Toll样受体配体之炎症小体诱导剂

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Toll样受体配体之炎症小体诱导剂

炎症小体是一个多蛋白复合物，通常由NLR家族的成员之一，如NLRP3、NLRC4，与接头蛋白ASC(包含CARD结构域的凋亡相关斑点样蛋白)，以及Caspase-1前体蛋白这三种分子所组成。天然免疫细胞感受到细胞浆中的病原相关模式分子(PAMP)或危险相关模式分子(DAMP)后，在其细胞浆内开始组装炎症小体，进而促进炎症因子IL-1和IL-18的释放，引起快速的炎症相关细胞死亡，即pyrotosis。

研究最为广泛的是NLRP3炎症小体，它可以被很多刺激物诱导形成，包括危险信号(如ATP)、结晶质(如MSU)和微生物毒素(如尼日利亚菌素，即nigericin)。NLRC4(包含Caspase活化及募集结构域的NLR4)/NAIP(神经凋亡抑制性蛋白)炎症小体是由Salmonella typhimurium(鼠伤寒沙门氏杆菌)、Pseudomonas aeruginosa(绿浓杆菌)等细菌的鞭毛蛋白释放到细胞质中所引起的。NRLP1炎症小体则是由Bacillus anthracis lethal toxin(炭疽致死毒素)、Toxoplasma gondii(刚地弓形虫)、muramyl dipeptide(胞壁酰双肽)和宿主胞内ATP耗盡等诸多刺激诱导产生的，进而促进Caspase-1的生成。

炎症小体诱导剂相关产品：

PRODUCT

ORIGIN/DESCRIPTION

GRADE*

WORKING

CONCENTRATION

QTY

CATALOG

CODE

INFLAMMASOME INDUCERS

AIM2 Inflammasome Agonists

Poly(dA:dT) Naked

Poly(dA-dT)•poly(dT-dA)

TLR2 -/ TLR4 –

10 ng/ml – 1 µg/ml

200 µg

1 mg

tlrl-patn

tlrl-patn-1

Poly(dA:dT)/LyoVec

Poly(dA-dT)•poly(dT-dA)/LyoVec™ complexes

TLR2 -/ TLR4 –

10 ng – 10 µg/ml

100 µg

tlrl-patc

Poly(dA:dT)/Rhodamine

Rhodamine labeled poly(dA-dT)•poly(dT-dA)

TLR2 -/ TLR4 –

100 ng – 10 µg/ml

10 µg

tlrl-patrh

 

PRR Ligands

 

PRODUCT

ORIGIN/DESCRIPTION

GRADE*

WORKING

CONCENTRATION

QTY

CATALOG

CODE

NLRP1 Inflammasome Inducers

L18-MDP

Muramyldipeptide with a C18 fatty acid chain

TLR2 -/ TLR4 –

1 – 100 ng/ml

1 mg

tlrl-lmdp

MDP

Muramyldipeptide (L-D isoform, active)

TLR2 -/ TLR4 –

10 ng – 10 µg/ml

5 mg

tlrl-mdp

NLRP3 Inflammasome Inducers

Alum Crystals

Aluminium potassium sulfate

TLR2 -/ TLR4 –

10 – 200 µg/ml

1 g

tlrl-alk

ATP

Adenosine 5’-triphosphate disodium salt

TLR2 -/ TLR4 –

5 mM

1 g

tlrl-atpl

CPPD Crystals

Calcium pyrophosphate dihydrate

TLR2 -/ TLR4 –

1 – 50 µg/ml

5 mg

tlrl-cppd

Hemozoin

Synthetic heme crystal

TLR2 -/ TLR4 –

50 – 400 µg/ml

5 mg

tlrl-hz

MSU Crystals

Monosodium urate (uric acid)

TLR2 -/ TLR4 –

50 – 200 µg/ml

5 mg

25 mg

tlrl-msu

tlrl-msu-25

Nano-SiO2

Nanoparticles of silica dioxide

TLR2 -/ TLR4 –

10 – 200 µg/ml

10 mg

tlrl-sio

Nigericin

Nigericin, sodium salt

TLR2 -/ TLR4 –

1 µM

10 mg

50 mg

tlrl-nig

tlrl-nig-5

TDB

Synthetic analog of the cord factor

TLR2 -/ TLR4 –

1 – 100 µg/ml

2 mg

tlrl-tdb

Non-canonical Inflammasome Inducers

E. coli OMVs             NEW          

Purified outer membrane vesicles from E. coli BL21

TLR2 +/ TLR4 +

0.2-100 µg/ml

100 µg

tlrl-omv-1

E. coli OMVs InvivoFit™  NEW

Sterile grade of outer membrane vesicles from E. coli

TLR2 +/ TLR4 +

10-100 µg/mouse

500 µg

tlrl-omv

Curdlan

Beta-1,3-glucan from Alcaligenes faecalis

TLR2 -/ TLR4 –

0.1 – 100 µg/ml

1 g

tlrl-curd

HKCA

Heat-killed Candida albicans

TLR2 -/ TLR4 –

108 cells/ml

109 cells

tlrl-hkca

Pustulan

Beta-glucan from Lasallia pustulata

TLR2 -/ TLR4 –

0.1 – 100 µg/ml

100 mg

tlrl-pst

Zymosan Depleted

Hot alkali treated zymosan

TLR2 -/ TLR4 –

100 µg/ml

10 mg

tlrl-zyd

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