货号：22122，名称：Sulfo-SMCC, 1 g.，品牌：PIERCE，规格：1 G

订货：，，

：  787994648

Sulfo-SMCC介绍

Thermo Scientific BCA蛋白定量产品可以用于研究蛋白：蛋白间相互作用，衡量亲和层析后的组分含量，估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。

Sulfo-SMCC现货供应

Sulfo-SMCC品牌介绍

美国Pierce公司（www.pierce.com）是蛋白质化学、免疫学和层析等很多方面的先驱和领导者。http://www.thermo.com.cn/Category828.html

主要产品包括：

1）化学发光体和比色底物

2）分析试剂

3）荧光标记探针

4）酶联免疫分析试剂盒等

Sulfo-SMCC仅用于科研

Thermo Scientific Pierce Sulfo-SMCC is a very popular, water-soluble, amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane spacer arm （8.3 angstroms）.

Sulfosuccinimidyl-4-（N-maleimidomethyl）cyclohexane-1-carboxylate （Sulfo-SMCC） is a water-soluble, non-cleavable and membrane impermeable crosslinker. It contains an amine-reactive N-hydroxysuccinimide （NHS ester） and a sulfhydryl-reactive maleimide group. NHS esters react with primary amines at pH 7-9 to form stable amide bonds. Maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. The maleimide groups of Sulfo-SMCC and SMCC are unusually stable up to pH 7.5 because of the cyclohexane bridge in the spacer arm. Because it contains the hydrophilic sulfonyl moiety, Sulfo-SMCC is soluble up to ~10 mM in water and many commonly used buffers, thus avoiding the use of organic solvents which may perturb protein structure.

Sulfo-SMCC Highlights

• Amine reactive Sulfo-NHS ester crosslinks rapidly with primary amine-containing molecule
• Sulfhydryl-reactive maleimide reacts with cysteine residues to yield specific conjugates
• Sulfo-SMCC is water soluble, so crosslinking can be done in physiologic solutions
• High purity, crystalline Sulfo-SMCC can be used to create high-purity maleimide-activated derivatives
• Cyclohexane bridge confers added stability to the maleimide group making Sulfo-SMCC the ideal crosslinking agent for maleimide activation of proteins. Maleimide groups are stable for 64 hours in 0.1 M sodium phosphate buffer, pH 7 at 4°C。