产品名称

RecombiMAb anti-mouse PD-1 (CD279) (D265A)

产品货号

CP005

产品规格

1mg

反应种属

Mouse

克隆号

29F.1A12™-CP005

同种型

Mouse IgG1(switched from rat IgG2a)

免疫原

Recombinant PD-1-Ig fusion protein

实验应用

in vivo blocking of PD-1/PD-L signaling*
in vitro PD-1 neutralization*
Immunohistochemistry (frozen)*
Immunofluorescence*
Western blot*
Flow cytometry*
*Reported for the original rat IgG2a 29F.1A12 antibody

产品形式

PBS, pH 7.0,Contains no stabilizers or preservatives

纯度

>95%, Determined by SDS-PAGE

聚合

<5%, Determined by SEC

无菌处理

0.2 µm filtration

纯化方式

Protein G

分子量

150 kDa

小鼠病原检测

Ectromelia/Mousepox Virus: Negative

Hantavirus: Negative

K Virus: Negative

Lactate Dehydrogenase-Elevating Virus: Negative

Lymphocytic Choriomeningitis virus: Negative

Mouse Adenovirus: Negative

Mouse Cytomegalovirus: Negative

Mouse Hepatitis Virus: Negative

Mouse Minute Virus: Negative

Mouse Norovirus: Negative

Mouse Parvovirus: Negative

Mouse Rotavirus: Negative

Mycoplasma Pulmonis: Negative

Pneumonia Virus of Mice: Negative

Polyoma Virus: Negative

Reovirus Screen: Negative

Sendai Virus: Negative

Theiler’s Murine Encephalomyelitis: Negative

保存条件

抗体原液保存在4°C，不能冷冻保存。

推荐抗体稀释液

InVivoPure pH 7.0 Dilution Buffer（货号IP0070）

应用

文章

体内PD-1/PD-L信号阻断

(in vivo blocking of 

PD-1/PD-L signaling)

1. Wang, W., et al. (2018). ‘RIP1 Kinase Drives Macrophage-Mediated Adaptive Immune Tolerance in 

Pancreatic Cancer’ Cancer Cell 34(5): 757-774 e757.

2. Gordon, S. R., et al. (2017). ‘PD-1 expression by tumour-associated macrophages inhibits phagocytosis 

and tumour immunity’ Nature 545(7655): 495-499.

3. Koyama, S., et al. (2016). ‘STK11/LKB1 Deficiency Promotes Neutrophil Recruitment and Proinflammatory 

Cytokine Production to Suppress T-cell Activity in the Lung Tumor Microenvironment’ Cancer Res 76(5): 

999-1008.

体内PD-1/PD-L信号阻断，

流式细胞术

(in vivo blocking of 

PD-1/PD-L signaling, 

Flow Cytometry)

1.Koyama, S., et al. (2016). ‘Adaptive resistance to therapeutic PD-1 blockade is associated with 

upregulation of alternative immune checkpoints’ Nat Commun 7: 10501.

体外PD-1中和

（in vitro PD-1 neutralization）

1.Park, S. J., et al. (2014). ‘Negative role of inducible PD-1 on survival of activated dendritic cells’ J 

Leukoc Biol 95(4): 621-629.

流式细胞术

（Flow Cytometry）

1.Good-Jacobson, K. L., et al. (2012). ‘CD80 expression on B cells regulates murine T follicular 

helper development, germinal center B cell survival, and plasma cell generation’ J Immunol 188(9): 

4217-4225.

免疫荧光，蛋白质印迹

(Immunofluorescence, 

Western Blot)

1.Chen, L., et al. (2009). ‘Role of the immune modulator programmed cell death-1 during development and 

apoptosis of mouse retinal ganglion cells’ Invest Ophthalmol Vis Sci 50(10): 4941-4948.