亮抑酶肽
| MDL | MFCD00037012 |
| EC | EINECS 600-443-5 |
| 别名 | 亮抑蛋白酶肽;LeupeptinHemisulfate |
| 英文名称 | Leupeptin |
| CAS | 103476-89-7 |
| 分子式 | C20H38N6O4·1/2 H2SO4 |
| 分子量 | 475.59 |
| 纯度 | ≥98% |
| 单位 | 瓶 |
| SMILES | O=C[C@@H](NC([C@@H](NC([C@@H](NC(C)=O)CC(C)C)=O)CC(C)C)=O)CCCNC(N)=N.[0.5H2SO4] |
| 靶点 | Cathepsin;Serine Protease |
| 规格 | 1mg 5mg 10mM*1mL (in DMSO) 10mM*1mL in Water |
是一种可透过膜的巯基蛋白酶抑制剂，可抑制 Cathepsin B, Cathepsin H 和 Cathepsin L，并损害两性溶酶体的融合。
使用本产品的应用案例（仅供参考）
In Vitro
Western blotting. （1 µM Leupeptin）
MCF-7 cells were treated with the aforementioned CoCl2 concentrations for 24 h and cells were lysed with ice-cold radioimmunoprecipitation assay buffer (150 mM NaCl, 1.0% NP-40, 0.1% Triton X-100, 0.5% sodium deoxycholate, 0.1% SDS and 50 mM Tris-HCl, pH 8.0) and protease inhibitors (AEBSF at 2 mM, Aprotinin at 0.3 µM, Bestatin at 116 µM, E-64 at 14 µM, Leupeptin at 1 µM and EDTA at 1 mM; Beijing Jinpan Science & Technology Co., Ltd., Beijing, China).
来源文献：Li Q, Ma R, Zhang M. CoCl2 increases the expression of hypoxic markers HIF-1α, VEGF and CXCR4 in breast cancer MCF-7 cells. Oncol Lett. 2018 Jan;15(1):1119-1124. doi: 10.3892/ol.2017.7369. Epub 2017 Nov 8. PMID: 29391899; PMCID: PMC5769392.