Fluorescent Probes（荧光探针）—cayman品牌推荐
Cayman拥有500多种荧光探针，帮助您提升研究中所需的多功能性、灵敏度和定量能力。这一系列探针能够检测细胞内活动、蛋白质相互作用、一系列酶活性和许多其他重要目标蛋白。为实验选择最合适的荧光染料请参考以下cayman荧光探针表格。
检测细胞活力和细胞增殖的探针
| Item No. | Product Name | Detects | Cell Permeable? |
Excitation Maximum (nm) |
Emission Maximum (nm) |
| 11397 | 7-Aminoactinomycin D | DNA; non-viable cells | × | 488,546,578 | 650 |
| 14948 | Calcein AM* | cell viability | √ | 494 | 517 |
| 20637 | Calcein Blue AM* | cell viability | √ | 360 | 445 |
| 20632 | Calcein Red™ AM* | cell viability | √ | 560 | 574 |
| 20639 | Calcein UltraBlue™ AM* | cell viability (higher photostability and stronger fluorescence intensity atphysiological pH than Calcein Blue) | √ | 360 | 445 |
| 14456 | CFDA-SE | cell proliferation | √ | 491 | 518 |
| 20698 | CytoTrace™ Red CMTPX | cell division; cell tracking | √ | 577 | 602 |
| 35701 | Fluorescein diacetate | cell viability | √ | 490 | 520 |
| 19583 | Green CMFDA | cell division; cell tracking | √ | 492 | 517 |
| 15003 | JC-1 | mitochondrial integrity; apoptosis | √ | 485(monomer) 520-570(aggregate) |
535(monomer) 570-610(aggregate) |
| 14289 | Propidium Iodide | DNA; RNA; non-viable cells | × | 488-535 | 617 |
| 14322 | Resazurin (sodium salt) | enzyme activity; cell viability | √ | 570 | 580 |
| 21426 | Tetramethylrhodamine ethyl ester (perchlorate) | mitochondrial function; cell viability | √ | 550 | 575 |
*Also available: membrane-impermeable non-AM forms
View a complete list of cell viability fluorescent probes at www.caymanchem.com
检测核苷酸的探针
| Item No. | Product Name | Detects | Cell Permeable? |
Excitation Maximum (nm) |
Emission Maximum (nm) |
| 14338 | Acridine Orange | Nucleic acids; pH | √ | 502(dsDNA) 460(ssDNA andRNA) 475 (low pH) |
525(dsDNA) 650(ssDNA and RNA) 590(low pH) |
| 22439 | 9-Amino-6-chloro-2- methoxyacridine | DNA and pH gradients; poly(dA-dT) sequences of DNA | √ | 411 | 475 |
| 11315 | Chromomycin A3 | DNA (two or more contiguous GC base pairs) | × | 445 | 575 |
| 14285 | DAPI (hydrochloride) | DNA (AT-rich sequences) | × | 358 | 461 |
| 15547 | Hoechst 33342 (hydrochloride) | DNA (AT-rich sequences); nuclear marker | √ | 350 | 461 |
| 14488 | Pyronin Y | dsRNA | √ | 540-550 | 560-580 |
View a complete list of DNA fluorescent probes at www.caymanchem.com
检测氧化应激和活性反应组分的探针
| Item No. | Product Name | Detects |
Excitation Maximum (nm) |
Emission Maximum (nm) |
| 10157 | APF | hydroxyl radical; hypochlorite ion; certain peroxidase intermediates | 490 | 515 |
| 27086 | C11 BODIPY 581/591 | lipid oxidation | 581/500(oxidized) | 591/510(oxidized) |
| 14051 | Coumarin Boronic Acid | peroxynitrite; hypochlorous acid; hydrogen peroxide | 332 | 470 |
| 19111 | Coumarin hydrazine | reactive carbonyl groups | 365 | 430/550 (upon reaction with carbonyls) |
| 85165 | DAF-2 diacetate | nitric oxide | 485 | 538 |
| 18767 | DAF-FM diacetate | nitric oxide | 495 | 515 |
| 85155 | 2,7-Dichlorodihydrofluorescein diacetate | peroxynitrite formation | 502 | 523 |
| 12013 | Dihydroethidium | superoxide | 490 | 590 |
| 85100 | Dihydrorhodamine 123 | peroxynitrite formation | 500 | 536 |
