ab15148-Anti-E Cadherin antibody （ab15148）抗E钙黏蛋白抗体-抗体/抗原

发表于由

Anti-E Cadherin antibody (ab15148)抗E钙黏蛋白抗体
货号：ab15148
厂家：Abcam公司
规格：500ul
保存温度：-20℃ 和 2～8℃
货期：现货2～5天，期货2-3周

咨询：  （重庆） （贵阳）

Anti-E Cadherin antibody (ab15148)抗E钙黏蛋白抗体产品信息：

  • Product nameAnti-E Cadherin antibody
  • Description

    Rabbit polyclonal to E Cadherin

  • SpecificityWe have data to indicate that this antibody may not cross react with Chicken. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsWB, ICC/IF, ELISA, ICC, IHC-P, IHC-Fr
  • Species reactivity

    Reacts with: Human, Pig

  • Immunogen

    Recombinant fragment: IFFCERNPKP QVINIIDADL PPNTSPFTAE LTHGASANWT IQYNDPTQES IILKPKMALE VGDYKINLKL MDNQNKDQVT TLEVSVCDCE GAAGVCRKAQ PVEAGLQI , corresponding to amino acids 600-707 of Human E Cadherin.

  • Positive control
    • Skin

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: Sodium Azide
    Constituents: 10mM PBS, BSA. pH 7.4

  • Concentration 500 µl at 0.2 mg/ml

  • PurityImmunogen affinity purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cadherins
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Binding Proteins
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Cadherins
    • Developmental Biology
    • Organogenesis
    • Excretory system development
    • Kidney development

Applications

Our Abpromise guarantee covers the use of ab15148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/500. Detects a band of approximay 120 kDa.
ICC/IF ICC/IF: Use at an assay dependent concentration.
ELISA ELISA: Use at an assay dependent concentration.
ICC ICC: Use at an assay dependent dilution.
IHC-P IHC-P: 1/30. for 10 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
IHC-Fr IHC-Fr: 1/50.

Target

  • FunctionCadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
    E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.
  • Tissue specificityNon-neural epithelial tissues.
  • Involvement in diseaseDefects in CDH1 are the cause of hereditary diffuse gastric cancer (HDGC) [MIM:137215]. An autosomal dominant cancer predisposition syndrome with increased susceptibility to diffuse gastric cancer. Diffuse gastric cancer is a malignant disease characterized by poorly differentiated infiltrating lesions resulting in thickening of the stomach. Malignant tumors start in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. Note=Heterozygous germline mutations CDH1 are responsible for familial cases of diffuse gastric cancer. Somatic mutations in the has also been found in patients with sporadic diffuse gastric cancer and lobular breast cancer.
    Defects in CDH1 are a cause of susceptibility to endometrial cancer (ENDMC) [MIM:608089].
    Defects in CDH1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
  • Sequence similaritiesContains 5 cadherin domains.
  • Post-translational
    modificationsDuring apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions.
  • Cellular localizationCell junction. Cell membrane. Endosome. Golgi apparatus > trans-Golgi network. Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane.

  • Database links

    • Entrez Gene: 999 Human
    • Entrez Gene: 100048953 Pig
    • Omim: 192090 Human
    • SwissProt: P12830 Human
    • Unigene: 461086 Human

    • Alternative names

      • Arc 1 antibody
      • CADH1_HUMAN antibody
      • Cadherin 1 antibody

    Anti-E Cadherin antibody images

    • ab15148-Anti-E Cadherin antibody （ab15148）抗E钙黏蛋白抗体-抗体/抗原

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) – E Cadherin antibody (ab15148)

      Immunohistochemical staining of formalin fixed paraffin embedded human skin using ab15148.

    • ab15148-Anti-E Cadherin antibody （ab15148）抗E钙黏蛋白抗体-抗体/抗原

      Immunocytochemistry/ Immunofluorescence – E Cadherin antibody (ab15148)This image is courtesy of an Abreview submitted by Dr Mauricio Pinto

      ab15148 staining human MCF10A cells by ICC/IF.  Cells were fixed with paraformaldehyde and blocked using 10% serum for 30 minutes at 25 °C.  The primary antibody was diluted 1/25 in TBST and incubated for 1 hour at 25 °C.  An Alexa Fluor® 555 goat anti-rabbit was used as the secondary antibody.

