Anti-SATB2 antibody 抗SATB2抗体
货号：ab34735
厂家：Abcam公司
规格：100ul
保存温度：-20℃ 和 2～8℃
货期：现货2～5天，期货2-3周
咨询： （重庆） （贵阳）
Anti-SATB2 antibody 抗SATB2抗体产品信息：
- Product nameAnti-SATB2 antibody
- Description
Rabbit polyclonal to SATB2
- Tested applicationsWB, EMSA, IHC-FoFr, ICC/IF
- Species reactivity
Reacts with: Mouse, Rat, Human
- Immunogen
Synthetic peptide conjugated to KLH derived from within residues 700 to the C-terminus of Mouse SATB2.
Read Abcam's proprietary immunogen policy
(Peptide available as ab34906.)
- Positive control
- Brain (Mouse) Whole Cell Lysate – normal tissue, 0 days old.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4 -
Concentration 100 µg at 1 mg/ml
- PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
Applications
Our Abpromise guarantee covers the use of ab34735 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 1 µg/ml. Detects a band of approximay 85 kDa (predicted molecular weight: 83 kDa).Can be blocked with Mouse SATB2 peptide (ab34906). |
| EMSA | EMSA: Use at an assay dependent concentration. PubMed: 22123820 |
| IHC-FoFr | IHC-FoFr: Use at an assay dependent dilution. |
| ICC/IF | ICC/IF: Use at an assay dependent concentration. PubMed: 19232401 |
Target
- FunctionBinds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Required for the initiation of the upper-layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating BCL11B expression. Repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. May play an important role in palate formation. Acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation.
- Tissue specificityHigh expression in adult brain, moderate expression in fetal brain, and weak expression in adult liver, kidney, and spinal cord and in select brain regions, including amygdala, corpus callosum, caudate nucleus, and hippocampus.
- Involvement in diseaseNote=Chromosomal aberrations involving SATB2 are found in isolated cleft palate. Translocation t(2;7); translocation t(2;11).
Defects in SATB2 are a cause of cleft palate isolated (CPI) [MIM:119540]. A congenital fissure of the soft and/or hard palate, due to faulty fusion. Isolated cleft palate is not associated with cleft lips. Some patients may manifest other craniofacial dysmorphic features, mental retardation, and osteoporosis.
Note=A chromosomal aberration involving SATB2 is found in a patient with classical features of Toriello-Carey syndrome. Translocation t(2;14)(q33;q22). - Sequence similaritiesBelongs to the CUT homeobox family.
Contains 2 CUT DNA-binding domains.
Contains 1 homeobox DNA-binding domain. - Post-translational
modificationsSumoylated by PIAS1. Sumoylation promotes nuclear localization, but represses transcription factor activity. - Cellular localizationNucleus matrix.
-
Alternative names
- DNA binding protein SATB2 antibody
- DNA-binding protein SATB2 antibody
- FLJ21474 antibody
see all
Anti-SATB2 antibody images
Western blot – SATB2 antibody (ab34735)
Anti-SATB2 antibody (ab34735) at 1 µg/ml + Brain (Mouse) Tissue Lysate – normal tissue, 0 days old (ab7188) at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilutionPerformed under reducing conditions.
Predicted band size : 83 kDa
Observed band size : 85 kDa (why is the actual band size different from the predicted?)
Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
Electrophoretic Mobility Shift Assay – Anti-SATB2 antibody (ab34735)Image from Asanoma K et al, J Biol Chem. 2012 Jan 13;287(3):2257-68. Epub 2011 Nov 28, Fig 8. DOI 10.1074/jbc.M111.287128 January 13, 2012 The Journal of Biological Chemistry, 287, 2257-2268.
ab34735 used in EMSA.
EMSA demonstrating binding of SATB1 and SATB2 to an oligonucleotide containing a predicted SATB-binding element located 125 bp upstream of the Eomes transcription start site (-125). Labeled SATB probes were incubated with Rcho-1 TS cell nuclear lysates as indicated above the lanes. Lane 1, without any competitor oligonucleotide; lane 2, with unlabeled wild-type competitor; lane 3, with unlabeled mutant competitor; lane 4, with SATB1 antibody; lane 5, with SATB2 antibody (ab34735); lane 6, with a control antibody. B and C, Rcho-1 TS cells stably transfected with pcDNA3, pcDNA3-HA-Satb1, or pcDNA3-HA-Satb2 and maintained in stem culture conditions were used for ChIP assays. Antibodies to the HA tag (Anti-HA) or acetylated histone H3K9 (Anti-AcH3) were used in the analyses. IgG was used as a control for nonspecific immunoprecipitation. qPCR analyses were performed on immunoprecipitates using primers amplifying the segment (-172 to -63) including the SATB-binding element at -125.
