Lightning Link 系列金属标记试剂盒

Lightning Link 系列金属标记试剂盒

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Expedeon 2019 年新产品 Lightning Link 系列金属标记试剂盒现在有多达30种啦!

  • 手工操作只需30秒!

  • 1 小时内完成标记

  • 无繁琐的分离步骤,100% 抗体回收率

  • 温和化学反应,标记物稳定

  • 可选金属多,以便多路测定

  • 批间重复性好

  • 应用广泛

操作步骤

Lightning Link 系列金属标记试剂盒

可选标记

Lightning-Link® 金属标记试剂盒
Lightning-Link®  139La Lightning-Link® 159Tb
Lightning-Link®  141Pr Lightning-Link® 160Gd
Lightning  Link®-142Nd Lightning-Link® 162Dy
Lightning-Link®  143Nd Lightning-Link® 164Dy
Lightning-Link®  144Nd Lightning-Link® 165Ho
Lightning-Link®  146Nd Lightning Link®-166Er
Lightning-Link®  147Sm Lightning Link®-167Er
Lightning-Link®  148Nd Lightning-Link® 168Er
Lightning-Link®  149Sm Lightning-Link® 169Tm
Lightning-Link®  151Eu Lightning-Link® 170Er
Lightning-Link®  152Sm Lightning-Link® 171Yb
Lightning-Link®  153Eu Lightning-Link® 172Yb
Lightning-Link®  154Sm Lightning-Link® 174Yb
Lightning  Link®-156Gd Lightning-Link® 175Lu
Lightning-Link®  158Gd Lightning-Link® 176Yb


实验数据

Lightning Link 系列金属标记试剂盒

Figure 1. Goat Anti-Rabbit was labeled with Lightning-Link® 159Tb at 100 µg single reaction vial and tested with Rabbit IgG in a sandwich ELISA. Detection: Dissociation-enhanced lanthanide fluorescence.

Lightning Link 系列金属标记试剂盒

Figure 2. Goat Anti-Rabbit was labeled with a selection of Lightning-Link® metal labels at 10 µg single reaction vials. 2 μg conjugates per well were run on a 4-12% Bis-Tris gel (Expedeon, NBT41212), under non-reducing conditions for 20 minutes at 50V and 1 hour at 120V in 1x MOPS (Expedeon, NXB75500). The gel was stained with InstantBlue™ (Expedeon, ISB1L) for 1 hour on the gel rocker. The gel showed no free antibody post conjugation for all conjugates except when the reaction was quenched at t=0, where no conjugation was observed.

Figure 3. X-Ray Fluorescence Lα series peaks used to quantify the metal content in Lightning-Link® Metal Labeling kit.

应用

  • X-ray fluorescence

  • Mass cytometry

  • Inductively Coupled Plasma Mass Spectrometry (ICP-MS)

  • Dissociation-enhanced fluorescence immunoassays (**DELFIA®)

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