Anti-ZO1 tight junction protein antibody (ab59720)抗ZO1紧密连接蛋白抗体
货号：ab59720
厂家：Abcam公司
规格：50ug
保存温度：-20℃ 和 2～8℃
货期：现货2～5天，期货2-3周
咨询： （重庆） （贵阳）
Anti-ZO1 tight junction protein antibody (ab59720)抗ZO1紧密连接蛋白抗体产品信息：
- Product name：Anti-ZO1 tight junction protein antibodySee all ZO1 tight junction protein primary antibodies …
- Description
Rabbit polyclonal to ZO1 tight junction protein
- Specificityab59720 recognises both ZO1 tight junction alpha-minus and alpha-plus isoforms. The protein is abundant in endothelial cells and the highly specialized epithelial junctions. Although we have an image for IHC-Fr, customers report variable results in this application. If you would like to use in IHC-Fr then we suggest using ethanol instead of acetone. Although we have references for IHC-P, customers report variable results in this application. IHC-Fr and IHC-P are therefore not covered by our Abpromise guarantee.
- Tested applicationsICC/IF, Flow Cyt, IHC (PFA fixed), WB, IHC-P more details
- Species reactivity
Reacts with: Mouse, Chicken, Dog, Human, Pig
- Immunogen
Corresponding to amino acids 432-1150 of Human ZO1 tight junction protein
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.1% BSA, PBS -
Concentration 50 µg at 0.1 mg/ml
- Purification notes0.2µm filtered.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
- Tags & Cell Markers
- Subcellular Markers
- Plasma Membrane
Applications
Our Abpromise guarantee covers the use of ab59720 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | ICC/IF: Use a concentration of 1 µg/ml. |
| Flow Cyt | Flow Cyt: 1/50. |
| IHC (PFA fixed) | IHC (PFA fixed): Use at an assay dependent concentration. PubMed: 21526120 |
| WB | WB: 1/50. Predicted molecular weight: 194 kDa. |
| IHC-P | IHC-P: Use at an assay dependent concentration. |
Target
- FunctionThe N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions.
- Tissue specificityThe alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.
- Sequence similaritiesBelongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain.
Contains 1 ZU5 domain. - DomainThe second PDZ domain mediates interaction with GJA12.
- Post-translational
modificationsPhosphorylated. Dephosphorylated by PTPRJ. - Cellular localizationCell membrane. Cell junction > tight junction. Movement of ZO-1 from the cytoplasm to membrane is an early event occurring concurrently with cell-cell contact.
- Information by UniProt
-
Database links
- Entrez Gene: 7082 Human
- Entrez Gene: 21872 Mouse
- Omim: 601009 Human
- SwissProt: Q07157 Human
- SwissProt: P39447 Mouse
- Unigene: 510833 Human
- Unigene: 4342 Mouse
-
Alternative names
- Tight junction protein 1 antibody
- Tight junction protein ZO-1 antibody
- Tight junction protein ZO1 antibody
Anti-ZO1 tight junction protein antibody images
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Immunocytochemistry/ Immunofluorescence – Anti-ZO1 tight junction protein antibody (ab59720)
ab59720 staining ZO1 in Caco-2 cells treated with (±)-palmitoylcarnitine chloride (ab141122), by ICC/IF. Membranar ZO1 expression loss correlates with increased concentration of (±)-palmitoylcarnitine chloride, as described in literature.
The cells were incubated at 37°C for 1 hour in media containing different concentrations of ab141122 ((±)-palmitoylcarnitine chloride) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab59720 (0.5 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue. 
Immunocytochemistry/ Immunofluorescence – ZO1 tight junction protein antibody (ab59720)
ICC/IF image of ab59720 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59720, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunocytochemistry/ Immunofluorescence – Anti-ZO1 tight junction protein antibody (ab59720)This image is courtesy of an anonymous Abreview
Immunofluorescence analysis of HUVEC cells, staining ZO1 tight junction protein with ab59720.
Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200 in diluent) for 16 hours at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence – Anti-ZO1 tight junction protein antibody (ab59720)Image from Kokkinopoulos I et al., PLoS One. 2011 Apr 22;6(4):e18921. Fig 6.; doi:10.1371/journal.pone.0018921; April 22, 2011, PLoS ONE 6(4): e18921.
Immunofluorescence analysis of murine peripheral retinal pigmented epithelium (RPE) cells, staining ZO1 tight junction protein with ab59720.
Cells were fixed with 4% paraformaldehyde for 10 minutes at room temperature. Cells were blocked in 10% BSA and 0.5% Triton-X 100 for 2 hours at room temperature before being incubated with primary antibody (1/100) in blocking solution overnight at room temperature. An AlexaFluor®-conjugated anti-rabbit IgG was used as the secondary antibody.
公司介绍：
Abcam位于英国的剑桥科学园，成立于1998年，专门生产和分销研究型抗体。主要创立人Jonathan Milner和实验室同事构思成立一间了解科研人员需要的网上抗体公司，理念是Abcam销售质量高的抗体，并提供zui全面、诚实、时新的说明书，快速的送货服务，及高效率的顾客和服务。我们的在线目录(www.abcam.cn) 已有差不多100,000种抗体和试剂，并不断添加，供应予全球百多个国家。Abcam于2005年11月在伦敦证券交易所上市，在美国马萨诸塞州、日本东京及香港均设有分公司。
Abcam 一直致力加强产品线，为使研究员更容易找到蛋白质研究试剂产品，在2011并购了美国的MitoSciences公司，加强了免疫分析方面的产品供应；同年也并购了英国的Ascent Scientific 公司，开展了生化试剂的供应。在2012年，Abcam并购了美国的Epitomics 公司，成为一家有领导地位的RabMAbs® 供应商。
Abcam 的 目标是给世界上zui好的抗体建立zui大的在线目录，为各地科学家提供尖端产品，成为各国科学界的重要伙伴。我们为所有产品提供来使客户获得预期的结 果。为提供高质量的抗体来指向尽可能多的靶蛋白，我们尽所能在尽可能多的应用和物种中检测每种抗体。我们相信诚信才是上策，在我们的上会发布有关我们 每一种产品的尽可能多的信息。
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