Immunocytochemistry/ Immunofluorescence – Anti-SATB2 antibody (ab34735)Image courtesy of an anonymous Abreview.
ab34735 staining SATB2 in human osteosarcoma SAOS-2 cells by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in paraformaldehyde, permeabilized using PBS/ 0.25% Triton, blocked with 1% BSA for 1 hour at room temperature and then incubated with ab34735 at a 1/500 dilution for 1 hour. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/250 dilution.
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Abcamab11343Rabbit polyclonal to CASK100ul3996CASKhystemcell一抗
Abcamab11346Rabbit polyclonal to alpha 1 Catenin100ul3885alpha 1 Cateninhystemcell一抗
Abcamab11347Rabbit polyclonal to alpha 2 Catenin100ul3996alpha 2 Cateninhystemcell一抗
Abcamab11348Mouse monoclonal [15B8] to beta Catenin （Cy3 ®）100ul3996beta Cateninhystemcell一抗
Abcamab11349Mouse monoclonal [BC-76] to beta Catenin （phospho S33）50ul3996beta Cateninhystemcell一抗
Abcamab1135Rabbit polyclonal to GABA B Receptor 1100ul3996GABA B Receptor 1hystemcell一抗
Abcamab11350Mouse monoclonal [BC-22] to beta Catenin （phospho S33+S37）50ul3996beta Cateninhystemcell一抗
Abcamab11351Rabbit polyclonal to delta 2 Catenin100ul3996delta 2 Cateninhystemcell一抗
Abcamab11352Rabbit polyclonal to delta 2 Catenin100ul3996delta 2 Cateninhystemcell一抗
Abcamab11354Mouse monoclonal [4C3] to HMGB150ug3996HMGB1hystemcell一抗
Abcamab11355Mouse monoclonal [ICRF44] to CD11b50tests3996CD11bhystemcell一抗
Abcamab11356Mouse monoclonal [ICRF44] to CD11b （FITC）50tests3996CD11bhystemcell一抗
Abcamab11358Mouse monoclonal [A3D8] to CD4450tests3996CD44hystemcell一抗
Abcamab11359Mouse monoclonal [8.4A6] to ICAM1100ul3996CD54,ICAM1hystemcell一抗
Abcamab1136Rabbit polyclonal to Artemin100ul3996Arteminhystemcell一抗
Abcamab11364Mouse monoclonal [FMC46] to CD62L100ul3996CD62Lhystemcell一抗
Abcamab11365Mouse monoclonal [FMC-46] to CD62L （FITC）50tests3996CD62Lhystemcell一抗
Abcamab11366Mouse monoclonal [CXN-32] to Connexin 32/GJB150ul3996Connexin 32,GJB1hystemcell一抗
Abcamab11367Rabbit polyclonal to Connexin 32/GJB1100ul3996Connexin 32,GJB1hystemcell一抗
Abcamab11368Rabbit polyclonal to Connexin 32/GJB1100ul3996Connexin 32,GJB1hystemcell一抗
Abcamab11369Mouse monoclonal [CXN-6] to Connexin 43/GJA150ul3996Connexin 43,GJA1hystemcell一抗
Abcamab11370Rabbit polyclonal to Connexin 43/GJA1100ul4418Connexin 43,GJA1hystemcell一抗
Abcamab11371Mouse monoclonal [ZK-31] to Pan Desmocollin100ul3996Desmosomal Proteinhystemcell一抗
Abcamab11372Mouse monoclonal [P1H12] to CD14650ul3996CD146hystemcell一抗
Abcamab11375Rat monoclonal [YTH 8.18] to Macrophages+Neutrophils250ul3996Macrophage/Neutrophilshystemcell一抗
Abcamab11379Mouse monoclonal [8E9] to Glucocorticoid Receptor100ug3996Glucocorticoid Receptorhystemcell一抗
Abcamab1138Rabbit polyclonal to GRC5/PHF2100ul3996GRC5/PHF2hystemcell一抗
Abcamab11380Mouse monoclonal [MAB 263] to Growth hormone receptor100ug3996Growth hormone receptorhystemcell一抗
Abcamab11382Mouse monoclonal [INN-hCG-106] to hCG beta core fragment250ug3996hCG beta core fragmenthystemcell一抗
