ENZO热销产品——VEGF (human), ELISA kit

血管内皮生长因子(VEGF)最初被称为血管通透性因子(VPF)，是一个刺激血管发生和血管新生的蛋白质家族。VEGF家族包括五个成员：VEGF-A、VEGF-B、VEGF-C、VEGF-D和PGF（胎盘生长因子）。作为第一个被发现的VEGF家族成员，VEGF-A常被简称为VEGF。VEGF-A基因由8个外显子组成，选择性剪接产生10种以上亚型，包括VEGF 121、VEGF 145、VEGF 165、VEGF 183、VEGF 189和VEGF 206等。VEGF165和VEGF121是在大多数细胞和组织中表达的主要亚型。VEGF165是一种45KDa同源二聚体糖蛋白，对肝素具有中等亲和力。相反，VEGF121不与肝素结合。VEGF特异性结合酪氨酸激酶受体VEGFR-1(Flt-1)和VEGFR-2(Flk-1)，激活下游信号通路，导致内皮细胞存活、增殖、迁移和血管通透性。VEGF通过增强血管通透性或招募内皮祖细胞到新生血管形成部位远处位点，在病理性血管生成中起着核心作用。随着对VEGF促进病理性血管生成和肿瘤转移的深入了解，在多种临床模型中，阻断VEGF/VEGFR轴抑制血管生成和肿瘤生长已成为合理的研究方向。

ENZO VEGF ELISA kit是一种灵敏的比色酶免疫测定试剂盒，可在2.5小时内得到检测结果。在450 nm处读取吸光度。

● 高灵敏度，可检测低至4.712 pg/ml的VEGF

● 高特异性，与VEGF 165的交叉反应为100%，与包括VEGF 121在内的相关分子几乎没有交叉反应

● 高通量，可检测多达38个样本（带复孔），仅需2.5h即可获得结果

● 彩色编码试剂有助于减少错误并提高效率

产品信息

部分产品文献引用

1. Negative Modulation of the Angiogenic Cascade Induced by Allosteric Kinesin Eg5 Inhibitors in a Gastric Adenocarcinoma In Vitro Model: A. Ricci, et al.; Molecules 27, 957 (2022) 

2. Attenuation of high glucose-induced damage in RPE cells through p38 MAPK signaling pathway inhibition: G. Maugeri, et al.; Front. Pharmacol. 12, 684680 (2021), Application(s): ELISA using culture supernatants 

3. Mifepristone as a potential therapy to reduce angiogenesis and p-glycoprotein associated with glioblastoma resistance to temozolomide: M. Llaguno-Munive, et al.; Front. Oncol. 10, 581814 (2020) 

4. The combination of sulforaphane and Fernblock® XP improves individual beneficial effects in normal and neoplastic human skin cell lines: S. Serini, et al.; Nutrients 12, 1608 (2020), Application(s): ELISA using culture supernatants

5. Aflibercept regulates retinal inflammation elicited by high glucose via the PlGF/ERK pathway: F. Lazzara, et al.; Biochem. Pharmacol. 168, 341 (2019), Application(s): ELISA using culture supernatants

6. Mechanism of the anti-angiogenic effect of avemar on tumor cells: N.G. Imir, et al.; Oncol. Lett. 15, 2673 (2018), Application(s): ELISA using culture supernatants 

7. The Role of Human Umbilical Vein Endothelial Cells in Osteogenic Differentiation of Dental Follicle-Derived Stem Cells in In Vitro Co-cultures: J.S. Bok, et al.; Int. J. Med. Sci. 15, 1160 (2018)

8. The effect of etanercept on vascular endothelial growth factor production by cutaneous mesenchymal stem cells from patients with psoriasis: A. Campanati, et al.; J. Int. Med. Res. 44, 6 (2016), Application(s): VEGF quantification, human MSC cells 

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