    • ab15148-Anti-E Cadherin antibody （ab15148）抗E钙黏蛋白抗体-抗体/抗原

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) – E Cadherin antibody (ab15148)This image is a courtesy of Anonymous Abreview

      ab15148 staining E Cadherin in human stomach tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before heat mediated antigen retrieval in Citrate pH 6.0 and then blocking with 5% serum for 1 hour at 23°C was performed. The primary antibody was used diluted 1/50 and incubated with sample for 1 hour at 23°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary antibody.

    • ab15148-Anti-E Cadherin antibody （ab15148）抗E钙黏蛋白抗体-抗体/抗原

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) – E Cadherin antibody (ab15148)This image is courtesy of an anonymous Abreview

      ab15148 staining E Cadherin in Pig Cervix uteri tissue sections by IHC-P (Formaldehyde-fixed, Paraffin-embedded sections). Tissue samples were fixed with formaldehyde and blocked with 10% goat serum for 1 hour at 37°C; antigen retrieval was by heat mediation in 10mM citrate at pH 6 for 2 minutes. The sample was incubated with primary antibody (1/50) at 4°C for 12 hours. An HRP-conjugated goat polyclonal to rabbit IgG (undiluted) was used as secondary antibody.

    • ab15148-Anti-E Cadherin antibody （ab15148）抗E钙黏蛋白抗体-抗体/抗原

      Immunocytochemistry/ Immunofluorescence – E Cadherin antibody (ab15148)This image is courtesy of an anonymous Abreview

      ab15148 staining E cadherin in Human AGS Gastric carcinoma cultured cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilzed with 0.025% Trton X-100 in TBS and blocked with 5% serum for 1 hour at 23°C. Samples were incubated with primary antibody (1/50 in blocking buffer) for 1 hour at 23°C. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    • ab15148-Anti-E Cadherin antibody （ab15148）抗E钙黏蛋白抗体-抗体/抗原

      Immunohistochemistry (Frozen sections) – E Cadherin antibody (ab15148)This image is courtesy of an anonymous Abreview.

      ab15148 staining E Cadherin in Human AGS Gastric Carcinoma tissue sections by Immunohistochemistry (Frozen sections). The sections were acetone fixed and blocked in 5% serum for 1 hour at 23°C. The primary antibody was diluted 1/50 in blocking buffer and incubated with the sample for 1 hours at 23°C.  An HRP-conjugated Goat polyclonal to Rabbit IgG, diluted 1/200, was used as the secondary.

    • ab15148-Anti-E Cadherin antibody （ab15148）抗E钙黏蛋白抗体-抗体/抗原
    • Western blot – E Cadherin antibody (ab15148)

      All lanes : Anti-E Cadherin antibody (ab15148) at 1/500 dilution (for 16 hours at 4°C)
      Lane 1 : Human OE33 cell – whole cell lysate
      Lane 2 : Human OE33 cell – whole cell lysate
      Lysates/proteins at 20 µg per lane.
      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/1000 dilution
      developed using the ECL technique
      Performed under reducing conditions.
      Observed band size : 120 kDa

    • 公司介绍：

          Abcam位于英国的剑桥科学园，成立于1998年，专门生产和分销研究型抗体。主要创立人Jonathan Milner和实验室同事构思成立一间了解科研人员需要的网上抗体公司，理念是Abcam销售质量高的抗体，并提供zui全面、诚实、时新的说明书，快速的送货服务，及高效率的顾客和服务。我们的在线目录(www.hystemcell.cn) 已有差不多100,000种抗体和试剂，并不断添加，供应予全球百多个国家。Abcam于2005年11月在伦敦证券交易所上市，在美国马萨诸塞州、日本东京及香港均设有分公司。

          Abcam 一直致力加强产品线，为使研究员更容易找到蛋白质研究试剂产品，在2011并购了美国的MitoSciences公司，加强了免疫分析方面的产品供应；同年也并购了英国的Ascent Scientific 公司，开展了生化试剂的供应。在2012年，Abcam并购了美国的Epitomics 公司，成为一家有领导地位的RabMAbs® 供应商。

          Abcam 的 目标是给世界上zui好的抗体建立zui大的在线目录，为各地科学家提供尖端产品，成为各国科学界的重要伙伴。我们为所有产品提供来使客户获得预期的结 果。为提供高质量的抗体来指向尽可能多的靶蛋白，我们尽所能在尽可能多的应用和物种中检测每种抗体。我们相信诚信才是上策，在我们的上会发布有关我们 每一种产品的尽可能多的信息。

