Anti-Clathrin heavy chain antibody [TD.1] (ab24578)反网络蛋白重链抗体
货号：ab24578
厂家：Abcam公司
规格：100ul
保存温度：-20℃ 和 2～8℃
货期：现货2～5天，期货2-3周
Anti-Clathrin heavy chain antibody [TD.1] (ab24578)反网络蛋白重链抗体产品信息：
- Product nameAnti-Clathrin heavy chain antibody [TD.1]See all Clathrin heavy chain primary antibodies …
- Description
Mouse monoclonal [TD.1] to Clathrin heavy chain
- SpecificityThis antibody reacts with clathrin heavy chains (CHC).
- Tested applicationsIHC-P, WB, ICC/IF
- Species reactivity
Reacts with: Cow, Human
- Immunogen
This antibody was raised using the globular N terminal domain of clathrin heavy chain.
- Positive control
- Methanol fixed HeLa cells for IF.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.03% Thimerosal (merthiolate).
Constituents: PBS. -
Concentration 100 µl at 1 mg/ml
- PurityProtein G purified
- Clonality Monoclonal
- Clone numberTD.1
- IsotypeIgG1
- Research Areas
- Signal Transduction
- Protein Trafficking
- Vesicle Transport
- Coat Proteins
- Tags & Cell Markers
- Subcellular Markers
- Organelles
- Caveolae and Clathrin
Applications
Our Abpromise guarantee covers the use of ab24578 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IHC-P | IHC-P: Use at an assay dependent concentration. PubMed: 21571834 |
| WB | WB: 1/250. Predicted molecular weight: 192 kDa. |
| ICC/IF | ICC/IF: Use at an assay dependent dilution. |
- Application notesIs unsuitable for or IP.
Target
- FunctionClathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.
- Sequence similaritiesBelongs to the clathrin heavy chain family.
- Cellular localizationCytoplasmic vesicle membrane. Membrane > coated pit. Melanosome. Cytoplasmic face of coated pits and vesicles. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
-
Database links
- Entrez Gene: 281080 Cow
- Entrez Gene: 1213 Human
- Omim: 118955 Human
- SwissProt: P49951 Cow
- SwissProt: P53675 Human
- SwissProt: Q00610 Human
- Unigene: 491351 Human
-
Alternative names
- CHC17 antibody
- Clathrin heavy chain 1 antibody
- Clathrin heavy chain 2 antibody
Anti-Clathrin heavy chain antibody [TD.1] images
Immunocytochemistry/ Immunofluorescence – Clathrin heavy chain antibody [TD.1] (ab24578)
ab24578 at a 1/200 dilution staining clathrin triskelion (blue) throughout the methanol fixed HeLa cells by Immunocytochemistry/Immunofluorescence. The Golgi is brilliantly stained by Giantin (yellow). Secondary antibodies used were a goat anti-mouse alexafluor AF546 and a goat anti-rabbit alexafluor AF488 applied at 1 ug/mL. The DNA was visualized with DAPI at 1 ug/mL. The colors of this image were altered.
公司介绍：
Abcam位于英国的剑桥科学园，成立于1998年，专门生产和分销研究型抗体。主要创立人Jonathan Milner和实验室同事构思成立一间了解科研人员需要的网上抗体公司，理念是Abcam销售质量高的抗体，并提供zui全面、诚实、时新的说明书，快速的送货服务，及高效率的顾客和服务。我们的在线目录(www.hystemcell.cn) 已有差不多100,000种抗体和试剂，并不断添加，供应予全球百多个国家。Abcam于2005年11月在伦敦证券交易所上市，在美国马萨诸塞州、日本东京及香港均设有分公司。
Abcam 一直致力加强产品线，为使研究员更容易找到蛋白质研究试剂产品，在2011并购了美国的MitoSciences公司，加强了免疫分析方面的产品供应；同年也并购了英国的Ascent Scientific 公司，开展了生化试剂的供应。在2012年，Abcam并购了美国的Epitomics 公司，成为一家有领导地位的RabMAbs® 供应商。
Abcam 的 目标是给世界上zui好的抗体建立zui大的在线目录，为各地科学家提供尖端产品，成为各国科学界的重要伙伴。我们为所有产品提供来使客户获得预期的结 果。为提供高质量的抗体来指向尽可能多的靶蛋白，我们尽所能在尽可能多的应用和物种中检测每种抗体。我们相信诚信才是上策，在我们的上会发布有关我们 每一种产品的尽可能多的信息。
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Abcam ab1267Goat polyclonal to Glu-Glu tag （HRP）100ug3996Glu-Glu taghystemcell一抗
Abcam ab12675Rabbit polyclonal to GPCR MCH250ug3996GPCR MCH2hystemcell一抗
Abcam ab12678Rabbit polyclonal to GPR 17950ug4940GPCR AGR9hystemcell一抗
Abcam ab1268Goat polyclonal to Glu-Glu tag （FITC）250ug3996Glu-Glu taghystemcell一抗
Abcam ab12680Rabbit polyclonal to D650ug3996GPCR D6hystemcell一抗
