产品英文名称：PYROGENT™–5000 Kinetic Turbidimetric LAL Assays
产品中文名称：PYROGENT™–5000动态浊度法内毒素检测试剂盒
货号：N383
厂家：Lonza
品牌：Lonza
规格：96T/盒
产品应用：体外内毒素检测
保存温度： 2-8℃
货期：2～5天
有现货，促销中。

LONZA PYROGENT-5000 100 Test Kit 产品介绍：

PYROGENT -5000产品适合用于实验室处理大量样品，可以定量的动态检测革兰氏阴性细菌内毒素。检测在96孔板上进行，允许许多样品同时检测。The PYROGENT™–5000 assay是水样，大量注射用药物和医疗设备冲洗水内毒素检测的理想选择。检测使用温育板读数器340nm读数，检测灵敏度在0.01 to 100.0 EU/ml。

LONZA PYROGENT-5000 100 Test Kit 检测原理：

样品和LAL（鲎变形细胞裂物）反应物混合，革兰氏阴性细菌内毒素催化LAL酶原反应，反应初速度决 定于内毒素浓度。激活的凝固酶原水解LAL凝固蛋白酶原特定化学键形成凝固蛋白。水解形成的凝固蛋白会立即自我凝集成胶状物。LAL浊度法检测可以测定浊 度增加值（光密度），浊度变化在凝胶形成之前出现。反应物变混浊所需时间（反应时间）与细菌内毒素含量成反比例。也就是说，如果有大量内毒素存在，则反应 迅速，很快就会出现混浊；如果有少量内毒素存在反应时间就会延长。未知样品内毒素浓度可通过标准曲线计算出。

LONZA PYROGENT-5000 100 Test Kit 注意事项：

¡ 本品为体外诊断试剂，严禁用于人体内毒素检测
¡ 本品用于药品的G-细菌内毒素检测时，应遵循USP药典细菌内毒素检测法的规定
¡ 如果说明书下载不了或者有其它产品信息问题可以咨询

LONZA PYROGENT-5000 100 Test Kit 英文介绍：

The PYROGENT™–5000 assay is perfect for laboratories needing to process large numbers of samples. This assay is performed in a 96-well plate allowing many samples to be processed at one time. It is ideal for water samples, large volume parenterals and water rinse from medical devices. The PYROGENT™–5000 assay is measured at the 340 nm wavelength and has a sensitivity range from 0.01 to 100.0 EU/ml. An incubating plate reader is required to run this assay.

PYROGENT -5000 is a quantitative, kinetic assay for the detection of Gram negative bacterial endotoxin. A sample is mixed with the reconstituted LAL reagent, placed in the photometer, and automatically monitored over time for the appearance of turbidity. The time required before the appearance of turbidity （Reaction Time） is inversely proportional to the amount of endotoxin present. That is, in the presence of a large amount of endotoxin the reaction occurs rapidly; in the presence of a smaller amount of endotoxin the reaction time is increased. The concentration of endotoxin in unknown samples can be calculated from a standard curve.