VectaPlex常见问题解答

VectaPlex™ Antibody Removal Kit (VRK-1000)

Q: VectaPlex™可用于冰冻切片吗?

A: No. During development, VectaPlex™ was optimized for FFPE tissues, where the power to remove antibodies at room temperature was balanced with the need to preserve tissue morphology. For frozen tissues, VectaPlex™ will remove the tissue from the slide.

Q: VectaPlex™ 可以去除免疫组化 (IHC) 实验应用中的抗体染色（酶显色）吗？

A: VectaPlex™ Antibody Removal Kit is designed to be used in fluorescent workflows such as immunofluorescence or TSA. It has not been validated or confirmed for immunohistochemistry (IHC) applications. VectaPlex™ may remove substrates already deposited on tissue samples.

Q: 我有使用TrueView试剂盒去除组织的自发荧光, 请问TrueVIEW会被VectaPlex™试剂去除吗?

A: We used TrueView in our VectaPlex™ IF workflow to suppress autofluorescence. Application of VectaPlex™ removes some, but not all, of the TrueView reagent. Since some TrueView remained after VectaPlex™ application, we did not add TrueView in every cycle, but only as needed to suppress autofluorescence.

Q: 请问将VectaPlex™试剂盒放置到4℃冰箱一段时间，还可以使用吗?

A: Yes. VectaPlex™can withstand a freeze-thaw cycle and still perform as expected, but the recommended storage is at ambient temperature (15-25 °C). Additionally, the kit will still perform properly if placed at 4 °C.

Q: 在同一张切片上使用 VectaPlex™,是否有最大次数限制呢？

A: VectaPlex™ has been tested for up to 6 cycles with no ill effects. We don’t expect there to be issues with increasing the number of cycles, but we suggest testing with controls for adequate comparisons. We recommend running experiments to confirm that the use of more cycles does not impact the antigenicity or tissue morphology.

Q: VectaPlex™可用于自动化染色平台吗?

A: We have not tested this product on automated systems and cannot guarantee instrument compatibility at this time.

Q: VectaPlex™ 是否适用于所有种属来源的抗体?

A: VectaPlex™ has been tested with mouse and rabbit primaries, and with horse and goat secondaries. We expect it to work well with other species of antibodies as well. 

Q: VectaPlex™ 是否适用于不同类型的组织样本?

A: VectaPlex™ has been tested on and works well with various FFPE tissues, including FFPE breast and lung tissue which are very fragile.

Q: VectaPlex™ 是否与所有抗体相兼容?

A: VectaPlex™ has been tested on more than 30 common antibodies and has worked on them without issue.

Q: 在IF实验中, VectaPlex™ 抗体去除试剂是否也可以去除组织的自发荧光呢？

A: VectaPlex™ does not appear to reduce autofluorescence background.

Q: 是否可以提供使用VectaPlex™操作过程中移去盖玻片的操作流程呢?

A: We recommend using VectaShield® Plus Antifade Mounting Media with DAPI (H-2000), which is an aqueous non-hardening mounting medium. After each imaging cycle, the coverslip can be easily removed by incubating in PBS and the residual VectaShield® removed with a PBS wash.