15-deoxy-Δ12,14-PGJ2 ELISA kit——ENZO热销产品

首款上市的15-d-PGJ2 ELISA试剂盒，可用于类花生酸研究

 

15-deoxy-Δ12,14-PGJ2（15-d-PGJ2）是PGD2的最终脱水产物之一，通过中间体Δ12-PGJ2形成。生理条件下，15-d-PGJ2存在于体液中，浓度介于10^(-12)至10^(-9)M，但在感染和炎症等应激条件下会急剧增加。在细胞类型和浓度的影响下，15-d-PGJ发挥促进和抗炎症作用，其中许多作用是通过与过氧化物酶体增殖剂激活受体（PPAR-γ）的γ异构体结合而介导的。15-d-PGJ2似乎通过一种与PPAR-γ无关的机制诱导HO-1的表达，该机制由活性氧（ROS）的产生介导。

Enzo Life Sciences的15-脱氧-Δ12,14-前列腺素J2 ELISA试剂盒是一种比色竞争法酶联免疫检测试剂盒，可在5小时内得到检测结果，相比GC/MS 分析节省时间且耗时少得多。上海金畔生物作为Enzo Life Sciences中国代理，可提供该产品，欢迎咨询。

 

产品特色

◆ 灵敏度高，可测量低至36.8 pg/ml的15-deoxy-Δ12,14-PGJ2

◆ 检测快速，较GC/MS分析耗时少得多

◆ 易于使用的即用型液体颜色编码试剂，可减少错误

◆ 优于半定量Western blot分析的完全定量检测

  

产品信息

产品货号	ADI-900-023/ ADI-901-023
产品名称	15-deoxy-Δ12,14-PGJ2 ELISA kit(15-脱氧-Δ12,14-前列腺素J2 ELISA试剂盒)
规格	96 wells/ 5×96 wells
其他名称	15-deoxy-Δ12,14-Prostaglandin J2
灵敏度	36.8 pg/ml
检测范围	195 – 200,000 pg/ml
检测时间	5 hours
应用	ELISA, Colorimetric detection
检测波长	405 nm
样本类型	细胞培养上清，血浆，唾液和尿液
适用种属	不限种属
试剂盒组分	GxR IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer concentrate,  Standard, pNpp Substrate, Stop solution

 

标曲示例

 

 

部分产品引用文献

1. Redox aspects of cytotoxicity and anti-neuroinflammatory profile of chloroquine and hydroxychloroquine in serum-starved BV-2 microglia: L. Račková, et al.; Toxicol. Appl. Pharmacol. 447, 116084 (2022)

2. Fluoxetine‐induced hepatic lipid accumulation is mediated by prostaglandin endoperoxide synthase 1 and is linked to elevated 15‐deoxy‐Δ12, 14PGJ2: A. Ayyash, et al.; J. Appl. Toxicol. 4272, 1002 (2021)

3. Oxylipin biosynthesis reinforces cellular senescence and allows detection of senolysis: C.D. Wiley, et al.; Cell Metab. 33, 1124 (2021)

4. Cocaine-mediated circadian reprogramming in the striatum through dopamine D2R and PPARγ activation: K. Brami-Cherrier, et al.; Nat. Commun. 11, 4448 (2020)

5. Thymopentin improves the survival of septic mice by promoting the production of 15-deoxy-prostaglandin J2 and activating the PPARγ signaling pathway: Y. Zhang, et al.; FASEB J. 34, 11772 (2020)

6. Apolipoprotein D overexpression alters hepatic prostaglandin and omega fatty acid metabolism during the development of a non-inflammatory hepatic steatosis: F. Desmarais, et al.; Biochim. Biophys. Acta Mol. Cell Biol. Lipids 1864, 522 (2019)

7. Association of chronic inflammation and perceived stress with abnormal functional connectivity in brain areas involved with interoception in hepatitis C patients: G. Oriolo, et al.; Brain Behav. Immun. 80, 204 (2019)

8. Programming of macrophages by UV-irradiated apoptotic cancer cells inhibits cancer progression and lung metastasis: Y.B. Kim, et al.; Cell. Mol. Immunol. 16, 851 (2019)

9. Arachidonic acid induces ARE/Nrf2-dependent heme oxygenase-1 transcription in rat brain astrocytes: C.C. Lin, et al.; Mol. Neurobiol. 55, 3328 (2018)

10. Bone-marrow-derived mesenchymal stem cells inhibit gastric aspiration lung injury and inflammation in rats: J. Zhou, et al.; J. Cell Mol. Med. 20, 1706 (2016)

11. Implications of a genetically determined nitric oxide deficit for endothelial cell-leukocyte interaction and cardiovascular disease: I. Kadiyska; (2016)

12. CRTH2 is a critical regulator of neutrophil migration and resistance to polymicrobial sepsis: M. Ishii, et al.; J. Immunol. 188, 5655 (2012)

13. Choroidal involution is a key component of oxygen-induced retinopathy: Z. Shao, et al.; Invest. Ophthalmol. Vis. Sci. 52, 6238 (2011)

14. Response of chondrocytes to shear stress: antagonistic effects of the binding partners Toll-like receptor 4 and caveolin-1: P. Wang, et al.; FASEB J. 25, 3401 (2011)

15. The myocardial infarct size-limiting effect of sitagliptin is PKA-dependent, whereas the protective effect of pioglitazone is partially dependent on PKA: Y. Ye, et al.; Am. J. Physiol. Heart Circ. Physiol. 298, H1454 (2010)

 

 

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