| 62237 | DPPP | hydroperoxides; low-density lipoprotein; cellular oxidation | 351 | 380 |
| 34564 | 7-Fluoro-2,1,3-benzoxadiazole- 4-sulfonate (ammonium salt) | thiols | 380 | 515 |
| 14872 | Lucigenin | superoxide; chloride | 455 | 505 |
| 16803 | Luminol | peroxidation; iron; gold; cyanide | 355 | 411 |
| 17097 | Monobromobimane | reactive sulfur; thiol groups | 398 | 490 |
| 10005983 | Pentafluorobenzenesulfonyl fluorescein | hydrogen peroxide | 465-505 | 505-555 |
| 16929 | WSP-5 | hydrogen sulfide | 502 | 525 |
View a complete list of fluorescent probes for oxidative stress and reactive species detection at www.caymanchem.com
检测细胞骨架蛋白的探针
| Item No. | Product Name | Detects |
Excitation Maximum (nm) |
Emission Maximum (nm) |
| 21016 | 9-(2,2-Dicyanovinyl)julolidine | tubulin and other cytoskeletal proteins | 450 | 480(low viscosity solvents) 505(high viscosity solvents) |
| 20546 | Phalloidin-California Red™ Conjugate | F-actin | 583 | 605 |
| 20478 | Phalloidin-Fluorescein Conjugate | F-actin | 492 | 518 |
| 20547 | Phalloidin-iFluor™ 350 Conjugate | F-actin | 353 | 442 |
| 20548 | Phalloidin-iFluor™ 405 Conjugate | F-actin | 400 | 421 |
| 20549 | Phalloidin-iFluor™ 488 Conjugate | F-actin | 493 | 517 |
| 20552 | Phalloidin-iFluor™ 555 Conjugate | F-actin | 556 | 574 |
| 20553 | Phalloidin-iFluor™ 594 Conjugate | F-actin | 590 | 618 |
| 20554 | Phalloidin-iFluor™ 633 Conjugate | F-actin | 634 | 649 |
| 20555 | Phalloidin-iFluor™ 647 Conjugate | F-actin | 650 | 665 |
| 20545 | Phalloidin-Tetramethylrhodamine Conjugate | F-actin | 546 | 575 |
View a complete list of cytoskeletal protein fluorescent probes at www.caymanchem.com
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| Actin filaments stained with Phalloidin-iFluor™ 350 Conjugate |
HeLa cells stained with Phalloidin-iFluor™ 488 Conjugate |
HeLa cells labeled with Phalloidin-iFluor™ 594 Conjugate |
蛋白酶底物探针
| Item No. | Product Name | Detects |
Excitation Maximum (nm) |
Emission Maximum (nm) |
| 9002021 | Abz-Val-Ala-Asp-Nva-Arg-Asp- Arg-Gln-EDDnp (trifluoroacetate salt) | human proteinase 3 activity | 320 | 420 |
| 26640 | Ac-ANW-AMC | β5i/LMP7 subunit of the 20S immunoproteasome activity | 340-360 | 440-460 |
| 14986 | Ac-DEVD-AMC | caspase-3 activity | 340-360 | 440-460 |
| 17051 | Ac-LEHD-AFC (trifluoroacetate salt) | caspase-4, -5, and -9 activity | 400 | 505 |
| 14991 | Ac-LETD-AFC | caspase-8 activity | 400 | 505 |
| 14988 | Ac-VDVAD-AFC | caspase-2 activity | 400 | 505 |
| 14992 | Ac-WEHD-AFC (trifluoroacetate salt) | caspase-1, -4, and -5 activity | 400 | 505 |
| 17591 | Ac-YVAD-AFC | caspase-1 and -4 activity | 400 | 505 |
| 27580 | Gly-Arg-AMC (hydrochloride) | cathepsin C activity | 340-360 | 440-460 |
| 14907 | MeOSuc-Ala-Ala-Pro-Val-AMC | human leukocyte and porcine pancreatic elastase activity | 355-380 | 440-460 |
| 10008119 | Suc-Leu-Leu-Val-Tyr-AMC | 20S proteasome, chymotrypsin-like proteases, and calpain activity | 360 | 460 |
| 11675 | (Z-Ala-Ala-Ala-Ala)2Rh110 | elastase activity | 485 | 525 |