      更多产品：

      Abcamab1132Mouse monoclonal [1718] to Thyroid Hormone Receptor100ul3996Thyroid Hormone Receptor抗体一抗

      Abcamab11320Rabbit polyclonal to gamma Tubulin – Centrosome Marker100ul3996gamma Tubulin抗体一抗

      Abcamab11321Rabbit polyclonal to gamma Tubulin – Centrosome Marker100ul3996gamma Tubulin抗体一抗

      Abcamab11322Mouse monoclonal [TUB-11] to epsilon Tubulin100ul3996epsilon Tubulin抗体一抗

      Abcamab11323Mouse monoclonal [6-11B-1] to Tubulin （acetyl K40）100ul3996Tubulin抗体一抗

      Abcamab11324Mouse monoclonal [B3] to Tubulin – Polyglutamylated100ul4274Tubulin抗体一抗

      Abcamab11325Mouse monoclonal [TUB-1A2] to Tubulin100ul3996Tubulin抗体一抗

      Abcamab11326Mouse monoclonal [100/2] to alpha Adaptin100ul3996alpha Adaptin抗体一抗

      Abcamab11327Mouse monoclonal [100/1] to AP1+2 beta1 00ul3996beta 1/2 Adaptin抗体一抗

      Abcamab11328Mouse monoclonal [100/3] to gamma Adaptin100ul3996gamma Adaptin抗体一抗

    • Abcamab12793RNA polymerase II CTD repeat YSPTSPS peptide – phospho S2100ug3286RNA polymerase II CTD repeat YSPTSPS蛋白/多肽多肽

      Abcamab12795RNA polymerase II CTD repeat YSPTSPS peptide100ug2010RNA polymerase II CTD repeat YSPTSPS蛋白/多肽多肽

      Abcamab12797Rabbit polyclonal to GPCR ORF450ug3996GPCR ORF4hystemcell一抗

      Abcamab12798Rabbit polyclonal to 5-oxo-ETE GPCR50ug4940GPCR TG1019hystemcell一抗

      Abcamab1280Mouse IgG1 （HybIgG1） （Phycoerythrin） – Isotype control1ml3996Mouse IgG1hystemcell同型对照

      Abcamab12800Rabbit polyclonal to GPCR TM7SF150ug3996GPCR TM7SF1hystemcell一抗

      Abcamab12801Rabbit polyclonal to GPR5650ug3996GPR56hystemcell一抗

      Abcamab12807Rabbit polyclonal to GPCR GPR10350ug4940GPCR GPR103hystemcell一抗

      Abcamab1281Mouse IgG2a （HybIgG2a） （FITC） （HybIgG2a） – isotype control1ml3996Mouse IgG2ahystemcell同型对照

      Abcamab12815Rabbit polyclonal to GPCR GPR12850ug4940GPCR GPR128hystemcell一抗

      Abcamab12818Rabbit polyclonal to GPCR GPR2250ug4940GPCR GPR22hystemcell一抗

      Abcamab1282Mouse IgG2a monoclonal [HybIgG2a] （Phycoerythrin） – isotype control1ml3996Mouse IgG2ahystemcell同型对照

      Abcamab12820Rabbit polyclonal to GPCR GPR2750ug3996GPCR GPR27hystemcell一抗

      Abcamab12827Rabbit polyclonal to GPCR GPR4950ug5029GPCR GPR49hystemcell一抗

      Abcamab1283Mouse IgG2b （HybIgG2b） （FITC） – Isotype control100tests3996Mouse IgG2bhystemcell同型对照

      Abcamab12834Rabbit polyclonal to GPCR GPR7850ug4940GPCR GPR78hystemcell一抗

      Abcamab1284Mouse IgG2b monoclonal [HybIgG2b] （Phycoerythrin） – isotype control1ml3996Mouse IgG2bhystemcell同型对照

