Streck 230253 Hybrid Plastic Cell-Free DNA BCT 第二代升级新型混合塑料采血管中所含的保存剂能稳定有核血细胞，防止释放细胞基因组DNA，抑制核酸酶介导的无细胞DNA的降解，有助于无细胞DNA的整体稳定。
Fusion2是一种特制的混合塑料，兼具玻璃的Z佳优点和塑料的安全性。

Streck 230253 9mL Hybrid Plastic Cell-Free DNA BCT 第二代升级新型混合塑料采血管

 

Streck 230253 Hybrid Plastic Cell-Free DNA BCT 第二代升级新型混合塑料采血管，Streck Cell-Free DNA BCT Blood Collection Tube 218976, 218962基因无创管，唐氏无创筛查采血管，游离DNA采血管，游离RNA采血管，循环肿瘤细胞（circulating tumor cell）CTC采血管  现货供应

 

 ，  -：

 

Streck 230253 9mL Hybrid Plastic Cell-Free DNA BCT 第二代升级新型混合塑料采血管

   

Streck 230253 9mL Hybrid Plastic Cell-Free DNA BCT 第二代升级新型混合塑料采血管内含细胞保存剂，能稳定有核血细胞，防止胞内基因组DNA的释放，保存血浆中游离DNA和循环肿瘤细胞（CTC），提供了用于临床研究、药物开发和诊断检测的无细胞血浆DNA和CTC的样本采集、运送和保存的标准化方法。

 

出于安全考虑我们采用将玻璃和塑料的zui佳性能融合在一起的Fusion2^TM新型混合塑料管。具有700倍以下的WVTR，有效减少水分流失，保护试剂和样品完整性。具有10倍的低气渗透率，保证真空稳定性，延长Streck Cell-Free DNA BCT®全血标本保鲜管保质期。

产品订购信息

品牌	货号	产品描述
Streck	230252	6-Tube Pack (9 mL) Hybrid Plastic, RUO
Streck	230253	100-Tube Box (9 mL) Hybrid Plastic, RUO
Streck	230254	1000-Tube Box (9 mL) Hybrid Plastic, RUO
Streck	230255	6-Tube Pack (9 mL) Hybrid Plastic, CE
Streck	230256	100-Tube Box (9 mL) Hybrid Plastic, CE
Streck	230257	1000-Tube Box (9 mL) Hybrid Plastic, CE

RUO：仅供科研使用

 

【STRECK无创管的产品原理】

STRECK Cell-Free DNA BCT Blood Collection Tube应用于血液中的核酸检测，包括血浆中的无细胞DNA检测和分析，在疾病诊断中是一个新兴和可靠的领域。虽然健康人体的血浆中只有少量无细胞DNA，但血液循环中的无细胞DNA水平的增减与许多临床疾病有关。研究表明，STRECK无创采血管作为一种非侵入性的，快速灵敏的诊断工具，可应用于急性病的分子诊断和检测，以及胎儿遗传疾病的产前诊断。

      ，  -：

Streck 230253 9mL Hybrid Plastic Cell-Free DNA BCT Fusion2 第二代升级新型混合塑料采血管中所含的保存剂能稳定有核血细胞，防止释放细胞基因组DNA，抑制核酸酶介导的无细胞DNA的降解，有助于无细胞DNA的整体稳定。

STRECK无创管在保护细胞表面抗原性，维持细胞形态的同时，加强无细胞DNA的提取、检测和分析。采集在STRECK无创管中的样本，可在6-37℃下稳定保存14天，方便样品的采集、运输和储藏。STRECK无创管可用于临床研究、药品发现和加强诊断检测。

 

添加剂

STRECK无创管含有K3EDTA抗凝剂和一种液态的细胞保存剂。

 

材质

Fusion2是一种特制的混合塑料，兼具玻璃的zui佳优点和塑料的安全性。

 

STRECK无创管保存和稳定性

1.2-30℃下，空管效期内都能使用。

2.2-40℃下，空管能保存14天。

3.空管不能冷冻。极端温度条件下运送时，需要采取适当的隔离措施。

4.样本保存/稳定性

样本类型	游离DNA	胞内基因组DNA	肿瘤细胞（上皮细胞）
样本保存时间	14天	14天	7天
样本保存温度	6-37℃	6-37℃	15-30℃

 

