 5ug Mini Recombinant Protein 100-01 100-01-20 Human sCD22 20ug Standard Recombinant Protein 100-01 100-01-100 Human sCD22 100ug Bulk Recombinant Protein 100-01 100-01-250 Human sCD22 250ug Bulk Recombinant Protein 100-01 100-01-500 Human sCD22 500ug Bulk Recombinant Protein 100-01 100-01-1000 Human sCD22 1000ug Bulk Recombinant Protein 100-03 100-03-10 Human Heregulin beta-1 10ug Mini Recombinant Protein 100-03 100-03-50 Human Heregulin beta-1 50ug Standard Recombinant Protein 100-03 100-03-100 Human Heregulin beta-1 100ug Bulk Recombinant Protein 100-03 100-03-250 Human Heregulin beta-1 250ug Bulk Recombinant Protein 100-03 100-03-500 Human Heregulin beta-1 500ug Bulk Recombinant Protein 100-03 100-03-1000 Human Heregulin beta-1 1000ug Bulk Recombinant Protein 100-04 100-04-5 Human Epiregulin 5ug Mini Recombinant Protein 100-04 100-04-25 Human Epiregulin 25ug Standard Recombinant Protein 100-04 100-04-100 Human Epiregulin 100ug Bulk Recombinant Protein 100-04 100-04-250 Human Epiregulin 250ug Bulk Recombinant Protein 100-04 100-04-500 Human Epiregulin 500ug Bulk Recombinant Protein 100-04 100-04-1000 Human Epiregulin 1000ug Bulk Recombinant Protein 100-05 100-05-5 Human IGF Binding Protein-5 5ug Mini Recombinant Protein 100-05 100-05-25 Human IGF Binding Protein-5 25ug Standard Recombinant Protein 100-05 100-05-100 Human IGF Binding Protein-5 100ug Bulk Recombinant Protein 100-05 100-05-250 Human IGF Binding Protein-5 250ug Bulk Recombinant Protein 100-05 100-05-500 Human IGF Binding Protein-5 500ug Bulk Recombinant Protein 100-05 100-05-1000 Human IGF Binding Protein-5 1000ug Bulk Recombinant Protein 100-06 100-06-5 Human PLGF-1 5ug Mini Recombinant Protein 100-06 100-06-25 Human PLGF-1 25ug Standard Recombinant Protein 100-06 100-06-100 Human PLGF-1 100ug Bulk Recombinant Protein 100-06 100-06-250 Human PLGF-1 250ug Bulk Recombinant Protein 100-06 100-06-500 Human PLGF-1 500ug Bulk Recombinant Protein 100-06 100-06-1000 Human PLGF-1 1000ug Bulk Recombinant Protein 100-07 100-07-10 Human Prolactin 10ug Mini Recombinant Protein 100-07 100-07-50 Human Prolactin 50ug Standard Recombinant Protein 100-07 100-07-100 Human Prolactin 100ug Bulk Recombinant Protein 100-07 100-07-250 Human Prolactin 250ug Bulk Recombinant Protein 100-07 100-07-500 Human Prolactin 500ug Bulk Recombinant Protein 100-07 100-07-1000 Human Prolactin 1000ug Bulk Recombinant Protein 100-08 100-08-5 Human IGF Binding Protein-3 5ug Mini Recombinant Protein 100-08 100-08-25 Human IGF Binding Protein-3 25ug Standard Recombinant Protein 100-08 100-08-100 Human IGF Binding Protein-3 100ug Bulk Recombinant Protein 100-08 100-08-250 Human IGF Binding Protein-3 250ug Bulk Recombinant Protein 100-08 100-08-500 Human IGF Binding Protein-3 500ug Bulk Recombinant Protein 100-08 100-08-1000 Human IGF Binding Protein-3 1000ug Bulk Recombinant Protein 100-09 100-09-10 Human PTHrp 10ug Mini
[1]. Zeng X, et al. Pharmacologic inhibition of the anaphase-promoting complex induces a spindle checkpoint-dependent mitotic arrest in the absence of spindle damage. Cancer Cell. 2010 Oct 19;18(4):382-95. [2]. Zeng X, et al. An APC/C inhibitor stabilizes cyclin B1 by prematurely terminating ubiquitination. Nat Chem Biol. 2012 Feb 26;8(4):383-92. [3]. Ma Y, et al. Differential effects of Mg(ii) and N(alpha)-4-tosyl-l-arginine methyl ester hydrochloride on the recognition and catalysis in ATP hydrolysis. Dalton Trans. 2008 Feb 28;(8):1081-6.
规格
10mg 20mg
TAME hydrochloride 是一种活化蛋白 C (APC) 抑制剂，可通过作用于 Cdc20 和 Cdh1 来抑制 APC 的活性。
Recombinant Human G-CSF，中文名称：重组人粒细胞集落刺激因子，规格：10ug，货号：300-23
【详细说明】
Recombinant Human G-CSF:
G-CSF is a hematopoietic growth factor that stimulates the development of committed progenitor cells to neutrophils and enhances the functional activities of the mature end-cell. It is produced in response to specific stimulation by a variety of cells including macrophages, fibroblasts, endothelial cells and bone marrow stroma. G-CSF is being used clinically to facilitate hematopoietic recovery after bone marrow transplantation. Human and murine G-CSF are cross-species reactive. Recombinant human G-CSF is an 18.7 kDa protein consisting of 174 amino acid residues.
[1]. Tai X, et al. Cabazitaxel and indocyanine green co-delivery tumor-targeting nanoparticle for improved antitumor efficacy and minimized drug toxicity. J Drug Target. 2016 Sep 9:1-29.
[2]. Gdowski AS, et al. Bone-targeted cabazitaxel nanoparticles for metastatic prostate cancer skeletal lesions and pain. Nanomedicine (Lond). 2017 Sep;12(17):2083-2095.