      Abcamab12842Rabbit polyclonal to GPCR GPRC5B50ug3996GPCR GPRC5Bhystemcell一抗

      Abcamab12843Rabbit polyclonal to GPCR GPRC5D50ug4940GPCR GPRC5Dhystemcell一抗

      Abcamab12847Rabbit polyclonal to GPR 13950ug3996GPCR LS190419hystemcell一抗

      Abcamab1285Mouse IgG1 monoclonal [HybIgG1] （FITC+Phycoerythrin） – isotype control50tests3996Mouse IgG1hystemcell同型对照

      Abcamab12855Rabbit polyclonal to GPCR MRGE50ug3996GPCR MRGEhystemcell一抗

      Abcamab12857Rabbit polyclonal to GPCR MRGX250ug3996GPCR MRGX2hystemcell一抗

      Abcamab12861Rabbit polyclonal to Casein Kinase 2 beta （phospho S209）50ul3996Casein Kinase 2 betahystemcell一抗

      Abcamab12862Rabbit polyclonal to Syk （phospho Y323）50ul3996Sykhystemcell一抗

      Abcamab12863Rabbit polyclonal to GAB1 （phospho Y627）50ul3996GAB1hystemcell一抗

      Abcamab12864Rabbit polyclonal to RPS6 （phospho S235+S236）50ul3996RPS6hystemcell一抗

      Abcamab12865Rabbit polyclonal to RPS6 （phospho S244+S247）50ul3996RPS6hystemcell一抗

      Abcamab12866Rabbit polyclonal to Cofilin （phospho S3）50ul4274Cofilinhystemcell一抗

      Abcamab12868Rabbit polyclonal to ZAP70 （phospho Y292）50ul3996ZAP70hystemcell一抗

      Abcamab12869Rabbit polyclonal to ZAP70 （phospho Y315+Y319）50ul3996ZAP70hystemcell一抗

      Abcamab1287Rabbit polyclonal to Aurora A50ug3996Aurora Ahystemcell一抗

      Abcamab12873Rabbit polyclonal to MCHR50ug4940GPCR SLC/MCH1hystemcell一抗

      Abcamab12874Rabbit polyclonal to GPR5650ug3996GPR56hystemcell一抗

      Abcamab12875Rabbit polyclonal to Aurora A100ug4418Aurora Ahystemcell一抗

      Abcamab12894Rabbit polyclonal to Mannose 6 Phosphate Receptor （Cation independent） – Late Endosome Marker100ug3996Mannose 6 Phosphate Receptor （Cation independent）hystemcell一抗

      Abcamab1291Rabbit polyclonal to BRCA1 （Agarose）100ug5029BRCA1hystemcell一抗

      Abcamab12916Rabbit polyclonal to NR1D150ug4607NR1D1hystemcell一抗

      Abcamab1292Rabbit polyclonal to BRCA2 （Agarose）50ug3996BRCA2hystemcell一抗

      Abcamab12923Rabbit polyclonal to alpha 1d Adrenergic Receptor50ug4607alpha 1d Adrenergic Receptorhystemcell一抗

      Abcamab12925Mouse monoclonal [GP-3] to RASA3100ul3996RASA3hystemcell一抗

      Abcamab12926Rabbit polyclonal to 5HT2B Receptor50ug39965HT2B Receptorhystemcell一抗

      Abcamab12929Rabbit polyclonal to Histone H2B （mono methyl K5） -ChIP Grade50ug3996Histone H2Bhystemcell一抗

      Abcamab1293Rabbit polyclonal to MKLP1 （Agarose）100ug5029MKLP1hystemcell一抗

      Abcamab12935Rabbit polyclonal to alpha 1c Adrenergic Receptor50ug4940alpha 1c Adrenergic Receptorhystemcell一抗

      Abcamab1294Rabbit polyclonal to MSH2 （Agarose）100ug3996MSH2hystemcell一抗

      Abcamab12945Rabbit polyclonal to BDKRB150ug3996BDKRB1hystemcell一抗

      Abcamab12947Rabbit polyclonal to BDKRB225ug3996BDKRB2hystemcell一抗

      Abcamab1295Rabbit polyclonal to MLH1 （Agarose）100ug5029MLH1hystemcell一抗

      Abcamab12950Rabbit polyclonal to Histone H2A （mono methyl K127）50ug2664Histone H2Ahystemcell一抗

      Abcamab12952Histone H2A peptide – mono methyl K127100ug2010Histone H2A蛋白/多肽多肽

      Abcamab12958Rabbit polyclonal to Flamingo homolog 150ug4940Flamingo homolog 1hystemcell一抗

