Millipore超滤管 15ml 10K 产品信息:
货号: UFC901096
产品名称: Millipore超滤管 15ml 10K
品牌: Millipore
规格: 15ml 10K
Amicon Ultra-4* and -15* Centrifugal Filter Units are designed for in vitro diagnostic use and intended for concentrating serum, urine, cerebrospinal fluid and other body fluids prior to analysis.
The premier devices for concentrating 1 – 4mL or <15 mL
High recovery Ultracel regenerated cellulose membrane in a range of molecular weight cut-offs
High retentate recovery of >90%
Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10–15 minutes)
Heat-sealed membrane minimizes downstream extractables
100% integrity tested for reliable performance
Convenient sample monitoring with translucent housing and volume gradations
Direct pipettor sample access eliminates processing step to recover concentrate
High concentration factors of 80–100X
* The Amicon Ultra-4 and Amicon Ultra-15 with Ultracel-10 membrane are CE marked.
Applications
Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
Purification of macromolecular components found in tissue culture extracts or cell lysates
Concentration of dilute or pre-purified proteins from column eluents
Desalting and buffer exchange
Performance data – Amicon Ultra-4 Centrifugal Filter Unit
Typical Spin Times to Filtrate Volume
Spin conditions: 4000 xg swinging bucket rotor at 25 ° C, 4 mL sample. 3K, 10K: Cytochrome c (0.25 mg/mL); 30K, 50K: BSA (1 mg/mL); 100K: IgG (1 mg/mL).
Typical Protein Recovery
Membrane NMWL, kDa
Protein Solute
Protein Retentate Volume, mL
Protein Retentate Recovery, %
10
Cytochrome c, 12.4 kDa (0.25 mg/mL)
0.20
95
4 mL starting volume in a fixed angle rotor at 7,500 x g at 25 °C. Spin times: 3K (30 min); 10 and 30K (10 min); 50K (5 min); 100K (15 min).
Performance data – Amicon Ultra-15 Centrifugal Filter Unit
Typical Spin Times to Filtrate Volume
Spin conditions: 4000 xg swinging bucket rotor at 25° C, 15 mL sample. 3K, 10K: Cytochrome c (0.25 mg/mL); 30K, 50K: BSA (1 mg/mL); 100K: IgG (1 mg/mL).
Typical Protein Recovery
Membrane NMWL, kDa
Protein Solute
Protein Retentate Volume, mL
Protein Retentate Recovery, %
10
Cytochrome c, 12.4 kDa (0.25 mg/mL)
0.24
93.03
15 mL starting volume in swinging bucket rotor at 4000 x g at 25 °C. Spin times: 3K (40 min); 10K and 100K (20 min); 30K (10 min); 50K (15 min).
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