Rabbit anti-ZNF300 Polyclonal Antibody别名宿主特异性反应种属预测反应种属应用分子量免疫原浓度纯化方法类型同种型储存/保存方法存储溶液研究领域背景说明细胞定位UniProt
| 概述 | |
| 别名 |
ZNF300抗体;Kruppel like zinc finger protein; Zinc finger protein 300; ZN300_HUMAN
|
| 宿主 |
Rabbit
|
| 特异性 |
ZNF300
|
| 反应种属 |
Mouse
|
| 预测反应种属 |
Human;
|
| 应用 |
WB=1:500-2000 ELISA=1:500-1000
|
| 分子量 |
Predicted molecular weight: 68kDa
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 免疫原 |
KLH conjugated synthetic peptide derived from human ZNF300:165-250/604
|
| 性能 | |
| 浓度 |
1mg/ml
|
| 纯化方法 |
affinity purified by Protein A
|
| 类型 |
Polyclonal Antibody
|
| 同种型 |
IgG
|
| 储存/保存方法 |
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
|
| 存储溶液 |
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
|
| 研究领域 |
染色质和核信号 信号转导 转录调节因子
|
| 靶标 | |
| 背景说明 |
ZNF300 contains C2H2 (or Kruppel)-type zinc finger motifs that tetrahedrally coordinate zinc ions and form a protein structure that can bind DNA and regulate transcription (Gou et al.,2004 [PubMed 14746915]). ZNF300 belongs to the krueppel C2H2 type zinc finger protein family. It contains twelve C2H2 type zinc fingers and one KRAB domain. ZNF300 has a transcriptional repressor activity. Expressed mostly in heart, skeletal muscle and brain. Isoforms 1 and 2 are highly expressed in testis. There are three named isoforms.
|
| 细胞定位 |
细胞核
|
| UniProt |
Q96RE9
|
实验结果图
Sample:
Lane1: Testis (Mouse) Lysate at 30 ug
Lane2: Small intestine (Mouse) Lysate at 30 ug
Primary: Anti-ZNF300(JP120084a) at 1:200 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1:3000 dilution;
Predicted band size : 66kD
Observed band size : 70,66kD