| 10008117 | Z-LLE-AMC | caspase-like activity of the 26S proteasome or 20S proteolytic core | 340-360 | 440-460 |
View a complete list of protease activity fluorescent probes at www.caymanchem.com
其他酶底物探针
| Item No. | Product Name | Detects |
Excitation Maximum (nm) |
Emission Maximum (nm) |
| 62910 | 7-hydroxycoumarinyl Arachidonate | cPLA2 activity | 335 | 450 |
| 25583 | C12FDG | β-galactosidase activity | 485 | 530 |
| 35500 | DiFMUP | phosphatase activity | 358 | 455 |
| 16551 | 4-Methylumbelliferyl-α-D- Galactopyranoside | α-galactosidase activity |
330(pH 4.6); 370 (pH 7.4); 385(pH 10.4) |
445-454 |
| 20948 | 4-Methylumbelliferyl-β-D- Glucopyranoside | β-glucosidase and β-glucocerebrosidase activity |
330(pH 4.6); 370 (pH 7.4); 385(pH 10.4) |
445-454 |
| 19543 | 4-Methylumbelliferyl-α-L- Iduronide (free acid) | α-L-iduronidase activity | 320(pH 1.97-6.72); 360(pH 7.12-10.3) |
445-455 (increases as pH decreases) |
| 19524 | 4-Methylumbelliferyl 6-thio- Palmitate-β-D-Glucopyranoside | palmitoyl-protein thioesterase (PPT/CLN1) activity | 320(pH 1.97-6.72); 360(pH 7.12-10.3) |
445-455 (increases as pH decreases) |
View a complete list of enzyme substrate fluorescent probes at www.caymanchem.com
检测蛋白交互作用探针
| Item No. | Product Name | Detects |
Excitation Maximum (nm) |
Emission Maximum (nm) |
| 20672 | 3-BODIPY-propanoylaminocaproic Acid N-hydroxysuccinimide ester | proteins immobilized on PVDF membranes | 300-360,504 | 511 |
| 20704 | FlAsH-EDT2 | proteins with a tetracysteine sequence | 508 | 528 |
| 19767 | ReAsH-EDT2 | proteins with a tetracysteine sequence | 593 | 608 |
View a complete list of probes to detect protein interactions at www.caymanchem.com
检测钙离子探针
| Item No. | Product Name | Detects |
Cell Permeable? |
Excitation Maximum (nm) |
Emission Maximum (nm) |
| 20424 | BTC AM* | intracellular calcium (low affinity) | √ | 401(Ca2+-bound)/480 | 529 |
| 35758 | Fluo-2 AM* | intracellular calcium | √ | 490 | 515 |
| 14960 | Fluo-3 AM* | intracellular calcium | √ | 488 | 526 |
| 31143 | Fluo-4 AM* | intracellular calcium | √ | 490 | 520 |
| 14591 | Fura-2 AM* | intracellular calcium | √ | 380 (low [Ca2+]) 340 (high [Ca2+]) |
510 |
| 34993 | Fura-2 Leakage Resistant AM* | intracellular calcium | √ | 340 (Ca2+-bound) 380 (Ca2+-free) |
505 |
| 20416 | Fura-FF AM* | intracellular calcium (low affinity) | √ | 365 (no Ca2+) 339 (high [Ca2+]) |
514(no Ca2+) 507(high [Ca2+]) |
| 34985 | ICR-1 AM* | intracellular calcium | √ | 580 | 660 |
| 20417 | Indo-1 AM* | intracellular calcium | √ | 349-364 | 475-485(no Ca2+) 400-410(Ca2+-bound) |
| 20442 | Rhod-5N AM* | intracellular calcium (low affinity) | √ | 551 | 576 |
| 20444 | Rhod-FF AM* | intracellular calcium (low affinity) | √ | 552 | 580 |
*Also available: membrane-impermeable non-AM forms
View a complete list of calcium fluorescent probes at www.caymanchem.com
检测pH值探针
| Item No. | Product Name | Detects | Cell Permeable? |
Excitation Maximum (nm) |
Emission Maximum (nm) |
| 17183 | BCECF | pH | × | 440 / 490 | 535 |
| 15922 | BCECF AM | intracellular pH | √ | 440 / 490 | 535 |
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