【STRECK无创管特点】

• 省时省力

STRECK无创管提供了一种处理病人血液样本的检测和分析无细胞DNA的方案，比传统的血浆样本制备方法更节省人力和时间。

• 方便统筹

STRECK无创管中的样本可在室温下保存长达14天，样本采集、运输和储存更加方便。

• 用途广泛

STRECK无创管获得的高品质无细胞DNA可用于各种临床医学研究、药品发现和诊断检测。

• 结果准确

Streck 230253 9mL Hybrid Plastic Cell-Free DNA BCT Fusion2 第二代升级新型混合塑料采血管只需对样本进行极少的处理，增强样本的稳定性和无细胞血浆DNA的提取，将无细胞DNA样本制备的变异风险降至zui低。

【STRECK无创管使用说明】

Streck 230253 9mL Hybrid Plastic Cell-Free DNA BCT Fusion2 第二代升级新型混合塑料采血管操作程序

1、根据CLSI GP41-A61采集血液进入试管。

——防止回流

由于Streck 230253 9mL Hybrid Plastic Cell-Free DNA BCT Fusion2 第二代升级新型混合塑料采血管基因检测无创采血管含有化学添加剂，应尽量防止液体从试管内流出。

为预防回流发生，请遵循以下预防措施：

（a） 在采集过程中请将病人的手臂保持向下姿势。

（b）手持试管时尽量将塞子向上。

（c）血液开始流入试管内或使用两分钟后解开止血带。

（d）在采集过程中，试管内物体不可与塞子或针接触。

2.遵循CLSI GP41-A61中所列的顺序进行抽血。

3.试管装满。

4.拔除试管上的连接管，并立刻轻轻倒转8至10次，使管内液体混合（如图示）。不充分混合或延迟混合均可导致不正确的检验结果。

 

 

5.采集完成后，请在指定温度范围内运输和储存。

6.根据仪器制造商的说明进行无细胞DNA BCT的提取。

注意：

A、STRECK无创管不会稀释血液，因此无需进行稀释因数校正来获取绝对数值。

B、正如大多数临床试验标本一样，溶血、黄疸和脂血均能影响Streck 218962 10mL Cell-Free DNA BCT Blood Collection Tube无创管中保存血样所得的检测结果。

 

【注意事项】

1、仅可用于科学研究，不可用于医学诊断。

2、装有样本的Streck 230253 9mL Hybrid Plastic Cell-Free DNA BCT Fusion2 第二代升级新型混合塑料采血管不可冷冻，以免试管发生破裂。

3、请在试管的有效期内使用。

4、不能用于采集人体注射用样本。

5、切勿稀释或添加其他成分。

6、试管灌装过量或偏低均可造成血液和添加剂比例失调，可导致分析结果不正确或降低产品性能。

    警告

    A、玻璃可能发生破裂，在处理过程中请做好预防措施。

    B、所有与产品接触的生物标本和材料都将被视为是具有传染性的生物公害，请按各地   相关法规进行处理。避免接触皮肤和粘膜。

    C、本品应按传染性医疗垃圾处理。

D、拔除或重新插入试管塞时，可轻轻摇晃塞子，或抓紧塞子的同时向外扭拉。切勿用拇指推出塞子，容易造成试管破裂而受伤。

 

无细胞血浆DNA和细胞基因组DNA的提取：

1、无细胞血浆DNA和细胞基因组DNA的提取可通过大部分市售试剂盒完成。

2、如欲获取zui佳结果，在60℃离液盐中加入一个蛋白酶K处理步骤（≥ 30 mAU/ml），提取无细胞DNA需1小时，提取细胞基因组DNA需2小时。

 

STRECK游离DNA采血管的使用限制：

1、仅供科研使用。

2、抽取进其他抗凝剂或防腐剂中的样本，在STRECK无创管中可能会导致凝结。

 

STRECK游离DNA采血管的参考：

临床实验室标准化委员会.CLSI GP41-A61，通过静脉穿刺术采集诊断血液样本程序.第六版认证标准.

