Lightning Link 系列金属标记试剂盒

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Expedeon 2019 年新产品 Lightning Link 系列金属标记试剂盒现在有多达30种啦！

• 手工操作只需30秒！

• 1 小时内完成标记

• 无繁琐的分离步骤，100% 抗体回收率

• 温和化学反应，标记物稳定

• 可选金属多，以便多路测定

• 批间重复性好

• 应用广泛

操作步骤

可选标记

实验数据

Figure 1. Goat Anti-Rabbit was labeled with Lightning-Link^® 159Tb at 100 µg single reaction vial and tested with Rabbit IgG in a sandwich ELISA. Detection: Dissociation-enhanced lanthanide fluorescence.

Figure 2. Goat Anti-Rabbit was labeled with a selection of Lightning-Link^® metal labels at 10 µg single reaction vials. 2 μg conjugates per well were run on a 4-12% Bis-Tris gel (Expedeon, NBT41212), under non-reducing conditions for 20 minutes at 50V and 1 hour at 120V in 1x MOPS (Expedeon, NXB75500). The gel was stained with InstantBlue™ (Expedeon, ISB1L) for 1 hour on the gel rocker. The gel showed no free antibody post conjugation for all conjugates except when the reaction was quenched at t=0, where no conjugation was observed.

Figure 3. X-Ray Fluorescence Lα series peaks used to quantify the metal content in Lightning-Link^® Metal Labeling kit.

应用

• X-ray fluorescence

• Mass cytometry

• Inductively Coupled Plasma Mass Spectrometry (ICP-MS)

• Dissociation-enhanced fluorescence immunoassays (**DELFIA^®)

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