Hygromycin B 潮霉素B
货号：
IH0160
品牌：
Jinpan
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产品简介
| MDL | MFCD00036846 |
| EC | EINECS 250-545-5 |
| 别名 | Destomycin A; Hygrovetin |
| CAS | 31282-04-9 |
| 分子式 | C20H37N3O13 |
| 分子量 | 527.52 |
| 纯度 | ≥90% |
| 单位 | 瓶 |
| 生物活性 | Hygromycin B is an aminoglycoside antibiotic active against prokaryotic and eukaryotic cells.[1-3] |
| In Vitro | Hygromycin B, an aminocyclitol antibiotic that strongly inhibits both 70S and 80S ribosomes, is synthesized by Streptomyces hygroscopicus[1]. Hygromycin B at 0.38 mM concentration completely halts yeast cell growth in rich media, presumably by preventing protein synthesis by cytoplasmic ribosomes. Polypeptide synthesis in cell-free extracts from rabbit reticulocytes, wheat germ and yeast is strongly blocked by low concentrations ofhygromycin B. The antibiotic inhibits peptide chain elongation by yeast polysomes by preventing elongation factor EF-2-dependent translocation. The inhibition of translocation by hygromycin B might result from the stabilization of peptidyl-tRNA bound to the ribosomal acceptor site[2]. |
| In Vivo | Hygromycin B inhibits protein synthesis by blocking ribosomal translocation without causing significant misreading in vivo[3]. Constitutive expression of the bacterial hygR gene in transgenic mice in vivo confers resistance to hygromycin B[4]. |
| SMILES | O[C@H]([C@H]1O)C2(O[C@@]([C@@H](N)CO)([H])[C@@H]1O)O[C@@]([C@@H](O[C@@H]3CO)O[C@@]([C@H]([C@H](N)C4)O)([H])[C@@H]([C@H]4NC)O)([H])[C@@]([C@H]3O)([H])O2 |
| 靶点 | Bacterial;Fungal |
| 动物实验 | Hygromycin B is dissolved in sterile water. The mice C57BL/6J-TgN(pPWL512hyg)1Ems carrying hygR are treated with a single dose of hygromycin B i.p. at doses that starts at 2.7 mg/kg and increases by 50% for each consecutive dose. Control wild-type C57BL/6J mice are treated with the same volume of sterile saline. Total volume injected is 0.5 mL. The health status and body weights of animals are monitored daily for 10 consecutive days[4]. |
| 数据来源文献 | [1]. Pardo JM, et al. Biochemical basis of resistance to hygromycin B in Streptomyces hygroscopicus–the producing organism. J Gen Microbiol. 1985 Jun;131(6):1289-98.
[2]. González A, et al. Studies on the mode of action of hygromycin B, an inhibitor of translocation in eukaryotes. Biochim Biophys Acta. 1978 Dec 21;521(2):459-69. [3]. Pfister P, et al. Role of 16S rRNA Helix 44 in Ribosomal Resistance to Hygromycin B. Antimicrob Agents Chemother. 2003 May;47(5):1496-502. |
| 规格 | 200mg 10mM*1mL (in Water) 1g |
是抑制原核和真核细胞的氨基糖苷类抗生素，通过干扰移位、促进70S核糖体的误译来抑制蛋白质合成。