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SuperSignal (R) West Femto Maximum Sensitivity Substrate 介绍
Thermo Scientific BCA蛋白定量产品可以用于研究蛋白:蛋白间相互作用,衡量亲和层析后的组分含量,估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。
SuperSignal (R) West Femto Maximum Sensitivity Substrate 现货供应
PIERCE
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34096
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SuperSignal (R) West Femto Maximum Sensitivity Substrate
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200 ML
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PIERCE
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34150
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Lumi-Phos ™ WB, 100 mL.
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100 ML
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PIERCE
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35035
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Pierce Fast Semi-Dry Transfer Buffer, 10X Methanol-free
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SuperSignal (R) West Femto Maximum Sensitivity Substrate 品牌介绍
美国Pierce公司(www.pierce.com)是蛋白质化学、免疫学和层析等很多方面的先驱和领导者。http://www.thermo.com.cn/Category828.html
主要产品包括:
1)化学发光体和比色底物
2)分析试剂
3)荧光标记探针
4)酶联免疫分析试剂盒等
SuperSignal (R) West Femto Maximum Sensitivity Substrate 仅用于科研
Thermo Scientific SuperSignal West Femto Substrate is an ultra-sensitive enhanced chemiluminescent substrate (ECL substrate) for detection of peroxidase activity from HRP-conjugated antibodies and probes in Western blotting.
SuperSignal West Femto Substrate Maximum Sensitivity Substrate is an ultra-sensitive chemiluminescent substrate system for Western blotting with horseradish peroxidase (HRP) enzyme. The substrate kit enables detection of low femtogram (that’s mid-zeptomole!) amounts of protein deposited on nitrocellulose or PVDF membrane and probed with appropriate primary and secondary antibodies. When combined with optimized antibody concentrations and blocking buffers, SuperSignal West Femto Substrate enables detection of target proteins in amounts that are small to be seen with typical ECL substrates.
Highlights:
- ECL substrate – an enhanced chemiluminescent substrate for detection of horseradish peroxidase (HRP) activity from antibodies and other Western blot probes
- Sensitive – detect low-femtogram (mid-zeptomole) amounts of protein in bands on nitrocellulose or PVDF membranes
- Quantitative – produces quantitative signal that is measurable over two orders of magnitude
- Saves on antibody – optimized for very dilute antibody concentrations; dilute primary antibodies 5000- to 100,000-fold and secondary antibodies 100,000 to 500,000-fold (from 1mg/mL stocks)
- Intense signal – easy to capture an image by exposure to autoradiography film or via a CCD camera or similar imaging system
- Excellent signal duration – 8 hours of usable light-output when conditions are optimized
- Stable reagent – kit components are stable for 1 year at 4°C or 6 months at room temperature
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