Anti-USP13 antibody (ab71734)抗泛素特异性蛋白酶USP13抗体
货号：ab71734
厂家：Abcam公司
规格：100ug
保存温度：-20℃ 和 2～8℃
货期：现货2～5天，期货2-3周
咨询： （重庆） （贵阳）
Anti-USP13 antibody (ab71734)抗泛素特异性蛋白酶USP13抗体产品信息：
- Product nameAnti-USP13 antibodySee all USP13 primary antibodies …
- Description
Rabbit polyclonal to USP13
- Tested applicationsELISA, IHC-P, WB more details
- Species reactivity
Reacts with: Mouse, Human
- Immunogen
KLH conjugated synthetic peptide selected from the center region of human USP13.
- Positive control
- HeLa cell lysate, mouse brain tissue lysate and human breast cancer tissue
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: PBS -
Concentration 100 µg at 0.25 mg/ml
- PurityProtein G purified
- Purification notesab71734 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediay, followed by dialysis against PBS.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
- Epigenetics and Nuclear Signaling
- Chromatin Modifying Enzymes
- Ubiquitylation
- Cell Biology
- Proteolysis / Ubiquitin
- Proteasome / Ubiquitin
- Deubiquitination
- Epigenetics and Nuclear Signaling
- Ubiquitin & Ubiquitin Like Modifiers
- Deubiquitination
Applications
Our Abpromise guarantee covers the use of ab71734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | ELISA: 1/1000. |
| IHC-P | IHC-P: 1/50 – 1/100. |
| WB | WB: 1/100 – 1/500. Detects a band of approximay 100 kDa (predicted molecular weight: 97 kDa). |
Target
- Tissue specificityHighly expressed in ovary and testes.
- Sequence similaritiesBelongs to the peptidase C19 family.
Contains 2 UBA domains.
Contains 1 UBP-type zinc finger. - Information by UniProt
-
Database links
- Entrez Gene: 8975 Human
- Entrez Gene: 72607 Mouse
- Omim: 603591 Human
- SwissProt: Q92995 Human
- SwissProt: Q5BKP2 Mouse
- Unigene: 175322 Human
- Unigene: 316153 Mouse
-
Alternative names
- Deubiquitinating enzyme 13 antibody
- Isopeptidase T 3 antibody
- Isopeptidase T-3 antibody
see all
Anti-USP13 antibody images
Western blot – USP13 antibody (ab71734)
All lanes : Anti-USP13 antibody (ab71734) at 1/100 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Mouse brain tissue lysate
Lysates/proteins at 12.5 µg per lane.
Predicted band size : 97 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) – USP13 antibody (ab71734)
ab71734, at a 1/50 dilution, staining USP13 in formalin fixed, paraffin embedded human breast carcinoma tissue sections by Immunohistochemistry. ab71734 was peroxidase conjugated to the secondary antibody, followed by DAB staining.
公司介绍：
Abcam位于英国的剑桥科学园，成立于1998年，专门生产和分销研究型抗体。主要创立人Jonathan Milner和实验室同事构思成立一间了解科研人员需要的网上抗体公司，理念是Abcam销售质量高的抗体，并提供zui全面、诚实、时新的说明书，快速的送货服务，及高效率的顾客和服务。我们的在线目录(www.hystemcell.cn) 已有差不多100,000种抗体和试剂，并不断添加，供应予全球百多个国家。Abcam于2005年11月在伦敦证券交易所上市，在美国马萨诸塞州、日本东京及香港均设有分公司。
Abcam 一直致力加强产品线，为使研究员更容易找到蛋白质研究试剂产品，在2011并购了美国的MitoSciences公司，加强了免疫分析方面的产品供应；同年也并购了英国的Ascent Scientific 公司，开展了生化试剂的供应。在2012年，Abcam并购了美国的Epitomics 公司，成为一家有领导地位的RabMAbs® 供应商。
Abcam 的 目标是给世界上zui好的抗体建立zui大的在线目录，为各地科学家提供尖端产品，成为各国科学界的重要伙伴。我们为所有产品提供来使客户获得预期的结 果。为提供高质量的抗体来指向尽可能多的靶蛋白，我们尽所能在尽可能多的应用和物种中检测每种抗体。我们相信诚信才是上策，在我们的上会发布有关我们 每一种产品的尽可能多的信息。
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