Anti-Vimentin antibody [2D1] (ab125089)抗波形蛋白抗体
货号：ab125089
厂家：Abcam公司
规格：100ul
保存温度：-20℃ 和 2～8℃
货期：现货2～5天，期货2-3周
咨询： （重庆） （贵阳）
Anti-Vimentin antibody [2D1] (ab125089)抗波形蛋白抗体产品信息：
- Product nameAnti-Vimentin antibody [2D1]
Mouse monoclonal [2D1] to Vimentin
- Tested applicationsWB, ICC/IF, Flow Cyt
- Species reactivity
Reacts with: Mouse, Rat, Cow, Human, Pig
- Immunogen
Recombinant full length purified protein corresponding to Human Vimentin expressed in E.coli.
- Positive control
- Neuron/glial cultures HeLa cell line lysate
Properties
- FormLiquid
- Storage instructionsStore at 4°C or at -20°C for long term storage.
- Storage bufferPreservative: 0.06% Sodium azide
Constituent: 99% PBS -
Concentration 100 µl at 1 mg/ml
- PurityIgG fraction
- Clonality Monoclonal
- Clone number2D1
- IsotypeIgG2a
- Light chain typekappa
Applications
Our Abpromise guarantee covers the use of ab125089 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/10000. Predicted molecular weight: 54 kDa. |
| ICC/IF | ICC/IF: 1/1000. |
| Flow Cyt | Flow Cyt: Use 0.1µg for 106 cells. |
Target
- FunctionVimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. - Tissue specificityHighly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
- Sequence similaritiesBelongs to the intermediate filament family.
- DomainThe central alpha-helical coiled-coil rod region mediates elementary homodimerization.
- Post-translational
modificationsFilament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33. - Cellular localizationCytoplasm.
-
Database links
- Entrez Gene: 280955 Cow
- Entrez Gene: 7431 Human
- Entrez Gene: 22352 Mouse
- Entrez Gene: 407747 Pig
- Entrez Gene: 81818 Rat
- Omim: 193060 Human
- SwissProt: P48616 Cow
- SwissProt: P08670 Human
- FormVimentin is found in connective tissue and in the cytoskeleton.
-
Alternative names
- FLJ36605 antibody
- VIM antibody
- VIME_HUMAN antibody
- Vimentin antibody
Anti-Vimentin antibody [2D1] images
-
Flow Cytometry – Anti-Vimentin antibody [2D1] (ab125089)
Overlay histogram showing HeLa cells stained with ab125089 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab125089, 0.1μg/1×106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1×106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Immunocytochemistry/ Immunofluorescence – Anti-Vimentin antibody [2D1] (ab125089)
ab125089, at 1/1000, staining Vimentin in mixed neuron/glial cultures by Immunofluorescence (green).
Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and vimentin, the two protein assemble to produce heteropolymer filaments
Western blot – Anti-Vimentin antibody [2D1] (ab125089)
Anti-Vimentin antibody [2D1] (ab125089) at 1/10000 dilution + HeLa cell line lysate
developed using the ECL techniquePredicted band size : 54 kDa
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Abcamab1150Rabbit polyclonal to IKAP100ul3996IKAPhystemcell一抗
Abcamab11501Rabbit polyclonal to Nephronophthisis50ug3996Nephronophthisishystemcell一抗
Abcamab11503Goat polyclonal to Osteopontin25ug3996Osteopontinhystemcell一抗
Abcamab11504Rat monoclonal [KT12] to TCR V beta 12 2ml3996TCR V beta 12hystemcell一抗
Abcamab11505Mouse monoclonal [G3] to CD3 zeta （FITC）500ul3996TCR zeta chainhystemcell一抗
Abcamab11506Mouse monoclonal [15F11] to gamma Catenin100ul3996gamma Cateninhystemcell一抗
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