Epicypher热销产品——CTCF CUTANA™ CUT&RUN Antibody
EpiCypher是一家为表观遗传学和染色质生物学研究提供高质量试剂和工具的专业制造商。EpiCypher推出的CUT&RUN级别的CTCF Antibody符合EpiCypher的“CUTANA Compatible”标准，可用于核酸酶靶向切割和释放（CUT&RUN）和/或靶向剪切及转座酶技术（CUT&Tag）的基因组定位。CTCF通过结合DNA并阻止启动子和附近增强子或沉默子之间的相互作用来调节转录。
产品详情
反应种属: Human, Mouse
宿主来源: Rabbit
实验应用: CUT&RUN, IHC, ICC, IP, WB
免疫原: Between amino acids 650 and 700
克隆性: Monoclonal [BLR041F]
表达系统: HEK293 cells
保存温度: Stable for 1 year at 4°C from date of receipt
运输温度: DO NOT FREEZE!! Frozen cold packs.
产品形式: Purified recombinant monoclonal antibody in borate buffered saline pH 8.2, 0.1% BSA, 0.09% sodium azide
数据示例
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Figure 1: CTCF peaks in CUT&RUN
CUT&RUN was performed as described above. Peaks were called with MACS2. Heatmaps show CTCF peaks relative to IgG negative control antibody in aligned rows ranked by intensity (top to bottom) and colored such that red indicates high localized enrichment and blue denotes background signal. CTCF showed expected enrichment around the TSS. Despite some observable background in the IgG control, differential enrichment with the target antibody is as expected.
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Figure 2: CTCF CUT&RUN representative browser tracks
CUT&RUN was performed as described above. Gene browser shots were generated using the Integrative Genomics Viewer (IGV, Broad Institute). Three representative loci are shown.
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Figure 3: Immunohistochemistry data
FFPE sections of human colon carcinoma (left) and mouse spleen (right) using CTCF antibody at a dilution of 1:1000.
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Figure 4: Immunocytochemistry data
FFPE section of human MCF-7 cells examined using CTCF antibody at a dilution of 1:1,000.
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Figure 5: Immunoprecipitation data
EpiCypher CTCF antibody (6 μg/mg lysate) was used to immunoprecipitate whole cell lysates (1 mg, 20% of IP loaded) isolated from HEK293T cells. A negative control IgG antibody and positive control antibodies (Bethyl Laboratories) were also used to demonstrate specificity of the IP. All CTCF antibodies target the same CTCF epitope, however, A300-534A-6 is hosted in rabbit. For blotting immunoprecipitates, EpiCypher CTCF antibody was used at a 1:1,000 dilution.
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Figure 6: Western blot data
Western analysis of CTCF in whole cell extracts from HEK293T, MCF-7, Jurkat, Hep-G2, and A-549 cells. Fifty micrograms of lysate was resolved via SDS-PAGE and detected with a 1:1,000 dilution of CTCF antibody.
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订购详情
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货号
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产品名称
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规格
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13-2014
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CTCF CUTANA™ CUT&RUN Antibody
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100 µL
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如需了解更多详细信息或相关产品，请联系EpiCypher中国代理商-上海金畔生物
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数据精度。