 

现货供应：美国Streck 218962 Cell-Free DNA BCT Blood Collection Tube DNA基因检测无创采血管 和美国Streck 218976 Cell-Free RNA BCT Blood Collection Tube基因检测无创采血管 规格：100管/盒，应用于：唐氏无创筛查采血管，游离DNA采血管，游离RNA采血管等等。Streck 230253 9mL Hybrid Plastic Cell-Free DNA BCT Fusion2 第二代升级新型混合塑料采血管 现货供应：BD 362761 Vacutainer® CPT™ Cell Preparation Tube 肝素钠单核细胞制备管/采血管，60支/盒。

 ，  -：

提供产品：致力于为中国干细胞领域的干细胞科学研究、脐带血/造血干细胞/脐带/脂肪/胎盘干细胞库、干细胞临床转化医学、干细胞美容及干细胞抗衰老、免疫细胞治疗、T细胞治疗、临床干细胞治疗等再生医学和个体化治疗产业链的相关客户提供：MSC间充质干细胞培养基、iPS/ES胚胎干细胞培养基、血清替代物、羊水培养基、基底膜基质胶细胞支架、干细胞生长分化调控因子、BioMarker 流式检测抗体、支原体检测试剂盒、内毒素检测试剂盒、干细胞存储细胞冻存袋、细胞培养瓶、USP级DMSO二甲基亚砜细胞冻存液、细胞培养相关试剂耗材仪器等种类丰富的产品，以“Focus on improving the quality of Stem Cells专注干细胞研究，提升干细胞品质”为己任，我们与客户携手为中国干细胞产业的发展而前行。

 

CELL-FREE DNA BCT®

BLOOD COLLECTION TUBE

 

A blood collection tube which stabilizes nucleated blood cells. The unique preservative prevents the release of genomic DNA, allowing isolation of high-quality cell-free DNA. Now available in a unique hybrid plastic tube.

 

Convenient

Reduces the need for immediate plasma preparation and CTC processing.

Allows for isolation of high-quality cell-free DNA for a wide range of downstream applications.

Allows for convenient sample collection, transport and storage.

 

Stable

cfDNA and gDNA are stable for up to 14 days at 6℃to 37℃.

CTCs are stable for up to 7 days at 15℃to 30℃.

Unfilled tubes have 24-month shelf life from the date of manufacture.

 

Cell-Free DNA BCT is the trusted global leader in cell-stabilization.

 

Cell-Free DNA BCT® is for Research Use Only. Not for use in diagnostic procedures in the U.S.

 

A CE version of these tubes is also available.

Cell-Free DNA BCT CE is for Export Only. Not for sale in the U.S. This is also licensed for sale in Canada.

 

INSTRUCTIONS FOR USE

Cell-Free DNA BCT® is a direct draw whole blood collection tube intended for collection, transport and storage of blood samples. This product has not been cleared by the U.S. Food and Drug Administration for In Vitro Diagnostic use. The product is For Research Use Only. Not for use in diagnostic procedures.

SUMMARY AND PRINCIPLES

Cell-Free DNA BCT stabilizes cell-free plasma DNA as well as preserves cellular genomic DNA present in nucleated blood cells and circulating epithelial cells (tumor cells) found in whole blood.

Accurate analysis of cf-DNA can be compromised by sample handling, shipping and processing, causing lysis of nucleated blood cells and subsequent release of cellular genomic DNA. Additionally, degradation of cf-DNA due to nuclease activity can be problematic.

The preservative reagent contained in Cell-Free DNA BCTstabilizes nucleated blood cells, preventing the release of cellular genomic DNA, and inhibits nuclease mediated degradation of cf-DNA, contributing to the overall stabilization of cf-DNA. Samples collected in Cell-Free DNA BCT are stable for up to 14 days at temperatures between 6 °C to 37 °C, allowing convenient sample collection, transport and storage.

The preservative reagent contained in Cell-Free DNA BCT stabilizes circulating epithelial cells (tumor cells) in whole blood for up to 7 days at temperatures between 15 °C to 30 °C.

REAGENTS

Cell-Free DNA BCT contains the anticoagulant K3 EDTA and a cell preservative in a liquid medium.

PRECAUTIONS

1. For Research Use Only. Not for use in diagnostic procedures.

2. Do not freeze specimens collected in glass Cell-Free DNA BCT. (Streck part numbers: 218961, 218962,

218992, 230242, 230243)

3. Do not use tubes after expiration date.

4. Do not use tubes for collection of materials to be injected into patients.

5. Product is intended for use as supplied. Do not dilute or add other components to Cell-Free DNA BCT.

6. Overfilling or underfilling of tubes will result in an incorrect blood-to-additive ratio and may lead to incorrect analytic results or poor product performance.