Abcamab11386Mouse monoclonal [INN-hFSH-158] to Glycoprotein hormones （alpha 5）250ug3996Glycoprotein hormones （alpha 5）hystemcell一抗
Abcamab11387Mouse monoclonal [INN-hCG-72] to hCG alpha 6250ug3996hCG alpha 6hystemcell一抗
Abcamab11388Mouse monoclonal [INN-hCG-2] to hCG beta 1 epitope250ug3996hCG beta 1hystemcell一抗
Abcamab11389Mouse monoclonal [INN-hCG-22] to hCG beta 2 epitope250ug3996hCG beta 2hystemcell一抗
Abcamab1139Rabbit polyclonal to HAP40100ul3996HAP40hystemcell一抗
Abcamab11390Mouse monoclonal [ INN-hCG-24 ] to hCG beta 4 epitope250ug3996hCG beta 4hystemcell一抗
Abcamab11391Mouse monoclonal [ INN-hCG-68 ] to hCG beta 7250ug3996hCG beta 7hystemcell一抗
Abcamab11392Mouse monoclonal [INN-hCG-53] to hCG Holo c2250ug3996hCG Holo c2hystemcell一抗
Abcamab11393Mouse monoclonal [INN-hCG-45] to hCG Holo c3250ug3996hCG Holo c3hystemcell一抗
Abcamab11396Mouse monoclonal [INN-hPL-37] to Human placental lactogen （epitope 2）250ug3996placental lactogenhystemcell一抗
Abcamab11399Rat monoclonal [ICR61] to EGFR100ug3996EGFRhystemcell一抗
Abcamab114Rabbit polyclonal to LIMPII100ul3996Scavenging Receptor SR-BIIhystemcell一抗
Abcamab1140Rabbit polyclonal to Cyclin E100ul3996Cyclin E1hystemcell一抗
Abcamab11400Rat monoclonal [ICR10] to EGFR （FITC）100ug5029EGFRhystemcell一抗
Abcamab11401Mouse monoclonal [F4] to EGFR （FITC）500ul3996EGFRhystemcell一抗
Abcamab11402Mouse monoclonal [H44] to IL18R1100ug3996IL-18R1hystemcell一抗
Abcamab11404Mouse monoclonal [H44] to IL18R1 （Biotin）50ug3996IL-18R1hystemcell一抗
Abcamab11408Mouse monoclonal [G16] to Interferon alpha125ug3996IFN-alphahystemcell一抗
Abcamab11409Sheep polyclonal to Interferon beta50ul3996IFN-betahystemcell一抗
Abcamab1141Rabbit polyclonal to Cyclin E2100ul3996Cyclin E2hystemcell一抗
Abcamab11411Mouse monoclonal [LMM775 ] to GCSF Receptor （Phycoerythrin）50tests3996G-CSF Receptorhystemcell一抗
Abcamab11412Mouse monoclonal [D9D10] to Interferon gamma250ug3996IFN-gammahystemcell一抗
Abcamab11413Mouse monoclonal [B27] to Interferon gamma （Allophycocyanin）50tests3996IFN-gammahystemcell一抗
Abcamab11414Mouse monoclonal [SN6] to CD105100ug3996CD105hystemcell一抗
Abcamab11415Mouse monoclonal [SN6] to CD105 （FITC）100ug5029CD105hystemcell一抗
Abcamab11417Mouse monoclonal [D9D10] to Interferon gamma （FITC）50ug3996IFN-gammahystemcell一抗
Abcamab11418Mouse monoclonal [Z-ZE4] to IGFBP150ug3996IGFBP1hystemcell一抗
Abcamab11419Mouse monoclonal [9C8] to GADD15350ug4418GADD153hystemcell一抗
Abcamab1142Rabbit polyclonal to Bcl10100ul3996Bcl-1hystemcell一抗
Abcamab11420Rabbit polyclonal to ERp2950ul3996ERp29hystemcell一抗
Abcamab11421Rabbit polyclonal to ERp5750ul3996ERp57hystemcell一抗
Abcamab11422Rabbit polyclonal to ERp7250ug3996ERp72hystemcell一抗
Abcamab11423Mouse monoclonal [1234] to Rapsyn50ug3996Rapsynhystemcell一抗
Abcamab11424Rabbit polyclonal to EDG150ug3996EDG1hystemcell一抗
Abcamab11425Mouse monoclonal [1351] to Syntrophin50ug3996Syntrophinhystemcell一抗
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