      Abcamab1296Rabbit polyclonal to SMC1 （Agarose）100ug3885SMC1hystemcell一抗

      Abcamab12962Rabbit polyclonal to C5R150ug3996C5R1hystemcell一抗

      Abcamab12964Rabbit polyclonal to Corticotropin Releasing Factor Receptor 250ug5029Corticotropin Releasing Factor Receptor 2hystemcell一抗

      Abcamab1297Rabbit polyclonal to SMC3 （Agarose）50ug3996SMC3hystemcell一抗

      Abcamab12974Rabbit polyclonal to EMR350ug3996EMR3hystemcell一抗

      Abcamab12977Rabbit polyclonal to Endothelin A Receptor50ug4773Endothelin A Receptorhystemcell一抗

      Abcamab1298Mouse monoclonal [mAbcam 1298] to Histone H3 （di methyl K9）100ug3996Histone H3hystemcell一抗

      Abcamab12986Rabbit polyclonal to Estrogen Related Receptor beta50ug3996Estrogen Related Receptor betahystemcell一抗

      Abcamab12989Rabbit polyclonal to ETL50ug3996ETLhystemcell一抗

      Abcamab1299Mouse monoclonal [NN 2C10/1] to GM130 – Golgi Marker100ug3996GM130hystemcell一抗

      Abcamab12990Rabbit polyclonal to FPR150ug4940FPR1hystemcell一抗

      Abcamab12994Rabbit polyclonal to Frizzled 650ug4940Frizzled 6hystemcell一抗

      Abcamab13001Rabbit polyclonal to GALR150ug3996GALR1hystemcell一抗

      Abcamab13003Rabbit polyclonal to GALR350ug4940GALR3hystemcell一抗

      Abcamab13004Rabbit polyclonal to Gastric Inhibitory Polypeptide Receptor50ug4607Gastric Inhibitory Polypeptide Receptorhystemcell一抗

      Abcamab13008Aurora A peptide100ug2010Aurora A蛋白/多肽多肽

      Abcamab1301Goat polyclonal to LASP1100ug3996LASP1hystemcell一抗

      Abcamab13011Rabbit polyclonal to GnRHR50ug4940GnRHRhystemcell一抗

      Abcamab13015Rabbit polyclonal to HRH325ug3996HRH3hystemcell一抗

      Abcamab13016Rabbit polyclonal to HUNK50ug3996HUNKhystemcell一抗

      Abcamab1302Goat polyclonal to CrkL100ug3996CrkLhystemcell一抗

      Abcamab13022Rabbit polyclonal to LPHN350ug4940LPHN3hystemcell一抗

      Abcamab13025Rabbit polyclonal to GPR10850ug4940GPR108hystemcell一抗

      Abcamab13026Rabbit polyclonal to EDG250ug3996EDG2hystemcell一抗

      Abcamab13027Rabbit polyclonal to EDG225ug3996EDG2hystemcell一抗

      Abcamab13028Rabbit polyclonal to EDG450ug3996EDG4hystemcell一抗

      Abcamab13033Rabbit polyclonal to MC1 Receptor50ug4940MC1 Receptorhystemcell一抗

      Abcamab13034Rabbit polyclonal to MC4 Receptor50ug3996MC4 Receptorhystemcell一抗

      Abcamab13037Rabbit polyclonal to Metabotropic Glutamate Receptor 150ug3996Metabotropic Glutamate Receptor 1hystemcell一抗

      Abcamab1304Mouse monoclonal [10] to Vascular Endothelium100ug3996Vascular Endotheliumhystemcell一抗

      Abcamab13040Rabbit polyclonal to Metabotropic Glutamate Receptor 250ug3996Metabotropic Glutamate Receptor 2hystemcell一抗

      Abcamab13041Rabbit polyclonal to Metabotropic Glutamate Receptor 350ug3996Metabotropic Glutamate Receptor 3hystemcell一抗

      Abcamab13042Rabbit polyclonal to Metabotropic Glutamate Receptor 750ug3996Metabotropic Glutamate Receptor 7hystemcell一抗

      Abcamab11329Mouse monoclonal [AP180-I] to AP180100ul3996AP18抗体一抗

      Abcamab1133Rabbit polyclonal to GABARAP100ul3996GABARAP抗体一抗

      更多详情，请：