CAUTION

a. Glass has the potential for breakage; precautionary measures should be taken during handling of glass tubes (Streck part numbers: 218961, 218962, 218992, 230242, 230243).

b. All biological specimens and materials coming in contact with them are considered biohazards and should be treated as if capable of transmitting infection. Dispose of in accordance with federal, state and local regulations. Avoid contact with skin and mucous membranes.

c. Product should be disposed with infectious medical waste.

d. Remove and reinsert stopper by either gently rocking the stopper from side to side or by grasping with a simultaneous twisting and pulling action. A “thumb roll” procedure for stopper removal is NOT recommended as tube breakage and injury may result.

e. A pediatric tube adapter is strongly recommended to better fit the 10.25mm diameter 2.0ml Cell-Free DNA BCT.

1.SDS can be obtained at www.streck。。ｃｏｍ or by calling .

STORAGE AND STABILITY

1. When stored at 2 °C to 30 °C, empty Cell-Free DNA BCT is stable through expiration date.

2. Short-term storage at 2 °C to 40 °C is acceptable for empty Cell-Free DNA BCT for up to 14 days.

3. Do not freeze empty Cell-Free DNA BCT. Proper insulation may be required for shipment during extreme temperature conditions.

4. Sample storage/stability

 

INDICATIONS OF PRODUCT DETERIORATION

1. Cloudiness or precipitate visible in reagent of empty tube.

2. If indications of product deterioration occur, contact Streck Technical Services at or technicalservices@streck.com.

 

INSTRUCTIONS FOR USE

For a video demonstration, visit www.streck。。ｃｏｍ/mixing.

1. Collect specimen by venipuncture according to CLSI GP41-A61.

Prevention of Backflow – Since Cell-Free DNA BCT contains chemical additives, it is important to avoid possible backflow from the tube.

To guard against backflow, observe the following precautions:

a. Keep patient’s arm in the downward position during the collection procedure.

b. Hold the tube with the stopper in the uppermost position so that the tube contents do not touch the stopper or the end of the needle during sample collection.

c. Release tourniquet once blood starts to flow in the tube, or within 2 minutes of application.

2.  Follow recommendations for order of draw outlined in CLSI GP41-A61. Cell-Free DNA BCT should be drawn after the EDTA tube and before the fluoride oxalate (glycolytic inhibitor) tube. If a Cell-Free DNA BCT tube immediay follows a heparin tube in the draw order, Streck recommends collecting a non-additive or EDTA tube as a waste tube prior to collection in the Cell-Free DNA BCT.

3. Fill tube compley.

4. Remove tube from adapter and immediay mix by gentle inversion 8 to 10 times. Inadequate or delayed mixing may result in incorrect analytical results or poor product performance. One inversion is a complete turn of the wrist, 180 degrees, and back per the figure below:

5. After collection, transport and store tubes within the recommended temperature range.

Note:

1. For best results, a 21G or 22G needle is advised. Slower fill times may be observed when using a smaller gauge needle.

2. When using a winged (butterfly) collection set for venipuncture and the Streck Cell-Free DNA BCT is the first tube drawn, a non-additive or EDTA discard tube should be partially drawn first in order to eliminate air or “dead space” from the tubing.

3. For the 2.0ml Cell-Free DNA BCT, care must be taken to center the tube so the middle of the stopper is punctured per the figure below. To aid with proper insertion of the tube in standard holders and stabilize the tube during the draw, a pediatric tube adapter is strongly recommended to modify the standard holder to fit the 10.25mm diameter 2.0ml Cell-Free DNA BCT (Streck part number: 230242, 230243).

4. Cell-Free DNA BCT does not dilute blood samples; therefore, no dilution factor correction is necessary.

5. As in the case with most clinical laboratory specimens, hemolysis, icterus and lipemia may affect the results obtained on blood samples preserved with Cell-Free DNA BCT.

 

DNA EXTRACTION

Extraction of cell-free plasma DNA and cellular genomic DNA can be accomplished using most commercially available kits that include a Proteinase K treatment step.

Cell-Free Plasma DNA

Streck has qualified two separate plasma separation spin protocols for your convenience.

Double Spin Protocol 1 

Step 1. To separate plasma, centrifuge whole blood at 300 x g for 20 minutes at room temperature.

Step 2. Remove the upper plasma layer and transfer to a new conical tube (not provided).

Step 3. Centrifuge the plasma at 5000 x g for 10 minutes.

Step 4. Isolate cell-free DNA per kit manufacturer instructions.

Double Spin Protocol 2 (for maximum plasma recovery)

Step 1. To separate plasma, centrifuge whole blood at 1600 x g for 10 minutes at room temperature.

Step 2. Remove the upper plasma layer and transfer to a new conical tube (not provided).

Step 3. Centrifuge the plasma at 16000 x g for 10 minutes.

Step 4. Isolate cell-free DNA per kit manufacturer instructions.

For optimal results, include a Proteinase K treatment step (≥ 30 mAU/mL digest) at 60 °C in the presence of chaotropic salts for 1 hour when extracting cell-free DNA.

 

Cellular Genomic DNA

Step 1. To separate the white blood cells, either lyse the red blood cells and wash, or centrifuge whole blood and collect the buffy coat layer.

Step 2. Isolate genomic DNA per kit manufacturer instructions.

For optimal results, include a Proteinase K treatment step (≥ 30 mAU/ml digest) at 60 °C in the presence of chaotropic salts for 2 hours when extracting cellular genomic DNA.

 

FREEZING AND THAWING PLASMA

1. To Freeze: For long-term storage, after spinning, collect and transfer the upper plasma layer to a cryogenic tube (not provided) and freeze at -20 ºC or -80 ºC.

2. To Thaw: Thaw cryogenic tubes at appropriate temperature as specified in your protocol.

Note: If cryoprecipitates form in the plasma, vortex the tube for 30 seconds after thawing. Do not centrifuge the plasma.

 

CONCENTRATED CELLULAR COMPLEMENT

After spinning and the upper plasma layer is collected and transferred to a cryogenic tube, the remaining cellular material in the plastic Cell-Free DNA BCT (Streck part numbers: 230252, 230253, 230254) may be frozen for future use.

1. To Freeze: For long-term storage, freeze the remaining cellular material vertically directly in the plastic CellFree DNA BCT at -20 ºC or -80 ºC.

2. To Thaw: Thaw tubes at appropriate temperature as specified in your protocol.

Note: A plastic Cell-Free DNA BCT, which has been compley filled with whole blood, should not be frozen;breakage may occur.

LIMITATIONS

1. For single use only.

2. Samples drawn in other anticoagulants or preservatives may cause coagulation in Cell-Free DNA BCT.

3. Specimen transport via pneumatic tube system is not advised.

 

REFERENCES

1. Clinical and Laboratory Standards Institute. GP41-A6, Procedures for the collection of diagnostic blood specimens by venipuncture. Approved Standard – Sixth Edition.

 

现货供应：美国Streck 218962 Cell-Free DNA BCT Blood Collection Tube DNA基因检测无创采血管 和美国Streck 218976 Cell-Free RNA BCT Blood Collection Tube基因检测无创采血管 规格：100管/盒，应用于：唐氏无创筛查采血管，游离DNA采血管，游离RNA采血管等等。Streck 230253 9mL Hybrid Plastic Cell-Free DNA BCT Fusion2 第二代升级新型混合塑料采血管 现货供应：BD 362761 Vacutainer® CPT™ Cell Preparation Tube 肝素钠单核细胞制备管/采血管，60支/盒。

 ，  -：

提供产品：致力于为中国干细胞领域的干细胞科学研究、脐带血/造血干细胞/脐带/脂肪/胎盘干细胞库、干细胞临床转化医学、干细胞美容及干细胞抗衰老、免疫细胞治疗、T细胞治疗、临床干细胞治疗等再生医学和个体化治疗产业链的相关客户提供：MSC间充质干细胞培养基、iPS/ES胚胎干细胞培养基、血清替代物、羊水培养基、基底膜基质胶细胞支架、干细胞生长分化调控因子、BioMarker 流式检测抗体、支原体检测试剂盒、内毒素检测试剂盒、干细胞存储细胞冻存袋、细胞培养瓶、USP级DMSO二甲基亚砜细胞冻存液、细胞培养相关试剂耗材仪器等种类丰富的产品，以“Focus on improving the quality of Stem Cells专注干细胞研究，提升干细胞品质”为己任，我们与客户携手为中国干细胞产业的发展而前行。